复方中草药添加剂对肉鸡肉质的影响

选取1日龄AA肉鸡180只,随机分为4个组,每个组设3个重复,每个重复15只鸡。A为对照组,饲喂基础日粮;B、C、D为试验组,分别在基础日粮中添加1％的复方中草药添加剂Ⅰ、Ⅱ、Ⅲ。结果表明,3种复方中草药添加剂都能不同程度地提高肉鸡的胸肌率、腿肌率、瘦肉率、系水力（P〈0．05）,降低腹脂率、滴水损失和烹煮损失（P〈0．05）,改善肉色（P〈0．05）,使肌纤维变细（P〈0．05）。复方中草药添加剂对肉鸡屠宰率、全净膛率、半净膛率以及鸡肉pH值影响不显著（P〉0．05）,但有增加的趋势。3种复方中草药添加剂在改善肉质方面以复方中草药添加剂Ⅲ效果最好。     

几种酵母菌添加剂对奶牛产奶量及乳成分的影响

将54头高产中国荷斯坦奶牛随机分为6组，1组基础对照组，5组（A，B，C，D，E）试验组，试验组在基础日粮的水平上添加15g不同型号的酵母菌制剂，预试期2周，正试期为6周。结果表明：不同型号的酵母菌对奶牛产奶量和奶成分有不同程度的影响，其中C和E组奶牛的产奶量分别比对照组有所增加，B和D组的产奶量与对照组相比增加显著（P〈0．05），A组则有所下降；不同型号的酵母菌制剂对牛乳成分也有不同程度的影响，试验组B，D，E组乳脂日产量比对照组有增加，并且差异显著（P〈0．05）；E组乳脂率增加，差异显著；蛋白质日产量除A组无显著性差异（P〉0．05），B，C，D，E组相比对照组都有所增加，其中C和E组相比对照组，差异显著（P〈0．05）；试验组的乳糖、非脂固形物产量与对照组没有显著差异（P〉0．05）；各试验组的所生产牛奶的比重、冰点、酸度与对照组无显著差异，但试验组的酸度除D组外均比对照组有所提高，乳中体细胞数均比对照组低，其中酵母菌C和D能显著减少乳中体细胞数（P〈0．05）。     

烟酸铬对肉鸡生产性能、胴体品质及和血液生化指标的影响

选择1日龄艾维菌商品肉鸡360羽，随机分成3组，每组3个重复，每个重复40羽，试验期6周，研究了在日粮中分别添加0，0．2，0．4mg/kg铬量的烟酸铬对肉鸡生产性能，胴体品质及血液生化指标的影响，结果表明：在0－6周龄的肉鸡日粮中添加不同水平的烟酸铬对肉鸡生产性能无显著影响（P＞0．05）。但添加烟酸铬可明显改善胴体品质，显著提高胸肌率（P＜0．05）、降低腹脂率（P＜0．01），对肝脂率、屠宰率，净膛率却无显著影响（P＜0．05），及甘油三酯的含量（P＜0．01），并显著提高血清中高密度脂蛋白－胆固醇的质量浓度（P＜0．05）。     

豆油能量对朗德鹅超饲养前期生长性能、血清参数及其相关激素水平的影响研究

用180只1日龄初生重大致相同的朗德鹅为试验对象，公母各90只。通过添加不同的豆油水平，设计高中低三种不同能量水平（Ⅰ：13．38MJ／kg、Ⅱ：12．13MJ／kg、Ⅲ：10．87MJ／kg）的日粮，粗蛋白水平均为20％，把公母各90只朗德鹅随机各分为试验Ⅰ组，试验Ⅱ组，试验Ⅲ组，每组30只，共计6组，分栏饲养70d。实验朗德鹅每周进行称重，于21日龄、70日龄两个阶段进行屠宰实验，屠宰前采集血液进行血清参数和血清激素水平检测，结果发现：（1）0～70日龄阶段，在保证蛋白质需要的基础上，采食量、料重比和日粮能量成负相关；要达到较好的生长效果，此阶段公鹅需要的最佳能量（12．13MJ／kg）要低于母鹅所需最佳能量（13．38MJ／kg）；（2）采用豆油形式添加的能量对不同能量组的血清中TG、LDL、HLDL影响差异不显著（P〉0．05）；70日龄时血清AⅡ含量高低和动物的增重情况和机体脂肪含量比较吻合；（3）豆油形式添加的能量对腹脂率的影响不显著（P〉0．05），对肌间脂肪影响显著（P〈0．05）；（4）朗德鹅生长前期血清IGF—Ⅰ的含量和生长速度呈正相关，血清GH的含量和能量的高低呈正相关。     

