Fabrication and characterization of chitosan/poly(vinyl alcohol) electrospun nanofibrous membranes containing silver nanoparticles for antibacterial water filtration

Electrospun nanofibrous membranes (ENMs) were fabricated based on chitosan/poly(vinyl alcohol) (CS/PVA) with a 70/30 mass ratio containing silver nanoparticles (AgNPs) via the electrospinning method. AgNPs were produced on the surface of CS/PVA nanofibers by adding AgNO₃ to a CS/PVA blend solution as a silver rendering component. The presence of AgNPs in the polymer blend solution was detected by UV spectrophotometry. The morphology of nanofibers before and after cross-linking with glutaraldehyde was investigated by the field emission scanning electron microscopy. The formation and size distribution of AgNPs onto the surface of nanofibers were observed by transmission electron microscopy and confirmed by energy dispersing X-ray spectroscopy. As-spun and cross-linked CS/PVA nanofibers revealed a smooth surface with diameters ranging from 58 to 73 nm and 95 to 109 nm, respectively. The effect of AgNP formation on the chemical structure of nanofibers was explored by Fourier transform infrared spectroscopy. Static and dynamic antibacterial filtration efficiencies of CS/PVA ENMs, containing differing amounts of AgNO₃, have been tested against Escherichia coli, a gram negative bacterium. The antibacterial assessment results exhibited a significant increase in both static and dynamic antibacterial filtration efficiencies of the prepared CS/PVA ENMs by addition of AgNO₃ as a bactericidal agent.     

Radiation synthesis and characterization of polyvinyl alcohol/chitosan/silver nanocomposite membranes: antimicrobial and blood compatibility studies

Polyvinyl alcohol/chitosan/silver (PVA/CS/Ag) nanocomposite membranes were synthesized by γ-radiation with promising antimicrobial and biomedical applications. The nanocomposite membranes were prepared by mixing PVA and CS solutions with different copolymer compositions in the presence of silver nitrate (AgNO₃) and glutaraldehyde as cross-linker, followed by in situ reduction with γ-radiation at different doses. The nanocomposite membranes were characterized by ultraviolet spectroscopy (UV), Fourier transform infrared, X-ray diffraction (XRD) and transmission electron microscopy (TEM). UV studies showed a strong peak around λ _max at 430 nm due to surface plasmon resonance of silver nanoparticles formed during irradiation. As the irradiation dose increased from 25 to 75 kGy, the plasmon band is shifted from 430 to 418 nm with high intensity, indicating the formation of smaller particles. TEM investigation showed uniform distribution of silver nanoparticles (AgNPs) in the membranes with mean diameter of 32–19 nm. XRD results confirmed that the mean diameter of AgNPs estimated from the Debye–Scherrer formula was in the range of 27.5–12.8 nm which confirms the TEM results. The PVA/CS/Ag nanocomposite membranes exhibited good antibacterial activity and were found to cause significant reduction in microbial growth. The nanocomposite membranes showed non-thrombogenicity effect and slightly haemolytic potential, suggesting their promising use in biomedical applications.     

Effect of plasma duty cycle on silver nanoparticles loading of cotton fabrics for durable antibacterial properties

A facile method for strongly anchoring silver nanoparticles (AgNPs) onto cotton fabrics was reported. It consists in loading AgNPs onto the cotton fiber preliminary coated with maleic anhydride plasma polymer layer. This results in hydrolyzis and ring opening of anhydride groups followed by electrovalent bonding of silver ions and reduction in NaBH₄. X‐ray photoelectron spectroscopy (XPS), infrared spectroscopy, and scanning electron microscope (SEM) were used to analyze changes in the surface chemical composition and morphology of the plasma modified fibers. The presence of AgNPs was confirmed by UV–Visible spectroscopy and atomic force microscopy (AFM) images. Remarkably, varying plasma duty cycle for plasma polymer deposition allowed tailoring the amount of loaded AgNPs. The highest amount of AgNPs was obtained with the lowest duty cycle values. Qualitative tests showed that silver containing plasma modified cotton displays significant antibacterial activity. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41279.     

