Effects of fiber addition on antioxidant capacity and nutritional quality of wheat bread

Wholegrain and high fiber foods are recognized as nutritious and healthful products due to their content of dietary fiber, antioxidants and bioactive compounds. In this study, wheat, rye, barley, oat wholegrain flours and two fibers namely cellulose (insoluble fiber) and xanthan gum (soluble fiber) were used to replace a portion of wheat flour in pan bread to study effects of fibers on phenolic compounds, antioxidant capacity, dietary fiber fractions, and starch digestibility in vitro. Incorporation of wholegrain flours increased free and bound phenolics and antioxidant capacity of all the breads examined to various extent depending on the source of fiber. Additionally, soluble, insoluble and total dietary fiber fractions and total minerals increased with the addition of wholegrain flours or fibers. Rapidly and slowly digestible and resistant starches were not significantly influenced by adding wholegrain flours or fibers to breads. The study demonstrates the importance of enriching wheat bread with wholegrain flours to boost antioxidants and dietary fibers. On the other hand, more research is required to better understand impact of wholegrain flours or fiber on starch digestibility in vitro and glycemic response.     

Total phenolic and total flavonoid content,antioxidant activity and sensory evaluation of pseudocereal breads

The aim of the study was to investigate the effect of adding (in two different doses 15% and 30%) pseudocereal (buckwheat, amaranth and quinoa) flour on the antioxidant properties and sensory value of breads. Buckwheat flour had the highest phenolic content (7.25 ± 0.23 mg/g dw). The content of total flavonoids in flours was about 2–4 fold higher when compared to breads. The addition of buckwheat flour to wheat bread, particularly in higher dose, was more effective in enhancing antioxidant activity, as evaluated by means of FRAP and DPPH, which increased by 2.36 fold, and 3.64 fold respectively, in comparison with other pseudocereal flours (amaranth, quinoa), which caused, in higher doses, the changes of above parameters within the ranges 1.20–1.79 fold, and 0.60–1.71 fold. Analysis of sensory results of breads showed that addition of buckwheat flour to the dough might improve subjective properties of bread and increase acceptable quality attributes such as taste, colour or odour. All these observations suggest that addition of buckwheat flour into bread can improve antioxidant as well as sensory properties of bread. Bread fortified with pseudocereal flours, and especially with buckwheat flour, may be placed on the market as a functional food.     

Baking properties and microstructure of pseudocereal flours in gluten-free bread formulations

In the present study, the baking properties of the pseudocereals amaranth, quinoa and buckwheat as potential healthy and high-quality ingredients in gluten-free breads were investigated. Scanning electron micrographs were taken of each of the flours. The pasting properties of these flours were assessed using a rapid visco analyser. Standard baking tests and texture profile analysis were performed on the gluten-free control and pseudocereal-containing gluten-free breads. Confocal laser scanning microscopy (CLSM) images were also obtained from the baked breads and digital image analysis was conducted on the bread slices. Bread volumes were found to significantly increase for the buckwheat and quinoa breads in comparison with the control. In addition, the pseudocereal-containing breads were characterised by a significantly softer crumb texture effect that was attributed to the presence of natural emulsifiers in the pseudocereal flours and confirmed by the confocal images. No significant differences were obtained in the acceptability of the pseudocereal-containing gluten-free breads in comparison with the control.     

Techno‐functional properties of wheat flour‐resistant starch mixtures applied to breadmaking

Resistant starch can be used to reduce the availability of carbohydrates in baked products. In this study, the effect of type 4 resistant wheat starch (RS₄) on wheat flour dough and breads was evaluated. Wheat flour was substituted by RS₄ at 10%, 20% and 30% w/w (RS10, RS20 and RS30, respectively). Rheological and thermal behaviours of dough were evaluated. Besides, bread quality, starch digestibility and bread staling were analysed. All substituted dough exhibited viscoelastic behaviour but lower elastic and viscous moduli. Regarding to bread quality, specific volume and crumb texture were negatively affected in samples with RS₄. However, all samples were technologically acceptable. During storage, crumb hardening was observed in breads without and with RS₄ but amylopectin retrogradation was not particularly affected. The in vitro digestibility of bread with RS showed a lower release of reducing sugars and a lower estimated glycaemic index, suggesting a healthier profile for these breads.     