大豆异黄酮对樱桃谷鸭生长和屠宰性能的影响

选用健康1日龄樱桃谷SM2商品代雏鸭128只,随机分为4组,公、母各半.对照组饲喂基础日粮,试验I、Ⅱ、Ⅲ组日粮是在基础日粮中分别添加5、10和15 mg/kg的大豆异黄酮,于42日龄屠宰.结果表明:①试验组公鸭、母鸭每周平均体重均显著高于或高于对照组,试验Ⅱ组的平均体重显著高于其他各组(P＜0.05).大豆异黄酮对樱桃谷鸭母鸭和公鸭的生长具有明显的促进作用,而且其作用效果呈现剂量效应,以10 mg/kg添加水平效果最为明显.②试验Ⅱ组母鸭的屠宰率和胸肌率高于其他各组(P＞0.05);母鸭和公鸭的腹脂率均显著低于其他各组(P＜0.05).试验Ⅲ组母鸭的腿肌率显著高于其他各组(P＜0.05).试验组间母鸭的皮脂率差异不显著(P＞0.05);但均显著低于对照组(P＜0.05),试验Ⅲ组母鸭的皮脂率最低.试验Ⅲ组公鸭的屠宰率、胸肌率和腿肌率高于其他各组(P＞0.05),试验I、Ⅲ组公鸭的腹脂率也显著低于对照组(P＜0.05),试验Ⅱ组公鸭的皮脂率显著低于其他各组(P＜0.05).大豆异黄酮发挥了降脂作用,且以10 mg/kg的作用最为明显.     

鱼油替代菜籽油对蛋鸡生产性能及脂质代谢的影响

试验共设四个组，对照组含3％菜油，试验组分别含2％菜油＋1％鱼油、1％菜油＋2％鱼油和3％鱼油，研究日粮鱼油替代菜籽油对蛋鸡产蛋性能、蛋品质和脂质代谢的影响。结果表明，蛋黄中ALA、DHA含量以2％鱼油添加组最高，分别比对照组提高32．88％（P〈0．05）和22．49％（P〉0．05），EPA含量以1％鱼油添加组最高，比对照组提高13．48（P〈0．05）；添加鱼油组蛋黄TC含量升高（P〉0．05），TG变化不明显，MDA含量升高（P〉0．05）；血清TC含量升高，以3％鱼油添加量组最高，比对照组提高3．55％（P〈0．05），2％、3％鱼油添加组TG含量降低，分别比对照组下降12，52％（P〉0．05）和51．18％（P〈0．05）；血清HDL—C含量升高，LDL—C含量降低，SOD、MDA含量有所降低，蛋重以2％和3％鱼油添加组显著低于对照组（P〈0．05），蛋壳强度分别比对照组提高13．79％（P〉0．05）、41．72％（P〈0．05）和32．37％（P〉0．05）。研究表明，蛋鸡日粮中添加鱼油可以提高ω-3PUFA在蛋黄中的沉积，影响机体的脂质代谢，同时伴随脂质过氧化物合成增加，但对产蛋性能无明显影响。综合各种指标，蛋鸡日粮中以2％的鱼油添加量为佳。     

日粮不同氟水平对生长猪微量元素沉积的影响

96头杜长大三元杂交猪，随机分为4组，第1组为对照组，饲喂基础日粮（含氟量37．39mg／kg），第2、3和4组分别饲喂含氟（以NaF形式添加）量为100，150，200mg／kg的日粮，研究日粮中不同氟水平对猪生长性能以及不同组织器官铁、铜、锌和锰沉积的影响。实验结果表明，第3、4组生长猪日增重与对照组相比分别降低了6．25％（P〈0．05）和8．22％（P〈0．05），料重比分别升高了7．37％（P〈0．05）和8．07％（P〈0．05）。而第2组猪的生长性能与对照组相比均无显著差异（P〉0．05）。另一方面，实验结果表明第3、4组氟的添加与对照组相比除显著增加了生长猪肾锌的沉积外（P〈0．05），均显著降低了大多数组织器官铁、铜、锌和锰的沉积（P〈0．05）。而第2组氟的添加量对于各微量元素在组织器官中的沉积与对照组相比均无显著差异（P〉0．05）。     

