双水相萃取技术进展及其应用概况

双水相萃取技术是一种新型的生物分离技术,它与传统的萃取及其它分离技术相比具有操作条件温和、处理量大、易于连续操作等优点,从而使其能广泛应用于生物分离工程中。介绍了双水相的形成、双水相萃取技术的基本原理以及影响物质分配系数的因素。同时对双水相萃取技术的研究进展及其应用进行了综述,指出目前双水相萃取技术的研究进展集中表现在:廉价双水相体系的开发、新的双水相体系探索、双水相萃取技术同其它技术集成化、双水相萃取相关理论的进展等方面。双水相萃取的应用领域除了传统的生物分离领域外,还应用于金属离子的测定和分离等其它领域。     

双水相萃取技术研究进展及应用

双水相萃取技术作为一种新型的分离技术日益受到重视，它与传统的萃取方法相比有独特的优点。总结了双水相萃取形成的原理，萃取过程的基本理论、萃取体系的特点以及热力学模型的发展，综述了双水相萃取技术在生化工业、分析检测、稀有金属分离等方面的应用，介绍了该技术的最新进展，指出了该技术工业存在的问题并对今后的发展作了展望。     

双水相萃取技术研究进展

综述了双水相萃取技术的基本原理、特点、工艺流程、物质分配平衡的影响因素及其在生命科学,复杂中药体系的分离以及重金属回收等方面的应用.并对双水相萃取技术的发展前景作了展望.     

双水相萃取技术在生物技术上的应用

双水相萃取技术与传统的萃取及其他分离技术相比具有操作条件温和、处理量大、易于连续操作等优点,从而使其能广泛应用于生物技术领域。文章介绍了双水相萃取的作用原理,综述了近年来双水相萃取技术在生物技术中的应用,展望了双水相体系的应用前景。     

双水相萃取技术及其应用

双水相萃取技术作为一项新的分离技术日益受到重视,它与传统的萃取方法相比有独特的优点。本文综述了双水相萃取技术基本原理、特点、工艺流程及其若干应用,并对双水相萃取技术存在的问题和发展方向作了扼要论述。     

双水相萃取技术在分离、纯化中的应用

双水相技术是一种新型的液-液萃取技术,由于其条件温和、易操作等特点,目前已广泛应用于物质的分离、纯化。本文综述了双水相形成原理、工艺流程和特点、体系类别、影响双水相分配的因素及其在分离纯化中的应用,并针对其未来发展趋势进行了展望。     

双水相萃取技术研究现状

近年来,双水相萃取(ATPE)已经发展成由计算机控制、在常规萃取设备中、能连续操作的分离技术。介绍了传统双水相体系(ATPS)和新型ATPS的特征、分相机理、工业应用的优缺点,指出寻找低成本易回收可循环利用的ATPS组成物质和对预分离物质有更高选择性的ATPS组成物质是研发新型ATPS的方向。论述了ATPS液液平衡的热力学模型、分配系数的关联方法,指出提高选择性及分离效率是ATPE应用研究的方向,指出总结分配系数影响因素规律、修正液液平衡的热力学模型是ATPE理论研究的方向。     

双水相萃取技术研究进展

双水相萃取技术作为一种新型的分离技术日益受到重视,与传统的萃取及其他分离技术相比具有操作条件温和、处理量大等优点,从而使其能广泛应用于生物工程、药物分析等领域。本文主要介绍了双水相萃取的工艺流程,综述了近年来双水相萃取技术在生物技术、医药学、离子分离等方面的应用,展望了双水相体系的应用前景。     

双水相萃取技术研究进展

双水相萃取技术作为一项新的分离技术日益受到重视,它与传统的萃取方法相比有独特的优点。本文综述了双水相萃取技术基本原理、特点、应用及热力学模型,并对双水相萃取技术存在的问题和发展趋势作了论述。     

双水相萃取技术研究进展

双水相萃取技术作为一项新的分离技术日益受到重视,它与传统的萃取方法相比有独特的优点,作者综述了又水相萃取技术基本原理、特点、应用及热力学模型,并对双水相萃取技术存在的问题和发展趋势作了论述.     

双水相萃取技术及其在药物分离中的应用

双水相萃取技术作为一种新型的分离技术用于药物提取分离具有广阔前景。介绍了双水相体系的组成,双水相萃取技术原理、工艺过程、应用等。     

双水相体系萃取木瓜蛋白酶的研究

采用聚乙二醇(PEG)/(NH4)2SO4双水相体系对木瓜蛋白酶进行萃取分离,研究了PEG相对分子量、PEG质量分数、(NH4)2SO4质量分数和pH值对木瓜蛋白酶分配系数及酶活力回收率的影响.结果表明,最佳萃取条件为:PEG4000质量分数6%、(NH4)2SO4质量分数18%、pH值6.0,在此条件下,木瓜蛋白酶的...     

