Preparation of a hydrophobic cerium oxide nanoparticle coating with polymer binder via a facile solution route

In this work, cerium oxide (CeO₂) nanoparticles (NPs) were synthesized using a facile, low temperature solution process and coated using spin coating and spray coating approaches, for the fabrication of a hydrophobic surface coating. Silicon wafer (Si) substrates coated with CeO₂ NPs exhibited excellent hydrophobic behavior, but poor adhesion of the NPs to the substrate was observed - likely due to the low surface polarity of CeO₂ NPs. Polyacrylic acid (PAA) was introduced as an adhesion promoter to improve NP surface characteristics and obtain an adherent and cohesive coating. Slight polarity tuning and binder inclusion significantly enhanced the binding capability of the NPs as determined by peel-off measurements. The superior mechanical properties of NP coatings were attributed to the incorporation of PAA in the polymeric network. It improves inter-particle and particle-substrate secondary interactions, ultimately aiding NP cohesion and adhesion when deposited onto the Si substrate. The adhesive and hydrophobic properties of CeO₂ NP coatings were maintained upon exposure to high temperatures, and the coatings are transparent as well, making them suitable for various applications, such as cookware, glass coating and technology components.     

Influence of embedded ZnAlCe-NO3− layered double hydroxides on the anticorrosion properties of sol–gel coatings for aluminum alloy

A protective coating was designed by dispersing Ce-doped ZnAl layered double hydroxides (ZnAlCe-LDHs) nanoparticles in hybrid sol–gel (SiO_x/ZrO_x) layer on aluminum alloy AA2024. The concentration of cerium in synthesized LDHs was varied to ascertain the optimum condition for anticorrosion performance. The LDH nanoparticles were characterized in terms of structure, morphology and chemical composition. It was found that Ce (III) was inserted into the sheets of LDHs and two mixture phases of LDHs and CeO₂ were formed when the atomic ratio of Ce/(Al + Ce) was higher than 0.05. The sol–gel coating embedded with LDHs (Ce/(Al + Ce) = 0.1) exhibited high corrosion resistance, probably due to the synergistic inhibition of ZnAlCe-LDHs and CeO₂ nanoparticles.     

Characterization of surface-modified ceria oxide nanoparticles synthesized continuously in supercritical methanol

Surface-modified ceria oxide (CeO₂) nanoparticles were synthesized continuously in supercritical methanol at 400 °C, 30 MPa and a residence time of ∼40 s using a flow type reactor system. Oleic acid and decanoic acid were used as the surface modifiers. Transmission electron microscopy (TEM) showed that the surface modifiers changed drastically the shape and size of the nanoparticles. When 0.3 M of the surface modifiers were used, primary particles with diameter of 2–3 nm loosely aggregated and formed secondary particles with size of 30–50 nm. Wide angle X-ray diffraction (WAXD) analysis revealed that the surface-modified nanoparticles retained CeO₂ crystalline structure. The surface-modified CeO₂ nanoparticles had a very high surface area (140–193 m²/g) compared to the unmodified CeO₂ particles synthesized in supercritical water (8.5 m²/g). Fourier transform infrared (FT-IR) and thermogravimetric analysis (TGA) indicated that aliphatic, carboxylate and hydroxyl groups were chemically bounded on the surface of CeO₂ nanoparticles. Dispersability test using ultraviolet transmittance showed that most of the surface-modified CeO₂ nanoparticles were dispersed in ethylene glycol for 30 days while the unmodified CeO₂ particles synthesized in supercritical water or in supercritical methanol were precipitated after 7–15 days.     

Plant-based synthesis of cerium oxide nanoparticles as a drug delivery system in improving the anticancer effects of free temozolomide in glioblastoma (U87) cells

