云南不同地点短葶飞蓬中野黄芩苷含量比较

[目的]比较云南不同产地短葶飞蓬中野黄芩苷的含量,为短葶飞蓬的良种选育及GAP栽培提供依据.[方法]用HPLC法测定短葶飞蓬中野黄岑苷的含量,色谱条件为:岛津Shim-pack VP-ODS液相色谱柱(150.0 mm×4.6 mm,5μg);流动相甲醇-0.1%磷酸水溶液(40:60,v/v);流速1.0 ml/min;检测波长335 nm;柱温25℃;进样量10μl.[结果]8个产地的短葶飞蓬中野黄芩苷的含量差异较大,昭通巧家药山短葶飞蓬野黄芩苷含量最高(2.628%),昆明双龙镇短葶飞蓬野黄苓苷含量最低(1.597%).[结论]该方法精密度、重现性好,适于短亭飞蓬中野黄岑苷含量的测定,为云南短葶飞蓬的良种选育及GAP栽培提供了依据.     

不同产地短葶飞蓬中总黄酮含量的比较

[目的]研究了不同产地短葶飞蓬中总黄酮含量异同.[方法]以紫外分光光度法(UV)对云南省9个不同地点的野生短葶飞蓬含量进行测定,并对结果进行方差分析.[结果]短葶飞蓬中总黄酮单株最高含量达18.539%,最低含量为3.653%;腾冲芒棒乡1平均含量最高,禄劝县雪山乡最低.同一产地点不同植株间含量差异明显,最大变异系数为25.6%,最小变异系数为9.7%.不同产地短葶飞蓬间总黄酮含量方差结果显示,F=19.467,P=0.000 1<0.01,LSD最小显著差数多重比较表明,不同地点短葶飞蓬中总黄酮含量差异性显著.[结论]短葶飞蓬中总黄酮含量的高低是内在遗传和外在生长环境共同作用的结果,该研究结果可为云南短葶飞蓬良种选育提供依据.     

菜芙蓉醇提物金丝桃苷含量测定及对肿瘤细胞生长抑制作用

[目的]建立测定菜芙蓉(Abelmoschus manihot(L.)Medic)醇提物金丝桃苷含量的方法以及研究菜芙蓉醇提物对Hela肿瘤细胞生长的抑制效果.[方法]采用体积分数为80%的乙醇提取总黄酮,HPLC法测定菜芙蓉不同植株部位中金丝桃苷含量.色谱柱为伊利特C<,18>(4.6 mm×250 mm,5 μm),流动相为甲醇-0.05%磷酸溶液(V/VWV,60:40),流速为1.0 ml/min,柱温为25℃,检测波长为360 nm和进样量为10μl.另外,MIT法测定菜芙蓉提取物体外抗Hela肿瘤细胞活性.[结果]金丝桃苷在0.04～0.4 mg/ml范围内与峰面积呈良好的线性关系,相关系数为0.999 8.幕芙蓉不同部位金丝桃苷含量分布为:花>叶>茎>根>籽,花中平均含量最高达4.002%.平均回收率和RSD值分别为99.23%和1.08%(n=6).菜芙蓉黄酮提取物能够有效地抑制Hela细胞的生长,IC<,50>约为228 μg/ml.[结论]该方法准确、简便、重复性好,可作为菜芙蓉金丝桃苷定量分析方法;菜芙蓉总黄酮具有抑制肿瘤细胞生长的作用.     

紫外可见分光光度法测定不同年份黄精中多糖含量

[目的]测定九华山地区所产不同育成年份黄精中多糖含量,为九华山地区黄精的产品分级和种植研究提供参考.[方法]采用苯酚-硫酸法对九华山地区产不同年份黄精中多糖含量进行测定.[结果]不同年份的黄精中多糖含量变化较大,随着种植年限的增加,多糖含量呈现升高趋势.其中,1年生黄精中多糖含量最少,为13.02%;9年生多糖含量达到最大,为18.44%;10年生多糖舍量出现下降,为15.64%.[结论]可以在实际采收中,通过不同育成年份对黄精进行分级.     

