Associations between serotonin transporter gene promoter region (5-HTTLPR) polymorphism and gaze bias for emotional information.

The serotonin transporter promoter region polymorphism (5-HTTLPR) is associated with neural response to negative images in brain regions involved in the experience of emotion. However, the behavioral implications of this sensitivity have been studied far less extensively. The current study used eye-tracking methodology to examine how individuals genotyped for the 5-HTTLPR, including the single nucleotide polymorphism (SNP) rs25531, allocated attention during prolonged (30-s) exposure to face stimuli depicting positive and negative emotion. Short 5-HTTLPR allele carriers and carriers of the long allele with guanine at the sixth nucleotide (S/LG) displayed a stronger gaze bias (total fixation time, number of fixations, mean fixation length) for positive than for sad, threat, or neutral stimuli. In contrast, those homozygous for the long 5-HTTLPR allele with adenine at the sixth nucleotide (LA) viewed the emotion stimuli in an unbiased fashion. Time course analyses indicated no initial 5-HTTLPR group differences; however, S/LG 5-HTTLPR allele carriers were more likely than LA 5-HTTLPR homozygotes to direct gaze toward happy than toward sad stimuli over time. This bias toward positive stimuli during the later stages of information processing likely reflects a strategic effort to downregulate heightened reactivity to negative stimuli among 5-HTTLPR S/LG allele carriers. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Association of the serotonin transporter gene promoter region (5-HTTLPR) polymorphism with biased attention for emotional stimuli.

A deletion polymorphism in the serotonin transporter-linked polymorphic region (5-HTTLPR) has been associated with vulnerability to affective disorders, yet the mechanism by which this gene confers vulnerability remains unclear. Two studies examined associations between the 5-HTTLPR polymorphism and attentional bias for emotional stimuli among nondepressed adults. Biased attention, attention engagement, and difficulty with attention disengagement were assessed with a spatial cuing task using emotional stimuli. Results from Study 1 (N = 38) indicated that short 5-HTTLPR allele carriers experienced greater difficulty disengaging their attention from sad and happy stimuli compared with long allele homozygotes. Study 2 participants (N = 144) were genotyped for the 5-HTTLPR polymorphism, including single nucleotide polymorphism rs25531 in the long allele of the 5-HTTLPR. Consistent with Study 1, individuals homozygous for the low-expressing 5-HTTLPR alleles (i.e., S and LG) experienced greater difficulty disengaging attention from sad, happy, and fear stimuli than high-expressing 5-HTTLPR homozygotes. Because this association exists in healthy adults, it may represent a susceptibility factor for affective disorders that becomes problematic during stressful life experiences. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The serotonin transporter promoter polymorphism and childhood positive and negative emotionality.

Association studies of the serotonin transporter promoter polymorphism (5-HTTLPR) and negative emotionality (NE) are inconclusive. However, emerging evidence suggests that the association between this polymorphism and NE may be influenced by levels of another temperament trait, positive emotionality (PE). Therefore, this study examined whether the association between the 5-HTTLPR and NE was moderated by PE. A community sample of 413 three-year-old children completed a standardized battery of laboratory tasks designed to tap temperamental emotionality. Children were also genotyped for the 5-HTTLPR. No direct association between 5-HTTLPR genotype and NE was found. However, the interaction of child PE and NE predicted 5-HTTLPR genotype. Furthermore, children with a short allele who were also low in PE had significantly greater NE than children without a short allele or children with high PE. Our findings suggest that the short allele of the 5-HTTLPR is associated with NE only in the context of low PE. Inconsistent links between NE and this gene in previous research may stem from the failure to consider other temperament traits that moderate associations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Interaction effect of D4 dopamine receptor gene and serotonin transporter promoter polymorphism on the cortisol stress response.

