基于微透镜阵列的多通道光寻址电位传感器研

为了提高化学和生物图像传感器的成像速度和简化成像系统,提出了一种基于微透镜阵列的多通道光寻址电位传感器.利用微透镜阵列把单束激光转化成点阵,通过多频率斩波器,得到不同频率的光点阵列用作光寻址电位传感器的光源,从而实现多点同时检测.实验结果表明该系统具有良好的频域分辨率,通过数字补偿技术可获得各个检测点的电流电压特性曲线,证明可实现多点同时检测.该系统可以用于光寻址电位传感器和扫描光感阻抗传感器的快速成像,以及多通道化学生物图像检测.     

光寻址电位传感器的自相关检测方法

针对基于光寻址电位传感器的微流控芯片信噪比低的问题,提出了一种基于自相关检测的光寻址电位传感器信号提取方法。采用气液分离的方法在微流路内产生了体积约1μL的样品微滴,对比了光寻址电位传感器在方波激励与正弦波激励下检测微滴的I/V特性曲线,以及在相同条件下的I-f特性曲线。结果表明：采用自相关检测法,光寻址电位传感器具有53.8 mV/pH的灵敏度,其信噪比提高了7.020 6 dB。研究结果对于微型LAPS光电流信号高精度检测做出了有益探索,具有良好的应用前景。     

碳纤维温度传感器的感温机理及其应用研究

针对连续流光寻址电位传感器微分析系统样品溶液消耗量大的问题,本文提出了一种液滴型光寻址电位传感器微流控芯片系统。该液滴芯片以电渗微泵驱动微流体,通过电渗流动将样品溶液引流至微流路内,形成单个样品液滴。研究了样品液滴I/t特性曲线,当样品液滴流经检测点时,光电流突然升高至0.1 μA左右,当样品液滴流出检测点时,光电流降至最小。研究了不同pH值、体积均为1 μL样品液滴的I/V特性曲线,得出光寻址电位传感器的灵敏度为45.9 mV/pH;其它条件不变,改变微流路深度,获得不同pH值体积均为200 nL的样品液滴的I/V特性曲线,得出光寻址电位传感器的灵敏度为47.5 mV/pH。结果表明,可以通过减小流路深度将所需测试样品的体积减少至200 nL。该液滴型光寻址电位传感器微分析系统有望应用于微量化学/生物样品的分析。     

集成多传感器的环境监测电子舌的研究

研发了一种基于硅基底的环境监测新型电子舌,用于在线检测锌、镉、铅、铜、铁和铬6种痕量重金属元素.系统由一个电化学微电极阵列和两个光寻址电位传感器组成.其中微电极阵列由8×8个直径为10 μm的金微电极组成,采用方波阳极溶出伏安法实现锌、镉、铅和铜4种元素的快速检测.另外在硅基底的两个2 mm×2 mm的金膜表面,分别沉积铁离子和铬离子选择性薄膜,实现光寻址电位传感器,用来实时检测铁和铬2种元素.实验结果表明,本文方法具有所需样品少、操作方便、检测速度快以及灵敏度高等优点,检出限可达到微克/升级.     

光寻址电位传感器及其应用

光寻址电位传感器(LAPS)是一种基于半导体光电效应的化学传感器,在稳定性、重复性、灵敏度、准确性和响应速度等方面具有优良的性能。介绍了LAPS的基本原理和测量方法,阐述了LAPS在细胞传感器、图像传感器、金属离子敏感薄膜传感器、气体传感器及生物医学领域中的应用,并对其存在问题和研究方向进行了总结和展望。     

多孔硅气体传感器

介绍了多孔硅气体传感器的原理和优点,综述了多孔硅气体传感器测量氮氧化物和几种有机气体的机理、方法及最新的研究进展,并分析了传感器的选择性和稳定性,展望了多孔硅气体传感器应用前景。     

