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1.
The patent-pending integrated waste-to-energy system comprises both a novel biohydrogen reactor with a gravity settler (Biohydrogenator), followed by a second stage conventional anaerobic digester for the production of methane gas. This chemical-free process has been tested with a synthetic wastewater/leachate solution, and was operated at 37 °C for 45 d. The biohydrogenator (system (A), stage 1) steadily produced hydrogen with no methane during the experimental period. The maximum hydrogen yield was 400 mL H2/g glucose with an average of 345 mL H2/g glucose, as compared to 141 and 118 mL H2/g glucose for two consecutive runs done in parallel using a conventional continuously stirred tank reactor (CSTR, System (B)). Decoupling of the solids retention time (SRT) from the hydraulic retention time (HRT) using the gravity settler showed a marked improvement in performance, with the maximum and average hydrogen production rates in system (A) of 22 and 19 L H2/d, as compared with 2–7 L H2/d in the CSTR resulting in a maximum yield of 2.8 mol H2/mol glucose much higher than the 1.1–1.3 mol H2/mol glucose observed in the CSTR. Furthermore, while the CSTR collapsed in 10–15 d due to biomass washout, the biohydrogenator continued stable operation for the 45 d reported here and beyond. The methane yield for the second stage in system (A) approached a maximum value of 426 mL CH4/gCOD removed, while an overall chemical oxygen demand (COD) removal efficiency of 94% was achieved in system (A). 相似文献
2.
Fermentative H2 production in continuous stirred tank reactor (CSTR) system with bacteria attached onto granular activated carbon (GAC) was designed to produce H2 continuously. The H2 production performances of CSTR with suspended and attached-sludge from molasses were examined and compared at various organic loading rates (8–40 g COD/L/d) at hydraulic retention time of 6 h under mesophilic conditions (35 °C). Both reactor systems achieved ethanol-type fermentation in the pH ranges 4.5–4.8 and 3.8–4.4, respectively, while ORP ranges from −450 to −470 mV and from −330 to −350 mV, respectively. The hydrogen production rate in the attached system was higher compared to that of the suspended system (9.72 and 6.65 L/d/L, respectively) while specific hydrogen production rate of 5.13 L/g VSS/d was higher in the suspended system. The attached-sludge CSTR is more stable than the suspended-sludge CSTR with regard to hydrogen production, pH, substrate utilization efficiency and metabolic products (e.g., volatile fatty acids and ethanol) during the whole test. 相似文献
3.
Eduardo Lucena Cavalcante de Amorim Aruana Rocha Barros Márcia Helena Rissato Zamariolli Damianovic Edson Luiz Silva 《International Journal of Hydrogen Energy》2009
This study evaluated hydrogen production in an anaerobic fluidized bed reactor (AFBR) fed with glucose-based synthetic wastewater. Particles of expanded clay (2.8–3.35 mm) were used as a support material for biomass immobilization. The reactor was operated with hydraulic retention times (HRT) ranging from 8 to 1 h. The hydrogen yield production increased from 1.41 to 2.49 mol H2 mol−1 glucose as HRT decreased from 8 to 2 h. However, when HRT was 1 h, there was a slight decrease to 2.41 mol H2 mol−1 glucose. The biogas produced was composed of H2 and CO2, and the H2 content increased from 8% to 35% as HRT decreased. The major soluble metabolites during H2 fermentation were acetic acid (HAc) and butyric acid (HBu), accounting for 36.1–53.3% and 37.7–44.9% of total soluble metabolites, respectively. Overall, the results demonstrate the potential of using expanded clay as support material for hydrogen production in AFBRs. 相似文献
4.
