The rate of metmyoglobin formation in beef, pork, and turkey meat as influenced by pH, sodium chloride, and sodium tripolyphosphate

This study investigated the effects of pH (5·5 to 7·0), sodium chloride concentration (0·0-3·0%), and sodium tripolyphosphate concentration (0·0 and 0·5%) on the rate of metmyoglobin formation in ground beef, pork and turkey meat during refrigerated storage. Increasing the sodium chloride concentration produced a progressive increase in the rate of metmyoglobin formation in ground beef. Increasing the pH between pH 5·5 and 6·5 had no effect on the rate of metmyoglobin formation with ground beef and turkey meat, but produced a marked decrease between pH 6·5 and 7·0. In contrast pH had no consistent effect on the rate of metmyoglobin formation of ground pork and the rate of formation remained low at all pH levels. When ground beef contained 0·5% sodium tripolyphosphate, the effect of pH was reversed and the rate of metmyoglobin formation was lowest at pH 5·5, increased as the pH increased to 6·5 and then plateaued.     

Relative Viscosity of Proteins Extracted from Frozen, Thawed Turkey Muscle with the Sodium Salts of Nitrite, Chloride, and Tripolyphosphate

Turkey breast muscle batters were prepared with sodium chloride, sodium nitrite, and sodium tripolyphosphate. Batter pH and centrifuged batter sediment weight were measured, extracted protein amounts were measured by the biuret method, and relative viscosity of the extract was determined by capillary tube viscometry. Water and nitrite extracts had similar pH values, protein concentrations and relative viscosities. The batter with sodium chloride had the lowest pH while the batter with sodium tripolyphosphate had the highest pH. The greatest centrifuged batter sediment weight, greatest protein concentration and most viscous extract were from the batter containing sodium chloride and sodium tripolyphosphate.     

Moisture Retention and Textural Properties of Ground Chicken Meat as Affected by Sodium Tripolyphosphate, Ionic Strength and pH

Batches of ground chicken meat were mixed with 10% solutions containing 0.0–5.0% sodium tripolyphosphate and sufficient NaCl to adjust the ionic strength (IS) of the solutions from <0.7 to 6.0 at pH 6.5 to 9.5 in a 6 × 4 × 7 factorial experimental design. Patties prepared from the mixtures were evaluated for pH, cooking loss, and objective texture characteristics. Most effects of STPP were attributable to the direct effect of STPP on IS. However the ability of the phosphate to alter textural properties was greater at a pH value near the pK_a of the phosphate.     

氯化钠和三聚磷酸钠添加量对蒸煮火腿品质的影响

研究不同氯化钠或三聚磷酸钠添加量对蒸煮火腿保水性、质构特性及颜色等指标的影响。结果表明：随着氯化钠添加量的减少,火腿的蒸煮损失率和二次杀菌失水率逐渐增大,而氯化钠添加量降低至1.25%以下时,火腿的硬度、咀嚼度、弹性、黏聚性及回复性极显著下降（P＜0.01）;添加0.20%～0.50%三聚磷酸钠对于保持火腿良好的保水性具有重要作用,为保持火腿良好的硬度和咀嚼度,其添加量需要控制在0.35%以上。     

Color and Color Stability of Frozen Restructured Beef Steaks: Effect of Sodium Chloride, Tripolyphosphate, Nitrogen Atmosphere, and Processing Procedures

This study investigated the effect of mixing and grinding, tempering, addition of sodium chloride and sodium tripolyphosphate and processing under a nitrogen atmosphere on the color of restructured beef steaks initially and at 1-month intervals for 3 months of frozen storage. Mixing and grinding and manufacture under a nitrogen atmosphere had the greatest detrimental effect on the extent of discoloration initially; this was probably due to low oxygen concentration. Tempering and the addition of sodium chloride without sodium tripolyphosphate produced the most rapid increase in rate of discoloration during frozen storage. Tripolyphosphate partially counteracted the detrimental effect of sodium chloride, however, this effect did not appear to be due to the higher pH produced by the phosphate or the chelating ability of the phosphate. Surface discoloration and overall color were highly correlated with surface metmyoglobin (r= -0.87) and overall met-myoglobin (r= -0.94).     

