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1.
Setyaningrum Rahmawaty Karen Charlton Philippa Lyons-Wall Barbara J. Meyer 《Lipids》2013,48(9):869-877
Secondary analysis of the 2007 Australian National Children’s Nutrition and Physical Activity survey was undertaken to assess the intake and food sources of EPA, DPA and DHA (excluding supplements) in 4,487 children aged 2–16 years. An average of two 24-h dietary recalls was analysed for each child and food sources of EPA, DPA and DHA were assessed using the Australian nutrient composition database called AUSNUT 2007. Median (inter quartile range, IQR) for EPA, DPA and DHA intakes (mg/day) for 2–3, 4–8, 9–13, 14–16 year were: EPA 5.3 (1.5–14), 6.7 (1.8–18), 8.7 (2.6–23), 9.8 (2.7–28) respectively; DPA 6.2 (2.2–14), 8.2 (3.3–18), 10.8 (4.3–24), 12.2 (5–29) respectively; and DHA 3.9 (0.6–24), 5.1 (0.9–26), 6.8 (1.1–27), 7.8 (1.5–33) respectively. Energy-adjusted intakes of EPA, DPA and DHA in children who ate fish were 7.5, 2 and 16-fold higher, respectively (P < 0.001) compared to those who did not eat fish during the 2 days of the survey. Intake of total long chain n-3 PUFA was compared to the energy adjusted suggested dietary target (SDT) for Australian children and 20 % of children who ate fish during the 2 days of the survey met the SDT. Fish and seafood products were the largest contributors to DHA (76 %) and EPA (59 %) intake, while meat, poultry and game contributed to 56 % DPA. Meat consumption was 8.5 times greater than that for fish/seafood. Australian children do not consume the recommended amounts of long chain omega-3 fatty acids, especially DHA, which could be explained by low fish consumption. 相似文献
2.
本文评述了脂肪酶浓缩鱼油中EPA、DHA的几种方法,并介绍了DPA,DHA的分离提纯手段。 相似文献
3.
Inclusion of flaxseed in turkey diets decreases the n‐6/n‐3 PUFA ratio and increases the proportion of biologically active EPA and DHA without affecting meat quality 下载免费PDF全文
Jan Jankowski Zenon Zdunczyk Dariusz Mikulski Jakub Naczmanski Jerzy Juskiewicz Agnieszka Troszynska Bogdan A. Slominski 《European Journal of Lipid Science and Technology》2015,117(6):797-809
4.
Sylviane Tardivel Aurélie Gousset-Dupont Véronique Robert Marie-Luce Pourci Alain Grynberg Bernard Lacour 《Lipids》2009,44(3):225-235
Oxidized low density lipoprotein (Ox-LDL) is a well-established risk factor in atherosclerosis and lysophosphatidylcholine
(LysoPtdCho) is considered to be one of the major atherogenic component of Ox-LDL. The purpose of this work was to investigate
the effects of two membrane n-3 long chain polyunsaturated fatty acids (n-3 PUFAs), EPA (eicosapentaenoic acid) and DHA (docosahexaenoic
acid) compared to n-6 PUFA, ARA (arachidonic acid), on the activation of endothelial NO synthase (eNOS) by histamine in Ea
hy 926 endothelial cells incubated during 24 h in the presence or the absence of LysoPtdCho. DHA (50 μM) produced a ROS induction
in cells and aggravated the LysoPtdCho-induced oxidative stress. It did not modify the basal eNOS activity but impaired the
stimulation of eNOS induced by histamine and was unable to correct the deleterious effect of LysoPtdCho on histamine-stimulated
eNOS activity or phosphorylation of Ser 1177. In contrast, EPA (90 μM) did not modify the ROS level produced in the presence
or absence of LysoPtdCho or basal eNOS activity and the stimulating effect of histamine on eNOS. However, it diminished the
deleterious effect of LysoPtdCho as well as on the histamine-stimulated eNOS activity on the phosphorylation on Ser 1177 of
eNOS. The beneficial effect of EPA but not DHA on endothelial eNOS activity in Ea hy 926 could be also partially due to a
slight decrease in membrane DHA content in EPA-treated cells. Consequently, the equilibrium between NO generated by eNOS and
ROS due to oxidative stress could explain, in part, the beneficial effect of EPA on the development of cardiovascular diseases.
By contrast ARA an n-6 PUFA was devoid of any effect on ROS generation or eNOS activity in the basal state or after histamine-induced
stimulation. In vivo experiments should be undertaken to confirm these results. 相似文献
5.
