共查询到20条相似文献,搜索用时 31 毫秒
1.
Margit Winkler Dr. Thomas Moraux Hesham A. Khairy Roderick H. Scott Dr. Alexandra M. Z. Slawin Prof. David O'Hagan Prof. 《Chembiochem : a European journal of chemical biology》2009,10(5):823-828
Spicing it up with fluorine : Enantiomers of α‐fluorinated capsaicin 2 , have been prepared by organocatalytic electrophilic fluorination and have been used as probes for the binding conformation of capsaicin to the TRPV1 pain receptor. No enantiomeric bias is observed, thus suggesting an extended binding conformation along the molecular axis.
2.
Raffaella Caglio Dr. Francesca Valetti Dr. Patrizia Caposio Dr. Giorgio Gribaudo Prof. Dr. Enrica Pessione Prof. Dr. Carlo Giunta Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(6):1015-1024
Choosing chloro : By reshaping the catalytic pocket of a catechol 1,2‐dioxygenase through a structural route alternative to evolution, novel engineered chlorocatechol dioxygenase‐like enzymes were obtained. Variants show an inversion of specificity with a preference for 4‐chlorocatechol and activity on the rarely recognised substrate 4,5‐dichlorocatechol.
3.
《Journal of the American Ceramic Society》2016,99(6):i-i
Transmission electron microscopy image of crack trajectories in Vicker's indented LaB6 (matrix)‐ ZrB2 (fibers) directionally solidified eutectic. (Image credit: Hongqi Deng and Elizabeth C. Dickey). See feature article this issue.
4.
Carlo Morasso Dr. Tommaso Bellini Prof. Dr. Daniela Monti Dr. Mattia Bassi Davide Prosperi Dr. Sergio Riva Dr. 《Chembiochem : a European journal of chemical biology》2009,10(4):639-644
Stretch out : An elastic light‐scattering‐based method that makes use of phantom nanoparticles as a substrate organizer (see illustration) allowed the quantitative evaluation of the molecular recognition events between a series of inactivated mutants of phospholipase D and lysophosphatidylcholine. The results highlight the remarkable effects on binding capability caused by single amino acid substitutions.
5.
Gertjan Veldhuis Dr. Ine Segers‐Nolten Dr. Eva Ferlemann Vinod Subramaniam Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(3):436-439
SDS‐concentration‐dependent α‐synuclein structure : Upon interaction with SDS, αSyn folds into a structure with two antiparallel α‐helices. We show from single‐molecule FRET that αSynn adopts this conformation in an all‐or‐none fashion below the SDS critical micelle concentration. Population of the folded species is directly coupled to an increase in α‐helix content; this suggests that the entire N terminus is involved in the transaction.
6.
Steffen Brenzel Menekse Cebi Dr. Philipp Reiß Dr. Ulrich Koert Prof. Dr. Henning D. Mootz Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(6):983-986
It's raining, it's porin : Fragment ligation of OmpF ion channels was achieved by using the split Psp‐GBD Pol intein; this allowed reconstitution of active trimeric porin. In combination with cysteine modification at an internal position, the porin's conductance properties were altered.
7.
Guojie Wang Dr. Georgii V. Bobkov Sergey N. Mikhailov Prof. Guy Schepers Arthur Van Aerschot Prof. Jef Rozenski Prof. Mark Van der Auweraer Prof. Piet Herdewijn Prof. Steven De Feyter Prof. 《Chembiochem : a European journal of chemical biology》2009,10(7):1175-1185
Powerful pyrene probes : Two kinds of pyrene‐labeled oligonucleotides (HNA‐ and RNA‐skeleton probes) were explored. The enhanced fluorescence intensity in the monomer region and the disappearance of aggregate/excimer emission in duplexes has been successfully used to detect the hybridization of oligonucleotides.
8.
Madan Kumar Kharel Dr. Pallab Pahari Dr. Hui Lian Jürgen Rohr Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(8):1305-1308
Last at last : The terminal step of the gilvocarcin V (GV) biosynthetic pathway is an unusual lactone formation. Here we show that the enzyme, GilR, dehydrogenates the hemiacetal moiety of pregilvocarcin V to the lactone found in GV by using covalently bound FAD.
9.
J. S. Shane Rountree Dr. Terry D. Butters Dr. Mark R. Wormald Dr. Stephanie D. Boomkamp Raymond A. Dwek Prof. Naoki Asano Prof. Kyoko Ikeda Dr. Emma L. Evinson Dr. Robert J. Nash Dr. George W. J. Fleet Prof. 《ChemMedChem》2009,4(3):378-392
Combating glycolipid storage disorders : LABNAc was prepared in an efficient 11‐step procedure from D ‐lyxonolactone. The enantiomer DABNAc was also prepared from L ‐lyxonolactone. Preliminary cellular studies indicate that these compounds may find utility as chemical chaperones for the treatment of Tay‐Sachs and Sandhoff diseases.
10.