Inhibition of Clostridium sporogenes PA 3679 and Natural Bacterial Flora of Cooked Vacuum-Packaged Bratwurst by Sodium Acid Pyrophosphate and Sodium Tripolyphosphate With or Without Added Sodium Nitrite

Survival and growth of inoculated Clostridium sporogenes PA3679 and natural aerobic and anaerobic bacterial flora were studied in refrigerated (5°C) and subsequently temperature abused (24–25°C), cooked, vacuum-packaged bratwurst containing 0.5% sodium acid pyrophosphate (SAPP) or sodium tripolyphosphate (STPP) with or without sodium nitrite. Phosphates alone or combined with nitrite did not affect aerobic bacterial counts but resulted in reduced clostridial and anaerobic counts at 5°C. Upon temperature abuse, inhibition of all bacteria by SAPP was significant (P<0.05) for up to 48 hr and greatly enhanced by 100 ppm but not by 50 ppm sodium nitrite, whereas STPP lost its antimicrobial properties after 24 hr. Soluble orthophosphate levels had a positive correlation with bacterial inhibition in SAPP-treated bratwurst.     

Effects of L- or D-lactate-enhancement on the internal cooked colour development and biochemical characteristics of beef steaks in high-oxygen modified atmosphere

The effects of L- or D-lactate on internal cooked colour development of steaks packaged in high-oxygen (80% O₂/20% CO₂) modified atmosphere packaging (MAP) was investigated. Ten USDA Select beef strip loins were divided individually into 4 equal-width sections, and one of four treatments (control, 0.3% sodium tripolyphosphate, 2.5% L-lactate + 0.3% sodium tripolyphosphate, and 2.5% D-lactate + 0.3% sodium tripolyphosphate) was assigned randomly to the loin sections. Loin sections were injected to approximately 10% of their raw weight. Steaks packaged in high-oxygen MAP were stored in the dark at 1 °C for 10 days. Instrumental internal colour of raw and cooked steaks (70 °C), total reducing activity (TRA), NADH concentration, and percent myoglobin denaturation (PMD) were measured. Cooked steaks enhanced with 2.5% L-lactate/phosphate maintained higher a*/b* ratios, lower hue values, higher TRA and NADH concentration, and lower PMD than the control and D-lactate-injected steaks, whereas enhancement with 2.5% D-lactate did not affect cooked colour, TRA, NADH, or PMD. Thus, inclusion of an L-lactate/alkaline phosphate blend increased the reducing activity of muscle tissues by replenishing NADH and subsequently decreased the thermal denaturation of myoglobin by maintaining the reduced state of myoglobin in the high-oxygen package.     

Inhibition of Moraxella-Acinetobacter Cells by Sodium Phosphates and Sodium Chloride

The effect of sodium phosphates with and without NaCl, on the ability of unstressed and heat-stressed Moraxella-Acinetobacter (M-A) isolate number 7 cells to form colonies in plate count agar (PCA) was determined. The effectiveness, in order of decreasing ability, of phosphates to inhibit colony formation of unstressed M-A cells was as follows: sodium tripolyphosphate (Na₅P₃O₁₀; STPP), sodium pyrophosphate (Na₄P₂O₇; SPP), and sodium orthophosphate (Na₃PO₄; SP). Filter sterilized STPP was more inhibitory than when heated and inhibition was not related to pH. Certain combinations of NaCl and STPP were additive whereas combinations of NaCl and SPP exhibited a synergistic effect. Heat-stressed M-A cells were more sensitive to levels of NaCl (0.8%) than to levels of STPP (0.1%) or SPP (0.12%); all shown to have no effect on the number of colonies formed in PCA by unstressed cells.     

Effects of Selected Inorganic Phosphates, Phosphate Levels and Reduced Sodium Chloride Levels on Protein Solubility, Stability and pH of Meat Emulsions

The effects were determined of four selected inorganic phosphates at two levels combined with two reduced levels of sodium chloride on the stability, pH, solubilized protein and water-holding capacity of raw meat emulsions. Tetrasodium and tetrapotassium pyrophosphates resulted in higher raw pH, greater protein solubility and improved emulsion stability and water-holding capacity than sodium or potassium tripolyphosphate. Increasing the sodium chloride level from 0.75% to 1.50% decreased the relative effects of phosphate type and level on emulsion stability. The effect of doubling phosphate level on emulsion stability was much less than the effect of doubling the sodium chloride level; however, doubling phosphate level was more effective in increasing pH and soluble protein level of raw emulsions.     

Effects of Salt, Tripolyphosphate and Sodium Alginate on the Texture and Flavor of Fish Patties Prepared from Minced Sheepshead

An attempt was made to develop an acceptable fish patty using minced sheepshead flesh. Deboned and washed sheepshead flesh was mixed with varying proportions of sodium chloride (NaCl), sodium tripolyphosphate (TPP) and sodium alginate (NaAlg). The texture quality of cooked patties was determined by measuring breaking force and sensory acceptability. A regression analysis, performed to measure the effects of NaCl, TPP, and NaAlg as well as to try to determine the optimum combination of ingredients that produced the most acceptable product, showed that all texture responses were significantly influenced by the TPP level (α < 0.05). The breaking force and sensory firmness were strongly influenced by the NaAlg concentration (α < 0.01) which also influenced texture-preference (α < 0.05).     

Evaluation of 0.1% Ammonium Hydroxide to Replace Sodium Tripolyphosphate in Fresh Meat Injection Brines

ABSTRACT:  Paired USDA Select beef strip loins ( n  = 10), aged 2 d, were injected with either an alkaline-based (3.6% sodium chloride, 1% Herbalox seasoning, adjusted to pH 10 with ammonium hydroxide [approximately 0.1%, FFC grade]) or a phosphate-based (3.6% sodium chloride, 1% Herbalox seasoning, 4.5% sodium tripolyphosphate) brine. Steaks were evaluated for 19 d. Overall, phosphate-injected steaks performed better than alkaline-injected steaks with respect to cook yield, water holding capacity, lipid oxidation, color stability, tenderness, and juiciness. Phosphate-injected steaks also had less purge than alkaline-injected steaks, as confirmed by composition analysis. Phosphate-injected steaks were higher in moisture and ash content, and were nearly 2% lower in protein content. Alkaline-injected steaks had significantly lower aerobic (approximately 1 log lower) and anaerobic (approximately 2 log lower) plate counts. Final meat pH probably contributed to the differences observed between treatments. The final pH of phosphate-injected steak was 5.99 while that of alkaline-injected steak was 5.73. Further research should be conducted to determine the concentration of ammonium hydroxide needed in the alkaline-based brine to increase the final meat pH to similar levels found in the phosphate-injected steaks.     

Effect of Turkey Meat, Phosphate, Sodium Lactate, Carrageenan, and Konjac on Residual Nitrite in Cured Meats

ABSTRACT: Wieners were chosen as the model system. They were made with mechanically separated turkey meat (MST) and retained more residual nitrite than other such sausages (p < 0.01). Use of sodium tripolyphosphate resulted in a slightly higher residual nitrite in the finished product (p < 0.05). Wieners containing both MST and sodium tripolyphosphate, or either MST or sodium tripolyphosphate showed higher pH values than the control group. A relationship between product pH and residual nitrite level was observed. Wieners retained more residual nitrite at higher pH. Addition of sodium lactate, carrageenan, and konjac to the wiener formulation did not influence the residual nitrite level in the finished product.     

Influence of sodium chloride and sodium tripolyphosphate on the quality of UK-style grillsteaks: relationship to freezing point depression

Grillsteaks were prepared from lean beef flaked at –4°C and subsequently mixed at nominal temperatures of either –3°C or +1°C with each combination of five sodium chloride levels (0, 0.5, 1.0, 2.0 and 4.0%) and three levels of sodium tripolyphosphate (Na5P3O10) (0, 0.25 and 0.50%).
Sodium chloride was more effective than sodium tripolyphosphate in lowering the initial freezing point (ifp) of meat, as measured by differential scanning calorimetry which also showed that the freezing point depression was proportional to the solute concentration.
Cooking losses generally were lower with higher levels of sodium chloride and sodium tripolyphosphate, and when the meat was mixed at +1°C. It is argued that localized salt concentrations can influence product quality, as can the relative proportions of ice and water when meat is processed below the ifp.     

Tenderness and Sodium Content of Pectoralis superficialis from Broilers Chilled in Potassium or Sodium Chloride Ice Slush

Cooked light meat (Pectoralis superficialis) from broiler carcasses chilled (4 hr, with agitation) in 5% (w/w) sodium chloride (NaCl), 5% (w/w) potassium chloride (KCl), or ice slush was evaluated for moisture content, tenderness (shear force), chloride ion (Cl) concentration, and sodium ion (Na) concentration. Water uptake of the carcasses during chilling was determined. Chilling in either salt solution increased cooked meat moisture, increased chloride ion concentration, and decreased shear force values. Sodium level and water uptake were increased by NaCl brine ice-slush chilling. Samples chilled in NaCl had higher chloride concentrations and lower shear force than samples chilled in KCl.     

INFLUENCE OF THE INTRINSIC PROPERTIES OF FOOD ON THERMAL INACTTVATION OF SPORES OF NONPROTEOLYTIC CLOSTRIDIUM BOTULINUM: DEVELOPMENT OF A PREDICTIVE MODEL1

The effects and interactions of heating temperature (70–90C), pH (5–6.5), sodium chloride (0–3%), and sodium pyrophosphate (0–0.3%) on the heat resistance of a six strain mixture of spores of nonproteolytic Clostridium botulinum type B and type E in turkey were examined. Thermal death times were determined in submerged vials heated using a water bath. Heated spores were recovered on Reinforced Clostridial Medium (RCM) supplemented with lysozyme (10 μg/ml). Decimal reduction times (D-values) were calculated by fitting a survival model to the data with a curve fitting program. The D-values were analyzed by second order response surface regression for temperature, pH, salt (sodium chloride) and sodium pyrophosphate levels. The four variables interacted to effect the inactivation of spores. Confidence intervals (95%) predicted heat resistance of spores in turkey. The data suggest that the effect of reduced pH in increasing the inactivation was more pronounced at high temperatures and may provide an adequate degree of protection from nonproteolytic C. botulinum spores in minimally processed foods, particularly if employed in conjunction with combinations of salt and sodium pyrophosphate.     

Kappa-Carrageenan, Sodium Chloride and Temperature Affect Yield and Texture of Structured Beef Rolls

Structured lean beef rolls (4–5% fat), formulated with 33% added water, 1% sodium chloride and 0.35% sodium tripolyphosphate and cooked to 63°, 73° and 83°, had low cook yields and poor texture and bind. Kappa-carrageen an (KC) added at 0.5–1% and NaCl at 2–3% increased cook yield and improved textural properties (bind, force to fracture, hardness). Rolls with 1.0% KC and 3% NaCl had the highest cook yield (154% meat weight basis) and the highest values for force to fracture and hardness. Cook yield decreased and hardness increased with increased cooking temperature. Effects of KC on yield and texture were most pronounced at the lowest NaCl level (1%) and the highest temperature (83°). KC also reduced purge of vacuum-packaged slices during refrigerated storage.     

