共查询到18条相似文献,搜索用时 125 毫秒
1.
2.
蜂王浆是青年工蜂咽下腺和上颚腺分泌的乳白色、淡黄色或浅橙色浆状物质,脂肪酸是蜂王浆的主要活性成分之一,主要包括反式-10-羟基-2-癸烯酸、10-羟基癸酸、3,10-二羟基-癸酸、8-羟基辛酸、癸二酸等,具有抗炎、抗菌和抗肿瘤等多种生物活性。在国家标准中,蜂王浆脂肪酸是评价蜂王浆质量的重要指标,因此脂肪酸的检测对蜂王浆的质量控制具有十分重要的作用。本文综述了目前蜂王浆脂肪酸的检测方法,简要介绍了检测方法的基本原理,同时对不同检测技术和应用特点进行比较,并对今后检测发展的趋势进行了探讨,为进一步开发高效的脂肪酸检测方法及蜂王浆的质量控制提供一定的理论参考。 相似文献
3.
4.
5.
6.
7.
8.
9.
10.
前段时间,香港传媒报道说,有人因服用蜂王浆制品(具体说是XX蜂王精)而导致过敏反应。报道播出后,部分消费者以为吃了蜂王浆就会导致过敏反应,因此,不少市民(特别是正在食用或准备食用蜂王浆的)表示担忧,并向我们咨询。作为蜜蜂产品的专门研究机构,有必要向市民对蜂王浆作些客观公正的介绍。 蜂王浆,是年轻工蜂分泌的“奶”,又称蜂乳。主要成分是蛋白质、氨基酸、维生素、糖类、微量元素、生物活性物质(如超氧物歧化酶(SOD)、胰岛素多肽)、特殊的不饱和脂肪酸——10羟基癸烯酸等。由于蜂王浆被 相似文献
11.
为探明蜂王浆中的植物甾醇组成,蜂王浆样品经冻干、萃取得到王浆脂类物质,再经过皂化、衍生后使用HP-5MS 柱进行分离,采用GC-MS 对蜂王浆中甾醇类物质进行鉴定,并用GC-FID 内标法进行定量分析。结果表明:蜂王浆中除含有少量胆固醇外,还含有25- 羟基-24- 甲基胆甾醇、菜油甾醇、豆甾醇、β - 谷甾醇、Δ5-燕麦甾醇、Δ7- 燕麦甾醇等植物甾醇;蜂王浆中总甾醇含量在51mg/100g 鲜王浆以上。其中,25- 羟基-24- 甲基胆甾醇、菜油甾醇、β- 谷甾醇和Δ5- 燕麦甾醇含量较高,分别占总甾醇含量的36.79%、6.30%、19.75% 和27.99%。 相似文献
12.
Royal jelly is one of the most important products of honeybees. Given its role in development of bee brood into fertile individuals of the royal caste it is also used in health products for human consumption. Royal jelly spoils and loses its health-promoting properties depending on storage duration and conditions. To ensure product quality before selling, it is therefore necessary to assess royal jelly freshness. Many indexes of freshness have been suggested, but they all lack reliability or require complex and time-consuming analyses. Here we describe a method to detect royal jelly freshness based on a chromogenic reaction between royal jelly and HCl. We demonstrate that analyses based on color parameters allow for the discrimination of royal jelly samples based on the duration of their storage. Color parameters of royal jelly stored at -18 and 4 °C for 28 d remained comparable to that of fresh samples, which supports the reliability of the method. The method of freshness determination described is practical, cheap, and fast and can thus be used in real-time when trading royal jelly. PRACTICAL APPLICATION: The method developed can be used to assess royal jelly freshness. It is practical, cheap, and fast and can thus be used in real-time when trading royal jelly. 相似文献
13.
为探究不同花期蜂王浆品质和抗氧化活性的差异,以同产地同蜂种同饲养条件下所生产的蜂王浆作为研究对象,分析不同花期蜂王浆的10-羟基-2-癸烯酸(10-HDA)、总蛋白、水分、总酚酸的含量变化,同时采用DPPH法和FRAP法分析比较其体外抗氧化能力。结果表明,不同花期之间蜂王浆的10-HDA含量存在显著差异(P<0.05),葡萄蜂王浆10-HDA含量最高达到了2.20%。总蛋白含量在13.58%~15.26%之间。蜂王浆的水分含量均≤67.5%,达到国标(GB 9697-2008)关于优等品蜂王浆水分含量的要求。与其他花期蜂王浆相比,荆条花期的总酚酸含量、DPPH自由基清除能力和总抗氧化能力均为最优,且不同花期蜂王浆之间的DPPH自由基清除率存在显著差异(P<0.05)。相关性分析表明,DPPH自由基清除能力和总酚酸以及总抗氧化能力之间均存在极显著正相关性(P<0.01)。 相似文献
14.