Mei-Ling Chong Raha Abdul Rahim Yoshihito Shirai Mohd Ali Hassan 《International Journal of Hydrogen Energy》2009
A hydrogen producer was successfully isolated from anaerobic digested palm oil mill effluent (POME) sludge. The strain, designated as Clostridium butyricum EB6, efficiently produced hydrogen concurrently with cell growth. A controlled study was done on a synthetic medium at an initial pH value of 6.0 with 10 g/L glucose with the maximum hydrogen production at 948 mL H2/L-medium and the volumetric hydrogen production rate at 172 mL H2/L-medium/h. The supplementation of yeast extract was shown to have a significant effect with a maximum hydrogen production of 992 mL H2/L-medium at 4 g/L of yeast extract added. The effect of pH on hydrogen production from POME was investigated. Experimental results showed that the optimum hydrogen production ability occurred at pH 5.5. The maximum hydrogen production and maximum volumetric hydrogen production rate were at 3195 mL H2/L-medium and 1034 mL H2/L-medium/h, respectively. The hydrogen content in the biogas produced was in the range of 60–70%. 相似文献
5.
Kyung-Won JungDong-Hoon Kim Hang-Sik Shin 《International Journal of Hydrogen Energy》2011,36(2):1466-1473
Introduction of an up-flow anaerobic sludge blanket (UASB) reactor apparatus to fermentative hydrogen production (FHP) enormously improves H2 production performance. However, the long start-up period required to form H2-producing granules (HPG) remains as a major obstacle. In the present work, a completely-stirred tank reactor (CSTR) was operated for 7 days, and the mixed liquor in the CSTR was transferred to a UASB reactor (UASBr (I)) as a seeding source. Coffee drink manufacturing wastewater (CDMW) was used as a feedstock, constituting the first attempt to form HPG from actual industrial wastewater. The strategy employed here was found to be more effective in developing HPG than directly starting from the UASB reactor (UASBr (II)), which is attributed to substantially higher active mass transfer in the CSTR. The average size of particles in the UASBr (II) blanket zone after 50 days of operation corresponded with that in the CSTR after only 7 days of operation. The drastic decrease of extracellular polymeric substance (EPS) protein concentration in the CSTR operation also indicates efficient removal of non-active biomass, the presence of which could adversely affect HPG formation. UASBr (I) showed a stable H2 yield and H2 production rate of 1.78 mol H2/mol hexoseadded and 2.76 L H2/L/h, respectively, and HPG with an average size of 1.9 mm were developed after 45 days. It appears that the abundant presence of divalent ions, especially calcium ions, contained in the CDMW facilitated HPG formation. 相似文献
6.
The phenomenon of bacterial wash-out frequently occurs in the traditional continuous stirred tank reactor (CSTR) systems at low hydraulic retention time (HRT). In this study, the effect of different aspect ratios, height (H) to diameter (D) of 1:1, 3:1 and 5:1, of a CSTR with immobilized anaerobic sludge on hydrogen (H2) production were investigated. The pH, volatile suspended solids (VSS) and total solids (TS) concentrations of the seed sludge were 6.8, 33.3 and 65.1 g/L, respectively. Thermally treated sludge was immobilized by silicone gel entrapment approach. The entrapped-sludge system operated stably at a low HRT without suffering from cell wash-out. Hence, the hydrogen production rate (HPR) was enhanced by increasing organic loading rates. The immobilized sludge CSTRs were operated at 40 °C with sucrose (10, 20, 30 and 40 g COD/L) and Endo nutrient medium at different HRTs (4, 2, 1 and 0.5 h). It was found that the granule formation enhanced HPR. The maximum HPR and the H2 yield were found to be 15.36H2 L/h/L and 3.16 mol H2/mol sucrose, respectively, with the H2 content in the biogas above 44% for all tests runs. 相似文献
7.
