Effects of Seed Color and Growing Locations on Fatty Acid Content and Composition of Two Chia (Salvia hispanica L.) Genotypes

The objective of this study was to investigate the effect of chia (Salvia hispanica L.) seed coat color on oil content and fatty acid composition, as well as the effect of different growing areas on chemical variation. This study was carried out using white and black-spotted chia seeds grown together at five locations of Ecuador. Oil content was not significantly (P < 0.05) different for any of the comparative analyses performed between white and black-spotted seeds at all, although significant differences in oil content among locations were detected. The seeds from the San Pablo location showed the highest oil concentration (34.5%). No significant differences among fatty acids at any of the location were detected between white and black-spotted seeds; however, significant differences in fatty acids composition between sites were found. Overall, significant (P < 0.05) differences in palmitic, oleic, linoleic, and α-linolenic fatty acid compositions among oils from seeds grown in different locations were detected. In conclusion, this paper shows that the larger differences found in oil content and fatty acid composition are due to location (because of the environmental differences) rather than chia seed coat color.     

<Emphasis Type="Italic">cis</Emphasis>-, <Emphasis Type="Italic">trans</Emphasis>- and Saturated Fatty Acids in Selected Hydrogenated and Refined Vegetable Oils in the Indian Market

The fatty acid composition of 27 samples of commercial hydrogenated vegetable oils and 23 samples of refined oils such as sunflower oil, rice bran oil, soybean oil and RBD palmolein marketed in India were analyzed. Total cis, trans unsaturated fatty acids (TFA) and saturated fatty acids (SFA) were determined. Out of the 27 hydrogenated fats, 11 % had TFA about 1 % where as 11 % had more than 5 % TFA with an average value of about 13.1 %. The 18:1 trans isomers, elaidic acid was the major trans contributor found to have an average value of about 10.8 % among the fats. The unsaturated fatty acids like cis-oleic acid, linoleic acid and α-linolenic acid were in the range of 21.8–40.2, 1.9–12.2, 0.0–0.7 % respectively. Out of the samples, eight fats had fatty acid profiles of low TFA (less than 10 %) and high polyunsaturated fatty acids (PUFA) such as linoleic and α-linolenic acid. They had a maximum TFA content of 7.3 % and PUFA of 11.7 %. Among the samples of refined oils, rice bran oil (5.8 %) and sunflower oil (4.4 %) had the maximum TFA content. RBD palmolein and rice bran oils had maximum saturated fatty acids content of 45.1 and 24.4 % respectively. RBD palmolein had a high monounsaturated fatty acids (MUFA) content of about 43.4 %, sunflower oil had a high linoleic acid content of about 56.1 % and soybean oil had a high α-linolenic acid content of about 5.3 %.     

Characterization of sacha inchi (<Emphasis Type="Italic">Plukenetia volubilis</Emphasis> L.) oil by FTIR spectroscopy and <Superscript>1</Superscript>H NMR. Comparison with linseed oil

Three oil samples obtained from sacha inchi (Plukenetia volubilis L.) seeds were studied by means of FTIR and ¹H NMR. Frequency data of the most significant bands of the IR spectrum of this oil are given. These data show that sacha inchi oil has a high degree of unsaturation. The same fact is deduced from the ratio between the absorbance of the bands due to the stretching vibrations of the cis olefinic CH double bonds at 3010.5 cm⁻¹ and to the methylene symmetrical stretching vibrations at 2855.1 cm⁻¹. The proportions of monounsaturated, polyunsaturated, and saturated acyl groups were predicted from the frequency of some IR bands, and these were in satisfactory agreement with the values obtained through FAME generation and their quantification by GC. Likewise, simple observation of the ¹H NMR spectra provided a great deal of information about sacha inchi oil, with regard not only to the relative proportions of the different acyl groups but also to their nature. Thus, the presence of γ-linolenic acyl groups was discounted. Furthermore, the area of some ¹H NMR signals was used to determine the proportion of saturated and mono-, di-, and triunsaturated acyl groups, which also were in satisfactory agreement with the values obtained by classical methods. IR and ¹H NMR determinations take very little time in comparison with classical methods and do not require chemical manipulation or transformation of the sample. A comparison was also made between the compositions of sacha inchi and linseed oil. Both oils are important sources of the healthful n−3 linolenic acyl groups, and sacha inchi also contains high proportions of the n−6 linoleic acyl groups.     