复合酶制剂在肉鸡生产中的应用

540只7日龄萨索肉鸡随机分为6个处理组，每个处理组设6个重复，每个重复15只鸡，分别饲喂A、B、C、D、E、F6种日粮，A组为正对照组，B组为负对照组，C～F组为在B组基础上分别添加0．012％、0．015％、0．018％和0．021％溢多利浓缩微丸酶，A组代谢能较其他各组高0．42MJ／kg。结果表明，与B组相比，添加0．015％的溢多利浓缩微丸酶可显著提高2～7周龄肉仔鸡日增重和期末体重（P〈0．05），趋于提高采食量并降低料肉比（P〉0．05）。A组可显著提高料肉比（P〈0．05），其他各项指标与B组无显著差异（P〉0．05）。低能量日粮添加复合酶制剂可极显著提高小肠内淀粉酶活性（P〈0．01），也不同程度提高了小肠胰蛋白酶活性。低能量日粮和添加酶制剂均可提高血糖浓度和血清尿素氮水平。     

高F值寡肽酸乳饮料的开发与功能评价研究

利用复合酶水解玉米蛋白粉制得的高F值寡肽（F值为21．7）进行酸乳饮料配方与功能特性研究。结果表明，饮料最佳工艺配方为：添加高F值寡肽3％，白砂糖8％，柠檬酸0．15％；试验组与对照组相比，高、中剂量组缺氧存活时间分别提高了48％（P〈0．01）和33％（P〈0．05）；游泳时间分别提高了64％（P〈0．01）和38％（P〈0．05），肝糖原含量分别为对照组的2．75倍（P〈0．001）和1.88倍（P〈0．01）；游泳前后小鼠血乳酸含量分别与对照组相比降低了63．5％（P〈0．01）和35．6％（P〈0．05），证明了玉米高F值寡肽酸乳饮料的抗缺氧和抗疲劳功能。     

Diludine对肉仔鸡的生产性能及胴体品质的影响

选用 1日龄AA肉用雏鸡 30 0只 ,随机分成 5组。按Diludine添加水平的不同分为 5个不同处理组 ,即 0 (对照组 )、5 0、10 0、15 0、2 0 0mg/kg。研究结果表明 :Diludine添加水平为 10 0和 15 0mg/kg时 ,均能显著提高日增重、饲料转化率 (P <0 .0 5 ) ;Diludine添加水平为 5 0、10 0和 15 0mg/kg时 ,均能提高肉仔鸡屠宰率、半净膛率、全净膛率、腿肌率 ,降低腹脂率 ,且不同程度地改善胴体品质。但对腹脂率来说以 2 0 0mg/kg组的腹脂率最低 ,比对照组下降了 12 .4 5 % ;其次是 15 0mg/kg组 ,比对照组下降了 12 .0 3%。     

法国朗德鹅肉用性能及营养成分分析研究

选择引进的纯种法国朗德鹅进行饲养,在85日龄时,选体质强壮、健康无病、体重在4.0～4.3千克的朗德鹅70只进行屠宰取样,对朗德鹅肉用性能、胸腿肌化学组成、氨基酸含量、皮下脂肪和肾脂肪的一般化学特性及主要高级脂肪酸组成等肉脂基本性状进行分析测定,并与我国其他地区鹅肉脂作一比较.测定结果表明:朗德鹅具有较好屠宰性能、肌肉的干物质含量为26.39%,粗蛋白为19.98%,粗脂肪为5.68%,灰分2.33%,Ca0.029%,P0.15%;朗德鹅肌肉的各种必需氨基酸含量(除色氨酸外)均较高,特别是对人类具有特殊意义的赖氨酸含量高达6.54%,与风味关系密切的谷氨酸高达16.34%.朗德鹅脂肪的不饱和脂肪酸含量高达64.80%.脂肪化学特性:碘价70.86,酸价0.4933,皂化价195.34.得出结论:朗德鹅肌肉富含呈风味物质、必需氨基酸含量丰富、脂肪含量低并且不饱和脂肪酸含量高,是人类较好的动物肉食原料.     