Nowadays, nanocarriers were proven to contain the potential of improving cancer treatments and are utilized to carry anticancer medications to tumors. In this study, cerium oxide nanoparticles (CeO₂-NPs) were synthesized by using Caccinia macranthera leaf extract as the stabilizer and reducer agent, as well as cerium nitrate salt as the supplier source of cerium. The synthesized CeO₂-NPs were analyzed through different procedures such as UV–Vis, FTIR, FESEM/EDX/PSA, XRD, XPS, and TGA/DTA. The outcomes of XRD and FTIR analyses con?rmed the synthesis of pure and crystalline structures of CeO₂-NPs. The average size and zeta potential of our nanoparticles were about 30 nm and ?18.5 mV, respectively. According to the results of XPS analysis, the percentage of Ce⁴⁺ was more than that of the Ce³⁺ oxidation state in synthesized NPs. The CeO₂-NPs were loaded with Temozolomide (TMZ) as an anti-cancer drug through electrostatic interaction and the produced nano-drug (CeO₂-TMZ) was delivered to glioblastoma multiforme (GBM) tumor cells. In conformity to the observations, the drug loading content (DLC) and drug loading efficiency (DLE) of CeO₂-TMZ were about 89.10 and 20.29, respectively. In comparison to the TMZ drug, the in vitro assay exhibited the exertion of higher antiproliferative activities, cell cycle arrest, apoptosis, and expression of p53 by CeO₂-TMZ, which proves the promising capability of this drug as a remedial factor for cancer treatment.     

Construction of titania–ceria nanostructured composites with tailored heterojunction for photocurrent enhancement

Titania–ceria (TiO₂–CeO₂) nanostructured composites based on the design of coating the surfaces of anodized TiO₂ nanotube arrays with small band gap CeO₂ nanoparticles have been constructed and characterized to demonstrate the effectiveness of the TiO₂–CeO₂ semiconductor heterojunction in enhancing the photocurrent response of TiO₂-based photoelectrodes. The TiO₂–CeO₂ heterojunction was confirmed to possess conduction and valence band offsets (0.81 and 1.59 eV, respectively) which promote the separation of photoinduced electron–hole pairs. The photocurrent densities of the TiO₂–CeO₂ composites prepared with low annealing temperatures were about 25–40% larger than that of the anatase TiO₂ nanotube arrays. When the nanoparticle-on-nanotube architecture of the TiO₂–CeO₂ heterostructure was maintained under specific processing conditions, the benefits of having a high specific surface area, a small band gap component capable of absorbing visible light, and a favorable heterojunction were achieved together for photocurrent enhancement.     

Supercapacitive properties,optical band gap,and photoluminescence of CeO2–ZnO nanocomposites prepared by eco-friendly green and citrate sol-gel methods: A comparative study

CeO₂–ZnO nanocomposite was synthesized using a cost-effective and environmentally friendly green method utilizing a natural extract of Hibiscus Sabdariffa L. The structural parameters, morphology, optical, and supercapacitive properties of a novel green sol-gel synthesized CeO₂–ZnO nanocomposite (CZO-g) were compared with those of a citrate sol-gel auto-combustion derived nanocomposite (CZO-s). XRD confirmed the formation of cubic and wurtzite hexagonal binary phases, and CZO-g exhibited better crystallinity than CZO-s. ZnO incorporation inhibits the accumulation of CeO₂ semispherical NPs, as demonstrated by FESEM images. DRS and PL emission measurements confirmed that the optical band gap of nanocomposites was widened. CV, GCD and EIS measurements were used to determine the electrochemical supercapacitive behavior of nanomaterials deposited on Ni Foams in a 3 M KOH electrolyte. At a current density of 1 A g^?1, CZO-g exhibited significantly greater specific capacitance (431 F g^?1) than CZO-s and CeO₂-g. Furthermore, CZO-g retained 89.4% of its initial capacitance after 3000 cycles at a high current density of 7 A g^?1, indicating that it could be a promising candidate for a high-performance supercapacitor.     

Evaluation of physicochemical and biological properties of SnO2 and Fe doped SnO2 nanoparticles

In recent decades, nanoparticle synthesis has been used for various physical and chemical methods. However, different toxic chemicals are used during this synthesis process to address these concerns, which has multiple effects on environmental toxicity and high cost. To avoid these problems, we need a cost-effective and environmentally friendly approach. In this study, green synthesis was used to make tin oxide (SnO₂) and ferrous doped tin oxide (SFO) nanoparticles (NPs) from Morinda citrifolia leaf extracts. The X-ray diffraction patterns of SnO₂ and SFO NPs reveal a tetragonal crystalline structure. From the FESEM image of synthesized SnO₂ and SFO NPs, their spherical structure and chemical composition were identified by EDX spectrum. Through the DLS spectrum, the hydrodynamic size was observed at 66 and 61 nm for SnO₂ and SFO NPs, respectively. In the FTIR spectrum, the O–Sn–O stretching vibration peak arises at (606 & 509 cm^?1 for SnO₂ NPs) and (613 & 538 cm^?1 for SFO NPs). Photoluminescence is used in materials to detect surface defects and impurity levels. The antibacterial activity of the SnO₂, SFO NPs, and conventional antibiotics like amoxicillin NPs is effectively inhibited against S. aureus and E. coli bacterial strains. SFO NPs exhibit a higher antibacterial activity as compared to SnO₂ and amoxicillin. The anticancer efficacy of increased SFO NPs compared to SnO₂ NPs was tested against (MDA-MB-237) human breast cancer cells. These results suggest that Fe ions modified SnO2 NPs could be used in healthcare industrial applications to improve human health.     