不同植物油脂对冷制皂入皂特性的影响

选用椰子油、棕榈油、橄榄油、山茶油、乳木果油、甜杏仁油、美藤果油和霍霍巴油8种植物油脂为原料,采用冷制工艺制作成3组不同含油量的植物油脂手工皂,研究了不同植物油脂对冷制皂入皂特性的影响,并对植物油脂复配制作的冷制皂进行肤感感官评价。结果表明,植物油脂的种类和含量对冷制皂的p H值影响不大,对其他入皂特性均有影响。椰子油冷制皂硬度高、起泡性强且泡沫丰富、Trace time短,与不饱和植物油脂复配入皂有助于缩短制作冷制皂的Trace time,提高冷制皂的硬度和起泡能力;山茶油、甜杏仁油和橄榄油入皂有助于起泡;橄榄油入皂有助于提高冷制皂的泡沫稳定性;乳木果油和霍霍巴油入皂起泡能力较差;美藤果油入皂滋润度高、泡沫细腻、洗感舒适、肤感评价最佳。     

Untersuchungen über Anbaueignung und Qualitätseigenschaften von Oenothera biennis L.

Investigations in Cultivation Abilities and Seed Quality of Oenothera biennis L. Due to relatively high concentration of γ-linolenic acid seed oil of evening primrose (Oenothera biennis L.) is requested for cosmetian and clinical applications. Variation of oil content and concentration of γ-linolenic acid in the seeds of 50 single wild plants and their selected progenies from different locations were determined. In a phytotron experiment the influence of temperature and nitrogen fertilization on oil content and fatty acid composition were examined. Contrary to other oil plants in evening primrose no influence of different temperature on desaturation degree of the fatty acids – especially on γ-linolenic acid concentration was detectable.     

Characteristics and composition of melon and grape seed oils and cakes

Watermelon (Citrullus vulgaris) and grape (Vitis vinifera) seeds were investigated for their nutritional quality and oil characteristics. The yields of seeds on an as is basis (edible portion) were 1.6 and 1.8% for grape and melon, respectively. The melonseed on a dry weight basis consisted of 53.6% testa and 46.4% kernel. The crude protein, fat and fiber content were 16.4, 23.1 and 47.7% for melon and 8.2, 14.0 and 38.6% for grape (dry weight basis). Both seeds were found to contain significant levels of Ca, Mg, P and K. The fatty acid profiles showed an unsaturated fatty acid content of 76.1% for melonseed oil and 88.6% for grapeseed oil. The predominant fatty acid in both seeds was linoleic acid. The iodine value, saponification number and acid value were 116, 248 and 0.97 for melonseed oil and 132, 194 and 1.59 for grapeseed oil. The amino acid profiles of both seed cake proteins were determined and compared with hen’s egg protein.     

Genomic Regions Governing the Biosynthesis of Unsaturated Fatty Acids in Recombinant Inbred Lines of Soybean Raised across Multiple Growing Years

The ratio of oleic acid to the combined value of linoleic and α-linolenic acids determines the oxidative stability, and the ratio of linoleic acid to α-linolenic acid is the key to the nutritional value of soybean oil. The present study was conducted to identify genomic regions associated with oleic, linoleic, and α-linolenic acids in recombinant inbred lines (RIL), developed from LSb1 × NRC7, across 5 cropping years. These RIL were genotyped using 105 polymorphic SSR markers across soybean genome and analyzed for fatty acid composition. SSR markers, namely, Satt245 (LGp M), Satt556 (LGpB2), Sat_042 (LGp C1), Staga002 (LGp D1b), Satt684 (LGp A1), and AI856415 (LGpD1b) showed significant (P < 0.05) association with oleic acid for all the 5 years, though this association was weak in the years when the growing temperature during active seed formation stage was high. Quantitative trait loci (QTL) linked to Satt684 (LGp A1), Satt556 (LGp B2), Sat_042 (LGp C1), and AI856415 (LGp D1b) showed pleiotropic influence on the levels of unsaturated fatty acids. Complementation of favorable QTL from LSb1 and NRC7 generated 60% oleic acid and less than 4% α-linolenic acid RIL, stable across 5 cropping years. New SSR markers, namely, Satt245, AI856415, and Staga002 identified to be associated with different unsaturated fatty acids may be useful in improving the efficiency of marker-assisted breeding for enhancing the monounsaturated to polyunsaturated fatty acids ratio of soybean oil.     