Continuous biohydrogen production with calcium supplementation at low hydraulic retention time (HRT) in a continuous stirred tank reactor (CSTR) was studied to maximize the hydrogen productivity of anaerobic mixed cultures. After stable operations at HRT of 8–4 h, the bioreactor became unstable when the HRT was lowered to 2 h. Supplementation of 100 mg/L calcium at HRT 2 h improved the operation stability through enhancement of cell retention with almost two-fold increase in cell density than that without calcium addition. Hydrogen production rate and hydrogen yield reached 24.5 L/d/L and 3.74 mol H2/mol sucrose, respectively, both of which were the highest values our group have ever achieved. The results showed that calcium supplementation can be an effective way to improve the performance of CSTR at low HRT. 相似文献
8.
S.Venkata Mohan G. MohanakrishnaS. Veer Raghavulu P.N. Sarma 《International Journal of Hydrogen Energy》2007
The basic aim of this study was to investigate the feasibility of bioaugmentation strategy in the process of enhancing biohydrogen (H2) production from chemical wastewater treatment (organic loading rate (OLR)—6.3 kg COD/m3-day) in anaerobic sequencing batch biofilm reactor (AnSBBR) operated at room temperature (28±2 °C) under acidophilic microenvironment (pH 6) with a total cycle period of 24 h. Parent augmented inoculum (kanamycin resistant) was acquired from an operating upflow anaerobic sludge blanket (UASB) reactor treating chemical wastewater and subjected to selective enrichment by applying repetitive/cyclic pre-treatment methods [altering between heat-shock treatment (100 °C; 2 h) and acid treatment (pH 3; 24 h)] to eliminate non-spore forming bacteria and to inhibit the growth of methanogenic bacteria (MB). Experimental data revealed the positive influence of bioaugmentation strategy on the overall H2 production. Specific H2 production almost doubled after augmentation from 0.297 to 0.483 mol H2/kg CODR-day. Chemical wastewater acted as primary carbon source in the metabolic reactions involving molecular H2 generation leading to substrate degradation. The augmented culture persisted in the system till the termination of the experiments. The survival and retention of the augmented inoculum and its positive effect on process enhancement may be attributed to the adopted reactor configuration and operating conditions. Scanning electron microscope (SEM) images documented the selective enrichment of morphologically similar group of bacteria capable of producing H2 under acidophilic conditions in anaerobic microenvironment. This depicted work corroborated successful application of bioaugmentation strategy to improve H2 production rate from anaerobic chemical wastewater treatment. 相似文献
9.
Bio-hydrogen production from thin stillage using conventional and acclimatized anaerobic digester sludge 总被引:1,自引:0,他引:1
Noha NasrElsayed Elbeshbishy Hisham Hafez George Nakhla M. Hesham El Naggar 《International Journal of Hydrogen Energy》2011,36(20):12761-12769
To assess the viability of biohydrogen production from thin stillage, a comparative evaluation of anaerobic digester sludge (ADS) and acclimatized anaerobic digester sludge (AADS) for biohydrogen production over a wide range of S0/X0 ratio (0.5-8 gCOD/gVSS) was performed. A maximum hydrogen yield of 19.5 L H2/L thin stillage was achieved for the AADS while tests with ADS achieved a maximum yield of only 7.5 L H2/L thin stillage. The optimum range of S0/X0 ratio for hydrogen production was found to be 1 to 2 gCOD/gVSS using conventional ADS and 3 to 6 gCOD/gVSS using AADS. The biomass specific hydrogen production rate for the AADS was 3.5 times higher than rate for the ADS throughout the range of S0/X0 ratio examined in this study. The DGGE profiles of the 16S rDNA gene fragments confirmed the superior performance of the AADS over the ADS, showing that the widely known hydrogen producers Clostridium acetobutyricum, Klebsiella pneumonia, Clostridium butyricum and Clostridium pasteurianum were the predominant species. 相似文献
10.