Impact of sourdough fermented with Lactobacillus plantarum FST 1.7 on baking and sensory properties of gluten-free breads

Sourdoughs were produced from buckwheat, oat, quinoa, sorghum, teff and wheat flour using the heterofermentative lactic acid bacteria Lactobacillus plantarum FST 1.7 and added to a basic bread formulation of flour from the same grain type (20 % addition level). Dough rheology, textural (crumb hardness, specific volume) and structural bread characteristics (crumb porosity, cell volume, brightness) of sourdough-containing breads were compared to non-sourdough-containing breads (control). Changes in protein profiles as analysed with capillary electrophoresis were observed in all sourdoughs. Furthermore, sourdough addition led to decreased dough strength resulting in softer dough. No influences on specific volume and hardness on day of baking were found for gluten-free sourdough breads. The staling rate was reduced in buckwheat (from 8 ± 2 to 6 ± 2 N/day) and teff sourdough bread (13 ± 1 to 10 ± 4 N/day), however, not significantly in comparison with the control breads. On the contrary, in wheat sourdough bread, the staling rate was significantly reduced (2 ± 1 N/day) in comparison with control bread (5 ± 1 N/day). Sourdough addition increased the cell volume significantly in sorghum (+61 %), teff (+92 %) and wheat sourdough breads (+78 %). Therefore, crumb porosity was significantly increased in all gluten-free and wheat sourdough breads. Shelf life for sourdough breads was one (teff and oat), two (buckwheat, quinoa and sorghum) and 3 days (wheat) and was not prolonged by sourdough addition. The inferior aroma of breads prepared from the gluten-free flours was also not improved by sourdough addition.     

Influence of Gluten-free Flours and their Mixtures on Batter Properties and Bread Quality

Gluten is a major component of some cereals and is responsible for flour technological characteristics to make bakery products. However, gluten must be eliminated from the diet of celiac patients because its ingestion causes serious intestinal damage. The objectives of this study were to assess the effect of different flours and their mixtures on thermal and pasting properties of batters, and to study the quality parameters and staling rate of gluten-free breads. Starch gelatinization temperatures and enthalpies depended on batter composition. Soy flour addition had a higher effect on rice than on corn starch, indicating some differential interaction between starch and proteins. Inactive soy flour incorporation improved all bread quality parameters in both corn- and rice-based breads. Higher batter firmness of formulations with soy addition (extrusion force was doubled in rice/soy and rice/corn/soy batters with regard to rice and rice/corn batters) partially explained higher specific volume (rice breads: 1.98 cm³/g; rice/soy 90:10 2.51 cm³/g, corn/soy 90:10: 2.05 cm³/g, whereas corn/soy 80:20: 2.12 cm³/g), as these batters retained more air during proofing. The staling rate was decreased by soy flour incorporation on rice (staling rate of rice breads with 10% soy diminished 52%, and with 20% of soy addition, 77%, both regarding to 100% rice breads) and corn formulation (the staling rate of corn/soy 80:20 breads was 5.9% lower than corn/soy 90:10) because of the high water-holding capacity of soy proteins and the interactions established with amylopectin that could retard the retrogradation process. Breads made with rice, corn, and soy flours showed the best quality attributes: high volume, good crumb appearance, soft texture, and low staling rate.     

Effect of the shape of rice starch granules on flour characteristics and gluten‐free bread quality

The effect of three different rice varieties with different starch shapes (Seolgaeng (SG), round starch structure; Samkwang (SK), polygonal starch structure and Boramchan (BRC), polygonal starch structure) on rice flour characteristics and gluten‐free bread baking quality was investigated. Rice flours were produced by dry milling and passed through a 200 mesh sieve. Electron microscopy revealed that the structure of SG grains, with round starch granules, possessed larger void spaces than SK and BRC, composed of polygonal starch granules. For this reason, SG grain had low grain hardness and consequently, it was milled to a fine flour with low damaged starch content. The thermo‐mechanical properties were determined by Mixolab, which revealed that SG was gelatinised rapidly and maintained high viscosity after gelatinisation. These characteristics gave SG flour the ability to build up bread structure without gluten. Specific volume and crumb hardness of gluten‐free rice breads made of SG, SK and BRC flours were 3.37, 3.11 and 2.12 mL g^?1 and 2.61, 2.76 and 6.46 N, respectively. The SG flour with round starch structure is appropriate for making gluten‐free rice breads.     

