共查询到19条相似文献,搜索用时 100 毫秒
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荧光试剂二(1-H-苯并三唑)乙二酮缩氨基硫脲的合成及其在蛋白质测定中的应用 总被引:2,自引:1,他引:1
以苯并三唑、乙二醛、氨基硫脲为基本原料 ,合成了荧光试剂二 (1 H 苯并三唑 )乙二酮缩氨基硫脲席夫碱。通过对试剂进行红外光谱、元素分析确证了试剂的结构 ,并利用荧光分析法研究了它在蛋白质测定中的应用。在pH为 8 0的磷酸缓冲溶液中 ,该试剂在 394nm处 (λex=342nm)发射荧光。实验表明 ,其荧光强度随着蛋白质的加入明显增强 ,据此建立了测定痕量蛋白质的新方法。在最佳实验条件下 ,对牛血清白蛋白、人血清白蛋白的测定 ,其线性范围分别为 0~ 1 0μg·mL-1 ,0 5 0~ 1 0 μg·mL-1 ,检测限分别为 6 3× 1 0 -3 ,1 2× 1 0 -3 μg·mL-1 。 相似文献
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六羰基钨催化制备聚苯乙炔及其荧光性能的研究 总被引:1,自引:0,他引:1
用W(CO) 6 催化体系聚合苯乙炔 ,以高产率获得较高相对分子质量的聚苯乙炔。使用UV、IR、GPC等分别对聚苯乙炔结构进行表征。采用不同波长的激发光对聚苯乙炔荧光性能进行了详细研究 ,结果表明 :当聚苯乙炔用 31 0nm激光激发时 ,呈现 3个荧光发光峰 ,峰值分别在348、396和 61 0nm处 ,与其溶液的紫外吸收有类似的光谱曲线特征。荧光峰在 348nm处的荧光 ,当聚苯乙炔质量浓度大于 0 0 5g L时 ,呈现荧光猝灭效应 ;在聚苯乙炔质量浓度为 1 0 - 4g L时 ,呈现出最大的荧光发光量子效率。当激发光波长低于 2 70nm和高于 340nm时 ,348nm波长荧光消失 ;荧光峰在 396nm处的荧光 ,当聚苯乙炔的质量浓度为 1 0 - 2 g L时 ,呈现最大的发光量子效率 ,聚苯乙炔的质量浓度增大或降低 ,其发光强度均呈现减弱现象 ;荧光峰在 61 0nm的荧光 ,在聚苯乙炔质量浓度为 1 0 - 4g L时 ,呈现最大的发光量子效率。荧光性能研究结果证明这种催化剂获得的聚苯乙炔具有多种异构链结构。 相似文献
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The fluorescence properties of a chiral polymer based on optically active polybinaphthyls were studied in tetrahydrofuran solution. One‐photon excited fluorescence of the polymer was located in the range of ~ 596 nm and the corresponding lifetime was ~ 4.38 ns. From the excitation spectra and emission spectra excited at 800 nm, the upconversion fluorescence emission was observed. When excited by using 800 nm fs laser pulses with different input irradiances, the peak fluorescence intensity of the solution provides square dependence with the input irradiance power, giving an evidence for two‐photon excited fluorescence. Furthermore, open aperture Z‐scan measurements were performed at different irradiation intensities to confirm two‐photon absorption property of the solution at 800 nm excitation. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012 相似文献
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聚苯砜对苯二甲酰胺(PSA)纤维在防护领域具有广泛的应用,笔者在其纺丝溶液中引入苝系荧光功能材料POSS-PDI-POSS,通过静电纺制备了PSA纤维膜。研究了助纺剂聚丙烯腈(PAN)、共溶剂氯仿、荧光功能材料的引入对PSA溶液流变、电导率和纺丝成形性能的影响,发现在PAN添加量为3%(w)、氯仿添加量为6%(w)和0.4%(w)的POSS-PDI-POSS时可制备纤维直径集中分布在250~600 nm的纤维膜,该纤维膜可发射550 nm的黄绿色荧光(490 nm光激发)和发射580 nm的红色荧光(530 nm光激发)。 相似文献
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D
M Reynolds 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2002,77(8):965-972
The chemical and biochemical oxygen demand values of a number of synthetic and wastewater samples were determined using fluorescence spectroscopy. Treated and untreated sewage samples were obtained from a local sewage treatment works while synthetic samples were analysed before, during, and after treatment via a rotating biodisc contactor. Fluorescence intensities were normalised using the water Raman signal as an internal standard and corrections applied to take into account the attenuation effects caused by the sample matrix. The fluorescence emission spectra (λexc = 280 nm) of synthetic and sewage samples were very similar in that two main fluorescence bands