Analysis of chloramphenicol in honey and royal jelly by LC/MS/MS 总被引:1,自引:0,他引:1
Ishii R Horie M Murayama M Maitani T 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2006,47(2):58-65
A sensitive and selective method using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was developed for the determination of chloramphenicol (CAP) in honey and royal jelly. Mass spectral acquisition was performed in the negative mode by applying multiple reaction monitoring. In LC separation, Mightyl RP-18GP and 10 mmol/L ammonium acetate-acetonitrile were used as the column and mobile phase, respectively. CAP in honey samples was diluted with water, while CAP in royal jelly was extracted with 1% metaphosphoric acid-methanol (4 : 6). The solutions were cleaned up with an Oasis HLB cartridge. The quantification limits of CAP in honey and royal jelly were 0.3 ng/g and 1.5 ng/g, respectively. The recoveries of CAP from both honey and royal jelly at the quantification limits were over 92%. Twenty honey products and seven royal jelly products were analyzed by the developed method. CAP was detected in one honey product at 0.6 ng/g and in six royal jelly products at the level of 1.5-17.8 ng/g. These results show that the developed method has satisfactory sensitivity selectivity and is useful for the determination of CAP residues in honey and royal jelly. 相似文献
15.
目的:研究蜂王浆蛋白的最佳酶解工艺条件,并分析所制备酶解肽的氨基酸组成、分子量分布、降血糖及抗氧化活性。方法:以蜂王浆为原料,采用碱提酸沉法提取蜂王浆粗蛋白,以α-葡萄糖苷酶抑制率和ABTS自由基清除率为评价指标,筛选出酶解蜂王浆蛋白制备降血糖及抗氧化活性肽的最佳蛋白酶,并研究蜂王浆酶解肽的体外降血糖和抗氧化活性。结果:蜂王浆降血糖及抗氧化活性肽的最佳制备工艺为:以酸性蛋白酶为酶制剂,酶添加量为6000 U/g,酶解温度为43℃,酶解pH为4.0,酶解时间为4 h,料液比为1:10。在上述条件下,制备的蜂王浆蛋白肽的氨基酸总量为82.19%,相对分子质量集中在1000 Da以下。蜂王浆蛋白肽对α-葡萄糖苷酶的半抑制浓度(IC50)为6.94 mg/mL,清除ABTS自由基、羟自由基、1,1-二苯基-2-三硝基苯肼(DPPH)自由基及超氧阴离子自由基的半抑制浓度(IC50)分别为14.18、0.45、11.02和18.38 mg/mL。试验结果表明,蜂王浆蛋白肽具有一定的降血糖和抗氧化活性,可为蜂王浆高值化利用及活性肽产品开发提供理论依据。 相似文献
16.
目的 了解掌握蜂王浆及其制品中的主要营养成分质量情况,以确保蜂王浆在进出口贸易时得到相应的法律保障。方法 样品经乙醇提取,溶出10-羟基-α-癸烯酸(以下简称10-HDA),并沉淀蛋白质,加内标后用乙醇定容,经离心或放置过夜,上清液过滤,HPLC测定。结果 有代表性的进出口的蜂王浆及蜂王浆胶襄样品均含有一定量10-HDA。结论 蜂王浆含果糖、氨基酸、多种酶、乙酰胆碱及丰富的维生素、芸香甙、促进性腺样物质和抗菌素类物质等,而其中10-HAD由于具有一定稳定性,因此是检验蜂王浆营养品质的主要指标。 相似文献
17.
M. Viuda‐Martos Y. Ruiz‐Navajas J. Fernández‐López J.A. Pérez‐Álvarez 《Journal of food science》2008,73(9):R117-R124
ABSTRACT: Honey, propolis, and royal jelly, products originating in the beehive, are attractive ingredients for healthy foods. Honey has been used since ancient times as part of traditional medicine. Several aspects of this use indicate that it also has functions such as antibacterial, antioxidant, antitumor, anti‐inflamatory, antibrowning, and antiviral. Propolis is a resinous substance produced by honeybees. This substance has been used in folk medicine since ancient times, due to its many biological properties to possess, such as antitumor, antioxidant, antimicrobial, anti‐inflammatory, and immunomodulatory effects, among others. Royal jelly has been demonstrated to possess numerous functional properties such as antibacterial activity, anti‐inflammatory activity, vasodilative and hypotensive activities, disinfectant action, antioxidant activity, antihypercholesterolemic activity, and antitumor activity. Biological activities of honey, propolis, and royal jelly are mainly attributed to the phenolic compounds such as flavonoids. Flavonoids have been reported to exhibit a wide range of biological activities, including antibacterial, antiviral, anti‐inflammatory, antiallergic, and vasodilatory actions. In addition, flavonoids inhibit lipid peroxidation, platelet aggregation, capillary permeability and fragility, and the activity of enzyme systems including cyclo‐oxygenase and lipoxygenase. 相似文献
18.
蜂王浆经体积分数95%乙醇提取、MCI-Gel、ODS等反相色谱柱分离,得到单磷酸腺苷(AMP)和7种脂肪酸。采用1,1-二苯基苦基苯肼自由基(DPPH.)清除率和总抗氧化能力试剂盒(T-AOC)等方法探索蜂王浆醇溶性物质的抗氧化能力,结果表明:蜂王浆醇提后抗氧化活性提高,分离得到的AMP和3,10-二羟基癸酸(3,10-DDA)的抗氧化性相对较强,且清除DPPH.的IC50值分别为6.274 mg/mL和9.153 mg/mL,是蜂王浆中的抗氧化活性物质。 相似文献