Responsiveness of ‘Conference’ pears to 1‐methylcyclopropene: the role of harvest date,orchard location and year

BACKGROUND: In some pear varieties like ‘Conference’, 1‐methylcyclopropene (1‐MCP) treatment often impairs the ripening process indefinitely and the pears remain ‘evergreen’. To better understand this behaviour, the influence of the harvest date, orchard location and year on the effectiveness of 1‐MCP treatment was investigated. RESULTS: Pear softening was inhibited by 1‐MCP treatment and the effectiveness of the treatment depended on harvest date, orchard location and year. Differences in the rate of softening in 1‐MCP‐treated pears depended mainly on the fruit physiological maturity at the moment of 1‐MCP treatment. Accordingly, the combination of the Streif index and ethylene production at harvest appeared to be able to predict the evergreen behaviour. Treated pears with a low Streif index (<0.8) and high ethylene production at harvest (≥0.23 µL kg^?1 h^?1) maintain significantly high firmness but did soften during shelf life, reaching acceptable eating quality. CONCLUSION: Evergreen behaviour was mainly influenced by the initial fruit maturity and especially by the ability of the fruit to produce ethylene at the moment of treatment. More mature fruit were able to overcome the inhibition by 1‐MCP, and the solution to prevent evergreen behaviour therefore lies in the adequate determination of harvest maturity. © 2012 Society of Chemical Industry     

Polyphenoloxidase activity and total phenolic content as related to browning of minimally processed ‘Jonagored’ apple

Phenolic content, polyphenoloxidase (PPO) activity and browning have been reported by different authors to vary in different ways, depending on cultivar, picking maturity, storage conditions, etc. Numerous authors have attempted to correlate browning results with the phenolic content and/or enzymatic activity of apples, but data published in the literature are divergent as to which of the two factors, ie the enzyme or the substrate, plays the decisive role in the overall phenomenon of enzymatic browning. The aim of this work was to follow the PPO activity and phenolic content of minimally processed apple (cv ‘Jonagored’) during cold storage and to establish a potential relationship with enzymatic browning. Increasing storage time of minimally processed apple seemed to result in an increase in PPO activity. No changes (P > 0.05) in total phenolic content were observed during storage. Colour degradation of apple cubes, as indicated by changes in colour parameters, was found to be highly correlated with PPO activity. Browning of ‘Jonagored’ apple cubes during storage was found to be moderately correlated with PPO activity. No correlation was found between the browning index and the total phenolic content. © 2001 Society of Chemical Industry     

Chilling injury development of ‘Tahitian’ lime, ‘Emperor’ mandarin, ‘Marsh’ grapefruit and ‘Valencia’ orange

The effect of varying levels of ethylene on the chilling injury (CI) development and the changes in the levels of putrescine, squalene and α-farnesene of ‘Tahitian’ lime (Citrus latifolia Tanaka), ‘Emperor’ mandarin (Citrus reticulata Blanco), ‘Marsh’ grapefruit (Citrus paradisi Macf) and ‘Valencia’ orange (Citrus sinensis L Osbeck) stored at 0°C was investigated. It was found that different citrus fruits stored at 0°C had varying sensitivity to CI, and that low levels of exogenous ethyiene induced earlier and more severe CI in all citrus fiuits. The levels of endogenous putrescine, squalene and α-farnesene varied between fruit, and was affected by the time of exposure at 0°C and the presence of ethylene. The patterns of change indicate that loss of squalene coupled with loss of α-farnesene could be involved in induction of CI.     