不同原产地苦荞种子中可溶性糖含量的比较

[目的]研究不同原产地苦荞种子中可溶性糖的含量差异.[方法]以不同原产地的35份苦荞样品为试验材料,采用比色法测定了其籽粒的可溶性糖含量.[结果]不同原产地的35份苦荞样品中的可溶性糖含量变化范围为7.23%～9.96%,平均值为8.17%;其中以贵州威宁产苦荞种子1374中可溶性糖含量最高,达9.96%;贵州威宁产种子苦荞T330中可溶性糖含量最低,为7.23%.[结论]该研究为苦荞植物资源的种植栽培和质量控制提供了科学依据.     

荧光分光光度法测定木香花中的总黄酮含量

[目的]测定木香花中的总黄酮含量.[方法]通过正交试验选择最佳提取条件,以芦丁为标样,用荧光分光光度法测定木香花中总黄酮的含量.[结果]在激发波长和发射波长为430和497 nm下,芦丁浓度与荧光强度呈良好的线性关系,标准曲线的相关系数为0.997 8,平均回收率为98.7%.RSD为0.19%.木香花中总黄酮的含量为1.09%.[结论]该方法简便、快速、准确,可用于植物及其制品中总黄酮含量的定量测定.     

渝产酸橙果实生长及有效成分积累动态的研究

[目的]研究渝产酸橙果实生长及有效成分积累动态规律,为确定川枳实、川枳壳适宜采收时期提供依据.[方法]通过定株取酸橙不同时期果实样品,测定其中辛弗林和柚皮苷含量.[结果] 随着酸橙果实的生长,其重量、纵横径不断增加.前期折干率高,6月中旬以后,折干率降低.酸橙辛弗林含量随着果实生长而降低,7月以后降到0.3%以下,辛弗林产量以6月中旬时最大;酸橙柚皮苷含量先增大后减小,在6月中下旬时达到最大,7月下旬之后显著下降,柚皮苷产量7月中旬时达到最大值.[结论]渝产酸橙采收枳实以6月中旬前为宜,采收枳壳应在7月中旬前后最好.     

浙贝母花的氨基酸成分分析

[目的]测定浙贝母花中氨基酸的种类和含量,为浙贝母花的资源开发提供科学依据.[方法]以采摘后自然阴干,冷冻干燥的和流化床干燥的浙贝母花作为研究对象.采用茚三酮柱后衍生-氨基酸分析法,测定3种处理下的浙贝母花的氨基酸含量.[结果]共测出17种氨基酸.并且氨基酸的总量在3种处理的浙贝母花中均高达200g/kg,其中谷氨酸含量最高,7种人体必需氨基酸均占所测氨基酸总量30.0%以上.[结论]浙贝母花及其相关产物具有较高的药用价值和保健价值,值得进一步开发.     

苦荞的蔗糖含量变异研究

[目的]明确不同苦荞资源间蔗糖含量的差异,筛选出低蔗糖含量的苦荞材料.[方法]测定了不同产地的35份苦荞籽粒中的蔗糖含量.[结果]35份苦荞资源的蔗糖含量变化幅度为0.0077%～0.2089%,平均值为0.0518%;不同产地苦荞的蔗糖含量存在差异,以贵州赫章地区的较高,贵州纳雍地区的较低.[结论]苦荞中蔗糖含量较低,可作为低糖粮食作物进行推广利用.     

