Angle-resolved photoelectron spectroscopy of C60

The V3 loop of the gp120 of human immunodeficiency virus type 1 (HIV-1) is assumed to be involved in HIV-1-mediated membrane fusion. V3-derived peptides have been shown either to enhance or to prevent HIV-1 infection. Multibranched peptide constructs (MBPCs) derived from the V3 North American/European consensus sequence were designed to sort out these conflicting findings. At 5 microM, MBPC1 (8-branched GPGRAF) totally, and MBPC2 ([RKSIHIGPGRAFYT]4) partially, inhibited HIV-1LAI infection, whereas the GPGRAF monomer had only a limited effect. A peptide of the entire V3 consensus loop and a control MBPC had no detectable activity. The 5 microM MBPC1 HIV-1-inhibiting concentration was not cytotoxic, nor did it alter T lymphocyte allogeneic, antigen-, or mitogen-induced reactivities, and it was about 5- to 50-fold lower (MBPC2 and MBPC1, respectively) than that resulting in 50% cell death. Analysis of MBPC immunoreactivity showed that MBPC2, but not MBPC1, strongly reacted with human HIV-1 positive sera. Only MBPC2 elicited significant antibody responses in rabbits. The V3-derived MBPCs bound to CD4+ cells, as determined by immunofluorescence analysis. The binding was inhibited either by soluble CD4 or by CD4 monoclonal antibody (mAb) MT151, which recognizes the CDR3 region of the D1 domain of CD4, but not by other CD4 mAbs Leu3a, OKT4A, Q4021, 13B8-2, 5A8, RFT4, nor by the CD26 mAb BA5. Therefore, it appears likely that MBPCs inhibit HIV-1 infection by interacting with the CDR3 region of CD4 or with a region in its vicinity.     

Thermochemical data of alloys from photoelectron spectroscopy

It is shown, that the partial solution energy of an atom in an alloy contributes to the core level binding energy of this atom in the alloy. One can therefore use in turn core level binding energy shifts in alloys to determine the formation energy of a binary alloy. It is demonstrated that for the alloy systems PdAg, NiCu, AuCu, PdNi and PdCu there is good agreement between the thermochemical data measured directly and those extracted from the photoelectron spectra. In addition for an alloy system A_xB_1−x the partial solution energies of the atoms A + 1 and B + 1 in the dilute ternary systems A + 1 in A,_xb_1−x and B + 1 in A, _xb_l−x are obtained from the core level binding energy shifts. The approach is then used to predict the formation energies for the alloy systems CuZr, PdZr, and PdTi from their core level binding energy shifts as measured by photoelectron spectroscopy.