Genetic variation of the serotonin transporter (SCL6A4, 5-HTT) has been associated with fear- and anxiety-related behaviors, while a polymorphism in exon III of the D4 dopamine receptor gene (DRD4) has been linked to novelty seeking. The dopaminergic and the serotonergic neurotransmitter system have been found to modulate the amygdala-connected circuitries that are crucial in emotional modulation and response to fearful stimuli. Additionally, reactivity of amygdala-innervated effector systems is also essential for our understanding of anxiety-related behaviors. Here, we used the stress-induced activation of the hypothalamic-pituitary-adrenal axis to investigate the impact of 5-HTTLPR and DRD4 on the cortisol stress response in 84 healthy adults. Saliva cortisol was measured during and after the Trier Social Stress Test. We found a significant main effect of DRD4: Carriers of the 7R allele exhibited lower cortisol responses. Additionally, a DRD4 by 5-HTTLPR interaction emerged: 5-HTTLPR LA/LA homozygotes showed a lower cortisol response than did S or LG allele carriers but only if they possessed at least one copy of the DRD4 7R allele. The results point to independent and joint effects of these polymorphisms on stress responsivity. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Variation in the serotonin transporter gene modulates selective attention to threat.

The 5-HTTLPR is an insertion/deletion polymorphism in the promoter region of the serotonin transporter gene. Prior research has revealed associations between the short-allele variant of this polymorphism, enhanced self-reported negative emotionality, and hypersensitivity of fear relevant neural circuits. In a sample of 50 healthy women we examined the role of 5-HTTLPR for cognitive-affective processing of phylogenetical fear-relevant stimuli (spiders) in a dot probe task. In contrast to homozygote long-allele carriers (ll), participants carrying at least 1 short allele (ss and sl) selectively shifted attention toward pictures of spiders, when these were presented for a duration of 2,000 ms. These results argue for an involvement of 5-HTTLPR in cognitive processing of threatening stimuli and thus, underpin its general role for individual differences in negative affect. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Volunteer client adult attachment, memory for in-session emotion, and mood awareness: An affect regulation perspective.

In this study, the authors examined relations between volunteer client adult attachment and both (a) memory for negative affect occurring within the first session of therapy and (b) mood awareness (mood labeling and mood monitoring). Participants were 80 volunteer clients (students with a personal issue who volunteered to participate in the research for credit) who participated in 2 counseling sessions with a counselor trainee. As hypothesized, higher levels of attachment anxiety were associated with (a) greater memory for negative affect and (b) more mood monitoring but, contrary to expectation, were not associated with mood labeling. Also contrary to expectation, attachment avoidance was not associated with a minimizing strategy with respect to memory for emotion or monitoring of mood, but it was negatively related to mood labeling. As hypothesized, some evidence emerged for an Attachment Anxiety × Avoidance interaction in predicting memory for negative affect. There were no significant Attachment Anxiety × Avoidance interactions in predicting mood labeling or mood monitoring. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

No association between the serotonin transporter-linked promoter region polymorphism and either schizophrenia or density of the serotonin transporter in human hippocampus

BACKGROUND: Allelic association case-control analysis of a deletion/insertion polymorphism in the serotonin transporter-linked polymorphic region (5-HTTLPR) has suggested associations with unipolar disorder, bipolar disorder, depression, and Alzheimer's disease. Moreover, the heterozygous long form of the 5-HTTLPR has been associated with increased levels of mRNA for the serotonin transporter (5-HTT) and increased serotonin uptake in lymphoblastic cell lines. This study attempts to determine whether there is an association between 5-HTTLPR genotype and schizophrenia or the binding of [3H]paroxetine to the human hippocampal 5-HTT. MATERIALS AND METHODS: DNA from the cerebellum from 58 schizophrenic and 62 control subjects was used to genotype the 5-HTTLPR. In addition, the relationship between 5-HTTLPR genotype and the affinity and density of [3H]paroxetine binding to the hippocampal 5-HTT was assessed. RESULTS: There were no associations between 5-HTTLPR allotype or genotype and/or the parameters of [3H]paroxetine binding to the hippocampal 5-HTT. CONCLUSIONS: Our data suggest that 5-HTTLPR genotype neither confers an increased susceptibility for schizophrenia nor dictates the expression of the 5-HTT in the human hippocampus.     

Attachment at (not to) work: Applying attachment theory to explain individual behavior in organizations.