氧化钨纳米线修饰多孔硅结构的制备及NO2气敏性能研究

针对多孔硅气敏传感器在室温下对NO2气体灵敏度较低、选择性不强的问题,采用双槽电化学腐蚀法制备多孔硅,然后在多孔硅顶部溅射沉积金属钨薄膜并经高温热处理氧化形成WO3纳米线,制备出WO3纳米线修饰多孔硅结构及其气敏传感器,对WO3纳米线/多孔硅材料进行了SEM和XRD分析,测试了传感器室温下对NO2的气敏特性。结果表明,制备WO3纳米线的最佳热处理条件是700℃,此温度下增加金属钨膜溅射时间可提升WO3纳米线的生长密度? 所制备的传感器对NO2气体表现出反型气敏响应,特别是溅射1min金属钨的样品显示出优异的NO2室温探测能力与选择性,对4×10-6NO的气敏灵敏度是单纯多孔硅样品的 5.8倍。     

细胞计数分析传感器芯片的研究

细胞计数分析在医疗和科学研究方面有着广泛的应用,本论文叙述了一种有效结合了微流控分析和细胞传感器技术,并能够进行细胞计数分析的传感器芯片.通过微加工技术制备的聚二甲基硅氧烷(polydimethylsiloxane,PDMS)微通道,并将PDMS芯片键合在光寻址电位传感器(light-addressable potentiometric sensor,LAPS)的芯片表面,使其在硅基底上形成十字形、100 μm宽、30μm深的微流控通道,通过LAPS光生电流的传感测量,对重力驱动的大鼠血细胞进行了计数分析.该研究将微流控技术引入细胞传感器的研究,有利于后续通过细胞芯片实现细胞计数分析仪器的微型化和多功能化.     

SnO2纳米颗粒多孔薄膜气敏传感器对CO气体的敏感性

在适当的条件下分解有机金属前驱体[Sn(N(CH3)2)2]2制备了尺寸分布集中的SnO2纳米颗粒.二氧化锡纳米颗粒胶体沉积到气体传感器的硅基础结构上形成纳米颗粒多孔薄膜传感层,并对传感层进行金属Pt元素原位掺杂.在专用实验台上测定了SnO2纳米颗粒传感器对CO 气体的敏感性,结果显示此传感器对CO气体表现出较好的敏感性和稳定性.     

MEMS光声气体传感器光声腔的研究

光声气体传感器在微量气体探测方面具有许多优势,光声气体传感器的MEMS化可降低成本,提高传感器性能,具有重要的实际意义.依据光声传感器的原理,结合MEMS加工技术,研究制作了一种光声腔,同时,进行了一定的理论模拟,为实现光声气体传感器的MEMS化打下重要基础.     

In vitro assessing the risk of drug-induced cardiotoxicity by embryonic stem cell-based biosensor

Drug-induced prolongation of ventricular repolarization with arrhythmia is a major concern in clinic safety pharmacology, and has been a common reason for the withdrawal of several promising drugs from the market. Therefore, novel techniques should be developed to evaluate cardiotoxicity of new drugs in preclinical research. A cardiomyocyte based biosensor was developed using the light addressable potentiometric sensor (LAPS). Mouse embryonic stem cells cultured on the surface of LAPS were induced to differentiate into synchronized spontaneity beating cardiomyocytes. Changes of extracellular potentials and cell shapes with their mechanical beatings could induce modulation of photocurrents in the LAPS system, and finally change the output of the sensor. With the characteristics of light addressability, LAPS can record cell clusters at any desired position. The sensor can be used to record the prolongation of ventricular action potentials with the cardiotoxicity induced by drugs such as amiodarone, levofloxacin, sparfloxacin, and noradrenaline. The quick and on time characteristics of the sensor were promising to establish a high-throughput platform for pharmacological toxicity investigation.     

Constant-phase-mode operation of the light-addressable potentiometric sensor

The constant-phase-mode operation of the light-addressable potentiometric sensor (LAPS) is proposed and demonstrated. In this new operation mode, the temporal change and the spatial distribution of the analyte concentration are recorded in the form of the bias voltage applied to the LAPS sensor plate, which is servo-controlled to maintain the phase of the photocurrent at a constant value with respect to the light modulation. The constant-phase-mode LAPS is advantageous for its wider measurement range and reduction of artifacts.     