Sampat Ingale Dr. Margreet A. Wolfert Dr. Therese Buskas Dr. Geert‐Jan Boons Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(3):455-463
Synthetic cancer vaccines : A number of fully synthetic vaccine candidates have been designed, chemically synthesized, and immunologically evaluated to establish a strategy to overcome the poor immunogenicity of tumor‐associated carbohydrates and glycopeptides and to determine the importance of Toll‐like receptor (TLR) engagement for antigenic responses against these compounds.
11.
John Yan Shuchi Gupta Dr. David H. Sherman Prof. Dr. Kevin A. Reynolds Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(9):1537-1543
Working together or apart : Separating multimodular PKS enzymes into their respective monomodules by replacing the natural intraprotein linkers (illustrated in red in the figure) with a matched docking domain pair from a heterologous PKS system, leads to only small losses in overall in vivo polyketide product and increased efficiency at utilizing polyketide pathway intermediates to prime the biosynthetic process.
12.
Dirk Schwarzer Dr. 《Chembiochem : a European journal of chemical biology》2009,10(10):1602-1604
A genetic shuttle : The highlighted article, which was recently published by Schultz, Geierstanger and co‐workers, describes a straightforward scheme for enlarging the genetic code of mammalian cells. An orthogonal tRNA/aminoacyl‐tRNA synthetase pair specific for a new amino acid can be evolved in E. coli and subsequently transferred into mammalian cells. The feasibility of this approach was demonstrated by adding a photocaged lysine derivative to the genetic repertoire of a human cell line.
13.
Sylvain Célanire Dr. Maikel Wijtmans Dr. Bernard Christophe Dr. Philippe Collart Iwan de Esch Dr. Donald Dassesse Dr. Christel Delaunoy Frédéric Denonne Dr. Véronique Durieu Edith Gelens Dr. Michel Gillard Dr. Bénédicte Lallemand Yves Lamberty Dr. Florence Lebon Dr. Jean‐Marie Nicolas Dr. Luc Quéré Dr. Erwin Snip Dr. Alain Vanbellinghen Nathalie Van houtvin Valérie Verbois Henk Timmerman Prof. Dr. Patrice Talaga Dr. Rob Leurs Prof. Dr. Laurent Provins Dr. 《ChemMedChem》2009,4(7):1063-1068
H 3 R inverse agonists based on an aminopropoxy‐phenyloxazoline framework constitute highly valuable druglike lead compounds that display efficacy in a mouse model of recognition memory.
14.
Marc‐André Frese Thomas Dierks Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(3):425-427
Oxidation of a specific cysteine residue to Cα‐formylglycine is a novel post‐translational modification that is directed by a short recognition motif commonly found in pro‐ and eukaryotic sulfatases. As recently shown by C. Bertozzi and co‐workers, this system can be employed in protein engineering to equip proteins with genetically encoded aldehyde tags for site‐specific labeling, conjugation and immobilization.
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16.
Sébastien G. Gouin Dr. Adinda Wellens Julie Bouckaert Dr. José Kovensky Prof. 《ChemMedChem》2009,4(5):749-755
Sweet medicine : Multimeric glycoconjugates with valencies ranging from one to four were synthesized by click chemistry. Unprecedented adhesion inhibitions of piliated E. coli to human bladder cells were recorded with the multimers; a tetravalent derivative showed inhibitory concentrations 6000‐ and 64‐fold lower than mannose and heptyl α‐D ‐mannoside, respectively.
17.
Daniel M. Lewallen Dr. David Siler Suri S. Iyer Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(9):1486-1489
Glycan binding : We monitored the binding of synthetic glycans to influenza hemagglutinin by using ELISA and surface plasmon resonance, thereby demonstrating that the glycan's presentation influences binding dramatically. Also, the binding observed in static systems was very different from that in dynamic fluid systems. These studies suggest that binding specificities are dependent on glycan structure, valency, presentation, and assay conditions.
18.
Andrew C. Mercer Dr. Jordan L. Meier Justin W. Torpey Dr. Michael D. Burkart Prof. 《Chembiochem : a European journal of chemical biology》2009,10(6):1091-1100
Insider information : Selective labeling of endogenous proteins within cells has been an elusive goal. Here carrier protein labeling has been optimized for visualization, isolation, and protein sequencing.
19.
Antimycotic agents : Diverse classes of antimycotic drugs have been developed over the past decades with the goal of improving selectivity and efficacy. This review discusses both conventional and novel targets for antifungal agents and the possibility of vaccination in the treatment of invasive fungal infections.
20.
Martijn A. J. Veld Linda Fransson Dr. Anja R. A. Palmans Dr. E. W. Meijer Prof. Dr. Karl Hult Prof. 《Chembiochem : a European journal of chemical biology》2009,10(8):1330-1334
Size matters : Lactones have extensively been studied as monomers in enzymatic polymerization reactions. Large lactones showed an unexpectedly high reactivity in these reactions. A combination of docking and molecular dynamics studies have been used to explain this high reactivity in terms of productive binding due to the transoid nature of the ester bond in these substrates.