In this article, we report the results of 2 studies that were conducted to investigate whether adult attachment theory explains employee behavior at work. In the first study, we examined the structure of a measure of adult attachment and its relations with measures of trait affectivity and the Big Five. In the second study, we examined the relations between dimensions of attachment and emotion regulation behaviors, turnover intentions, and supervisory reports of counterproductive work behavior and organizational citizenship behavior. Results showed that anxiety and avoidance represent 2 higher order dimensions of attachment that predicted these criteria (except for counterproductive work behavior) after controlling for individual difference variables and organizational commitment. The implications of these results for the study of attachment at work are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Maternal representations of past and current attachment relationships, and emotional experience across the transition to motherhood: A longitudinal study.

Sad and anxious feelings are known to increase in the immediate postpartum period, whereas studies on new mothers' other emotional qualities such as anger are scarce. In laboratory studies, attachment security was found to be associated with effective emotion regulation in challenging situations. This study investigated attachment representations of experiences with parents and of current experiences with the partner as predictors of sad, anxious, and angry feelings across the transition to motherhood. Seventy-seven pregnant women in their third trimester were administered the Adult Attachment Interview and the Current Relationship Interview. The Differential Emotions Scale was given in pregnancy and at the infant's ages of 2 weeks, 2, 4, and 6 months, asking both mothers and fathers about maternal emotional experience. Sadness and anxiety increased 2 weeks postpartum and returned to below baseline over the following months, while anger did not change. Contrary to mothers with an insecure representation of their couple relationship, those with a secure representation reported and displayed increased sadness and anxiety 2 weeks after giving birth, from which they quickly recovered. For mothers secure in their representation of past attachment relationships with parents, an increase of low-level anger emerged 4 months postpartum, which did not occur in insecure participants and receded quickly. It can be concluded that secure representations of current and past attachment relationships help new mothers express and recover from negative emotions. These findings further elucidate the associations between attachment status and emotion regulation while adding a couple perspective. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Preventive resources and emotion regulation expectancies as mediators between attachment and college students' stress outcomes.

In this study the authors examined both preventive psychological coping resources and negative mood regulation expectancies as potential mediators between parental attachment and two types of stress outcomes: stress symptoms and stress-produced emotions. Data were collected from 390 college students and separate structural equation models were tested for the outcomes of stress symptoms and emotions. Results suggested that for both models, as hypothesized, preventive resources and negative mood regulation expectancies functioned as mediators. Further, there was evidence that these results were similar for the model in which stress symptoms were used as an outcome, as well as the model in which stress-produced emotions were used as the outcome. Implications for a more complete understanding of psychological resources promoted by secure attachment are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Working models of attachment to parents and partners: Implications for emotional behavior between partners.

This study examined whether working models of attachment are associated with observed positive emotion, sadness, and anger during marital conflict. Individuals (n = 176) from a longitudinal study of families participated in the current cross-sectional study. Narrative interviews assessed the unique and combined contribution of attachment representations based on parents (adult attachment) and partner (couple attachment). The influence of partner’s attachment, depression symptoms, and sex of participant was also examined. Hierarchical linear models demonstrated that one’s couple attachment security predicts one’s observed positive emotion, whereas the partner’s couple attachment security predicts one’s observed negative emotion. Partner’s depression symptoms moderated the effects of partner’s couple attachment. Adult attachment was not related to observed emotional behavior between partners. These findings have important clinical implications for individual, couple, and family therapy. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Application of adult attachment theory to group member transference and the group therapy process.

Although clinical researchers have applied attachment theory to client conceptualization and treatment in individual therapy, few researchers have applied this theory to group therapy. The purpose of this article is to begin to apply theory and research on adult dyadic and group attachment styles to our understanding of group dynamics and processes in adult therapy groups. In particular, we set forth theoretical propositions on how group members’ attachment styles affect relationships within the group. Specifically, this article offers some predictions on how identifying group member dyadic and group attachment styles could help leaders predict member transference within the therapy group. Implications of group member attachment for the selection and composition of a group and the different group stages are discussed. Recommendations for group clinicians and researchers are offered. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Differences between Taiwanese and U.S. cultural beliefs about ideal adult attachment.