Recent developments in cold plasma-based enzyme activity (browning,cell wall degradation,and antioxidant) in fruits and vegetables

According to the Food and Agriculture Organization of United Nations reports, approximately half of the total harvested fruits and vegetables vanish before they reach the end consumer due to their perishable nature. Enzymatic browning is one of the most common problems faced by fruit and vegetable processing. The perishability of fruits and vegetables is contributed by the various browning enzymes (polyphenol oxidase, peroxidase, and phenylalanine ammonia-lyase) and ripening or cell wall degrading enzyme (pectin methyl-esterase). In contrast, antioxidant enzymes (superoxide dismutase and catalase) assist in reversing the damage caused by reactive oxygen species or free radicals. The cold plasma technique has emerged as a novel, economic, and environmentally friendly approach that reduces the expression of ripening and browning enzymes while increasing the activity of antioxidant enzymes; microorganisms are significantly inhibited, therefore improving the shelf life of fruits and vegetables. This review narrates the mechanism and principle involved in the use of cold plasma technique as a nonthermal agent and its application in impeding the activity of browning and ripening enzymes and increasing the expression of antioxidant enzymes for improving the shelf life and quality of fresh fruits and vegetables and preventing spoilage and pathogenic germs from growing. An overview of hurdles and sustainability advantages of cold plasma technology is presented.     

Pre‐ or post‐harvest applications of putrescine and low temperature storage affect fruit ripening and quality of ‘Angelino’ plum

BACKGROUND: Plum has a very short storage life. The role of pre‐ or post‐harvest applications of putrescine (PUT) and low temperature storage on fruit ripening and quality was investigated in plum fruit (Prunus salicina Lindl. cv. Angelino). RESULTS: Pre‐ or post‐harvest PUT treatments [(0.1, 1.0 or 2.0 mmol L^?1) + 0.01% Tween‐20 as a surfactant] delayed and suppressed the climacteric ethylene production and respiration rate irrespective of the method used to apply PUT. PUT‐treated fruit following low temperature storage (0 ± 1 °C; 90 ± 5% RH), at the ripe stage exhibited higher fruit firmness and titratable acidity (TA), while soluble solids content (SSC), levels of ascorbic acid, total carotenoids and total antioxidants were lower than in untreated fruit. Fruit both sprayed with PUT and stored in low temperature for 6 weeks, at the ripe stage showed reduced respiration rate, delayed changes in the SSC:TA ratio and levels of total carotenoids compared to post‐harvest PUT application. CONCLUSION: Pre‐harvest application of 2.0 mmol L^?1 PUT 1 week before the anticipated commercial harvest was more effective in delaying plum fruit ripening and can be used to extend the storage (0 ± 1 °C) life of plums for up to 6 weeks with minimum losses in fruit quality. Copyright © 2008 Society of Chemical Industry     

Automatic sorting of Chinese jujube (Zizyphus jujuba Mill. cv. ‘hongxing’) using chlorophyll fluorescence and support vector machine

Fruit classification is important to improve quality during processing, storage and marketing. The aim of the study was to determine if a new system combining chlorophyll fluorescence (ChlF) and C-support vector machine (C-SVM) might assist the classification of jujube fruits based on postharvest quality, including ascorbic acid and total phenols contents and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity. Our results showed that the best classification accuracy of fruit quality was up to 93.33% using the RBF SVM classifier (C = 2, γ = 0.5), and the correct classification rates of 86.67% was achieved for the sigmoid (C = 2, γ = 0.5) SVM classifier as well as the polynomial (C = 2, γ = 0.5, d = 1) SVM classifier. The proposed SVM classifier achieved the best classification accuracy, showing that the SVM-ChlF system can provide a potential tool for automatically classifying the quality of not only jujube fruits, but also any other chlorophyll-containing fruits in packing lines.     