Phase-mode LAPS and its application to chemical imaging

The light-addressable potentiometric sensor (LAPS) is a chemical sensor based on the field effect in the electrolyte-insulator-semiconductor (EIS) structure. In the conventional LAPS, the ion concentration in the analyte solution in contact with the sensing surface is determined by measuring the amplitude of the photocurrent induced by illumination. In this study, we developed the phase-mode LAPS, in which the phase of the alternating photocurrent is measured instead of its amplitude. The phase-voltage curve shifted along the voltage axis in response to the variation of the ion concentration in analogy to the shift of the amplitude-voltage curve in the conventional LAPS. The primary advantage of the phase-mode LAPS is that it is much less sensitive to the variation of the light intensity. It is also demonstrated that chemical imaging based on the phase-mode LAPS is free from artifacts related to defects in the semiconductor substrate.     

光寻址电位式传感器在有机磷检测上的应用

通过将乙酰胆碱酯酶用戊二醛交联法固定在光寻址电位式传感器的H+敏Si3N4膜上,即可用于检测有机磷农药。介绍了该传感器的工作原理及制作方法,并且分析探讨了缓冲溶液和乙酰胆碱底液的影响,还给出了在不同抑制时间和不同有机磷浓度下的响应特性。实验表明这是一种简捷灵敏、高效实用的有机磷农药检测新方法。     

A high-density multi-point LAPS set-up using a VCSEL array and FPGA control

A new LAPS (light-addressable potentiometric sensor) set-up will be introduced, in which the light sources are miniaturised by the utilisation of a VCSEL (vertical-cavity surface-emitting laser) array to increase the measurement spot density. An FPGA (field-programmable gate array) is used to generate modulation signals for individual illumination of each measurement spot of the LAPS. The new set-up can operate a large number of measurement spots simultaneously by reading out the sum photocurrent and separate the signals of the individual measurement spots by an FFT analysis. The frequency, amplitude and offset of the modulation signal can be configured for each measurement spot by software. The new system can be combined with a positioning stage allowing the parallel read out of a single line of measurement spots and a scan perpendicular to that line in a similar manner, like for an optical scanner set-up. First measurements demonstrate the functionality of the new LAPS set-up as a chemical imaging system.     

非分光红外车辆尾气传感器系统设计

非分光红外( NDIR)是一种利用气体对红外光的选择性吸收原理进行气体浓度检测的方法.利用NDIR分析方法,设计一种车辆尾气检测传感器系统,实现对大气中存在的汽车尾气进行检测与分析.该系统使用多窗口红外传感器组合,采用v支持向量回归进行传感器建模.实验结果证明:该检测系统具有测量精度高、稳定性好和便于携带的特点,能够满足车辆尾气测量分析的需求.     

基于光电复合传感器的重金属检测算法的研究

基于光寻址电位传感器(Light Addressable Potentiometric Sensor,LAPS)和微电极阵列传感器(Microelectrode Array Sensor,MEA)的复合传感器件,作为无线传感器网络的敏感元件,已应用于水环境痕量重金属的检测中.本文基于该光电复合传感器,分别对LAPS及M...     

基于光电复合传感器的重金属原位监测系统嵌入式软件设计

针对水环境重金属监测仪器,设计了一种智能化嵌入式软件。该仪器采用光电复合传感器芯片,包括微阵列电化学传感器和光电位寻址传感器。仪器设计了可靠稳定的通信机制与上位机进行人机交互,仪器具有智能化自检功能,能够及时反馈传感器的故障信息。在检测过程中具有自动量程功能,能够最大发挥硬件优势,同时具有误差补偿和电路异常的检测机制。仪器具有高稳定性、自动化检测的特点,并完成了在野外无人值守的条件下的检测任务。     

基于VRML的远程教育虚拟实验室的研究

该文分析了VRML与同类技术相比的一些特点，并详细讨论了用VRML实现—虚拟实验室的关键方法。指出了简单的交互可用传感器节点，通过事件和路由来实现，复杂的交互可用Script节点，通过引入JAVA程序来实现。