Microwave-assisted synthesis of ZnO doped CeO2 nanoparticles as potential scaffold for highly sensitive nitroaniline chemical sensor

Herein, we report the successful fabrication of highly sensitive, reproducible and reliable nitroaniline chemical sensor based on ZnO doped CeO₂ nanoparticles. The ZnO doped CeO₂ nanoparticles were synthesized through a simple, facile and rapid microwave-assisted method and characterized by several techniques. The detailed characterizations confirmed that the synthesized nanoparticles were monodisperse and grown in high density and possessing good crystallinity. Further, the synthesized ZnO doped CeO₂ nanoparticles were used as efficient electron mediators for the fabrication of high sensitive nitroaniline chemical sensors. The fabricated nitroaniline chemical sensor exhibited very high sensitivity of 550.42 μA mM⁻¹ cm⁻² and experimental detection limit of 0.25 mM. To the best of our knowledge, this is the first report in which CeO₂–ZnO nanoparticles were used as efficient electron mediators for the fabrication of nitroaniline chemical sensors. Thus, presented work demonstrates that ZnO doped CeO₂ nanoparticles are potential material to fabricate highly efficient and reliable chemical sensors.     

Green synthesis of TiO2 nanospheres from isolated Aspergillus eucalypticola SLF1 and its multifunctionality in nanobioremediation of C. I. Reactive Blue 194 with antimicrobial and antioxidant activity

For synthesis of titanium dioxide (TiO₂) nanoparticles (NPs), Green methods have been proven to be more efficient than several physiochemical methods. This article presents a non-toxic, ecofriendly, cost-effective and a facile route of green synthesis of TiO₂ NPs by an isolated fungus Aspergillus eucalypticola SLF1, which exhibits excellent photocatalytic, antimicrobial and antioxidant activity without structural modification done by physicochemical methods. The TiO₂ NPs are characterized by UV–Visible spectroscopy, XRD, FTIR, FE-SEM, DLS, TEM, BET, Raman spectroscopy and PL. The mesoporous, anatase phase, with a band gap 3.49 eV observed by BET, XRD. UV–Visible spectral analysis displayed sunlight driven photocatalytic performance against C. I. Reactive Blue 194 by advanced oxidation process. Decolourization and 99.70% degradation within 30 min exhibited pseudo first order kinetic with reaction rate constant 0.1935 min⁻¹ by linear method. These findings are superior physicochemical methods. Ecofriendly degradation was confirmed by UV–Vis. HPLC and LCMS etc and phytotoxic studies.     

Fabrication of corrosion-resistant Al2O3–CeO2 composite coating on AA7075 via plasma electrolytic oxidation coupled with electrophoretic deposition

Al₂O₃–CeO₂ composite coating was fabricated on AA7075 by combining plasma electrolytic oxidation (PEO) with electrophoretic deposition (EPD). CeO₂ nanoparticles are electrophoretically incorporated into the plasma electrolytic oxidized Al₂O₃ coatings by the synergetic effect of PEO and EPD processes. The passivation behavior of Al₂O₃ and inhibiting nature of CeO₂ have been studied by the electrochemical corrosion analysis in 3.5 wt% NaCl solution and salt spray corrosion test (SSCT) in 5 wt% NaCl as per ASTM standards. The results showed that the Al₂O₃–CeO₂ composite coating via PEO coupled EPD significantly improved the corrosion resistance (~10³ times higher R_p) compared to the plasma electrolytic oxidized Al₂O₃ coating.     

pH Responsive Abelmoschus esculentus Mucilage and Administration of Methotrexate: In-Vitro Antitumor and In-Vivo Toxicity Evaluation