Composition and estimation of intramuscular and subcutaneous fatty acid composition in Spanish young bulls

Sixty Pirenaica yearling intact bulls were raised and slaughtered at 519±50kg live weight and 371±18 days of age. Fatty acid composition of intramuscular lipids in longissimus dorsi muscle and subcutaneous fat were determined by capillary gas chromatography. Carcass conformation and fatness, carcass weight, ether extractable intramuscular fat percentage, marbling, dorsal fat thickness, and ultrasonic parameters were recorded to study the relationship between these traits and fatty acid profile. Lipids of subcutaneous adipose tissue had higher monounsaturated fatty acids (MUFA) percentage (49% vs. 33%) but lower saturated fatty acids (SFA) (51% vs. 46%) and polyunsaturated fatty acids (PUFA) (16% vs. 5%) percentages than intramuscular fat depots (p<0.001). Fatter carcasses had higher MUFA percentage and lower PUFA percentage (p<0.01) in intramuscular fat. Carcass weight and conformation would contribute to explain the fatty acid profile of subcutaneous fat (p<0.05). Ultrasound readings reflected the effect of fatness on fat composition.     

Fatty acid composition of adipose depots of suckling lambs raised under different production systems

Forty-nine male Talaverana-breed suckling lambs slaughtered at 14 kg live weight were used to study the fatty acid composition of adipose depots of lambs raised under different management systems (pasture or drylot) and diets (maternal milk with or without a concentrate supplement). Drylot lambs displayed a higher fatness score, in addition to greater dorsal fat thickness; these parameters corresponded with a greater percentage of total fat at dissection. Addition of concentrate to the diet did not modify carcass fatness. Drylot lambs presented higher proportions of oleic acid (C18:1) and monounsaturated fatty acids (MUFA) and lower linolenic acid (C18:3) values in subcutaneous and intramuscular fat (muscle longissimus thoracis). The subcutaneous fat of these lambs thus displayed a higher n-6/n-3 PUFA ratio (C18:2/C18:3) than that of pasture-raised ones. Concentrate in the diet of both pasture and drylot lambs resulted in higher linoleic fatty acid (C18:2) and PUFA values and a greater PUFA/SFA ratio in intramuscular fat than the corresponding values of lambs which consumed maternal milk alone. Loin displayed a higher percentage of subcutaneous and intramuscular chemical fat than leg. Compared with intramuscular leg fat (muscle quadriceps femoris), intramuscular loin fat (muscle longissimus thoracis) had lower oleic acid (C18:1) and MUFA values, higher PUFA values and a higher PUFA/SFA ratio, due to greater linolenic acid (C18:3) levels.     

Halothane genotype and pork quality. 1. Carcass and meat quality characteristics of three halothane genotypes

The carcass and meat quality characteristics of three halothane genotypes in pigs were evaluated. Sixty crossbred Landrace×Large White pigs (NN=25, Nn=19, nn=16) of ±86 kg live weight were slaughtered, the carcasses chilled for 24 h at 2°C, certain carcass and meat quality traits determined and the shoulder and leg cuts deboned and cut into primal cuts. Dressing percentage of the Nn pigs was higher (P<0.05) than that of the NN and nn pigs. Carcass length was the longest (P<0.05) for the nn pigs. Midline fat measurements as well as measurements at the 2nd-3rd last rib (45 mm from the midline) indicated that the nn pigs had less fat (P<0.05) and a larger eye muscle width (P<0.05), depth (P<0.001) and area (P<0.001) than the NN or Nn pigs. This resulted in the nn pigs having the highest (P<0.05) percentage predicted carcass lean content. Fat, bone and lean yield of the shoulder identified the nn pigs with the least bone (P<0.05) and fat (P<0.001) and the highest lean yield (P<0.05), expressed as a percentage of total shoulder weight. Yield from the legs identified the nn pigs as having the lowest fat (P<0.001) and highest lean (P<0.05), expressed as a percentage of total leg weight. The primal lean cuts (topside, silverside, thickflank and rump) from the legs showed that the nn pigs had the highest values (P<0.05) for lean as a fraction of cold carcass weight. This resulted from the higher weight of these cuts (P<0.05) in the nn pigs. Calculation of bone yield in the legs showed that the femur weights were highest (P<0.05) for the Nn pigs, also when expressed as percentage of leg weight (P<0.05) and as a fraction of cold carcass weight (P<0.05). Comparison of meat quality traits show that the nn pigs had poor quality with regard to pH(45) (P<0.001), pH(24) (P<0.05), drip loss (P<0.001) and reflectance values (P<0.05).     