Identification and Functional Characterization of Acyl-ACP Thioesterases B (GhFatBs) Responsible for Palmitic Acid Accumulation in Cotton Seeds

In spite of increasing use in the food industry, high relative levels of palmitic acid (C16:0) in cottonseed oil imposes harmful effects on human health when overconsumed in the diet. The limited understanding of the mechanism in controlling fatty acid composition has become a significant obstacle for breeding novel cotton varieties with high-quality oil. Fatty acyl–acyl carrier protein (ACP) thioesterase B (FatBs) are a group of enzymes which prefer to hydrolyze the thioester bond from saturated acyl-ACPs, thus playing key roles in controlling the accumulation of saturated fatty acids. However, FatB members and their roles in cotton are largely unknown. In this study, a genome-wide characterization of FatB members was performed in allotetraploid upland cotton, aiming to explore the GhFatBs responsible for high accumulations of C16:0 in cotton seeds. A total of 14 GhFatB genes with uneven distribution on chromosomes were identified from an upland cotton genome and grouped into seven subfamilies through phylogenetic analysis. The six key amino acid residues (Ala, Trys, Ile, Met, Arg and Try) responsible for substrate preference were identified in the N-terminal acyl binding pocket of GhFatBs. RNA-seq and qRT-PCR analysis revealed that the expression profiles of GhFatB genes varied in multiple cotton tissues, with eight GhFatBs (GhA/D-FatB3, GhA/D-FatB4, GhA/D-FatB5, and GhA/D-FatB7) having high expression levels in developing seeds. In particular, expression patterns of GhA-FatB3 and GhD-FatB4 were positively correlated with the dynamic accumulation of C16:0 during cotton seed development. Furthermore, heterologous overexpression assay of either GhA-FatB3 or GhD-FatB4 demonstrated that these two GhFatBs had a high substrate preference to 16:0-ACP, thus contributing greatly to the enrichment of palmitic acid in the tested tissues. Taken together, these findings increase our understanding on fatty acid accumulation and regulation mechanisms in plant seeds. GhFatBs, especially GhA-FatB3 and GhD-FatB4, could be molecular targets for genetic modification to reduce palmitic acid content or to optimize fatty acid profiles in cotton and other oil crops required for the sustainable production of healthy edible oil.     

Comparative Analysis of Essential Bioactive Components of Oils Originating from Three Chinese Loess Plateau Wild Crops

The ability of soil and water conservation crops to resist stress is closely related to their abundance of lipid-soluble chemical components. This study systematically evaluated the composition and content of fatty acids, sterols, squalene, and tocopherol in oils extracted from three varieties of crops growing on the Chinese Loess Plateau with extreme environments. The dominant fatty acids in the wild seabuckthorn pulp oil were oleic acid (29.73%), palmitic acid (26.83%), and palmitoleic acid (25.71%), and those in wild seabuckthorn seed oil were linoleic acid (42.29%), α-linolenic acid (20.65%), and oleic acid (18.94%). The most abundant fatty acids in wild elaeagnusmollis seed oil were oleic acid (43.29%), linoleic acid (35.93%), and α-linolenic acid (7.00%). Wild yellowhorn seed oil was rich in linoleic acid (34.14%), oleic acid (25.99%), and erucic acid (8.76%). Seabuckthorn seed oil had the highest levels of total sterols (619.33 mg/100 g), followed by seabuckthorn pulp oil (606.10 mg/100 g), yellowhorn seed oil (249.46 mg/100 g), and elaeagnusmollis seed oil (224.01 mg/100 g). However, the squalene content was highest in elaeagnusmollis seed oil (68.06 mg/100 g) and similarly low in yellowhorn seed oil (9.81 mg/100 g), seabuckthorn pulp oil (4.62 mg/100 g) and seabuckthorn seed oil (4.71 mg/100 g). In addition, seabuckthorn pulp oil had the highest tocopherol content (179.92 mg/100 g), followed by seabuckthorn seed oil (130.57 mg/100 g), elaeagnusmollis seed oil (85.87 mg/100 g), and yellowhorn seed oil (45.44 mg/100 g). This study provides favorable data supporting biomass resource utilization and organic synthesis of bioactive raw chemical composition.     