The rapid progression in biomaterial nanotechnology apprehends the potential of non-toxic and potent polysaccharide delivery modules to overcome oral chemotherapeutic challenges. The present study is aimed to design, fabricate and characterize polysaccharide nanoparticles for methotrexate (MTX) delivery. The nanoparticles (NPs) were prepared by Abelmoschus esculentus mucilage (AEM) and chitosan (CS) by the modified coacervation method, followed by ultra-sonification. The NPs showed much better pharmaceutical properties with a spherical shape and smooth surface of 213.4–254.2 nm with PDI ranging between 0.279–0.485 size with entrapment efficiency varying from 42.08 ± 1.2 to 72.23 ± 2.0. The results revealed NPs to possess positive zeta potential and a low polydispersity index (PDI). The in-vitro drug release showed a sustained release of the drug up to 32 h with pH-dependence. Blank AEM -CS NPs showed no in-vivo toxicity for a time duration of 14 days, accompanied by high cytotoxic effects of optimized MTX loaded NPs against MCF-7 and MD-MBA231 cells by MTT assay. In conclusion, the findings advocated the therapeutic potential of AEM/CS NPs as an efficacious tool, offering a new perspective for pH-responsive routing of anticancer drugs with tumor cells as a target.     

Characterization of charging effect of citrate-capped Au nanoparticle pentacene device

Capacitance–voltage hysteresis for a non-volatile memory was realized in a metal–pentacene–insulator–silicon (MPIS) device using gold (Au) nanoparticles (NPs) intervened between pentacene and SiO₂ insulator. A memory window higher than 2.0 V was obtained under (±) 5 V programming sweeping range. The SiO₂ as thick as 30 nm was adopted as the dielectric layer, and 3-aminopropyl-triethoxysilane (APTES) was used for self-assembling of Au NPs monolayer. In addition, citrate-functionalized Au NPs was dip-coated and used as charge storage elements. Formation of a monolayer of the Au NPs was confirmed by HR-SEM and AFM. Capacitance–voltage hysteresis in this study was resulted from the charge storage in the layer of Au NPs.     

Silver-zinc oxide nanocomposite: From synthesis to antimicrobial and anticancer properties

A green method by Verbascum speciosum was used to synthesize zinc oxide nanoparticles (ZnO NPs). ZnO NPs were coated with silver to synthesize Ag–ZnO nanocomposite (NCs). The physicochemical properties of Ag–ZnO NCs were analyzed by Fourier-transform infrared spectroscopy (FTIR), powder X-ray diffraction (PXRD), field emission scanning electron microscope (FESEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential. The FTIR indicated the peak of Zn–O vibration and some hydroxyl and carboxyl groups. PXRD analyses confirmed the synthesis of ZnO NPs and Ag–ZnO NCs. Due to the size of the crystallite obtained from PXRD, solid-phase sizes (from FESEM and TEM images), and dynamic sizes from DLS, agglomeration was observed. The Ag–ZnO NCs showed a negative charge surface (?49.3 mV). Ag–ZnO NCs had a high antibacterial activity towards two most important infectious bacteria (i.e., Escherichia coli and Staphylococcus aureus) and anticancer activity against human liver-carcinoma cells (HepG2). Later, it depended on time and concentration of Ag–ZnO NCs. The cytotoxicity properties of Ag–ZnO NCs were also studied against NIH-3T3 as a normal cell, where the results verified the lower cell toxicities of nanocomposite than the HepG2.     

Preparation of ZnFe2O4–chitosan-doxorubicin hydrochloride nanoparticles and investigation of their hyperthermic heat-generating characteristics

In this study, the structural morphology and magnetic effects of magnetic ZnFe₂O₄ nanoparticles loaded with the cancer-fighting drug doxorubicin hydrochloride (DOX-HCl) were investigated. These nanoparticles have been found to have potential biomedical applications in targeted drug-delivery systems. The zinc ferrite nanoparticles were prepared by a chemical coprecipitation method and coated with chitosan. The nanoparticles were loaded with DOX-HCl and their surfaces improved by folic acid, which can be activated to target specific cancer cells. The specific absorption rate (SAR) values of the ZnFe₂O₄–chitosan–DOX-HCl nanoparticles were investigated at a frequency of 200 kHz and 1.5 kA/m amplitude in order to obtain Brownian relaxation time parameters. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), and ultraviolet–visible spectrophotometry (UV–vis) were used to characterize the bulk properties of these nanoparticles. In addition, the impact of the nanoparticles under an alternating current (AC) magnetic field and their heat-generation ability were investigated using an experimental setup. The average nanoparticle size was found to be 8.5 nm. Magnetic hysteresis loops confirmed the superparamagnetism of the nanoparticles. The saturation magnetization was 6 emu/g. UV–vis was used to measure the amount of drug loaded onto the nanoparticles. The amount of drug absorption was significantly higher after 12 h, totaling 75%. The specific absorption rate parameter was 80.66 W/g, and the Brownian relaxation time was 188×10⁻⁹ s.     

