手性还原剂用于苯乙酮的不对称还原研究

（１Ｓ,２Ｒ）－１－二丁氨基－１,２－二氢－２－茚醇（ＲＯＨ）与氢化铝锂形成的手性试剂首次用于苯乙酮的不对称还原研究．根据手性试剂制备后的使用方法（方法Ａ∶立即使用;方法Ｂ∶回流１０ ｍ ｉｎ 后使用）,苯乙酮还原后可分别给出对映体过量值达６０％ ～７６％ 的不同主产物Ｒ－（＋ ）－１－苯基乙醇和Ｓ－（－）－１－苯基乙醇．研究了反应物物质的量之比（ＬｉＡｌＨ４ ｖＲＯＨｖＰｈＣＯＭｅ）对立体选择性的影响,当反应物物质的量之比为１．０ｖ１．５０ｖ１．５０时有较高的对映体过量值和转化率．这种手性还原剂为不对称合成光学活性醇提供了一种选择．     

食用香料1-辛烯-3-醇的合成

通过卤代戊烷的格氏试剂与丙烯醛加成制取松覃蘑菇中的主要香气成分１－辛烯－３－醇（松覃醇），讨论了反应条件如温度、反应时间、镁质量等对此反应的影响。     

牛磺酸的功能和合成新工艺研究

研究了牛磺酸（２－氨乙基磺酸）合成的新工艺：用氨基乙醇硫酸酯与亚硫酸钠溶液反应制备，收率达８５％。新工艺大大缩短了反应时间。     

新的光学增白剂

高质量的纸和特等纸板（卡片纸板）一个重要的技术和使用参数是白度，提高白度可能采用的较好方法之一是加入光学增白剂（OBA）。造纸工业用水溶性光学增白剂，基本要求是在抄纸过程的技术条件下它的作用稳定。应用在造纸工业的商业光学增白剂４．４一二氨基芪２．２一二磺酸，可用分子式Ⅰ表示如下：式中A１和A２是不同的芳香族或脂肪族胺和醇，A１和A２列于表１，OBANO２～４是新产品，是由４．４一二氨基区－２．２一二磺酸的酰化作用获得的。已知芪环中的烷氧基使得耐光和耐酸能力提高，有苯胺残渣改善OBA对纤维的亲和力，吗啉残…     

啤酒酵母抽提制品的研究

啤酒压榨酵母是我厂啤酒生产中的主要副产品之一，营养丰富。通过洗涤、除去杂质。用正交试验选择酵母酶解抽提的最佳条件；控制ＰＨ６．０－７．０，温度５０－６０℃，盐１－１．２％，酶制剂０．５－１．０‰，自溶酶解８－１２小时，酵母氨基Ｎ抽出率增加一倍以上，达到先进的水平。酶解酵母抽出物，经离心后分别制成液膏状三种产品，液状产品含氨基，Ｎ１．５，膏状产品含氨基Ｎ２．５，粉状产品含蛋白质４０－５％，游离氨基酸     

酶法水解鲜牛骨骼的研究

以新鲜牛骨为原料，用国产胰酶进行酶水解反应，适宜反应条件为：骨水＝１：２，骨：酶＝１０００：１－２０００：１，控制反应温度４８－５０℃ｐＨ＝９，反应时间６－８ｈ，蛋白质提取率≥７０％。     

牛磺酸的功能和合成新工艺研究

研究了牛磺酸（２－氨乙基磺酸）合成的新工艺：用氨基乙醇硫酸酯与亚硫酸钠溶液反应制备,收率达８５％。新工艺大大缩短了反应时间。     

DOX型改性恶唑烷合成鞣剂研究

ＤＯＸ型改性恶唑烷合成鞣剂研究周风文（西北轻工业学院）ＤＯＸ型改性恶唑烷鞣剂是恶唑烷（ＯＸ－２）与丙烯酸树脂的混配物，其合成工艺为：恶唑烷（ＯＸ－２）的缩合：在５００升釜中加入一定比例β－氨基醇，在搅拌下滴加一定量的甲醛，滴加反应完全后，静置过滤，次...     

杂多酸催化合成马来酸(2-2乙基己基)酯的研究

杂多酸（ＨＷＰ）为催化剂,以顺丁烯二酸酐与仲辛醇为原料催化合成马来酸（２－２乙基己基）酯。研究了催化剂的用量、酯化、磺化反应时间、酸醇摩尔比对反应的影响,催化剂能使用多次催化效果较好。     

匀染剂2—氨基丁醇硫酸钠的合成与应用

本文以２－氨基丁醇为原料合成匀染剂２－氨基丁醇硫酸钠，该匀剂适用于酸性染料染羊毛，丝绸，等，     

Purification and characterization of vanillyl-alcohol oxidase from Byssochlamys fulva V107

Vanillyl-alcohol oxidase from Byssochlamys fulva V107 was purified to apparent homogeneity as shown by SDS-PAGE and gel-permeation HPLC. The enzyme is a homodimeric flavoenzyme consisting of two 58 kDa subunits. It catalyzes the dehydrogenation of different 4-hydroxybenzylic structures, including the conversion of 4-hydroxybenzyl alcohols such as vanillyl alcohol to the corresponding aldehydes, eugenol to coniferyl alcohol, and 4-alkylphenols to 1-(4-hydroxyphenyl)alcohols. The latter reaction was S-stereospecific and was used for the synthesis of S-1-(4-hydroxyphenyl)ethanol and -propanol with enantiomeric excesses of 81.9 and 86.0%, respectively. The catalytic and structural similarities to a Penicillium vanillyl-alcohol oxidase and Pseudomonas 4-alkylphenol methylhydroxylases are discussed.     

