共查询到18条相似文献,搜索用时 70 毫秒
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超声波辅助提取狼毒大戟中二萜类物质Jolkinolide B 总被引:1,自引:0,他引:1
以w(C2H5OH)=95%的乙醇为提取剂,HPLC法测定的Jolkinolide B提取率为指标,考察了利用超声波辅助技术提取狼毒大戟中二萜类物质的适宜提取条件。测定出狼毒大戟中w(Jolkinolide B)=0.0216%。考察了提取时间、提取温度、料液质量比等因素对Jolkinolide B提取率的影响。室温下,提取时间从10 min到60 min,Jolkinolide B的提取率由0.014%提高至0.0149%;温度从室温到85℃,提取率从0.014%提高到0.020 5%;超声波辅助提取10 min,提取率与浸泡提取200 h的提取率相当;超声波辅助提取中适宜的m(狼毒大戟)∶m(提取剂)=1∶8。该文报告工作的新颖性已为内蒙古自治区科学技术信息技术研究所2007年3月12日出具的第20071500100031号《科技查新报告》所证实。 相似文献
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以银杏叶提取物生产水沉废渣为原料,研究大孔树脂柱层析纯化其银杏酚酸的工艺。以总银杏酚酸为指标采用静态吸附实验对5种大孔吸附树脂进行筛选,动态实验筛选上样流速、洗脱剂浓度、洗脱剂体积、树脂柱径高比参数,最后采用HPLC进行结果检测分析。结果确定HPD-5000大孔树脂为吸附分离树脂,上样流速1 BV/h,洗脱剂乙醇浓度为70%除杂,90%洗脱,90%乙醇洗脱剂体积为4 BV,树脂径高比1∶6。树脂柱纯化后提取浸膏中银杏酚酸含量由原料17%提高至77.87%。经验证实验,HPD-5000大孔树脂纯化后银杏酚酸富集效果明显,操作简单,周期短,树脂可重复利用,有利于银杏叶药材资源综合开发利用及生产。 相似文献
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针对人参水提取液脂质体,采用薄膜分散一冻融法制备,标准加入法测定人参水提取液中人参皂甙的含量,并测定了脂质体的包封率。实验结果表明,用标准加入法测定人参水提取液中人参皂甙的含量,方法简便、准确。用标准加入法测定得人参水提取液脂质体的包封率为37.26%。 相似文献
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超临界CO2萃取灵香草精油及其化学成分研究 总被引:9,自引:1,他引:8
用超临界CO2萃取技术(SFE-CO2),对灵香草(Lysimachia foenum-graecum Hance)进行了萃取,得到灵香草萃取物。探讨了萃取压力、时间及温度等因素对萃取收率的影响,较佳的工艺为:压力25 MPa、温度40℃、时间4 h。超临界CO2法得到的灵香草萃取物收率为4.5%,高于水蒸气法的收率,且香气纯正。采用GC-MS技术对SFE-CO2法得到的灵香草萃取物化学组成进行了分析鉴定,共鉴定出37个化学成分,总质量分数为91.91%,其中主要成分为:苯乙酮、(Z,Z,Z)-9,12,15-十八碳三烯酸甲酯、(Z,Z)-9,12-十八碳二烯酸、9-十六碳烯酸、十七酸、十七酸乙酯、(Z,Z,Z)-9,12,15-十八碳三烯酸乙酯等化合物。 相似文献
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《分离科学与技术》2012,47(11):1745-1751
A method based on an offline coupling of high-performance liquid chromatography/electrospray ionization multi-stage mass spectrometry (HPLC-ESI-MSn) and high-speed counter-current chromatography (HSCCC), was established for targeted separation and purification of skin-irritative and structurally similar ingenol-type diterpenoids in Euphorbia kansui. The crude dichloromethane extract of Euphorbia kansui after an initial clean-up step on silica gel and targeted fractionation according to HPLC-ESI-MSn, was separated by HSCCC. As a result, 3-O-(2′E,4′Z-decadienoyl)-20-O-acetylingenol and 3-O-(2′E,4′E-decadienoyl)-20-O-acetylingenol as well as a pair of geometrical isomers, 5-O-(2′E,4′Z-decadienoyl)-20-O-acetylingenol and 5-O-(2′E,4′E-decadienoyl)-20-O-acetylingenol, were successfully separated and purified. The established method is rapid and efficient without irritation to operators, providing an extensible and feasible way for the separation and purification of other toxic and skin-irritative compounds. 相似文献
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泽漆化感作用、化学成分及农用生物活性的相关性 总被引:1,自引:0,他引:1
分析了泽漆的化感作用、全草化学成分及农用生物活性的相关性。泽漆根、茎、叶和果实具有不同的化感化用。泽漆中萜、黄酮、酚酸和甾体等不同结构类型的化合物是其具有杀虫、抑菌和对植物生长调节或除草作用的物质基础。泽漆不同产地环境、采收期、部位及提取方法等影响不同结构类型活性成分的比例,进而影响生物活性。寻找泽漆源农用活性先导化合物或新农药有效成分需注意化合物种类、含量与活性的相关性,应重视微量化合物、不同结构类型先导物的复配及化合物的毒性问题,应加强不同结构类型化合物的作用机制研究。 相似文献
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Ching-Chou Tsai Ya-Huei Chang Chi-Chang Chang Ya-Min Cheng Yu-Che Ou Chan-Chao Chang Chien Yi-Chiang Hsu 《International journal of molecular sciences》2015,16(6):12424-12435
Pogostemon cablin (PC) is a traditional herbal medicine used in the treatment of the common cold, nausea, diarrhea, and even for headaches and fever. However, the mechanisms underlying the anti-proliferative activity of PC in endometrial cancer (EC) cells have yet to be fully elucidated. This study investigated the anticancer effects of an aqueous extract of Pogostemon cablin (PCAE), specifically induced apoptosis in EC (Ishikawa) cells. Proliferation of EC cells following exposure to PCAE was assessed by an MTT assay. DNA content and the induction of cell cycle apoptosis were analyzed by flow cytometry (FACS Calibur). Protein caspase-3 and, -9 as well as AIF were investigated using Western blot. Our results demonstrate growth inhibition of Ishikawa cells by PCAE. Furthermore, caspase-3 activity caused PCAE-treated cell lines to accumulate in apoptosis. Gene expression profiling (GEP) results further suggest that, in addition to its known effects with regard to EC prevention, PCAE may also exert antitumor activity on established EC cells. Many previous studies have identified the chemo-preventive effects of natural plant materials and the potential role of these materials in chemotherapy. This current study used human EC Ishikawa cells to investigate the anti-tumor effects of PCAE in EC cells. Our results demonstrate that PCAE inhibits the growth of cancer cells and induces apoptosis, which suggests the potential applicability of PCAE as an antitumor agent. 相似文献
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