Hydrophobic to hydrophilic surface transformation of nano-scale zinc ferrite via oleic acid coating: Magnetic hyperthermia study towards biomedical applications

Nanoscale spinel zinc ferrite (ZNF) was prepared by sol-gel auto-ignition route and subsequently its surface was modified by oleic-acid (OA) coating. The pristine and surface modified ZNF nanoparticles (UC-ZNF and OA-ZNF) were characterized by standard techniques. XRD patterns of both the samples ensured the nanocrystalline mono-phasic cubic-spinel lattice structure with ‘Fd-3m space-group’. FT-IR spectra revealed the presence of vibrational frequency-modes of spinel structure and successful coating of OA over ZNF. The nano-size spherical grains with some agglomeration and OA coating over ZNF were visualized in FE-SEM images. The hydrophobic-to-hydrophilic surface-transition of ZNF was confirmed by water contact-angle measurements. The BET surface-area and distribution of pore-radius was evaluated by recording N₂-isotherms. The M − H plots confirmed the superparamagnetic nature of both the samples. Optical properties were studied by UV–Vis and PL spectroscopy techniques. The colloidal-stability and distribution of particle-sizes were estimated by zeta-potential and DLS measurements. Magnetic hyperthermia studies were carried out for different concentrations (2, 4, 6, 8 and 10 mg/mL) of both the samples. The biocompatible nature of both the samples was studied by cell-viability studies. All these results ensure the implementation of OA-ZNF nanoparticles with minimum dose rate (8 mg/mL) in magnetic hyperthermia therapies for cancer treatment.     

Phytoextract-mediated synthesis of Cu doped NiO nanoparticle using cullon tomentosum plant extract with efficient antibacterial and anticancer property

In the present study, nickel oxide (NiO) and copper-doped nickel oxide (NiCuO) nanoparticles (NPs) were successfully synthesized using Cullen tomentosum plant extract with the co-precipitation method. This work focuses on the Phyto-mediated synthesis and characterization of NPs for their biological applications. Phytochemicals that exist in the plant extract acts as reducing and capping agent. The successful formation of the NPs was validated by various analysis as XRD, FESEM, EDAX, FT-IR, UV–Vis, and Photoluminescence. According to XRD studies, the average crystallite size of NiO and NiCuO NPs is 36 nm and 31 nm, respectively. The river stone and nanoflower like morphology for NiO and NiCuO NPs are confirmed by FESEM image. Furthermore, the synthesized NPs were tested against Gram-positive (Bacillus subtilis, Streptococcus pneumoniae) and Gram-negative (Klebsiella pneumoniae, Escherichia coli) bacteria, which showed enhanced antibacterial activity of NiCuO NPs. The cytotoxicity of NPs was investigated against human breast cancer cells (MDA-MB-231) and fibroblast L929 cell lines. Also, the IC₅₀ value for human breast cancer cells is 11.8 μg/mL. According to these findings, NiCuO NPs are potential nanomaterials with advanced healthcare uses.     

Protein corona: implications for nanoparticle interactions with pulmonary cells

Background

We previously showed that cerium oxide (CeO₂), barium sulfate (BaSO₄) and zinc oxide (ZnO) nanoparticles (NPs) exhibited different lung toxicity and pulmonary clearance in rats. We hypothesize that these NPs acquire coronas with different protein compositions that may influence their clearance from the lungs.

Methods

CeO₂, silica-coated CeO₂, BaSO₄, and ZnO NPs were incubated in rat lung lining fluid in vitro. Then, gel electrophoresis followed by quantitative mass spectrometry was used to characterize the adsorbed proteins stripped from these NPs. We also measured uptake of instilled NPs by alveolar macrophages (AMs) in rat lungs using electron microscopy. Finally, we tested whether coating of gold NPs with albumin would alter their lung clearance in rats.