MOF-199@GO改性PVDF荷电纳滤膜的制备及其性能

采用原位生长法制备了MOF-199@氧化石墨烯（GO）纳米复合材料,并对聚偏氟乙烯（PVDF）支撑膜进行表面改性,以克服PVDF膜表面疏水性。通过界面聚合反应,制备了基于MOF-199@GO改性PVDF的聚酰胺复合荷电纳滤膜。采用XRD、SEM、TEM、AFM和zeta电位等手段表征了MOF-199@GO复合材料及MOF-199@GO改性PVDF聚酰胺复合纳滤膜的结构及微观形貌,并测试了MOF-199@GO改性PVDF聚酰胺复合纳滤膜的脱盐性能。结果表明：通过MOF-199@GO复合材料对PVDF支撑膜的表面改性,有效克服了PVDF支撑膜的疏水性,实现了表面聚酰胺薄层的均匀连续生长,荷电纳滤膜表面荷负电性能显著增强,其中经MOF-199@GO充分改性的复合荷电纳滤膜表现出优异的脱盐性能,对MgSO₄、Na₂SO₄、NaCl和MgCl₂四种盐的截留率分别达到了93.56%、93.04%、87.48%和87.11%。     

Preparation and properties of MOF-199@GO modified PVDF charged composite nanofiltration membrane

As novel functional materials, metal-organic framework (MOF) and graphene oxide (GO) have received great attentions in recent years. In this work, MOF@GO nanocomposite (MOF-199@GO) is prepared by an in-situ growth method. A novel and highly efficient nanofiltration (NF) membrane can be facilely fabricated via surface decoration of MOF-199@GO onto poly(vinylidene fluoride) (PVDF) substrate before interfacial polymerization of m-phenylenediamine (MPD) and trimesoyl chloride (TMC) in order to overcome the hydrophobicity of PVDF membrane. The structure and morphology of MOF-199@GO and MOF-199@GO modified PVDF polyamide composite membrane are characterized by XRD, SEM, TEM, AFM and zeta potential. MOF-199@GO modified PVDF composite NF membrane which possesses dense and uniform polyamide thin-layer exhibits higher negative surface potential (up to ~37 mV) at pH 9.5. The performance of MOF-199@GO modified PVDF polyamide composite NF membrane has been investigated by determination of pure water flux and salt rejection. The prepared NF membrane MG3 exhibited highly efficient rejection of MgSO₄, Na₂SO₄, NaCl and MgCl₂, which are 93.56%, 93.04%, 87.48% and 87.11%, respectively. This work provides a worthy reference for designing highly efficient NF membranes modified by MOF and relevant materials.     

High performance forward osmosis cellulose acetate (CA) membrane modified by polyvinyl alcohol and polydopamine

Cellulose acetate (CA) is a low cost and readily available material widely used in forward osmosis (FO) membranes. However, the performance of pure CA membranes is not good enough in salt separation and the traditional modification methods are generally multistep and difficult to control. In this paper, we reported high performance cellulose acetate (CA) composite forward osmosis (FO) membranes modified with polyvinyl alcohol (PVA) and polydopamine (PDA). PVA was first cross-linked onto the surface of CA membranes, and then PDA was coated with a rapid deposition method. The membranes were characterized with respect to membrane chemistry (FTIR and XPS), surface properties comprising wettability (by water contact angle), and osmosis performance. The modified membrane coated by PVA and PDA shown better hydrophilicity and exhibited 16.72 LMH osmotic water flux and 0.14 mMH reverse solute flux with DI water as feed solution and 2.0 M NaCl as draw solution and active layer facing the feed solution. This simple and highly effective modification method makes it as an excellent candidate for further exploration for FO.     

Synthesis and characterization of PVA/PES thin film composite nanofiltration membrane modified with TiO2 nanoparticles for better performance and surface properties