Effect of ethylene and 1‐MCP treatments on strawberry fruit ripening

BACKGROUND: Strawberry is a soft fruit, considered as non‐climacteric, being auxins the main hormones that regulate the ripening process. The role of ethylene in strawberry ripening is currently unclear and several studies have considered a revision of the possible role of this hormone. RESULTS: Strawberry fruit were harvested at the white stage and treated with ethephon, an ethylene‐releasing reagent, or 1‐methylcyclopropene (1‐MCP), a competitive inhibitor of ethylene action. The effects of the treatments on fruit quality parameters and on the activity of enzymes related to anthocyanin synthesis and cell wall degradation were evaluated. Some aspects of ripening were accelerated (anthocyanin accumulation, total sugar content and increment of phenylalanine ammonia‐lyase (PAL; EC 4.3.1.24) and β‐galactosidase (EC 3.2.1.23) activities), while others were repressed (chlorophyll levels and increment of endo‐1,4‐β‐glucanase (EC 3.2.1.4) and β‐xylosidase (EC 3.2.1.37) activities) or unchanged (reducing sugar content, pH, titratable acidity and α‐L ‐arabinofuranosidase (EC 3.2.1.55) activity) by ethylene. 1‐MCP treatment caused the opposite effect. However, its effects were more pronounced, particularly in anthocyanin accumulation, phenolics, PAL and polygalacturonase (EC 3.2.1.15 and EC 3.2.1.67) activities. CONCLUSION: These observations probably indicate that strawberry produces low levels of ethylene that are sufficient to regulate some ripening aspects. Copyright © 2010 Society of Chemical Industry     

Enhancing disease resistance in harvested mango (Mangifera indica L. cv. ‘Matisu’) fruit by salicylic acid

To learn how salicylic acid (SA) may affect disease resistance in mango, mango fruit (Mangifera indica L. cv. ‘Matisu’) were treated with 1 mmol L^?1 SA solution under vacuum infiltration for 2 min at a low pressure (?80 kPa) and for an additional 10 min at air pressure. The fruit were inoculated with anthracnose (Colletotichum gloeosporioides Penz.) spore suspension (1 × 10⁴ CFU mL^?1) and incubated at 13 °C, 85–95% RH. Disease incidence and lesion diameter in/on the SA‐treated fruit were 37.5% and 20.9% lower than that in/on control fruit on the 4th day of incubation. The study further showed that activities of defensive enzymes in the fruit were significantly enhanced by SA treatment. The activity of phenylalanine ammonia‐lyase (PAL) and β‐1,3‐glucanase in the SA‐treated fruit was over 6‐ or 0.9‐fold higher than that in control fruit on the 4th day after the fruit being treated with SA, respectively. Level of hydrogen peroxide (H₂O₂) or superoxide radicals (O₂^?) generation rate in SA‐treated fruit was 22.3% or 79.4% higher than that in controls on the 8th day after the fruit being treated with SA, respectively. These results suggested that PAL and β‐1,3‐glucanase, as well as H₂O₂ or O₂^?, may be involved in the enhancement of disease resistance in mango fruit. Copyright © 2006 Society of Chemical Industry     

Effect of pre‐ripening chilling temperatures on ripening,shelf life and quality of bananas treated with 1‐methylcyclopropene

Ethylene production, shelf life and fruit quality of Cavendish bananas (cv. Williams), held at different pre‐ripening storage temperatures (5 °C, 10 °C or 15 °C) prior to ethylene and/or 1‐methylcyclopropene (1‐MCP) application, were determined and compared. Levels of ethylene production during ripening were highest for control fruit that had been stored at 5 °C followed by 10 °C and then 15 °C. 1‐MCP treatment (at 300 nL L^?1) had no effect on ethylene production of fruit that had been stored at 10 °C by day 10 of storage, but ethylene production was significantly higher for fruit that had been stored at 15 °C and lower for those stored at 5 °C before 1‐MCP treatment. 1‐MCP also increased shelf life to a greater extent when applied to fruit that had been stored at 10 °C or 15 °C. Even though 1‐MCP had no significant effect on under peel chilling injury, it increased discolouration significantly, regardless of storage temperature. Firmness of 1‐MCP‐treated fruit decreased significantly compared to the control when fruit were held at 5 °C or 15 °C prior to ripening (and 1‐MCP application) but had no effect on fruit stored at 10 °C before ripening. These results indicate that pre‐ripening storage temperature affects ripening, shelf life and quality in both ethylene and 1‐MCP‐treated bananas.     