大黄药材中大黄苷元含量测定

[目的]采用HPLC法测定不同产地的大黄药材中5种蒽醌苷元的含量.[方法]HPLC色谱条件为:色谱柱为Diamonsil C<,18>柱(5μm,250.0 mm x 4.6 mm);流动相为甲醇-0.05%磷酸水溶液(V:V=85:15);流速为1 mL/min;检测波长245 nm;进样量为20 μl.[结果]大黄各苷元线性关系良好,平均回收率为97.55%～100.87%,RSD值为1.38%～2.47%.大黄药材中各苷元含量范围分别为:芦荟大黄素0.26%～0.52%,大黄酸0.21%～1.47%,大黄素0.26%～0.40%,大黄酚0.29%～1.95%,大黄素甲醚0.48%～2.72%.[结论]该方法精密度良好、重现性、稳定性好,适合大黄药材中各大黄苷元的质量控制.     

Regional differences in suicide in Hungary in the light of registered cases of depression

Author made a revision on the theory published by Rihmer and alii in the year 1989. According to this theory the marked regional differences of suicide in Hungary are caused by the different discovery of the "endogen depression" (rate of diagnosed depression). So the lower is the diagnosis of depression the higher is the suicide rate in a given territory. They have seen a negative correlation between the number of working physicians and the suicide rates. Author made a reexamination on a correct database between 1985-1994. He has also made a study using the DSM-III-R diagnostic criteria in four counties of Hungary. His results deny the theory above mentioned. The rank-correlation of the number of GP and the suicide rates in Hungary in years 1985-1994 did not show correlation. So the underdiagnosis of the depressive disorders and the different number of GP are not the cause of the regional differences of suicide in Hungary.     

Role of clozapine in the occurrence of chromosomal abnormalities in human bone-marrow cells in vivo and in cultured lymphocytes in vitro

Bone-marrow chromosomes were examined from 38 mentally and physically retarded and two psychiatric patients who were being treated with a variety of neuropharmacologic drugs. Twenty of these patients used clozapine (Leponex). The clastogenic effects of clozapine in vitro were studied in the lymphocyte cultures of three patients--one free of hematologic disease and two who 6 months earlier had had agranulocytosis attributed to the use of clozapine. The mean frequency of cytogenetic abnormalities in the bone-marrow cells of patients who used clozapine was significantly increased (P less than 0.05). The two patients who had had agranulocytosis had a greater frequency of cytogenetic abnormalities in their cultured lymphocytes in vivo and in vitro than the patient free of hematologic disease. A clone with a 13/14 chromosome translocation was detected in one of the patients. As all patients received a number of drugs during the in vivo and in vitro studies no definite conclusions could be drawn regarding the role played by clozapine in the occurrence of chromosomal abnormalities.     

Inhibitory effect of suramin in rat models of angiogenesis in vitro and in vivo

The aim of the present study was to test the ability of the chemotherapeutic agent suramin to inhibit angiogenesis in experimental models in vitro and in vivo. In the culture of rat aortic rings on fibronectin, suramin dose-dependently inhibited vascular cell growth, achieving the maximal effect (mean - 88% versus controls, P < 0.05) at 400 microg/ml. Image analysis showed that suramin could inhibit microvessel sprouting in fibrin from rat aortic rings as evaluated by the ratio between the cellular area and the mean gray value of the sample (sprouting index); suramin at 50 microg/ml significantly reduced the sprouting index from the control value of 0.35+/-0.04 to 0.14+/-0.02 mm2/gray level (P < 0.05). Likewise, the area occupied by cells was 19.2+/-1.8 mm2 as compared with 41.8+/-4.2 mm2 in controls (P < 0.05). In the rat model of neovascularization induced in the cornea by chemical injury, suramin at 1.6 mg/eye per day reduced the length of blood vessels (0.7+/-0.1 mm as compared with 1.5+/-0.1 mm in controls, P < 0.05). In the same model the ratio between the area of blood vessels and the total area of the cornea (area fraction score) was decreased by suramin from 0.19+/-0.02 in controls to 0.03+/-0.003 (P < 0.05). Suramin given i.p. at 30 mg/ kg per day markedly inhibited the neovascularization induced in the rat mesentery by compound 48/80 or conditioned medium from cells secreting the angiogenic protein fibroblast growth factor-3 (FGF-3). The area fraction score in control rats treated with compound 48/80 was 0.31+/-0.03, and this was reduced to 0.07+/-0.01 by suramin (P < 0.05). After i.p. administration of FGF-3 the area fraction score was reduced by suramin from 0.29+/-0.03 to 0.05+/-0.01 (P < 0.05). These results provide evidence that suramin exerts inhibitory effects on angiogenesis in both in vitro and in vivo models.     