Novel polyethersulfone (PES)/poly (vinyl alcohol) (PVA)/titanium dioxide (TiO₂) composite nanofiltration membranes were prepared by dip-coating of PES membrane in PVA and TiO₂ nanoparticles aqueous solution. Glutaraldehyde (GA) was used as a cross-linker for the composite polymer membrane in order to enhance the chemical, thermal as well as mechanical stabilities. TiO₂ nanoparticles with different concentrations (0, 0.05, 0.1, 0.5 wt.%) were coated on the surface of PVA/PES composite membrane. The morphological study was investigated by atomic force microscopy (AFM), scanning surface microscopy (SEM) and along with X-ray diffraction (XRD). In addition, the membranes performances, in terms of permeate flux, ion rejection and swelling factor were also investigated. It was found that the increase in TiO₂ solution concentration can highly affect the surface morphology and filtration performance of coated membranes. The contact angle measurement and XRD studies indicated that the TiO₂ nanoparticles successfully were coated on the surface of PVA/PES composite membranes. However, rougher surface was obtained for membranes by TiO₂ coating. The filtration performance data showed that the 0.1 wt.% TiO₂-modified membrane presents higher performance in terms of flux and NaCl salt rejection. Finally, TiO₂ modified membranes demonstrated the lower degree of swelling.     

Enhancement of pervaporation performance of composite membranes through in situ generation of silver nanoparticles in poly(vinyl alcohol) matrix

Composite membranes were prepared from an aqueous solution of poly(vinyl alcohol) (PVA) and silver sulphate. The silver nanoparticles were generated in situ before crosslinking PVA matrix by reduction of silver ions using sodium borohydride. Physico‐chemical properties of the resulting composite membranes were studied using Fourier transform infrared spectroscopy (FTIR), UV–vis spectroscopy (UV–vis), thermogravimetric analysis (TGA), Wide‐angle X‐ray diffraction (WAXD), scanning electron microscopy (SEM), and universal testing machine (UTM). The UV–vis spectrum shows a single peak at 410 nm due to surface plasmon absorption of silver nanoparticles. This surely specified that silver nanoparticles are generated in PVA matrix. The membranes were under go pervaporation (PV) for separation of water from isopropanol at different temperatures. The results indicated that hydrophilicity and amorphous nature of the membranes were increased with increasing silver nanoparticles in PVA matrix. The swelling and separation performance of the membranes were studied. Both permeation flux and separation factor were increased with increasing silver nanoparticles in PVA matrix. The results showed that the membrane containing 2.5 mass% of Ag salt exhibited excellent PV performance. The values of total flux and flux of water are almost closed to each other, indicating that membranes could be effectively used to break the azeotropic point of water‐isopropanol. The long‐term test was performed at room temperature and ascertained that membranes were durable up to 30 days for the dehydration of IPA. On the basis of the estimate Arrhenius activation energy values, the efficiency of the membranes was discussed. The calculated ΔH_s values are negative for all the membranes, indicating that Longmuir's mode of sorption is predominant. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41248.     

Performance assessment of poly (methacrylic acid)/silver nanoparticles composite as corrosion inhibitor for aluminium in acidic environment

This article reports the preparation of poly (methacrylic acid)/silver nanoparticles composite (PMAA/AgNPs) by in situ mixing of aqueous solutions of PMAA and 1 mM AgNO₃ with natural honey as reducing and stabilizing agent. The prepared PMAA/AgNPs composite was characterized by UV–vis spectroscopy, Fourier transformed infrared, X-ray diffraction and energy dispersive spectroscopy (EDS) while the morphology of the AgNPs in the composite was obtained by transmission electron microscopy (TEM). TEM results revealed that the AgNPs were spherical in shape. The performance of PMAA/AgNPs composite as anticorrosion material for Al in acidic environment was examined by electrochemical, weight loss methods, complemented by surface analysis characterization with scanning electron microscopy (SEM), EDS and water contact angle measurements. Results obtained indicate that PMAA/AgNPs composite is effective corrosion inhibitor for Al in an acid-induced corrosive environment. Inhibition efficiency increased with increase in composite concentration but decreased with rise in temperature. From potentiodynamic polarization results, PMAA/AgNPs composite is found to function as a mixed-type corrosion inhibitor. The adsorption of the composite onto Al surface was found to follow El-Awady et al. adsorption isotherm model. SEM, EDS and water contact angle results confirmed the adsorption of the composite on Al surface.     