Hariklia N. Gavala Ioannis V. SkiadasBirgitte K. Ahring 《International Journal of Hydrogen Energy》2006
Biological production of hydrogen gas has received increasing interest from the international community during the last decade. Most studies on biological fermentative hydrogen production from carbohydrates using mixed cultures have been conducted in conventional continuous stirred tank reactors (CSTR) under mesophilic conditions. Investigations on hydrogen production in reactor systems with attached microbial growth have recently come up as well as investigations on hydrogen production in the thermophilic temperature range. The present study examines and compares the biological fermentative production of hydrogen from glucose in a continuous stirred tank type bioreactor (CSTR) and an upflow anaerobic sludge blanket bioreactor (UASB) at various hydraulic retention times (2–12 h HRT) under mesophilic conditions (35 °C). Also the biohydrogen production from glucose in the CSTR at mesophilic and thermophilic (55 °C) temperature range was studied and compared. From the CSTR experiments it was found that thermophilic conditions combine high hydrogen production rate with low production of microbial mass, thus giving a specific hydrogen production rate as high as 104 mmole H2/h/l/g VSS at 6 h retention time compared to a specific hydrogen production rate of 12 mmole H2/h/l/g VSS under mesophilic conditions. On the other hand, the UASB reactor configuration is more stable than the CSTR regarding hydrogen production, pH, glucose consumption and microbial by-products (e.g. volatile fatty acids, alcohols etc.) at the HRTs tested. Moreover, the hydrogen production rate in the UASB reactor was significantly higher compared to that of the CSTR at low retention times (19.05 and 8.42 mmole H2/h/l, respectively at 2 h HRT) while hydrogen yield (mmole H2/mmole glucose consumed) was higher in the CSTR reactor at all HRT tested. This implies that there is a trade-off between technical efficiency (based on hydrogen yield) and economic efficiency (based on hydrogen production rate) when the attached (UASB) and suspended (CSTR) growth configurations are compared. 相似文献
11.
A series of batch experiments were conducted to investigate the effects of pH and glucose concentrations on biological hydrogen production by using the natural sludge obtained from the bed of a local river as inoculant. Batch experiments numbered series I and II were designed at an initial and constant pH of 5.0–7.0 with 1.0 increment and four different glucose concentrations (5.0, 7.5, 10 and 20 g glucose/L). The results showed that the optimal condition for anaerobic fermentative hydrogen production is 7.5 g glucose/L and constant pH 6.0 with a maximum H2 production rate of 0.22 mol H2 mol−1 glucose h−1, a cumulative H2 yield of 1.83 mol H2 mol−1 glucose and a H2 percentage of 63 in biogas. 相似文献
12.
Gustavo Davila-Vazquez Ciria Berenice Cota-Navarro Luis Manuel Rosales-Colunga Antonio de León-Rodríguez Elías Razo-Flores 《International Journal of Hydrogen Energy》2009
Due to the renewed interest in finding sustainable fuels or energy carriers, biohydrogen (Bio-H2) from biomass is a promising alternative. Fermentative Bio-H2 production was studied in a continuous stirred tank reactor (CSTR) operated during 65.6 d with cheese whey (CW) as substrate. Three hydraulic retention times (HRTs) were tested (10, 6 and 4 h) and the highest volumetric hydrogen production rate (VHPR) was attained with HRT of 6 h. Therefore, four organic loading rates (OLRs) at a fixed HRT of 6 h were tested thereafter, being: 92.4, 115.5, 138.6 and 184.4 g lactose/L/d. The highest VHPR (46.61 mmol H2/L/h) and hydrogen molar yield (HMY) of 2.8 mol H2/mol lactose were found at an OLR of 138.6 g lactose/L/d; a sharp fall in VHPR occurred at an OLR of 184.4 g lactose/L/d. Butyric, propionic and acetic acids were the main soluble metabolites found, with butyric-to-acetic ratios ranging from 1.0 to 2.4. Bacterial community was identified by partial sequence analysis of the 16S rRNA and polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE). The results showed that at HRT of 10 h and 6 h were dominated by the Clostridium genus. The VHPR attained in this study is the highest reported value for a CSTR system using CW as substrate with anaerobic sludge as inoculum and represents a 33-fold increase compared to a previous study. Thus, it was demonstrated that continuous fermentative Bio-H2 production from CW can be significantly enhanced by an appropriate selection of parameters such as HRT and OLR. Enhancements in VHPR are significant because it is a critical parameter to determine the full-scale practical application of fermentation technologies that will be used for sustainable and clean energy generation. 相似文献
13.