Effect of artificial rearing on lamb welfare and meat quality

Twenty male Comisana lambs were equally divided into two groups. Ten subjects were artificially reared, 10 others were ewe reared and used as control. Unseparated control animals displayed increased cellular immune response to a percutaneous injection of phytohemagglutinin (P<0.001). During the open field test, ewe-reared lambs showed a higher number of flight attempts (P<0.01). Carcass yield were higher for artificially reared animals (P<0.05). Conversely, legs of ewe-reared animals had significantly more fat (P<0.05) and a higher content of saturated fatty acids (P<0.001). Polyunsaturated fatty acid content was higher in artificially reared lamb meat (P<0.01), although the ratio n-6/n-3 was well above the recommended values. The triangle test showed a significant difference in sensory analysis between the two products (P<0.05). Finally, for both rearing systems legs without subcutaneous fat showed a higher percentage of polyunsaturated fatty acids and a corresponding lower content of saturated fatty acids (P<0.001).     

Effects of weight at slaughter and sex on the carcass characteristics of Florida suckling kids

The effect of slaughter weight and sex on some carcass traits of suckling kids of the Florida breed was evaluated. A total of 60 kids (30 male and 30 female), fed exclusively on milk replacers, were slaughtered at 7-8kg (group 1), 10-11kg (group 2) or 14-15kg (group 3) of liveweight (mean weights of 7.6kg, 10.8kg and 14.4kg, respectively). Higher slaughter weights decreased the percentage of subproducts (blood, skin, head, feet) and internal organs (lungs+traquea, heart, liver, spleen, thymus) but significantly increased the percentage of intestine and fat depots (omental fat and mesenteric fat). Higher slaughter weights also increased carcass measures (L 40.5 vs 49.1; F 22.5 vs 25.9; G 10.4 vs 14.2; Wr 10.1 vs 13.9; Wth 8.0 vs 10.5; Th 16.5 vs 199; B 32.3 vs 42.4; PT 41.5 vs 50.8), compactness carcass index (96.6 vs 152.3) and compactness leg index (27.5 vs 44.1). Sex only significantly affected the percentages of feet, internal organs, omental fat, measure L, carcass compactness index and hind limb compactness index. The meat colour and fat colour were mainly scored as pale and white respectively in the carcasses of the lightest animals, whereas heavier kids were scored as pink and cream. Slaughter weight also influenced significantly the carcass fatness (score 1 in lightest kids and 2 or 3 in heavier ones). There were no significant (p>0.05) differences between slaughter weight group and sex in dressing percentages. Percentages corresponding to the long leg, back and neck (30-33%, 18-19% and 8-10%, respectively) decreased when the slaughter weight increased, whereas the ribs (23-25%) and the flank (10-11%) increased slightly. The carcasses comprised 57-58% muscle, 22-25% bone, 5-6% subcutaneous fat and 9-12% intermuscular fat. The percentage muscle stayed the same with increasing slaughter weight, whereas the bone decreased and the fat increased. The carcasses of the heavier females contained less lean and more fat than the males. The bone percentage was significantly (p<0.05) lower in the females and the carcass fat percentage was significantly (p<0.05) higher than in the males.     

Effect of the Iberian×Duroc reciprocal cross on productive parameters, meat quality and lipogenic enzyme activities

The aim of this study was to investigate the effect of the Iberian×Duroc reciprocal cross on: (i) productive parameters, (ii) physico-chemical traits of three muscles with different oxidative patterns (Longissimus dorsi, Biceps femoris and Psoas maior) and (iii) lipogenic enzyme activities (glucose-6-phosphate dehydrogenase and malic enzyme) in subcutaneous adipose and muscle tissues. Fourteen Duroc-sired (Duroc♂×Iberian♀) and 14 Iberian-sired castrate male pigs (Iberian♂×Duroc♀) were selected at weaning and were reared until 235 days of age. Iberian-sired pigs had significantly higher weight at slaughter (147.7kg vs. 138.8kg, p<0.05) as well as a greater ham and foreleg weight than Duroc-sired pigs. The fatty acid composition of subcutaneous adipose tissue of Duroc-sired pigs showed a higher percentage of C18:0 (13.21% vs. 14.34%, p<0.05) and a lower percentage of monounsaturated fatty acids (47.79% vs. 46.73%, p<0.05) compared to that from Iberian-sired pigs. Contrary to productive parameters, there were no noticeable differences between reciprocal cross in parameters defining meat quality, although there was a clear muscle effect on such parameters with this effect being significant for most of the traits. No differences were found between reciprocal crosses for glucose-6-phosphate dehydrogenase and malic enzymes activities in both muscular and subcutaneous adipose tissues. Lipogenic enzyme activities were considerably higher in subcutaneous adipose tissue compared to muscles. Glucose-6-phosphate dehydrogenase activity did not differ (p>0.05) between muscles, whereas malic enzyme activity was higher (p<0.05) in Psoas maior compared to Biceps femoris and those were higher than in Longissimus dorsi, which was consistent with the positive correlations (p<0.05) found between malic enzyme activity and traits defining oxidative metabolic type. On the contrary, negative correlations (p<0.05) were found between malic enzyme activity and intramuscular fat content, which could suggest that there are differences among muscles in the ability of depositing fatty acids from other tissues.     