Difference in changes of plasma volume in two types of Goldblatt hypertension in rabbits

High plasma renin concentration (PRC) and hematocrit (Ht) were observed in hypertensive rabbits with one renal artery clipped and an intact opposite kidney (Group A), while rather low PRC and Ht in hypertensive ones with one renal artery clipped and the opposite kidney removed (Group B). Plasma volume (PV), blood volume (BV), and packed cell volume (PCV) were estimated in these two types of hypertensive rabbits, and also in sham-operated rabbits (Group C). In Group A, 4 weeks after clipping of a renal artery, PV was decreased and significant inverse correlations were observed between PV and the elevation of blood pressure and between PV and PRC. A significant correlation between PRC and the elevation of blood pressure was also found 4 and 12 weeks after the clipping. PCV was increased until 12 weeks after clipping. In Group B, PV was increased 1 and 4 weeks after clipping, then returned to the initial level. Ht was decreased 1 and 12 weeks, and PCV was decreased 12 weeks after clipping. These results would indicate that a high Ht seen in Group A was partly due to the decreased PV and partly due to the increased PCV, while, in Group B, a low Ht in early stage might be mainly due to the increased PV, but in the late stage, a decreased PCV appeared to play a role to lower Ht. PRC seems to be affected differently by PV in these two types of hypertension.     

CS1000在天铁烧结生产过程控制中的应用

结合天津铁厂烧结分厂二烧车间烧结过程控制的具体情况,介绍了分散型控制系统在烧结生产上的应用以及数据网上发布的一种方法。     

Epidemiology of trichinellosis in lynx in Finland

To examine the possibility of cytokine gene therapy in relation to pancreatic cancer, we evaluated the antitumor effect of human pancreatic carcinoma cells (AsPC-1) which were retrovirally-transduced with several kinds of cytokine genes. These cells were inoculated into BALB/c nude mice and their tumor volumes were assessed. The in vitro growth rate of the transduced cells was not different from that of a parental cell line. Among the transduced cells, human interleukin (IL)-6-transduced AsPC-1 and mouse granulocyte macrophage colony-stimulating factor-transduced AsPC-1 cells showed a significant retardation of tumor growth compared with a parental cell line. In the cases of AsPC-1 cells transduced with the human IL-2 or mouse IL-4 gene, small tumors were generated but thereafter they regressed completely. Histological examinations showed monocytic cell infiltration around the tumors of IL-2- or IL-4-producing cells. These data suggest that secretion of IL-2 or IL-4 from tumor cells can induce an antitumor effect even in the defective condition of mature T cells.     

Efficacy of lomefloxacin in treatment of nonspecific inflammatory diseases and in prophylaxis of postoperative complications in urology

Clinical and bacteriological efficacies of lomefloxacin and ofloxacin were comparatively estimated in the treatment of 104 urologic patients with nonspecific inflammatory diseases and in 36 urologic patients for prophylaxis of inflammatory complications after surgical interventions. The majority of the urinary tract pathogens i.e. Enterobacteriaceae spp. and gramnegative nonfermenting bacteria were highly susceptible to both the fluoroquinolones. The clinical and bacteriological efficacies of lomefloxacin amounted to 94.4 and 80.7 per cent and those of ofloxacin to 92 and 79.2 per cent respectively. The prophylactic use of lomefloxacin and ofloxacin was efficient in 94.7 and 94.1 per cent of the cases respectively. The antibacterial prophylaxis in urologic patients should be applied not only at the period of surgical interventions but also during the early postoperative period.