PVA/OPD改性膜对微生物燃料电池性能的影响

为提高微生物燃料电池（MFC）的性能，以聚乙烯醇（PVA）为黏合剂，分别以磷钨酸（PWA）和邻苯二胺（OPD）为改性剂，采用溶液浸渍法制备了PVA/PWA和PVA/OPD改性膜并搭载于MFC系统，以SEM、电化学阻抗谱（EIS）、循环伏安法（CV）、吸水率表征了膜的性能，并考察膜改性对MFC产电量和化学需氧量（COD）去除率的影响。结果显示，PVA/PWA和PVA/OPD改性膜均能在一定程度上提升MFC性能，但PVA/OPD改性膜效果更佳。PVA/OPD改性膜的吸水率为14.49%，较常规Nafion 117膜（NF）提高了126.05%。采用PVA/OPD改性膜的MFC在测试周期内的产电量为101.75 J，较采用NF时提高了587.50%；对阿莫西林制药废水的COD去除率为66.2%，较采用NF时提高了48.4%。基于PVA/OPD的膜改性方法对提高MFC的产电性能和废水处理效果有显著作用。     

Zwitterionic‐polymer‐functionalized poly(vinyl alcohol‐co‐ethylene) nanofiber membrane for resistance to the adsorption of bacteria and protein

A zwitterionic poly(vinyl alcohol‐co‐ethylene) (PVA‐co‐PE) nanofiber membrane for resistance to bacteria and protein adsorption was fabricated by the atom transfer radical polymerization of sulfobetaine methacrylate (SBMA). The PVA‐co‐PE nanofiber membrane was first surface‐activated by α‐bromoisobutyryl bromide, and then, zwitterionic SBMA was initiated to polymerize onto the surface of nanofiber membrane. The chemical structures of the functionalized PVA‐co‐PE nanofiber membranes were confirmed by attenuated total reflectance–Fourier transform infrared spectroscopy and X‐ray photoelectron spectroscopy. The morphologies of the PVA‐co‐PE nanofiber membranes were characterized by scanning electron microscopy. The results show that the poly(sulfobetaine methacrylate) (PSBMA) was successfully grafted onto the PVA‐co‐PE nanofiber membrane, and the surface of the nanofiber membrane was more hydrophilic than that of the pristine membrane. Furthermore, the antibacterial adsorption properties and resistance to protein adsorption of the surface were investigated. This indicated that the PSBMA‐functionalized surface possessed good antibacterial adsorption activity and resistance to nonspecific protein adsorption. Therefore, this study afforded a convenient and promising method for preparing a new kind of soft and nonwoven dressing material with antibacterial adsorption and antifouling properties that has potential use in the medical field. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 44169.     

Preparation and characterization of carboxylated multiwalled carbon nanotube/polyamide composite nanofiltration membranes with improved performance

Polyamide thin‐film composite nanofiltration (NF) membranes were prepared via the interfacial polymerization (IP) process of piperazine and 1,3,5‐trimesoyl chloride on the polysulfone/nonwoven fabric ultrafiltration membrane surface. Carboxylated multiwalled carbon nanotubes (cMWNTs) were incorporated into the aqueous phase during the IP process to improve the membrane performance. The composition and morphology of the membrane surface were examined by means of attenuated total reflectance–Fourier transform infrared spectroscopy, scanning electron microscopy–energy dispersive spectrometry, and atomic force microscopy. The effects of the cMWNTs content on the membrane hydrophilicity, separation performance, and antifouling properties were characterized through water contact angle and crossflow filtration measurements. The experimental results show that membrane surface hydrophilicity, water permeability, salt rejection (R ), and antifouling properties all improved. In particular, when the cMWNTs content was 50 ppm, the magnesium sulfate R of the composite NF membrane reached a maximum value of 98.5%; meanwhile, the membrane obtained an obviously enhanced water flux (62.1 L m^?2 h^?1 at 0.7 MPa), which was two times larger than that of the original NF membrane. The modified NF membranes showed enhanced antifouling properties; this was mainly attributed to changes in the microstructures and surface features of the polyamide layer after the addition of the cMWNTs. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45268.     