centred around 350 nm and 440 nm were observed in all samples. Normalised fluorescence data, centred at 350 nm, correlate well with corresponding BOD, COD and TOC values (R2 values ranging between 0.93 and 0.98). Using BOD, COD and TOC data the fluorescence at 350 nm and 440 nm can be apportioned to biodegradable and non‐biodegradable dissolved organic matter respectively. The findings of this research show that fluorescence data can be used to quantify oxygen demand values (chemical and biochemical) and total organic carbon values. Furthermore, the fluorescence spectral response can be apportioned to biodegradable (BOD) and non‐biodegradable (COD ? BOD) dissolved organic matter. The potential of using fluorescence spectroscopy as a possible tool for real‐time monitoring of sewage wastes is discussed. © 2002 Society of Chemical Industry 相似文献
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用 0 .3m mol· L- 1的十二烷基苯磺酸钠提取钝顶螺旋藻中的藻蓝蛋白和别藻蓝蛋白 ,再在硅藻土5 4 5柱上分级洗脱 ,进一步经 DEAE-纤维素柱纯化 ,藻蓝蛋白纯度达到了 4 .1(指可见区最大吸收与 2 80 nm处之比 ) ,最大吸收峰在 6 19nm,室温荧光发射峰在 6 4 3nm。别藻蓝蛋白纯度达到了 4 .7(指可见区最大吸收与 2 80 nm处之比 ) ,最大吸收峰在 6 5 2 nm,室温荧光发射峰在 6 6 7nm 相似文献
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Ikeda S Kubota T Wang DO Yanagisawa H Umemoto T Okamoto A 《Chembiochem : a European journal of chemical biology》2011,12(18):2871-2880
A binary photocontrolled nucleic acid probe that contains a nucleotide modified with one photolabile nitrobenzyl unit and two hybridization-sensitive thiazole orange units has been designed for area-specific fluorescence imaging of RNA in a cell. The synthesized probe emitted very weak fluorescence regardless of the presence of the complementary RNA, whereas it showed hybridization-sensitive fluorescence emission at 532 nm after photoirradiation at 360 or 405 nm for uncaging. Fluorescence suppression of the caged probe was attributed to a decrease in the duplex-formation ability. Caged fluorescent nucleotides with other emission wavelengths (622 and 724 nm) were also synthesized in this study; they were uncaged by 360 nm irradiation, and emitted fluorescence in the presence of the complementary RNA. Such probes were applied to area-specific RNA imaging in a cell. Only probes in the defined irradiation area were activated by uncaging irradiation, and subnuclear mRNA diffusion in a living cell was monitored. 相似文献
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本文以苯甲酸为原料,经酰氯化生成苯甲酰氯,再与间苯二酚反应生成中间体2,4-二羟基二苯甲酮,产率79.4%。然后以2,4-二羟基二苯甲酮为封闭基,对位酯为重氮组分合成了黄、橙、红和蓝色4只新结构的活性染料,并用质谱对其结构进行了表征。用合成的染料对棉纤维染色,测定了染料的上染率和固色率,黄色染料染色性能较差。与未染色的棉纤维相比,染色后棉纤维的紫外线透过率大大降低。未染色纤维UPF值为1.3,经过黄、橙、红和蓝色染料染色的棉纤维的UPF值分别为6.1、16.1、16.0、18.5,除黄色外都达到了很好的抗紫外线效果。 相似文献
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报道了具有典型D-A-D型共轭结构的反式2,5-二氰基-1,4-二(4′-甲氧基苯乙烯基)苯(DCHO)的合成。用核磁、红外和元素分析进行了表征。测试了紫外吸收光谱、单光子荧光光谱、双光子荧光光谱及双光子吸收截面。在800 nm的飞秒脉冲激光激发下,标题化合物发出很强的绿色上转换荧光。化合物2,5-二氰基-1,4-二(4′-甲氧基苯乙烯基)苯的最大吸收波长、单光子发射波长、最大双光子荧光波长、荧光量子产率、双光子吸收系数及双光子吸收截面分别是393 nm、470 nm、475 nm、0.12、0.8 cm/GW、270 GM。这些数据表明,对位甲氧基的D-A-D型化合物具有较好的双光子吸收性能,DCHO是双光子荧光显微与成像应用的一个良好的候选材料。 相似文献