Effects of wheat and rye bread structure on mastication process and bolus properties

Chemical composition, baking process and structure of breads influence their degradation in digestion leading to different postprandial responses. Rye bread has a very different structure as compared to wheat bread, and rye breads are known to induce lower postprandial insulin responses than wheat bread. The aim of this study was to find out potential differences in mastication and initial starch hydrolysis rate of rye and wheat breads. Three rye breads (wholemeal rye, endosperm rye and endosperm rye with gluten) and wheat bread were masticated by fifteen participants and the process was monitored using electromyography. The particle size distribution and initial in vitro starch hydrolysis of the bread boluses were analysed. Specific volume correlated negatively and closed porosity of breads correlated positively with work required for mastication. When compared to wheat bread, wholemeal rye bread required more work for mastication process (p = 0.004). Rye breads were degraded to smaller particles than wheat bread during mastication. There was a trend (p = 0.098) towards slower in vitro starch hydrolysis rate in rye bread boluses than in wheat bread boluses. The results indicate that the digestion process of rye breads differs from that of wheat bread already in the early phase of digestion. This may be one reason behind the unique postprandial responses reported for rye breads.     

Impact of Heat Moisture Treatment and Hydration Level of Flours on the Functional and Nutritional Value-Added Wheat-Barley Blended Breads

The impact of heat moisture treatment (HMT) of flours on the techno-functional and nutritional patterns of binary flour bread matrices (wheat/barley, WT/CB, 60:40, w/w) was investigated in untreated (?) and HMT (+) samples made at 160 and 170 dough yield (DY) levels. Assessment was performed by determining viscoelastic (stress relaxation test) and mechanical (double compression test) behaviours, volume (seed displacement), colour (Photoshop system), crumb grain (digital image analysis), starch digestibility (enzyme hydrolysis) and staling kinetics (Avrami equation), bioaccessible polyphenol content (digestive enzymatic mild extraction) and anti-radical activity (DPPH●). A superior functional profile was provided by HMT of CB flour in the blend WT?CB+ when hydrated at DY 170 compared to the untreated control WT?CB?. The sample exhibited a similar specific volume, more cohesive, springier, more resilient crumb, with similar rate and extent of crumb firming on ageing, and similar colour pattern but finer and more uniformly sized cell structure, and deserved similar sensory ratings as the control WT?CB? concerning cell uniformity, smoothness and typical smell and taste. Digestible starch kinetic curves of blended breads pointed out samples WT?CB+ and WT+CB+ as matrices expliciting a lower degree and slower rate of starch hydrolysis when mixed at low and high DY, respectively. A similar anti-radical activity for composite bread matrices was evidenced regardless of either HMT or DY.     

Effect of flaxseed and wheat flour blends on dough rheology and bread quality

The rheological and baking properties of flaxseed/wheat composite flours were studied. Flaxseed flour was used to replace 50, 100, 150 and 200 g kg^?1 of wheat flour in bread. Farinographic studies showed that water absorption, dough development time and mixing tolerance index increased as the amount of flaxseed flour increased, while dough stability decreased at 100, 150 and 200 g kg^?1 of flaxseed flour substitution. The extensographic energy of dough also decreased at 150 and 200 g kg^?1 flaxseed levels. The addition of increasing amounts of flaxseed flour caused a decrease in extensibility. Doughs containing 100, 150 and 200 g kg^?1 flaxseed flour showed resistance to extension comparable to that of control dough. The specific volume of flaxseed flour breads was similar to that of control bread. Crust L, a, b values of breads with flaxseed flour were lower than those of control bread. Breads with flaxseed flour gave lower crumb L and b values and higher a values than control bread. The sensory properties showed that an acceptable bread could be produced using flaxseed flour up to a level of 200 g kg^?1. Copyright © 2007 Society of Chemical Industry     