基于植物质还原的银纳米颗粒的制备及在织物抗菌整理上的应用

纳米银是以纳米技术为基础研制而成的新型抗菌产品,由于量子效应和尺寸效应具有普通银系抗菌剂无法比拟的抗菌效果。本研究以黄芩、丁香、洋浦桃、芳樟4种植物质提取液制备银纳米颗粒,并借助于UV-Vis、TEM以及XRD对产物进行表征,结果表明增加植物质提取液浓度或增大NaOH加入量,均有利于制得粒径较小的银纳米颗粒。SEM图片表明采用浸渍法可将所得纳米银颗粒负载于纯棉织物上,通过考察浸渍时间、温度、浴比对织物上载银量的影响,确定较优的浸渍条件为时间30 h,温度55℃,浴比为1:25。分别考察了银纳米颗粒粒径和植物质种类对所得载银织物抗菌性能的影响,发现负载到织物上的纳米银粒径越小,织物抑菌效果越好;利用本身具有抑菌效果的黄芩、丁香来制备银纳米颗粒,有利于增强所得载银织物的整体抗菌性能;4种植物质中以黄芩制得的载银织物抗菌效果最优,对金黄色葡萄球菌、大肠杆菌等实验菌株均有强烈的抑制作用。     

Modification of regenerated cellulose membrane by impregnation of silver nanocrystal clusters

Regenerated cellulose forms a very important class of basic material with diverse applications because of its hydrophilicity and insolubility in water. Thus, one of the applications of regenerated cellulose is used to fabricate membranes. However, short operational lifetime is one of the disadvantages of the regenerated cellulose. In this research, surface modification of the cellophane membrane was carried out by silver nanoclusters. Silver colloids were formed in situ by chemical and photochemical reduction, and then, silver particles were deposited uniformly onto the surface of the cellophane membrane. The maximum amount of silver deposition was found to be 2.55% by weight in this modification. The modified and unmodified membranes were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray analysis to indicate silver nanocrystalline cluster particles on the modified membrane. SEM images indicate well-dispersed silver particles with an average size of 0.65 μm on the membrane. XRD patterns showed that the size of the silver crystals was 3.9 nm. The surface properties of modified and unmodified membranes were studied by the contact angle. Water absorption, oxidative resistance, salt permeability, and thermal stability were investigated. This study revealed that the modified membrane is more resistant against the oxidative cleavage than the unmodified one moreover, the salt permeability increased after the treatment. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48292.     

Membrane-assisted capacitive deionization: Effect of Poly(vinyl alcohol) cross-linking on the properties of ion exchange membranes

Novel composite electrodes were developed for application in membrane capacitive deionization (MCDI). Activated carbon (AC) was dispersed in a solution of poly(vinyl alcohol) (PVA) mixed with polyacrylic acid (PAA) or poly dimethyl diallyl ammonium chloride (PDMDAAC), and cast onto the surface of an AC-based modified graphite electrode, prepared by phase inversion, to form a composite membrane further cross-linked with glutaraldehyde (GA). The effect of the cross-linking on the chemical structure of the PVA-based membranes was determined by attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy. Cyclic voltammetry was conducted to examine the specific capacitance of the composite electrodes. Desalination experiments were then performed with MCDI unit cells to study the effect of cross-linking on the desalination efficiency. It was proved that after optimization, the synthesized composite electrodes exhibited one and a half times higher NaCl removal capacity and three times higher adsorption rate as compared to that of a conventional CDI cell using commercial ion exchange membranes (IEMs) with almost the same energy consumption. The enhanced desalination performance was attributed to the optimized properties of the selected polymers and the improved adhesion of IEMs to the electrodes. This research paves the way for the application of new materials in MCDI processes for improved water desalination.     

Adsorption of perfluorinated compounds on thin‐film composite polyamide membranes

The adsorption behavior of perfluorinated compounds (PFCs), of various chain lengths and two different functional groups, on widely used thin‐film composite polyamide membranes has been investigated. Three commercially available polyamide membranes and two classes of PFCs were evaluated: Dow‐Filmtech BW30, NF90, and NF270 membranes; perfluorosulfonic and perfluoroalkanoic acid with 5, 7, 9, and 11 carbon atoms. The adsorption of PFCs on the membranes strongly depended on the active skin‐top layer material of the membranes, solution chemistry, and structure of PFCs. The piperazine based NF270 membrane showed higher adsorption of PFCs compared to BW30 and NF90 membranes (FT‐30 type membranes). The BW30 membrane, which has a coating layer of aliphatic carbons and hydroxyl groups on the surface of the polyamide substrate, had less interaction with PFCs than the NF90 polyamide membrane had. The adsorption of PFCs increased with increasing ionic strength, decreasing pH, and in divalent ion solutions. PFCs with longer chain lengths and more hydrophobic functional groups showed more attractive interactions with thin‐film composite membranes and, thus, greater adsorption on the membranes. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012     