Separation of EPA and DHA in fish oil by lipase-catalyzed esterification with glycerol 总被引:4,自引:0,他引:4
The objective of this study was to investigate the use of lipases as catalysts for separating EPA and DHA in fish oil by kinetic
resolution based on their FA selectivity. Esterification of FFA from various types of fish oils with glycerol by immobilized
Rhizomucor miehei lipase under water-deficient, solvent-free conditions resulted in a highly efficient separation of EPA and DHA. Reactions
were conducted at 40°C with a 10% dosage of the lipase preparation under vacuum to remove the coproduced water, thus rapidly
shifting the reaction toward the products. The bulk of the FA, together with EPA, were converted into acylglycerols, whereas
DHA remained in the residual FFA. As an example, when FFA from tuna oil comprising 5% EPA and 25% DHA were esterified with
glycerol, 90% conversion into acylglycerols was obtained after 48 h. The residual FFA contained 78% DHA and only 3% EPA, in
79% DHA recovery. EPA recovery in the acylglycerol fraction was 91%. The type of fish oil and extent of conversion were highly
important parameters in controlling the degree of concentration. 相似文献
6.
EPA,DHA, and Lipoic Acid Differentially Modulate the n-3 Fatty Acid Biosynthetic Pathway in Atlantic Salmon Hepatocytes 下载免费PDF全文
The aim of the present study was to investigate how EPA, DHA, and lipoic acid (LA) influence the different metabolic steps in the n‐3 fatty acid (FA) biosynthetic pathway in hepatocytes from Atlantic salmon fed four dietary levels (0, 0.5, 1.0 and 2.0%) of EPA, DHA or a 1:1 mixture of these FA. The hepatocytes were incubated with [1‐14C] 18:3n‐3 in the presence or absence of LA (0.2 mM). Increased endogenous levels of EPA and/or DHA and LA exposure both led to similar responses in cells with reduced desaturation and elongation of [1‐14C] 18:3n‐3 to 18:4n‐3, 20:4n‐3, and EPA, in agreement with reduced expression of the Δ6 desaturase gene involved in the first step of conversion. DHA production, on the other hand, was maintained even in groups with high endogenous levels of DHA, possibly due to a more complex regulation of this last step in the n‐3 metabolic pathway. Inhibition of the Δ6 desaturase pathway led to increased direct elongation to 20:3n‐3 by both DHA and LA. Possibly the route by 20:3n‐3 and then Δ8 desaturation to 20:4n‐3, bypassing the first Δ6 desaturase step, can partly explain the maintained or even increased levels of DHA production. LA increased DHA production in the phospholipid fraction of hepatocytes isolated from fish fed 0 and 0.5% EPA and/or DHA, indicating that LA has the potential to further increase the production of this health‐beneficial FA in fish fed diets with low levels of EPA and/or DHA. 相似文献
7.
Shu Yang Sun Yan Xu Dong Wang 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2009,84(3):435-441
BACKGROUND: Purification and characterization of an intracellular lipase produced by Rhizopus chinenesis cultured in solid‐state fermentation was investigated. The potential application in concentrating eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from fish oil by the pure enzyme was also studied. RESULTS: Through four successive purification steps, the enzyme was purified to homogeneity with an apparent molecular mass of 36 kDa. The lipase was active for pH between 7.0 and 9.0 and temperatures 20–45 °C. Lipase activity was slightly increased in the presence of Ca2+ and Mg2+, but strongly inhibited by Hg2+ and SDS. The pure enzyme was most active on medium chain p‐nitrophenol esters, with the highest activity towards pNP‐caprylate (C8). The enzyme is a non‐specific lipase, because it cleaved not only the 1,3‐positioned ester bonds but also the 2‐positioned bond in triolein. High EPA (17.6%) and DHA (32.9%) contents were achieved using the pure lipase (100 U) within 10 h. CONCLUSION: The enzymatic activity of the lipase on a wide variety of substrates and its stability in the presence of some organic solvents suggest that the lipase should be investigated for a range of commercial applications. The pure lipase was proved to possess potential ability for the production and concentration of EPA and DHA from fish oil. Copyright © 2008 Society of Chemical Industry 相似文献
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9.
The biosynthesis of DHA is increased in the liver of diabetic rats induced by high‐fat diets and STZ,in correlation with increased activity of peroxisomal β‐oxidation 下载免费PDF全文
Min Yao Lianguo Hou Tian Xie Yang Liu Dongxue Dai Yun Shi Kaoqi Lian Lingling Jiang 《European Journal of Lipid Science and Technology》2016,118(2):137-146