Impact of thermostable amylases during bread making on wheat bread crumb structure and texture

Thermostable amylases [Bacillus subtilis α-amylase (BSuA) and B. stearothermophilus maltogenic amylase (BStA)] with different modes of action and impacts on firming properties were added during straight-dough bread making. BSuA continuously degraded the starch fraction during bread making. Its action resulted in larger gas cells than in control bread, but did not change initial firmness. In contrast to BSuA, BStA mainly degraded starch at the end of the baking phase and during bread cooling, which caused little if any impact on bread crumb texture. However, it led to higher initial firmness readings than for the control breads. Neither BSuA nor BStA were inactivated during bread making. The results evidence that starch properties have a large impact on bread crumb structure and initial firmness and are highly influenced by the mode of action of the enzyme.     

Nutritional and functional added value of oat, Kamut, spelt, rye and buckwheat versus common wheat in breadmaking

BACKGROUND: Multicereal/pseudocereal blends based on major and minor seeds with high nutritional profile appear as a promising strategy, hardly explored, to obtain enhanced value grain‐based foods such as bread. RESULTS: In a preliminary stage the suitability of minor/ancient cereals (rye, oat, Kamut^® wheat, spelt wheat) and pseudocereals (buckwheat) was assessed in single (100% of wheat flour replacement) and multigrain (from 20 to 44% of wheat flour replacement) matrices. The research allowed identification of the qualitative (oat, rye, buckwheat) and quantitative (up to 75% of wheat flour replacement) grains in the mixed matrices providing enhancement of nutritional quality (higher protein content, higher mineral content, lower digestible starch, higher viscous fibre content, higher resistant starch content, source of antioxidants) and minimisation of techno‐functional impairment and sensory depreciation of the resulting breads. CONCLUSION: The quality profile of associated mixtures of oat, rye, buckwheat and common wheat flours (20:20:20:40 w/w/w/w) endorsed the proposed grain blend as suitable to make highly nutritious, modern and innovative baked goods meeting functional and sensory standards in terms of nutritional added value (improved dietary fibre fractions, minerals and antioxidant activity, slower starch hydrolysis), palatability (high sensory scores), convenience (extended keepability during storage) and easy handling during processing. Copyright © 2011 Society of Chemical Industry     

High-Legume Wheat-Based Matrices: Impact of High Pressure on Starch Hydrolysis and Firming Kinetics of Composite Breads

The significance of high hydrostatic pressure (HHP) processing on the physico-chemical—techno-functional and firming kinetics—parameters and nutritional properties—nutritional composition and “in vitro” starch digestibility—of highly replaced wheat flour breads by chickpea, pea and soybean flours was investigated, and the power/effectiveness of HHP in partially replacing structural agents (gluten and/or hydrocolloids) was discussed. Incorporation of pressured legume slurries (350 MPa, 10 min) at 42% of wheat replacement into bread formulation provoked a general increase in initial crumb hardness and browning of the crust with a concomitant explicit reduction of moisture, whiteness of the crumb and bread specific volume, but a slower in vitro starch digestibility with prominent formation of slowly digestible starch and resistant starch, compared to their counterparts prepared by using a conventional gluten/carboxymethyl cellulose (CMC)-added breadmaking recipe/process. Pressured breads with no gluten but 3% CMC in the formulation kept higher sensory ratings, softer initial texture and lower firming profiles on ageing than pressured breads with no gluten nor CMC. HHP has proven to be an effective technology to partially replace structuring agents (CMC and/or gluten) in high-legume wheat-based matrices providing sensorially acceptable breads with medium physico-chemical quality profile but enhanced formation of nutritionally relevant starch fractions and slower crumb firming kinetics on ageing.     