Extraction of Puerarin using Ionic Liquid Based Aqueous Two-Phase Systems

Various ionic liquid-based aqueous two-phase systems (IL-ATPS) were evaluated to extract puerarin. The results indicated that the nature of ILs, the salting-out ability of salt, and the acidity and basicity of the salt-rich solution had important influence on the extraction efficiency. Various factors were optimized systematically, that is, the amount of IL, salt, puerarin, and short-chain alcohol. Under the optimal experimental conditions (the amount of K₂HPO₄ 0.30–0.42 g/mL, [Bmim]Br 0.30–0.36 g/mL and 1.0 mL puerarin stock solution), the extraction efficiency of puerarin was over 99% by a single-step extraction, which indicated that the evaluated IL-ATPS was a prospective extraction medium. Finally, the IL-ATPS was successfully used to extract puerarin from Radix Puerariae Lobatae extracts.     

Application of Water-Miscible Alcohol-Based Aqueous Two-Phase Systems for Extraction of Dyes

The water-miscible alcohols (ethanol, 1-propanpol, 2-propanol) were used to form aqueous two-phase systems with K₂HPO₄ and Na₂HPO₄. Meanwhile, the organic citrates were also investigated as the phase-separation salts. The effects of the types and concentrations of phase-forming substances as well as temperature on the formation of aqueous two-phase systems were discussed. The alcohol (ethanol, 1-propanpol, 2-propanol)–salt (K₂HPO₄, K₃C₆H₅O₇, (NH₄)₃C₆H₅O₇) aqueous two-phase systems were used to extract Rhodamine B and methyl orange in aqueous solution. It proved that the water-miscible alcohol–salt (inorganic salt, organic salt) aqueous two-phase systems can be both used to extract hydrophilic targets directly without adding any reaction reagents. The aqueous two-phase extraction process was also discussed.     

双水相萃取法应用于从白萝卜中快速提取过氧化物酶

基于双水相萃取法在化工分离上的优点,研究了其应用于从白萝卜中快速提取过氧化物酶的可行性。结果表明,相对于传统盐析法,应用聚乙二醇/硫酸铵双水相萃取法从白萝卜中提取过氧化物酶具有快速、操作简单、提取率高、成本低及可以使生产规模化等优点。本研究对于过氧化物酶制剂的工业化生产或开发利用白萝卜等具有重要意义。     

Extraction and Recovery of Rutin from Acerola Waste using Alcohol-Salt-Based Aqueous Two-Phase Systems

Extraction of rutin from acerola waste was investigated using alcohol-salt-based aqueous two-phase systems (ATPS). Initially, the partitioning was studied using model systems with pure and commercial rutin. The impact of the ATPS constituents and composition, initial amount of rutin, temperature and addition of electrolytes was evaluated. Rutin can be recovered either in the alcohol-or-salt-rich phase depending on the salt used. To validate the optimization process, rutin extraction from acerola waste was carried out further. The results obtained with the real samples are in close agreement with the model systems and validate the optimization tests and support their applicability in bioresource-related processes.     

Aqueous Two-Phase Extraction of Lactic Acid: Optimization by Response Surface Methodology

In this study a suitable alcohol/salt aqueous two-phase (ATP) system was selected for the recovery of lactic acid from an aqueous solution. From the different ATP systems studied, the ethanol/dipotassium hydrogen phosphate ATP system appeared to be favorable. To examine the potential of this ATP system, the extraction yield of lactic acid in aqueous solutions was optimized with the response surface methodology. The parameters studied were concentrations of ethanol (22.00–38.80%, w/w), dipotassium hydrogen phosphate (15.00–31.80%, w/w) and lactic acid (26.36–93.64 g/L). The optimum conditions were found to be 30.23% w/w ethanol, 18.40% w/w dipotassium hydrogen phosphate, and 80 g/L lactic acid. Under these conditions, a favorable extraction yield of lactic acid was obtained. The maximum partition coefficient of lactic acid and extraction yield was determined as 2.26 and 87%, respectively. The optimum extraction conditions were then used to guide the recovery of lactic acid from a real fermentation broth. As a result, the partition coefficient and extraction yield of lactic acid reached 2.06–80%, respectively.     

两种双水相体系提取枇杷叶中黄酮的工艺研究

以枇杷叶为原料,利用双水相体系提取黄酮。考察了聚乙二醇-硫酸铵体系和乙醇-硫酸铵体系中各物质用量、料液比(g∶mL,下同)、pH值、提取温度、提取时间等条件对提取效率的影响,并对两种体系的提取效率进行了比较。结果表明:聚乙二醇-硫酸铵体系中,在聚乙二醇用量为2.0g、硫酸铵用量为1.5g、料液比为0.3∶10、pH值为9、提取温度为40℃、提取时间为1.5h的条件下,提取效率最高,达5.80%;乙醇-硫酸铵体系中,在无水乙醇用量为4.0mL、硫酸铵用量为1.8g、料液比为0.2∶10、提取温度为40℃、提取时间为1.0h的条件下,提取效率最高,达7.49%;可见乙醇-硫酸铵体系的黄酮提取效率高于聚乙二醇-硫酸铵体系。