利用紫外线三维荧光光谱分析方法提取潜在指纹

通过光谱分析实验,分别测定石英和白板载片上的富含皮脂潜在指纹的二维和三维荧光光谱,并利用波长260～300nm的紫外线激发指纹残留物,从而获得波长310～380nm范围的指纹荧光光谱.实验数据验证了运用紫外荧光照相方法提取潜在指纹的结果.     

分色片在紫外照相中的应用

利用CS型分色片作紫外照相中的感光材料，较好地改善了弱反差痕迹的 紫外照相效果，为检验鉴定提供了方便。     

鸡心、鸡血中抗氧化物的提取及其活性研究

从鸡心、鸡血中提取抗氧化物 ,并分别利用邻苯三酚 (PR)自氧化法、碘量法对其活性进行了检测。结果表明 :鸡心提取物比鸡血提取物的抗氧化活性强。     

指纹荧光显现试剂双水杨醛缩乙二胺合锌的合成

以水杨醛、乙二胺为原料,在碱性条件下,经优化反应条件,合成了双水杨醛缩乙二胺,并在此基础上选取合适的碱金属元素锌作为中心离子反应,优化反应条件,合成新型Schiff base型配位化合物荧光显现剂。利用元素分析确定目标产物化学式,并利用红外光谱对产物结构进行了表征。合成指纹荧光显现剂在特定波长激发下具有理想的荧光效果,有利于后期的开发和实践运用,并在其他领域也具有广泛应用。     

氧化铝干柱固相萃取结合紫外导数光谱法快速测定全血安定

建立一种简便的血中安定的测定方法.采用氧化铝干柱层析固相萃取血中安定,紫外二阶导数光谱法测定萃取液中安定药物浓度.对于血液中安定含量在mg/L级时,pH=7条件下的萃取回收率〉89%,RSD〈10%.本方法简便易行、材料价廉,对于含安定mg/L级的血样,测定结果准确可靠,批量测试更省时间.     

光稳定剂复配对核壳WPC抗紫外老化性能的影响

为探索经济、环保且效果更好的木塑复合材料(WPC)表面耐老化处理方法,采用共挤技术,以不同复配方式将纳米白炭黑、HALS–L944及UV327添加于核壳型WPC壳层。对试样进行紫外光加速老化试验,并对其弯曲性能、表面颜色及化学成分结构进行了测试表征与方差分析。研究结果表明,当壳层加入1%纳米白炭黑与1%UV327复配时效果最好,紫外老化2 500 h后,较之空白样,WPC的弯曲强度保留率提高了14.7%,弹性模量保留率提高了17.4%,保护效果最好,具有一定的协同作用;表面颜色变化较为缓慢,色差下降了44.9%,优于单独添加光稳定剂效果;O/C值增长幅度为9.1%,氧化的碳/未氧化的碳比值增加了11.8%,表面氧化程度最低。说明纳米白炭黑与UV327具有一定的协同作用,能够更好地提高核壳WPC的抗紫外老化性能,降低其光氧化降解程度。     

酶法提取猪血中血红素的工艺研究

研究了酶法提取猪血中血红素的工艺条件,确定了用20万U·g-1中性蛋白酶从猪血中提取血红素的最佳工艺条件为:加酶量2%、底物浓度5%、酶解温度50℃、酶解时间10 h,在此条件下,血红素的相对得率达到77.2%.     

鸡血中乙酰胆碱酯酶的提取研究

选取新鲜鸡血为乙酰胆碱酯酶的酶源,对其进行了高速离心、透析、盐析和凝胶柱层析等分离纯化。在pH=7.5缓冲液中,30℃下保温30 min等最佳测试条件下,得到的酶活力为U=0.112(μmol/min),比活力2.24。     

SPE-GC-MS方法检验全血中多虑平

目的:建立人全血中多虑平的固相萃取-气相质谱联用仪检测方法。方法全血中的多虑平以SKF525A为内标,应用waters HLB固相萃取小柱提取净化,应用气相质谱联用仪SIM方法进行定性定量检验。结果多虑平检出限为1.0 ng/mL,多虑平质量浓度在0.01~10μg/mL范围内线性良好(r≥0.998),平均萃取回收率为62.8%~71.6%,日内、日间精密度RSD≤6.42%(n=5)。结论该方法专属性强,去干扰能力好,灵敏及准确。     

超临界CO_2流体萃取枇杷叶中熊果苷的工艺研究

采用超临界CO2流体萃取枇杷叶中的熊果苷,并用紫外可见分光光度法测定萃取物中熊果苷含量;在单因素实验的基础上,通过L9（34）正交试验对萃取条件进行优化,确定最佳工艺参数为：萃取温度55℃,萃取压力20 MPa,携带剂用量75 mL,萃取时间30 min。在此工艺条件下萃取得到的产物杂质含量少,产品纯度高,结果重现性良好;工艺参数在现有工业条件下容易实现,具有可行性。     

Fluorescence Recognition of Anions Using a Heteroditopic Receptor: Homogenous and Two-Phase Sensing

In contrast to monotopic receptor 3, the anthracene functionalized squaramide dual-host receptor 1 is capable of selectively extracting sulfate salts, as was evidenced unambiguously by DOSY, mass spectrometry, fluorescent and ion chromatography measurements. The receptors were investigated in terms of anion and ion pair binding using the UV–vis and ¹H NMR titrations method in acetonitrile. The reference anion receptor 3, lacking a crown ether unit, was found to lose the enhancement in anion binding induced by the presence of cations. Besides the ability to bind anions in an enhanced manner exhibited by ion pair receptors 2 and 4, changing the 1-aminoanthracene substituent resulted in their exhibiting a lower anion affinity than receptor 1. By using receptor 1 and adjusting the water content in organic phase it was possible to selectively detect sulfates both by “turn-off” and “turn-on” fluorescence, and to do so homogenously and under interfacial conditions. Such properties of receptor 1 have allowed the development of a new type of sensor capable of recognizing and extracting potassium sulfate from the aqueous medium across a phase boundary, resulting in an appropriate fluorescent response in the organic solution.     

Color rendering: A tale of two metrics

It was the best measure of color rendering, it was the worst measure of color rendering. Color rendering index (CRI) is the most common metric used by the lighting industry to represent the color rendering properties of electric light sources. CRI was intended to characterize how “true” or “natural” objects appeared when illuminated by a light source, but was never intended to, for example, represent how well object colors could be differentiated under a light source, another important aspect of color rendering. Data presented here demonstrate that CRI in conjunction with another measure of color rendering, gamut area index (GAI), is useful at predicting subjective judgments of how “natural” objects appear as well as how “vivid” objects appear, and how well one can discriminate between subtle differences in hue. Neither measure by itself, however, is sufficient for meeting all of the expectations of a light source for providing good color rendering under all viewing conditions. It remains for future research to determine if just two metrics are sufficient to assure good color rendering from a light source and whether these two metrics (CRI and GAI) are the best for such purpose. In the meantime, CRI and GAI should be used jointly in recommendations as practical, useful, and mutually reinforcing measures of color rendering. The data presented here also demonstrate that total irradiance is important for good color rendering. © 2008 Wiley Periodicals, Inc. Col Res Appl, 33, 192–202, 2008