Release of free ferulic acid and changes in antioxidant properties during the wheat and rye bread making process

Changes in the free ferulic acid (FFA) contents and antioxidant properties during bread making processes were determined. Experimental breads were produced from whole meal and white wheat and rye flours, and fermented using either baker’s yeast or sourdough starter. Sourdough fermented bread contained the highest content of FFA. Release of occurred mainly during dough fermentation. A further increase in the ferulic acid content in the bread crumb and a decrease in the crust was observed. Total antioxidant properties of sourdough bread, defined as the sum of lipophilic and hydrophilic compound activities, were significantly (p<0.05) higher than for yeast bread. Sourdough bread contained more methanol soluble phenolic compounds, proteins, tocochromanols, and oxidized products of fatty acids than yeast bread. The equilibrium between the anti- and pro-oxidative compound contents resulted in similar antioxidant properties for bread using both types of fermentation, and to results observed for the flour used for baking.     

Investigation of product quality, sensory profile and ultrastructure of breads made from a range of commercial gluten-free flours compared to their wheat counterparts

Bread is a major staple food consumed daily in all parts of the world. A significant part of the human population cannot tolerate gluten, a storage protein found in wheat, rye and barley, and therefore, products made from alternative cereals are required. During this study, the bread-making potential of seven gluten-free flours, wheat and wholemeal wheat flour was compared. Fermentation potential of the different flours was determined, showing that dough development height of gluten-free and wholemeal wheat samples was lower than for wheat and oat flour. Apart from standard bread quality parameters such as loaf-specific volume and physical crumb texture, also water activity and shelf life have been determined. The shelf life of gluten-free breads was reduced compared to wheat bread. Aroma profiles were evaluated by a trained panel. Wheat, oat and wholemeal wheat breads were liked moderately, while the remaining samples had lower liking scores. Crumb grain characteristics were investigated using image analysis, and microstructure was observed by scanning electron microscopy. Overall, only breads produced from oat flour were of similar quality to wheat bread, and the utilization of buckwheat, rice, maize, quinoa, sorghum and teff flours resulted in breads of inferior quality.     

Impact of sodium chloride on wheat flour dough for yeast-leavened products. II. Baking quality parameters and their relationship

BACKGROUND: The traditional use of sodium chloride (NaCl) fulfills various important rheological, technological and sensory properties in the manufacturing of yeast‐leavened products. However, the use of NaCl in food production has been discussed controversially since a high intake of sodium seems to be associated with hypertension. This study investigates the baking quality parameters of wheat breads containing various levels of NaCl (0–40 g NaCl kg^?1flour). RESULTS: Crumb firmness and rate of bread staling decreased with decreasing NaCl levels. A slight increase in loaf volume was observed based on the increased yeast leavening ability resulting from additional NaCl. Higher crumb retrogradation (measured by differential scanning calorimetry) was observed with low NaCl levels. CONCLUSION: The retrogradation effect is based on the theory that NaCl probably leads to Na⁺ inclusion in starch molecules during storage and thus reduces retrogradation. Further, significant (P?0.05) linear relationships (r ≥ 0.829) between Rheofermentometer results, bread volume and crumb firmness were found, suggesting a predictability of bread quality by measurement of gas release. Copyright © 2011 Society of Chemical Industry     

Reducing the glycaemic index and increasing the slowly digestible starch content in gluten‐free cereal‐based foods: a review

Commercially available gluten‐free (GF) cereal‐based foods are generally characterised by a lower nutritional quality than their gluten‐containing counterparts, relatively lower resistant starch (RS) content, along with higher glycaemic index (GI) often being reported. To overcome this nutritional imbalance, extensive research has been conducted to investigate the preparation of a new generation of staple GF products. This review reported the main strategies currently adopted in GF cereal‐based food recipes to formulate products with overall slowly digestible starch properties. They are mainly obtained by the utilisation of alternative ingredients to be incorporated into standard food formulation (including native starch and GF flours) or by technological treatments that may contribute to impact starch digestibility. Considering data from in vitro digestion trials, indications suggested that, aiming to obtain a RS content of about 5% (dry weight) and lowering the in vitro GI values, the minimum high amylose starch (amylose >60%) replacement level in GF bread and cookie formulations should be in the order of 20% by weight of total flours. Overall, with respect to un‐substituted GF foods, two‐ to three‐times higher RS contents, along with a parallel in vitro GI decrease (up to ?50%), were obtained for GF pasta and ready‐to‐eat snacks enriched with legume flours at inclusion levels from 40% to 100% by weight of total flours. The use of flours from pseudocereals (e.g., quinoa, amaranth and buckwheat) with and without sourdough did not always guarantee favourably slowly digestible starch GF foods.     