Asymmetric direduction of 1,2-indanedione to cis (1S,2R) indanediol by Trichosporon cutaneum MY 1506

Cis (1S,2R) indanediol is a potential precursor to (-)-cis (1S,2R)-1-aminoindan-2-ol, a key chiral synthon for a leading HIV protease inhibitor, Crixivan (Indinavir). A potential route to the biosynthesis of this important precursor, the microbial asymmetric direduction of 1,2-indanedione to its corresponding diol, cis (1S,2R) indanediol, was investigated. The screening of 32 yeast strains yielded Trichosporon cutaneum MY 1506 as a suitable biocatalyst. At the 2-l shake-flask scale, 1,2-indanedione (charged at 1.0 g/l) was bioconverted to cis (1S,2R) indanediol at a final bioconversion yield of 99.1% and an enantiomeric excess of >99%. When scaled up in a 23-l bioreactor, T. cutaneum produced 8.4 g of pure cis (1S,2R) indanediol, and the isolated yield of cis (1S,2R) indanediol was 52%. Purification of the scale-up also yielded 0.9 g of the more polar trans (1S,2R) indanediol diastereomer, a minor bioreduction product. Supercritical fluid chromatography analyses of the purified cis (1S,2R) and trans (1S,2S) indanediol demonstrated that the enantiomeric excesses during this bioconversion scale-up were 99% and 26%, respectively.     

Optimization of lipase-catalyzed enantioselective esterification of (±)-menthol in ionic liquid

Response surface methodology was successfully applied to optimize lipase-catalyzed enantioselective esterification of (±)-menthol. The effects of various reaction conditions, including reaction time, temperature, enzyme loading, substrate molar ratio and water activity, were investigated. A Central Composite Rotatable Design was employed to search for the optimal conversion of (±)-menthol and enantiomeric excess. A quadratic polynomial regression model was used to analyze the experimental data at a 95% confidence level (p < 0.05). The analysis confirmed that reaction temperature, enzyme loading and reaction time were the significant factors affecting the conversion of (±)-menthol. Moreover, reaction temperature, enzyme loading, substrate molar ratio and reaction time were found to affect the enantiomeric excess significantly. The coefficient of determination of these two models was found to be 0.980 and 0.967, respectively. Two sets of optimum reaction conditions were established and the verified experimental trials were performed for validating the optimum points. Under the optimum conditions, the conversion of (±)-menthol and the enantiomeric ratio exceeded 53% and 40%, respectively.     

Synthesis of (-)-β-caryophyllene oxide via regio- and stereoselective endocyclic epoxidation of β-caryophyllene with Nemania aenea SF 10099-1 in a liquid-liquid interface bioreactor (L-L IBR)

Nemania aenea SF 10099-1, a basidiomycete isolated from a forest soil sample, regio- and stereoselectively epoxidized β-caryophyllene (Car) to (−)-β-caryophyllene oxide (Car-Ox) in a liquid–liquid interface bioreactor (L–L IBR) consisted of a liquid medium (a bottom phase), a fungus-ballooned microsphere (MS) mat (a middle phase), and an organic phase containing Car (a top phase). The cultivation conditions, such as carbon and nitrogen sources, kind of MS, initial medium pH and Car concentration, were optimized in the L–L IBR system. The best carbon and nitrogen sources were xylose and tryptone, respectively. The most suitable polyacrylonitrile MS was MMF-DE-1 (former MFL-80SDE; non-coated type). Although the strain could not grow below pH 5.5, the endocyclic epoxidation of Car efficiently proceeded at a wide range of initial medium pH (6.0 to 9.0). The bioconversion system exhibited an excellent alleviation effect toward substrate and product inhibitions. While Car could be added into an organic phase (KF-96L-1CS, dimethyl silicone oil) at 50% (w/v), the accumulation of Car-Ox reached over 30 g/l in spite of these strong microbial toxicities. Moreover, the epoxidation reaction smoothly proceeded in a novel L–L IBR system, a multistory L–L IBR systems, consisted of 5 stacked reactor units. The optical rotation of Car-Ox produced was (−) and the enantiomeric excesses of (−)-β-Car-Ox purified by 1st and 2nd recrystallization from methanol reached 97.51 and 99.33%, respectively.     