Results

We found that the amounts of nine proteins in the coronas formed on the four NPs varied significantly. The amounts of albumin, transferrin and α-1 antitrypsin were greater in the coronas of BaSO₄ and ZnO than that of the two CeO₂ NPs. The uptake of BaSO₄ in AMs was less than CeO₂ and silica-coated CeO₂ NPs. No identifiable ZnO NPs were observed in AMs. Gold NPs coated with albumin or citrate instilled into the lungs of rats acquired the similar protein coronas and were cleared from the lungs to the same extent.

Conclusions

We show that different NPs variably adsorb proteins from the lung lining fluid. The amount of albumin in the NP corona varies as does NP uptake by AMs. However, albumin coating does not affect the translocation of gold NPs across the air-blood barrier. A more extensive database of corona composition of a diverse NP library will develop a platform to help predict the effects and biokinetics of inhaled NPs.

     

Photocatalytic reduction of Hg using core–shell Fe/CeO2 hollow sphere nanocomposites

A sol–gel method was used to prepare Fe/CeO₂ hollow sphere nanocomposites. For comparison, a direct calcination of cerium nitrate was used to prepare CeO₂ nanoparticles and Fe/CeO₂ nanoparticles. The photocatalytic reduction of Hg was used to study the photocatalytic performance of the prepared nanocomposite photocatalysts using visible-light irradiation. The BET surface areas of the CeO₂ nanoparticles and CeO₂ hollow spheres were 76 and 160 m²/g, respectively. The BET surface area of the hollow sphere CeO₂ and CeO₂ nanoparticles decreased to 145 and 57 m²/g, respectively, by adding iron nanometal. The TEM results revealed that the shapes of the CeO₂ nanoparticle and hollow sphere materials are spherical nanoparticles and uniform nanospheres, respectively. The Fe/CeO₂ nanoparticles and Fe/CeO₂ hollow spheres are spherical nanoparticles and core–shell, respectively. The photocatalytic performance by the Fe/CeO₂ hollow spheres was 50, 3.9, and 1.4 times more efficient than that observed from the CeO₂ nanoparticles, Fe/CeO₂ nanoparticles, and CeO₂ hollow spheres, respectively.     

Mechanistic investigation of aluminide coating formation by low-pressure chemical vapor deposition method on NiCoCrAlY coating in presence of nickel-ceria composite layer

In this study, a hybrid coating comprised of NiCoCrAlY fabricated by HVOF method, Ni–CeO₂ composite coated by electrodeposition, and aluminide coating applied by low pressure chemical vapor deposition (LPCVD) method are investigated. To elucidate the formation process of aluminide coating, the microstructure and properties of the applied coatings were examined by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and EDS analyses. It was concluded that the desired β-NiAl phases are uniformly created within a single step on the surface. Furthermore, with the extending of the coating duration from 2 to 4 h, the thickness of the aluminide coating was increased from 14 to 25 μm. The thickness values were increased even further in the presence of Ni–CeO₂ coating, where the growth mechanism was also changed. Within 4 h, a coating with a thickness of roughly 50 μm was obtained. Moreover, in the presence of Ni–CeO₂ coating, it was observed that the inward diffusion of aluminum was predominant at the beginning of the process, whereas with longer processing durations, the outward diffusion of the nickel becomes dominant instead.     

Studies on the effect of long-chain and head groups of nonionic surfactants synthesized from palm and coconut oil on the formation of silver nanoparticles

Four fatty amides were synthesized using coconut and palm oil sources. The chemical structure of the as-prepared fatty acids was confirmed using Fourier Transform Infrared (FT-IR) and ¹H, ¹³C NMR spectroscopy. The effect of different chain lengths and head groups of these compounds on Ag nanoparticles (NPs) synthesis was investigated. Ultraviolet–visible spectroscopic studies showed characteristic absorbance peaks (λ_max ≈ 410 nm). FT-IR results indicated that the surfactant functionalities are responsible for the Ag NPs stability. The effect of fatty amides on the morphology and size distribution of the Ag NPs was investigated using transmission electron microscope (TEM). The TEM micrographs showed the formation of fine spherical morphology due to surfactant-mediated self-assembly with an average particle size of 1–3 nm. Dynamic light scattering (DLS) analysis showed the micellar self-assembly of Ag NPs-fatty amides. The effect of surfactant on the solution behavior was analyzed using surface tension measurements. Cocamide and palm DEA showed relatively low free energy (∆G_mic) values, resulting in smaller particles with good distribution. Finally, the Ag NPs showed outstanding antimicrobial activity against Pseudomonas putida bacteria.