纳米银在水处理膜中的应用进展

水处理膜是具有选择性分离功能的材料,利用水处理膜的选择性分离可实现污水的不同组分的分离、纯化、浓缩。基于纳米银的独特性质,将其引入水处理膜可使纳米银复合膜具有较强的抗生物污染能力。本文综述了近年来将纳米银引入应用较广泛的微滤膜、超滤膜和纳滤膜以及反渗透膜这些水处理膜中以改善膜的抗污染性能的研究进展。此外,指出了提高纳米银水处理复合膜抗菌的持续性以及开发环境友好型的复合膜是未来的发展方向。     

PVA@tea tree oil-emulsion core–shell microfiber membranes for extending the shelf life of fruits

In this work, a temperature-regulating and antimicrobial–antioxidant fiber membrane was fabricated using coaxial electrospinning technology. The tea tree oil (TTO) emulsion used as the core solution, polyvinyl alcohol (PVA) as the shell solution for coaxial electrospinning, has been demonstrated. Compared with the original PVA fibers, the average diameter of the fibers is increased after coaxial electrospinning, and the diameter increases with the core flow rate. The diameters corresponding to core flow rates of 0.05, 0.1, and 0.15 mL/h are 561, 597, and 849 nm, respectively. Via in vitro release experiment demonstrated that the quantity of TTO released from the composite membrane increased with the elevation of temperature. The inclusions TTO and polyethylene glycol give the film excellent antibacterial–antioxidant properties and a delayed thermal response. More intriguingly, core–shell fiber membranes containing TTO in the core part are effective in slowing down the formation of oxidation spots on the surface of banana compared with pure PVA membranes. Seven days after wrapping bananas with different membranes, the surface of banana in pure PVA film was full of oxidized spots, whereas banana in PVA core–shell fibers showed only one obvious spot.     

Preparation, morphology and performance evaluation of polyvinylalcohol (PVA)/polyethersulfone (PES) composite nanofiltration membranes for pulp and paper wastewater treatment

In this study, thin film composite PVA/PES nanofiltration membranes were fabricated for the treatment of pulp and paper industrial wastewater. Phase separation induced by immersion precipitation was used to prepare the PES support membrane. PVA/PES composite nanofiltration membranes were prepared by dipping the support PES membrane in the PVA and cross-linking solutions at different conditions. Maleic acid (MA) was used as cross-linking agent. PVA and MA have concentrations of 0.5?C2 and 0.05?C1 wt%, respectively. Morphological studies were carried out by means of scanning electron microscopy (SEM) as well as atomic force microscopy (AFM) techniques. In addition, the hydrophilicity of membranes was examined by contact angle measurements. Permeability and ability of PVA/PES composite nanofiltration membranes to reduce COD of the wastewater were evaluated by a cross flow filtration system. SEM images indicated that the PVA layer was uniformly formed on the PES support membrane. AFM images showed that the surface roughness, porosity and pore sizes of PES support membrane were reduced after formation of PVA layer on the support surface. Moreover, the hydrophilicity of the membranes was significantly increased. Experimental results demonstrated that the PVA/PES composite nanofiltration membranes were able to reduce the COD of wastewater. Optimum conditions for preparation of PVA/PES composite membrane are consisted of PVA concentration: 1 wt%, MA concentration: 0.5 wt%, cross-linking time: 3?min and curing time: 3?min.     

Spectroscopic and microscopic studies of vesicle rupture by AgNPs attack to screen the cytotoxicity of nanomaterials

It is well-known that silver nanoparticles (AgNPs) with 1–10 nm of diameter attached to the surface of cell membrane were able to penetrate into the cell followed by rupturing of the cell. However, most studies for cytotoxicity used living organisms and were time-consuming. Therefore, in this study, we proposed a model method for fingerprinting of nanotoxicity of AgNPs in mimetic cell membranes using phosphor lipid. The vesicle rupturing mechanism by AgNPs attack was investigated using surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM).