共查询到18条相似文献,搜索用时 833 毫秒
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转谷氨酰胺酶的性质、制备及在食品加工中的应用 总被引:8,自引:0,他引:8
转谷氨酰胺酶是催化蛋白质分子之间交联的一种酶,对蛋白质的成胶能力、热稳定性、持水能力等功能特性有独特的改善作用。目前从微生物Streptoverticillium spp.中分离转谷氨酰胺酶和在食品工业中的实际应用都已经实现,因为从微生物制备可以实现大规模的工业化生产,成本低廉,为转谷氨酰胺酶在工业上的应用奠定了基础。现在转谷氨酰胺酶广泛地应用于海洋食品、面条/面团、奶制品、烘焙食品等食品加工领域,通过温和的酶反应可以明显地改善食品的硬度、弹性、热稳定性和持水能力。主要讨论了转谷氨酰胺酶的性质、分离和在食品加工中的应用。 相似文献
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转谷氨酰胺酶对乳蛋白质的改性作用 总被引:15,自引:3,他引:15
转谷氨酰胺酶是催化酰基转移反应的一种酶,可使蛋白质或多肽之间发生共价交联反应。本文介绍了转谷氨酰胺酶对牛乳蛋白质凝胶特性、乳化性、热稳定性、成膜性等特性的改性作用及其在乳品加工中的应用。 相似文献
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The effects of transglutaminase on the functional properties of the myofibrillar protein concentrate obtained from beef heart 总被引:1,自引:0,他引:1
The aim of the present study was to evaluate the effect of bacterial transglutaminase on the functional properties of the myofibrillar protein concentrate from beef heart. The degrees of hydration and aggregation and emulsifying properties were studied. The degree of polymerization of the myofibrillar proteins depended on the enzyme concentration and setting time; the best results in terms of functional properties were obtained with 0.3 g transglutaminase/100 g protein with 60 min setting at 35 °C. This investigation confirms that transglutaminase may be used for the production of myofibrillar protein aggregates with enhanced functional properties. 相似文献
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The enzyme microbial transglutaminase (MTGase) (EC 2.3.2.13) catalyzes acyl transfer reactions, deamidation, and inter and intramolecular crosslinks between amino acid residues of glutamine and lysine. Its application in foods alters the technological properties of food proteins, such as their emulsifying capacity, gelation, viscosity, water holding capacity, and foam formation and stability. The commercial application of the enzyme in Brazil is limited almost entirely to the restructured meat industry and dairy beverages, but several studies have demonstrated its potential for commercial application in vegetable products such as soy, which is little exploited in the market. The application of this enzyme to vegetable derivatives allows for the development of new products or improvement of the functional properties of traditional foods. The objective of this article is to offer a comprehensive overview of the general characteristics and function of the enzyme microbial transglutaminase, with emphasis on its application in foods. 相似文献
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微生物谷氨酰胺转胺酶对乳清蛋白的改性 总被引:1,自引:0,他引:1
介绍了该酶的来源、性质及催化反应机理。由于乳清蛋白应用于食品加工中时,其理化功能尚不突出,所以通过谷氨酰胺转胺酶对乳清蛋白的改性,可以加强乳清蛋白的功能性质,从而合理利用资源,开发新产品,扩大其在食品中的应用。 相似文献
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S. Bin Md Yasir K.H. Sutton M.P. Newberry N.R. Andrews J.A. Gerrard 《Food chemistry》2007,104(4):1491-1501
The enzyme transglutaminase was investigated for its cross-linking effect on the soy proteins of tofu. In vitro incubations confirmed that soy proteins are excellent substrates for transglutaminase, especially when denatured. The macroscopic effects resulting from the addition of transglutaminase were compared to changes at the microstructural and molecular level. Treatment produced a firmer tofu, with a significantly increased fracture force. Examination by SEM showed a change in the matrix structure, with transglutaminase resulting in a finer-stranded, uniform network that accounted for the increase in fracture force. At the molecular level, little, if any, cross-linking occurred within the tofu matrix in situ. This suggests that the change in functional properties afforded by addition of transglutaminase to tofu is due to a side reaction of the enzyme, for example hydrolysis of glutamine residues, rather than its cross-linking activity. These ideas are further explored in the accompanying paper. 相似文献
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