Some researchers believe that important tenets of attachment theory are culturally universal, whereas others claim that key constructs are rooted in Western values and should not be generalized further. To explore possible cultural differences in adults, undergraduates from Taiwan (n = 280) and the United States (n = 268) were asked in the present study to complete a self-report measure of adult attachment, the Experiences in Close Relationships Scale (K. A. Brennan, C. L. Clark, & P. R. Shaver, 1998), as they believed "an ideally emotionally and psychologically healthy person of your own gender in your culture" would respond. Findings suggested significant differences by cultural group, gender, and Gender x Culture interactions. Taiwanese women and men endorsed more avoidance in beliefs about ideal adult attachment than their U.S. counterparts, and Taiwanese men endorsed more anxiety than U.S. men. These cultural differences were not explained by group differences in independent and interdependent self-construal. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The influence of adult attachment styles on the association between marital adjustment and depressive symptoms.

This study tested the hypothesis that attachment styles moderate the relationship between marital adjustment and depressive symptoms among husbands and wives. In a sample of 91 married couples, ratings of the anxious-ambivalent attachment style moderated the relationship between marital adjustment and depressive symptoms for both husbands and wives. Additionally, ratings of the secure attachment style moderated the relationship between marital adjustment and depressive symptoms for wives, with a trend for husbands. These findings suggest a relationship between insecurity and a predisposition to depressive symptoms in marital relationships. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Examining the relationship between current attachment status and freely recalled autobiographical memories of childhood.

This study investigated the relationship between individual differences in attachment and the free recall of childhood memories. Specifically, it focuses on how attachment anxiety and attachment avoidance, using the Experiences in Close Relationship Scale self-report, relate to the affective quality and the presence of caregivers in memories from childhood. Participants were 79 undergraduate and masters students attending a northeast university. Participants completed a memory task designed to elicit freely recalled memories from childhood. They then rated the affective valence (positive/negative) and intensity of each memory, and identified memories in which caretakers were present. Attachment avoidance was related to recalling more negative memories involving caretakers and was negatively related to the average intensity of memories involving caretakers. The results support and extend previous research suggesting that affect regulation strategies employed by individuals high in attachment anxiety and high in attachment avoidance are linked to differences in how information about the past is recalled. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Serotonin transporter genetic variation and biased attention for emotional word stimuli among psychiatric inpatients.

The short allele in a variable repeat sequence of the promoter region of the serotonin transporter gene (5-HTTLPR) has been associated with stronger activation in brain regions critical for processing emotional stimuli. The authors examined whether variants of the 5-HTTLPR promoter polymorphism were also associated with individual differences in attentional biases for emotional stimuli. Words related to anxious and dysphoric emotional states were presented to psychiatric inpatients in a standard dot-probe reaction time task. Compared with participants with two long alleles, carriers of the short 5-HTTLPR allele exhibited a stronger attentional bias for anxious word stimuli. No genetic group difference was observed for dysphoric word stimuli. Findings from this preliminary study highlight the potential for integrating genetic and cognitive models of psychopathology. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The relationship between adult attachment style and therapeutic alliance in individual psychotherapy: A meta-analytic review.

The present study examined the relationship between adult attachment style and therapeutic alliance in individual psychotherapy. Search procedures yielded 17 independent samples (total N = 886, average n = 52, standard deviation = 24) for inclusion in the meta-analysis. Results indicated that greater attachment security was associated with stronger therapeutic alliances, whereas greater attachment insecurity was associated with weaker therapeutic alliances, with an overall weighted effect size of r = .17, p .10) with the exception of the source of alliance ratings; results indicated that patient-rated alliance demonstrated a significantly larger relationship with attachment compared with therapist-rated alliance (Qbetween = 3.95, df = 1, p = .047). Implications for clinical practice and future research are discussed. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Associations between adult attachment ratings and health conditions: Evidence from the National Comorbidity Survey Replication.