Relationship between soil temperature and fruit colour development of ‘Clemenpons’ Clementine mandarin (Citrus clementina Hort ex. Tan)

BACKGROUND: In Citrus, root temperature regulates rind colouration. However, few studies have investigated the range of temperatures and timing which determine rind colour break. The objective of this study was to determine the relationship between range of soil temperature (ST) and rind colour development in the precocious ‘Clemenpons’ Clementine mandarin. Reflective white plastic mulch was used to modify root temperature. RESULTS: Mulching increased reflected light and reduced daily maximum ST and temperature range, major differences being established 70–30 days before harvest. Rind colour‐break correlated positively with 20 °C < ST < 23 °C; thus, 20–23 °C appears to be the ST threshold interval for fruit colouration. The sooner the soil reached it, the sooner the fruit changed rind colour. In our experiments, control trees accumulated 565 h at this ST interval before fruit changed colour, whereas in treated trees it occurred 2 weeks earlier. Hence, in treated trees the colour break was advanced by 2 weeks and this increased the percentage of fruit harvested at the first picking date by up to 2.5‐fold. Conclusions: Fruit colour‐break does not take place at a certain ST, but after several hours at a ST of 20–23 °C. In our experiments, reducing ST during the 2 months before harvest advances the first picking date in the ‘Clemenpons’ Clementine mandarin. Copyright © 2011 Society of Chemical Industry     

Extending the shelf‐life of bananas with 1‐methylcyclopropene and a chitosan‐based edible coating

BACKGROUND: The marketability of bananas over long distances has been limited due to their highly perishable nature and sensitivity to ethylene. To increase the shelf‐life several alternatives have been tested in attempts to maintain quality characteristics. We evaluated the effects of 1‐methylcyclopropene (SmartFresh^SM, 1‐MCP) and a chitosan‐based edible coating (FreshSeal^®, EC), applied alone or combined, on bananas at ripeness stage 3. All fruits were stored for 8 days at 22 °C, 85% RH. Color development, weight loss, firmness, physiological, sensory and chemical variables were evaluated. RESULTS: After 3 days, control and EC‐treated fruits were completely yellow (°Hue = 90), while 1‐MCP treated fruits alone and combined with EC were still showing some green colorations on tips and neck of fingers. After 7 days, fruits treated with the combination (EC + 1‐MCP) reached a Hue value of 90. Firmer fruits were observed with 1‐MCP alone or combined with EC. A decrease of 0.15% titratable acidity and an increase of 8°Brix were observed in all treatments during the experiment. The incidence of sugar spots was delayed and diminished on fruits treated with 1‐MCP either alone or combined. Sensory results showed no adverse effects by using the 1‐MCP or the edible coating. CONCLUSION: The combined treatment of EC + 1‐MCP could be used to extend the commercial life of bananas for up to four more days. Copyright © 2009 Society of Chemical Industry     

‘Diffuse skin browning’ in 1‐MCP‐treated apples: etiology and systems of control

BACKGROUND: ‘Diffuse skin browning’ (DSB) is a physiological disorder that affects Golden Delicious apples treated with 1‐methylcyclopropene (1‐MCP). Although a very high incidence is found, very little is known about the etiology of this disorder. This study aims to provide an understanding of the causes of this disorder and prevent it. RESULTS: A very high incidence of DSB was found in 1‐MCP‐treated apples independent of the location of the orchard. Similar to superficial scald, harvest maturity determines the DSB incidence, with the more mature fruit being less sensitive. The 1‐MCP dose (156 nL L^?1 or 625 nL L^?1) and the temperature at which the 1‐MCP treatment was applied (0.5 or 20 °C) did not affect the incidence of DSB. Diphenylamine (DPA) treatment did not prevent DSB, contrary to superficial scald. Additionally, controlled atmosphere storage only partially reduced the incidence of DSB, whereas progressive cooling strategies completely inhibited DSB occurrence. A direct correlation was found between the sensitivity of the Golden Delicious clone to russeting and its sensitivity to develop DSB during storage. CONCLUSION: Our results indicated that DSB and superficial scald are two different disorders involving different oxidative processes. DSB can be prevented by progressive cooling and selection of russeting‐resistant clones. Copyright © 2010 Society of Chemical Industry     