大豆活性肽对肥胖小鼠降脂作用的研究

本实验采用大豆活性肽对ICR小鼠进行实验,分为正常对照组、高脂模型组、大豆肽剂量组,灌胃剂量分别为200、400、800 mg/kg低、中、高三个剂量,通过分析小鼠体重及血脂的变化分析了大豆活性肽对高脂诱导引起的小鼠肥胖和血脂代谢异常的影响。试验结果表明,高脂模型组小鼠在灌胃高剂量的大豆活性肽后,其体重、Lee’s指数、摄食量、肝脏及脂肪组织重量均显著降低（P<0.05）;小鼠血清中总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）含量显著下降（P<0.05）,而高密度脂蛋白胆固醇（HDL-C）显著性升高（P<0.05）;小鼠肝脏中TC含量显著下降（P<0.05）,而粪便中TC含量显著增加（P<0.05）。与正常对照组相比,高剂量组的小鼠体重、Lee’s指数、摄食量、肝脏重量、脂肪系数、TC、TG、LDL-C,HDL-C均无显著性差异（P>0.05）,而粪便中TC含量显著增加（P<0.05）。肝脏组织切片结果表明,大豆活性肽能减轻肝脏的脂肪变性程度,且高剂量组的改善效果最明显。因此,大豆活性肽具有很好的降脂作用。     

梭鱼脂肪及脂肪酸成分分析和评价

为了解梭鱼的应用价值,对江苏沿海滩涂池塘养殖梭鱼（均质量（445.8±26.9）g）6 种组织（鱼头、鱼皮、背肌、腹肌、肝脏、腹脂）的脂肪分布、性质、脂肪酸组成进行分析和评价。索氏提取法测定组织中脂肪含量,滴定法测定脂肪的碘值和酸价,气相色谱-质谱法分析脂肪酸的组成。结果表明：梭鱼体内各组织脂肪分布差异显著（P＜0.05）,其中背肌和腹肌中脂肪含量分别为3.66%、10.16%;各组织脂肪的不饱和程度较高,碘值为90～106 g/100 g;各组织脂肪酸价较高,介于4～9 mg KOH/g之间;梭鱼脂肪中C22∶6n 3和C20∶5n 3含量丰富,品质好;鱼头、背肌、腹肌中饱和脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸构成比例约为1∶1.5∶（0.7～0.8）,n-3/n-6多不饱和脂肪酸比值在0.7～0.8之间,组成合理;肝脏和腹脂中脂肪含量丰富,其中含有大量的n-3和n-6多不饱和脂肪酸,因此梭鱼的内脏也具有较好的可利用前景。     

苏尼特羊脂肪组织中脂肪酸沉积机制的研究

本文以12月龄苏尼特羊不同脂肪组织(皮下脂肪、尾部脂肪、肾脏脂肪)为试验对象,利用气相色谱-质谱(GC-MS)技术和实时荧光定量PCR技术研究不同脂肪组织中脂肪酸含量和脂肪代谢相关基因mRNA表达量,同时分析脂肪代谢相关基因与脂肪酸间相关性。结果表明,苏尼特羊脂肪组织中脂肪酸以棕榈酸、硬脂酸和油酸为主。肾脂中硬脂酸的含量显著高于尾脂(p<0.05);尾脂中油酸含量显著高于皮脂和肾脂(p<0.05)。不同脂肪组织间,肾脂中硬脂酰辅酶A去饱和酶(SCD)、脂蛋白脂肪酶(LPL)和脂肪酸合成酶(FASN)基因mRNA表达量显著高于皮脂和尾脂(p<0.05);皮脂中肉碱棕榈酰转移酶(CPT1)和乙酰辅酶A羧化酶(ACC)基因mRNA表达量显著高于肾脂(p<0.05)。通过相关性分析,共轭亚油酸与FADS1、LPL和SCD呈显著正相关(p<0.05);二十碳五烯酸(C_20:5)与FADS1和LPL间呈显著正相关(p<0.05),与ACC呈显著负相关(p<0.05)。由此可知,调控脂肪酸沉积相关基因的表达对脂肪酸沉积的方向和部位有明显的影响。