Objective: Attachment insecurity has been hypothesized to be a risk factor for the development of disease and chronic illness. This study was the first to investigate associations between adult attachment ratings and a wide range of health conditions. Design: Cross-sectional data from the National Comorbidity Survey Replication (N = 5645) were used. Measures: Participants completed Hazan and Shaver's (1987) measure of adult attachment and provided reports regarding 15 health conditions. Results: Logistic regression analyses that adjusted for demographic variables indicated that avoidant attachment ratings were positively associated with conditions defined primarily by pain (e.g., frequent or severe headaches). Anxious attachment ratings were positively associated with a wider range of health conditions, including several involving the cardiovascular system (i.e., stroke, heart attack, high blood pressure). Secure attachment ratings were unrelated to the health conditions. Additional analyses investigated whether the attachment ratings accounted for unique variance in the health conditions beyond that accounted for by lifetime histories of depressive, anxiety, and alcohol- or substance-related disorders. In these analyses, anxious attachment ratings continued to have significant positive associations with chronic pain, stroke, heart attack, high blood pressure, and ulcers. Conclusion: The findings were generally supportive of the theory that insecure attachment is a risk factor for the development of disease and chronic illness, particularly conditions involving the cardiovascular system. Further research regarding the role of attachment in the development of specific health conditions is warranted. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Discriminative stimulus properties of (±)-fenfluramine: The role of 5-HT? receptor subtypes.

The role of serotonin 5-HT? receptors (5-HT?R) in the discriminative stimulus effects of fenfluramine was investigated. Male Sprague-Dawley rats were trained to discriminate (±)-fenfluramine (2 mg/kg ip) from saline using a 2-lever, water-reinforced paradigm. Drug-lever responding after fenfluramine was dose-dependent. The 5-HT2C/1BR agonist mCPP and the 5-HT2CR agonist MK 212 fully substituted, whereas the 5-HT2A/2CR agonist DOI partially substituted, for the training drug. The 5-HT2BR agonist BW 723C86 engendered saline-lever responding. The 5-HT2C/2BR antagonist SB 206553 completely antagonized the fenfluramine discrimination as well as the full substitutions of mCPP and MK 212 and the partial substitution of DOI. The selective 5-HT2AR antagonist M100907 partially suppressed the stimulus effects of fenfluramine, mCPP, and MK 212 and almost fully attenuated the partial substitution of DOI. RS 102221, a selective 5-HT2CR antagonist that does not cross the blood-brain barrier, did not alter the fenfluramine cue. Results demonstrate that the discriminative stimulus effects of fenfluramine are centrally mediated by 5-HT2CR and to some extent by 5-HT2AR. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

CCR5 promoter polymorphism and HIV-1 disease progression. Multicenter AIDS Cohort Study (MACS)

BACKGROUND: The rate of progression to AIDS varies among individuals infected with HIV-1. Factors responsible include two inherited human alleles, CCR5 delta32 and CCR2-641, which alter the protein-coding regions for the HIV-1 coreceptors/chemokine receptors CCR5 and CCR2b. We tested the hypothesis that polymorphisms of the CCR5 promoter might affect the rate of progression of HIV-1 infected people to AIDS. METHODS: We used directed heteroduplex analysis to identify polymorphism in the CCR5 promoter. Promoter-variants were compared in vitro with a chloramphenicol acetyltransferase reporter gene, and in vivo by genotyping HIV-1 seroconvertors discordant at polymorphous loci. FINDINGS: An A/G polymorphism was identified at basepair 59029 (Genbank U95626) in the CCR5 promoter. Both promoter alleles were common (43-68% allelic frequency for 59029-A depending on race). When in-vitro promoter activity was measured, 59029-G had 45% lower activity than 59029-A (p=0.05). In a cohort of HIV-1 seroconvertors lacking both CCR5 delta32 and CCR2-641, 59029-G/G individuals progressed to AIDS on average 3.8 years more slowly than 59029-A/A individuals (p=0.004). 59029-G/A discordance did not correlate with discordant rates of infection. INTERPRETATION: Our results are consistent with the hypothesis that CCR5 is important in HIV-1 pathogenesis. CCR5 59029-G/G appears to be protective relative to CCR5 59029-A/A, and about twice as protective relative to CCR5 delta32 or CCR2-641. This effect may be the result of reduced CCR5 mRNA production. These results identify the first site in the CCR5 promoter that may be a useful target for treatment of HIV-1 infection.