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研究了高效液相色谱法(HPLC)测定生物柴油生产中副产物甘油的方法.生物柴油废弃物(主要含甘油)经稀释、调节pH至5.5,离心,所得下层液经微孔滤膜过滤,HPLC检测其甘油含量.最佳色谱条件色谱柱ZORBAX NH2(250 mm×4.6 mm,5 μm);检测器示差检测器;柱温30℃;流动相70%乙腈;流速1 mL/min.甘油最低检测限为2 mg/L,回收率为94.34%~99.43%,相对标准偏差为1.03%.与传统化学方法相比,高效液相色谱法快速简便,结果准确,重现性好. 相似文献
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结合国内外研究发展现状,归纳了影响酶法合成1,3-二油酸-2-棕榈酸甘油三酯的因素,包括脂肪酶、反应底物、反应器及反应工艺。指出了当前工艺存在的问题并提出了建议。 相似文献
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Marta Semkiv Iwona Kata Orysya Ternavska Wladimir Sibirny Kostyantyn Dmytruk Andriy Sibirny 《Yeast (Chichester, England)》2019,36(5):329-339
Production of fuel ethanol is one of the possible ways to utilize crude glycerol, substantial amounts of which are produced by biodiesel industry. Earlier, we have described construction of the recombinant strains of methylotrophic thermotolerant yeast Ogataea polymorpha with simultaneous overexpression of the genes PDC1 and ADH1, which produced increased amounts of ethanol from glycerol. In this work, we have further improved these strains by overexpression of genes involved either in oxidative (through dihydroxyacetone) or phosphorylative (through glycerol-3-phosphate) pathway of glycerol catabolism, as well as heterologous gene coding for glycerol transporter FPS1 from Komagataella phaffii (formerly, Pichia pastoris). Obtained recombinant strains produced up to 10.7 g/L of ethanol (with ethanol productivity 30 mg/g of biomass/hr and yield 132 mg/g of consumed glycerol) from pure glycerol and up to 3.55 g/L of ethanol (with ethanol productivity 11.6 mg/g of biomass/hr and yield 72.3 mg/g of consumed glycerol) from crude glycerol as a carbon source, which is approximately 15 times more relative to that of the O. polymorpha wild-type strain and 2.2 more relative to the earlier constructed strain. 相似文献
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Non-enzymic browning (NEB) reactions between glycerol and some of the amino acids present in meat have been observed at 38 and 65°C; l-lysine is the most reactive of the amino acids studied and l-cysteine and l-arginine the least. These NEB reactions also occur between glycerol and proteins (gelatin and casein). The reactions are not unique to glycerol as other polyhydric alcohols also form brown pigments with lysine. On prolonged air storage at 65°C glycerol itself undergoes mild oxidation, ultimately yielding a brown solution. 相似文献