Aruana Rocha Barros Eduardo Lucena Cavalcante de Amorim Cristiane Marques Reis Gessia Momoe Shida Edson Luiz Silva 《International Journal of Hydrogen Energy》2010
This study evaluated two different support materials (polystyrene and expanded clay) for biohydrogen production in an anaerobic fluidized bed reactor (AFBR) treating synthetic wastewater containing glucose (4000 mg L−1). The AFBRs contained either polystyrene (R1) or expanded clay (R2) as support materials were inoculated with thermally pre-treated anaerobic sludge and operated at a temperature of 30 °C and a pH of approximately 5.5. The AFBRs were operated with a range of hydraulic retention times (HRTs) between 1 and 8 h. For R1 with an HRT of 2 h, the maximum hydrogen yield (HY) was 1.90 mol H2 mol−1 glucose, with 0.805 mg of biomass (as total volatile solids, or TVS) attached to each g of polystyrene. For R2 operated at an HRT of 2 h, the maximum HY was 2.59 mol H2 mol−1 glucose, with 1.100 mg of attached biomass (as TVS) g−1 expanded clay. The highest hydrogen production rates (HPR) were 0.95 and 1.21 L h−1 L−1 for R1 and R2, respectively, using an HRT of 1 h. The H2 content increased from 16–47% for R1 and from 22–51% for R2. No methane was detected in the biogas produced throughout the period of AFBR operation. These results show that the values of HY, HPR, H2 content, and g of attached biomass g−1 support material were all higher for AFBRs containing expanded clay than for reactors containing polystyrene. 相似文献
14.
Shing-Der Chen Yung-Chung Lo Kuo-Shing Lee Tian-I. Huang Jo-Shu Chang 《International Journal of Hydrogen Energy》2009
Bio-hydrogen production from starch was carried out using a two-stage process combining thermophillic starch hydrolysis and dark H2 fermentation. In the first stage, starch was hydrolyzed by Caldimonas taiwanensis On1 using sequencing batch reactor (SBR). In the second stage, Clostridium butyricum CGS2 was used to produce H2 from hydrolyzed starch via continuous dark hydrogen fermentation. Starch hydrolysis with C. taiwanensis On1 was operated in SBR under pH 7.0 and 55 °C. With a 90% discharge volume, the reducing sugar (RS) production from SBR reactor reached 13.94 g RS/L, while the reducing sugar production rate and starch hydrolysis rate was 0.92 g RS/h/L and 1.86 g starch/h/L, respectively, which are higher than using other discharge volumes. For continuous H2 production with the starch hydrolysate, the highest H2 production rate and yield was 0.52 L/h/L and 13.2 mmol H2/g total sugar, respectively, under a hydraulic retention time (HRT) of 12 h. The best feeding nitrogen source (NH4HCO3) concentration was 2.62 g/L, attaining a good H2 production efficiency along with a low residual ammonia concentration (0.14 g/L), which would be favorable to follow-up photo H2 fermentation while using dark fermentation effluents as the substrate. 相似文献
15.