Synergistic effect of Sodium Chloride,temperature and pH on growth of a cocktail of spoilage yeasts: a research note

The effect of sodium chloride (0·5–10% w/v), pH (2·6–6·3) and temperature (1–22°C) were studied on the growth of a cocktail of food spoilage yeasts. The length of the lag phase and the time taken to reach the level of 10⁶cfu ml⁻¹were calculated for each set of conditions. It was found that the lag phase constituted as little as 21% of the total time to reach 10⁶cfu ml⁻¹when the yeasts were grown in favourable conditions and as much as 62% of the total time when more extreme conditions were used. It was concluded that the lag phase was the most important factor affecting the spoilage potential of chilled foods with low pH and high salt values. The single most effective factor in reducing the growth rate of yeasts was temperature. The lag phase was 15, 38, 270, 630 and 875 h when the temperature was 15, 8, 4, 2 and 1°C respectively. At any single temperature, there appeared to be a synergistic effect of NaCl and pH and under the most extreme conditions tested (1°C; pH 5·8; 6% NaCl), the lag phase was over 1000 h. These data have implications for the spoilage potential of high salt, reduced pH foods stored at chill temperatures.     

Microbiological and Sensory Changes in Minced Beef Treated with Potassium Lactate and Sodium Diacetate during Refrigerated Storage

The effect of potassium lactate and sodium diacetate on the microbiological changes and sensory properties of vacuum-packaged minced beef was investigated. The meat samples both with a preservative (in the amounts 0.65% and 1.3%) and without were stored at temperatures of 0–1°C and 5–6°C. The influence of storage time on changes in total bacteria count (TBC), lactic acid bacteria (LAB), Brochothrix thermosphacta, and the microbes of the Enterobacteriaceae family was investigated, as well as changes in pH and sensory quality. It was found that the addition of the preservative to the minced meat caused a significant extension (p < 0.05) of the lag phase and an inhibition of microbial growth rate, depending on temperature, storage time, and its concentration. The antibacterial effect was significantly higher (p < 0.05) at a temperature of 0–1°C than at 5–6°C and most susceptible to it were the bacteria belonging to the Enterobacteriaceae. The study results showed that the minced beef containing the preservative which had been vacuum stored at 0–1°C, presented a better sensory quality and had a shelf-life of about 6 days longer, in relation to the quality and shelf-life of the control samples. For each of the refrigeration storage temperatures however, there was no statistically significant change (p < 0.05) in the pH for the various storage periods and preservative quantities present.     

Calcium chloride and tricalcium phosphate effects on the pink color defect in cooked ground and intact turkey breast

Calcium chloride (250, 500 ppm) was examined for its ability to reduce the pink color defect induced by sodium nitrite (10 ppm) and nicotinamide (1.0%) in cooked ground turkey in the presence and absence of sodium tripolyphosphate (0.25, 0.5%) and sodium citrate (0.5, 1.0%). The ability of tricalcium phosphate (0.1–0.5%) to reduce pink cooked color also was evaluated in ground turkey and both calcium chloride and tricalcium phosphate were tested for their effects on pink cooked color in whole breast muscle. The combination of calcium chloride and sodium tripolyphosphate, not calcium chloride alone, was necessary for a reduction in pink cooked color induced by nicotinamide. Subsequently, in the presence of phosphate, both calcium chloride and sodium citrate reduced pink cooked color and were most effective in combination. Tricalcium phosphate also was capable of reducing pink cooked color in ground turkey, however substituting tricalcium phosphate for sodium tripolyphosphate resulted in lower pH and cooking yields. Neither calcium chloride nor tricalcium phosphate was capable of reducing pink cooked color in whole turkey breast. Currently, a combination of sodium tripolyphosphate, calcium chloride, and sodium citrate represents the most suitable means for reducing or preventing the pink color defect in uncured ground turkey.     

Protein Extraction from Frozen, Thawed Turkey Muscle with Sodium Nitrite, Sodium Chloride, and Selected Sodium Phosphate Salts

Turkey muscle batters were prepared with the sodium salts of nitrite, chloride and selected phosphate salts. Amounts of extracted protein were measured by the biuret method and separated by SDS-gel elctrophoresis and high pressure liquid chromatography (HPLC). For breast muscle, three proteins in the nitrite extracts had higher molecular weights than those in water extracts as determined by SDS-gel electrophoresis. Sodium chloride and sodium tripolyphosphate increased centrifuged batter sediment weight, increased the concentration of extracted protein, increased the number of electrophoretically separated higher molecular weight protein bands and increased HPLC peak areas of extracts from muscle batters.