Effect of 1-naphthaleneacetic acid on the seed,protein, oil,and fatty acids of egyptian cotton

The effects of 1-naphthaleneacetic acid (NAA) applied at various levels and times on yield, seed index, protein and oil content and fatty acid compositions of cotton plants seeds were studied. NAA increased the seed yield/plant and the seed, protein, and oil yields/ha compared to the control. A level of 20 ppm proved best for yield. Most NAA treatments significantly increased the seed index, but only slight increases in seed protein content were recorded. Increases in the amount of seed oil was moderate, with fluctuation that was due to treatment. NAA generally caused decreases in saturated fatty acids that were associated with increases in unsaturated fatty acids. One treatment proved to be more effective than multiple treatments. Palmitic acid and linoleic acid were the most abundant saturated and unsaturated fatty acids, respectively.     

Oil and Conjugated Linolenic Acid Contents of Seeds from Important Pomegranate Cultivars (<Emphasis Type="Italic">Punica granatum</Emphasis> L.) Grown in Turkey

In the present study, seed oil content and fatty acid composition of 15 commercially important pomegranate cultivars were determined. The oil content of pomegranate seeds ranged between 13.95 and 24.13% (d.b). Palmitic, stearic, arachidic, and behenic acid contents of the oils ranged between 2.10–2.77, 1.35–2.01, 0.33–0.48, and 0.16–0.22%, respectively. The predominant unsaturated fatty acid was punicic acid (70.42–76.17%) and a minor unsaturated fatty acid was gadoleic acid (0.42–0.75%). The analysis on unsaturated fatty acids particularly showed significant amounts of punicic acid, which is considered to enhance the oil quality and is of importance to health.     

Lipid of sunflower seeds produced in japan

The fatty acid composition and tocopherol content of sunflower seeds from experimental plantings in Japan were determined. Lipid content of sunflower seed was almost the same irrespective of the variety and the average lipid content was 38.8%. The saturated fatty acids were low and the combined percentage of linoleic acid and oleic acid was ca. 90%. The ratio of oleic acid to linoleic acid largely varied with the planting location and date. There was a strong correlation between the percentage of linoleic acid or oleic acid and the temperature during maturation of seed. The sunflower seed contained predominantly α-tocopherol and small amounts ofβ-,γ- and δ-tocopherol. There was no correlation between a-tocopherol content and the percentage of linoleic acid.     

α-Linolenic Acid Transfer into Milk Fat and its Elongation by Cows

It was the purpose of this study ot explore to what extent α-linolenic acids transferred into bovine milk fat if either fed as intact linseed or if infused as linseed oil (α-linolenic acid content ≈? 55 wt%) into the abomasum. Two cows consumed 2000 g/d linseed for 9wk. Then two doses of linseed oil (500 or 250 g/d) were infused for 14 or 4 wk into the abomasum of the cows, respectively. There was no effect of linseed oil on milk production, milk fat and protein content, whereas linseed caused a slight decline of milk protein content simultaneously with a pronounced rise of trans fatty acids in milk fat. While during the linseed period only 1% of consumed α-linolenic acid (C18:3n-3) appeared in milk fat, on average 49 or 56% of infused C15:3 was transferred into milk fat depending on the infusion rate of linseed oil. There was only modest rise of about 0.25 wt% of desaturation/elongation metabolites (C20:5 plus C22:5) of α-linolenic acid in milk fat indicating a low δ⁶-desaturase activity of the cow. Docosahexaenoic acid (C22:6) was not found in milk fat. It is concluded that bovine lipid metabolism allows surprisingly high transfer rates of n-3 fatty acids of linseed oil into milk fat and that there is the possibility to enrich milk fat with n-3 fatty acids without affecting milk yield provided ruminal biohydrogenation is prevented.     

Determination of Oil Content and Fatty Acid Composition of Sesame Mutants Suited for Intensive Management Conditions

Sesame mutants with closed capsules, determinate growth habit and wilt resistance, have been proposed to be suitable for intensive management conditions facilitating mechanized harvesting. The objective of our experiment was to determine the oil content and fatty acid composition of these mutants before they are placed on the market. Oil content and fatty acids were studied in 19 mutants, 6 breeding lines and 4 control source genotypes. The oil contents of the seeds ranged from 46.4 to 62.7%. The mutants had generally a lower oil content than the control genotypes except the wilting tolerant group. For unsaturated fatty acids, oleic acid was higher in the mutants and breeding lines while linoleic acid was lower in the seed oil. However, no mutants or breeding lines were found with radically different composition or with any undesirable lipid component. The closed capsule and determinate growth habit mutants need to be improved for oil content while their fatty acid composition is fine.