Batch and continuous tests were conducted to evaluate fermentative hydrogen production from starch (at a concentration of chemical oxygen demand (COD) 20 g/L) at 35 °C by a natural mixed culture of paper mill wastewater treatment sludge. The optimal initial cultivation pH (tested range 5–7) and substrate concentration (tested range 5–60-gCOD/L) were evaluated by batch reactors while the effects of hydraulic retention time (HRT) on hydrogen production, as expressed by hydrogen yield (HY) and hydrogen production rate (HPR), were evaluated by continuous tests. The experimental results indicate that the initial cultivation pH markedly affected HY, maximum HPR, liquid fermentation product concentration and distribution, butyrate/acetate concentration ratio and metabolic pathway. The optimal initial cultivation pH was 5.5 with peak values of HY 1.1 mol-H2/mol-hexose maximum HPR 10.4 mmol-H2/L/h and butyrate concentration 7700 mg-COD/L. In continuous hydrogen fermentation, the optimal HRT was 4 h with peak HY of 1.5 mol-H2/mol-hexose, peak HPR of 450 mmol-H2/L/d and lowest butyrate concentration of 3000 mg-COD/L. The HPR obtained was 280% higher than reported values. A shift in dominant hydrogen-producing microbial population along with HRT variation was observed with Clostridium butyricum, C. pasteurianum, Klebshilla pneumoniae, Streptococcus sp., and Pseudomonas sp. being present at efficient hydrogen production at the HRTs of 4–6 h. Strategies based on the experimental results for optimal hydrogen production from starch are proposed. 相似文献
16.
Xiao Wu Jun ZhuChunying Dong Curt MillerYecong Li Liang WangWanying Yao 《International Journal of Hydrogen Energy》2009
Liquid swine manure supplemented with glucose (10 g/L) was used as substrate for hydrogen production using an anaerobic sequencing batch reactor at 37 ± 1 °C and pH 5.0 under different hydraulic retention times (HRTs). Decreasing HRT from 24 to 8 h caused an increasing hydrogen production rate from 0.05 to 0.15 L/h/L. Production rates of both total biogas and hydrogen were linearly correlated to HRT with R2 being 0.993 and 0.997, respectively. The hydrogen yield ranged between 1.18 and 1.63 mol-H2/mol glucose and the 12 h HRT was preferred for high production rate and efficient yield. For all the five HRTs examined, the glucose utilization efficiency was over 98%. The biogas mainly consisted of carbon dioxide and hydrogen (up to 43%) with no methane detected throughout the experiment. Ethanol and organic acids were the major aqueous metabolites produced during fermentation, with acetic acid accounting for 56–58%. The hydrogen yield was found to be related to the acetate/butyrate ratio. 相似文献
17.
Kyung-Won Jung Dong-Hoon Kim Hang-Sik Shin 《International Journal of Hydrogen Energy》2010,35(24):13370-13378
The feasibility of continuous H2 production from coffee drink manufacturing wastewater (CDMW) was tested in two different types of reactors: a completely-stirred tank reactor (CSTR) and an up-flow anaerobic sludge blanket reactor (UASBr). While the performance in CSTR was limited, it was significantly enhanced in UASBr. The maximum H2 yield of 1.29 mol H2/mol hexoseadded was achieved at HRT of 6 h in UASBr operation. Non-hydrogenic, lactic acid was the dominant in CSTR, while butyric and caproic acids in UASBr. As caproic acid is generated by consuming acetic and butyric acids, all of which are related to H2 production, the presence of caproic acid in the broth also indicates H2 production, yielding 1.33 mol H2/glucose. It was speculated that the enhanced performance in UASBr was attributed to the high concentration of biomass over 60,000 mg VSS/L in the blanket zone, which provided insufficient substrate for indigenous lactic acid bacteria (LAB) to survive. The abundance of LAB in CDMW was confirmed by natural fermentation of CDMW. That is without the addition of external inoculum, CDMW was mainly fermented into lactic acid under mesophilic condition. For the first time ever, H2 producing granules (HPG) with diameters of 2.1 mm were successfully formed by using actual waste as a substrate. 相似文献
18.