Monitoring colour,volatiles in the headspace and enzyme activity to assess the quality of broccoli florets (Brassica oleracea L. italica cv. Bellstar and Legacy) during postharvest storage

The research objective was to study whether volatile organic compounds released in the headspace could be used to assess the broccoli quality (Brassica oleracea L. italica cv. Bellstar and Legacy) during postharvest storage (23 °C for 7 days) and correlated with colour change and the activity of peroxidase/POD, ascorbic acid oxidase/AAO and lipoxygenase/LOX. Volatile organic compounds were monitored using PTR‐MS. Methanol had the highest concentration compared with other volatiles released in the headspace that significantly increased during senescence. The methanol concentration was approximately 500 ppbv before the broccoli colour began to change from green to yellow. The concentration of methanol in the headspace increased to 1000–1100 ppbv for Bellstar and Legacy when the colour became yellowish. LOX had high correlation (0.91 and 0.83 for Bellstar and Legacy, respectively) with volatiles for both cultivars, but not for POD. Methanol production is proposed as the best volatile marker to assess the broccoli quality.     

Effect of cold‐shock treatment on chilling injury in mango (Mangifera indica L. cv. ‘Wacheng’) fruit

To investigate how cold shock may affect chilling injury in mango fruit (Mangifera indica L. cv. ‘Wacheng’), the fruit were treated for 3, 4 or 5 h at 0 °C, or treated for 8, 10 or 12 h at 4 °C, respectively, then transferred to 20 °C for 20 h prior to being stored at 2 °C, 85–95% RH. The chilling injury index of mango treated at 0 °C for 4 h was 59.7% lower than that of the control fruit directly stored at 2 °C, 85–95% RH. Some attributes were assayed in the fruit treated at 0 °C for 4 h. Ion leakage of the cold‐shock fruit at 0 °C for 4 h was 16% or 10% lower than that of the control on day 9 or day 12 of storage, respectively. Malondialdehyde content of the cold‐shocked fruit was 70% or 50% lower than that of the control on day 6 or day 12 of storage at 2 °C, respectively. Activities of catalase, ascorbate peroxidase, and contents of glutathione and phenolic compounds in the fruit during storage were all markedly enhanced by the cold‐shock treatment, whereas activities of superoxide dismutase, glutathione reductase and content of ascorbic acid in the mango were slightly influenced by the cold‐shock treatment. These results suggest that cold‐shock treatment may hold promise as an alternative approach to reduce chilling injury in mango fruit during cold storage. Copyright © 2006 Society of Chemical Industry     

Sensory quality of ethylene‐exposed carrots (Daucus carota L,cv ‘Yukon’) related to the contents of 6‐methoxymellein,terpenes and sugars

Carrots were analysed for taste and odour and for contents of terpenes, 6‐methoxymellein and sugars during 3 weeks storage at 15 °C in an atmosphere containing ethylene (1 µl l⁻¹). The ethylene treatment caused an increase in 6‐methoxymellein and the conversion of higher amounts of sucrose to fructose and glucose compared to control carrots stored in air. This corresponded to higher sensory scores for bitterness and terpene flavour and a lower score for sweetness, as measured by an expert taste panel. Principal component analysis showed a more expressed bitter taste, earthy flavour, green flavour, terpene flavour and aftertaste in the ethylene‐treated carrots. Correlations were found between sweet taste and the content of sucrose (r = 0.91, p < 0.005) and between the contents of various terpenes (particularly γ‐terpinene, limonene and caryophyllene) and terpene flavour, green flavour, aftertaste and bitter taste (r ≥ 0.72, p < 0.05). In the air‐stored carrots these off‐flavours seemed to be masked by a high sucrose content. © 2000 Society of Chemical Industry