Effect of high pressure processing on wheat dough and bread characteristics

Microbial, physical and structural changes in high pressured wheat dough were studied as a function of pressure level (50-250 MPa) and holding time (1-4 min). Thereafter, selected conditions of high hydrostatic processing (HPP) were applied to bread dough and the technological quality of the obtained breads was studied. The effect of HPP on wheat dough was investigated by determining microbial population (total aerobic mesophilic bacteria, moulds and yeasts), color and mechanical and texture surface related dough parameters (cohesiveness, adhesiveness, hardness and stickiness). HPP reduced the endogenous microbial population of wheat dough from 10⁴ colony forming units/g (CFU) to levels of 10² CFU. HPP treatment significantly (P < 0.05) increased dough hardness and adhesiveness, whereas treatment time reduced its stickiness. Scanning electron micrographs suggested that proteins were affected when subjected to pressure levels higher than 50 MPa, but starch modification required higher pressure levels. HPP treated yeasted doughs led to wheat breads with different appearance and technological characteristics; crumb acquired brownish color and heterogeneous cell gas distribution with increased hardness due to new crumb structure. This study suggests that high hydrostatic processing in the range 50-200 MPa could be an alternative technique for obtaining novel textured cereal based products.     

Berry fruits-enriched pasta: effect of processing and in vitro digestion on phenolics and its antioxidant activity,bioaccessibility and potential bioavailability

Pasta samples were made by substituting wheat flour (2.5% and 7.5%) for lyophilised raspberry, boysenberry, and redcurrant and blackcurrant. Total polyphenol content showed minimum variation during processing while anthocyanins presented high degradation. In general, the first minutes of cooking showed the major detrimental effect on antioxidant activity. In vitro starch hydrolysis showed the lowest value with the addition of raspberries and boysenberries. During simulated digestion, polyphenols were released from pasta matrix reaching a 2.3- to 4.3-fold increase in bioaccessible polyphenols. Likewise, values observed for reducing power and free radical scavenging activity ranged from a 0.7- to 2.0-fold and 1.6- to 6.8-fold increase in relation to cooked pasta, respectively. In addition, ≈40% of dialysability was observed for scavenging activity. In conclusion, enrichment of pasta with fine fruits is an effective tool to obtain a product with enhanced antioxidant potential.     

Quality of wheat bread enriched with onion extract and polyphenols content and antioxidant activity changes during bread storage

We determined the effect of onion skin extract (OE) on the quality of wheat bread: contents of total polyphenols and flavonols, antioxidative activity and their changes during storage. With increasing doses of OE to bread, contents of total polyphenols and flavonols and the antioxidative activity were observed to increase. A higher total flavonol content was found in bread with 1% OE stored at 24 °C than in bread stored at 4 °C. There was no such relationship in the bread with 0.5% OE. Changes in flavonols content during storage for 72 h were insignificant. No clear trends of change in the antioxidant activity could be found at storage of breads. The addition of OE caused no changes in the yield and volume of bread and contributed to a decreasing value of L* parameter and to increasing values of ΔE, a* and b* parameters of bread crumb colour. Consumers accepted the smell and taste of bread with the addition of OE to a lesser extent than the control bread. Bread with 1% OE the taste was rated very low. Consumers rated the taste of bread with 1% OE low because it was bitter. Breads enriched with OE had a proper external appearance as well as crumb and crust features. Roasted onion aroma and taste were perceptible in the obtained breads. A bitterness was perceptible in breads baked with the addition of 1% of OE.