Anaerobic hydrogen production from organic wastewater, an emerging biotechnology to generate clean energy resources from wastewater treatment, is critical for environmental and energy sustainability. In this study, hydrogen production, biomass growth and organic substrate degradation were comprehensively examined at different levels of two critical parameters (chemical oxygen demand (COD) and pH). Hydrogen yields had a reverse correlation with COD concentrations. The highest specific hydrogen yield (SHY) of 2.1 mole H2/mole glucose was achieved at the lowest COD of 1 g/L and decreased to 0.7 mole H2/mole glucose at the highest COD of 20 g/L. The pH of 5.5–6.0 was optimal for hydrogen production with the SHY of 1.6 mole H2/mole glucose, whereas the acidic pH (4.5) and neutral pH (6.0–7.0) lowered the hydrogen yields. Under all operational conditions, acetate and butyrate were the main components in the liquid fermentation products. Additionally, a comprehensive kinetic analysis of biomass growth, substrate degradation and hydrogen production was performed. The maximum rates of microbial growth (μm) and substrate utilization (Rsu) were 0.03 g biomass/g biomass/day and 0.25 g glucose/g biomass/day, respectively. The optimum pH for the rate of hydrogen production (RH2) and SHY were 5.89 and 5.74 respectively. Based on the kinetic analysis, the highest RH2 and SHY for batch-mode anaerobic hydrogen production systems were projected to be 13.7 mL/h and 2.32 mole H2/mole glucose. 相似文献
19.
An investigation of biological hydrogen production from glucose by Clostridium beijerinckii was conducted in a synthetic wastewater solution. A study examining the effect of initial pH (range 5.7–6.5) and substrate loading (range 1–3 g COD/L) on the specific conversion and hydrogen production rate has shown interaction behaviour between the two independent variables. Highest conversion of 10.3 mL H2/(g COD/L) was achieved at pH of 6.1 and glucose concentration of 3 g COD/L, whereas the highest production rate of 71 mL H2/(h L) was measured at pH 6.3 and substrate loading of 2.5 g COD/L. In general, there appears to be a strong trend of increasing hydrogen production rate with an increase in both substrate concentration and pH. Butyrate (14–63%), formate (10–45%) and ethanol (16–40%) were the main soluble products with other volatile fatty acids and alcohols present in smaller quantities. 相似文献
20.
Gopalakrishnan Kumar Chyi-How Lay Chen-Yeon Chu Jou-Hsien Wu Shih-Chi Lee Chiu-Yue Lin 《International Journal of Hydrogen Energy》2012
This study aimed to optimize the hydrogen production from various seed sludges (two kinds of sewage sludges (S1, S2), cow dung (S3), granular sludge (S4) and effluent from condensed soluble molasses H2 fermenter (S5)) and enhancement of hydrogen production via heat treatment for substrate and seed sludge by using the solid residues of biodiesel production (BDSR). Two batch assay tests were operated at a biodiesel solid residue concentration of 10 g/L, temperature of 55 °C and an initial cultivation pH of 8. The results showed that the peak hydrogen yield (HY) of 94.6 mL H2/g volatile solid (VS) (4.1 mmolH2/g VS) was obtained from S1 when substrate and seed sludge were both heat treated at 100 °C for 1 h. However, the peak hydrogen production rate (HPR) and specific hydrogen production rate (SHPR) of 1.48 L H2/L-d and 0.30 L H2/g VSS-d were obtained from S2 without any treatment. The heat treatment was found to increase the HY in both the cases of sewage sludges S1 and S2.The HY of 89.5 mL H2/g VS (without treatment) was increased to 94.6 mL H2/g VS and 82.6 mL H2/g VS (without treatment) was increased to 85.7 mL H2/g VS for S1 and S2. The soluble metabolic product (SMP) analysis showed that the fermentation followed mainly acetate–butyrate pathway with considerable production of ethanol. The total bioenergy production was calculated as 2.8 and 2.9 kJ/g VS for favorable hydrogen and ethanol production, respectively. The BDSR could be used as feedstock for dark fermentative hydrogen production. 相似文献