Accumulation of ectoine in the halotolerant Brevibacterium sp. JCM 6894

Resting cells of Fusarium moniliforme strain MS31 produced (R)-1-phenylpropanol from propylbenzene. The components of the medium and the reaction conditions were adjusted to increase the specific activity of the hydroxylating enzyme involved. Glucose and sodium nitrate were selected as carbon and nitrogen sources, respectively. The substrate, propylbenzene, inhibited fungal growth and the activity of the enzyme. Acetoin added to the medium increased both growth and activity of the enzyme, and hydroxylation of propylbenzene increased by 1.4-fold. Maximum bioconversion of propylbenzene by resting cells of the fungus was at 25-30 degrees C and pH 7.0 with cells at concentration of 40 mg (dry) per milliliter of reaction mixture. Conversion was accelerated as soon as propylbenzene was added; slowing 2 h later. In the end, F. moniliforme strain MS31 produced (R)-1-phenylpropanol with an enantiomeric excess of 98% at the concentration of 16 mM (2.2 mg.ml(-1)).     

Kinetic resolution of an indan derivative using Bacillus sp. SUI-12: synthesis of a key intermediate of the melatonin receptor agonist TAK-375

The chiral indan derivative (S)-2 (2-[(8S)-1,6,7,8-tetrahydro-2H-indeno[5,4-b]furan-8-yl]ethyl-amine) was synthesized by enzyme-catalyzed asymmetric hydrolysis of the racemic acetamide 1 (N-[2-(1,6,7,8-tetrahydro-2H-indeno[5,4-b]furan-8-yl)ethyl]acetamide). The reaction was carried out using Bacillus sp. SUI-12 screened for the ability to hydrolyze 1 to give (S)-2 with high enantioselectivity. In a scaled-up experiment, a low reaction rate was observed. However, by changing the culture medium and the reaction conditions, it became possible to run the reaction to 40% conversion on a 10-g or more scale, obtaining (S)-2 at >;99% enantiomeric excess (ee). The (S)-2 obtained was available for the synthesis of the melatonin receptor agonist TAK-375 (N-[2-[(8S)-1,6,7,8-tetrahydro-2H-indeno[5,4-b]furan-8-yl]ethyl]propanamide).     

Practical production of (S)-1,2-propanediol and its derivative through baker's yeast-mediated reduction

The enantiomeric excess (ee) of (S)-1,2-propanediol produced by baker's yeast-mediated reduction of 1-acetoxy-2-propanone was improved to 96% ee by a fed-batch operation of the substrate. A similar reduction of 1-benzoyloxy-2-propanone stopped because of the inhibition toward the enzymes for NADPH regeneration by the reduction product. The inhibition was prevented using resin that adsorbs the product from the reaction mixture, and 70 g/l substrate was reduced yielding (S)-1-benzoyloxy-2-propanol at > 99% ee.     

Influence of Stage of Ripeness on the Enantiomeric Distribution of Chiral Terpenes in Blackcurrant Fruits (Ribes nigrum L.

ABSTRACT: Changes in the enantiomeric composition of chiral terpenes during ripening were studied in 3 different blackcurrant ( Ribes nigrum L.) varieties (Hedda, Baldwin, and Ben Hope) by solid-phase microextraction-gas chromatography (SPME-GC). The enantiomeric distribution of some terpenes remained constant, whereas others (β-pinene, limonene, and α-phellandrene) exhibited considerable variation. These data are indicative of the occurrence of 2 new biochemical pathways reported in blackcurrant for the first time. The overall level of chiral and non-chiral terpenes was examined at different stages of ripening. A slight increase in alcohol terpenes and a substantial drop in monoterpene and sesquiterpene hydrocarbons was found in the latter stages.     

Stereoisomeric flavour compounds

The enantiomeric distribution of 3-mercaptohexanol (1) and 3-methylthiohexanol (2) in yellow and purple passion fruits was determined by capillary gas chromatography (GC) using two different chiral stationary phases and a flame photometric detector. The results were confirmed by enantioselective capillary GC combined with mass spectrometric detection.1 shows an enrichment of the (S)-enantiomer, but the enantiomeric purity varies in a wide range. Irrespective from the enantiomeric distribution of1, 2 was detected with high enantiomeric purity in favour of the (S)-enantiomer. Using the method presented the addition of synthetic, racemic2 is easily detected.     

Penicilium expansum PED-03固态发酵产脂肪酶及酶学性质研究

利用Penicilium expansum PED03固态发酵产脂肪酶,选用麸皮为碳源、豆粕为氮源,m(麸皮)/m(豆粕)比为1∶4,适宜含水量为50%(V/W),在24℃下发酵4d,脂肪酶活力可达1596U/g。该酶的最适温度为35℃,在50℃以下较为稳定;最适pH值为9.5,在pH6.0～10.0范围内有明显的催化活性;适宜浓度的Na+、Ca+2、Mg+2对酶反应有促进作用,而Cu2+、Fe2+和Mn2+等对酶反应有不同程度的抑制作用。利用P.expansumPED03脂肪酶在非水相中对外消旋烯丙醇酮(4羟基3甲基2(2烯丙基)2环戊烯1酮,allethrolone)进行酶法拆分,35℃反应36h时转化率(C)可达理论值的96%,产物的对映体过量值(eep)可达99%,显示了良好的应用潜力。