Deep Frying Performance of Enzymatically Synthesized Palm-Based Medium- and Long-Chain Triacylglycerols (MLCT) Oil Blends

The main aim of this work was to assess the frying strength of the enzymatically synthesized palm-based medium- and long-chain triacylglycerols (MLCT) oil with the aid of different antioxidants under deep-frying conditions. Palm-based MLCT oil in the presence of synthetic or natural antioxidants showed significantly better (P < 0.05) thermal resistance and oxidative strength than refined, bleached, and deodorized (RBD) palm olein throughout the five consecutive days of frying. Rancimat induction period, free fatty acid content, anisidine value, E^\text1% _1\textcm E^{{\text{1\% }}} _{1{\text{cm}}} at 232 and 268 nm, color, percentage of oil uptake, and viscosity measurement can be used as oil quality parameters to indicate the degree of oil deterioration under continuous stressed frying conditions. No significant changes (P > 0.05) in the saturated/unsaturated fatty acids ratio across frying periods indicated good oxidative stability of the palm-based MLCT oil. Due to the polarity of medium- and long-chain triacylglycerols in palm-based MLCT oil, total polar compounds determination may not be a suitable oil quality measures. Sensory evaluation of fried chips showed no significant differences (P > 0.05) between chips fried in RBD palm olein and palm-based MLCT oil over the 3-month storage period.     

Enzymatic Synthesis of Medium- and Long-Chain Triacylglycerols (MLCT): Optimization of Process Parameters Using Response Surface Methodology

The main objective of this study was to understand the effects and relationship amongst four factors, which are reaction temperature, reaction time, enzyme load, and substrate mole ratio with the purpose of producing healthy functional cooking oil for long-term dietary treatment. Lipozyme RM IM lipase-catalyzed esterification of medium- and long-chain triacylglycerols (MLCT) from glycerol and mixtures of capric and oleic acid was optimized using response surface methodology (RSM) with a five-level, four-factorial design. Reaction temperature, reaction time, and substrate mole ratio strongly affected MLCT synthesis (P < 0.01). However, enzyme load did not have a significant (P > 0.01) effect on MLCT yield. Comparison between predicted and experimental value from central composite rotatable design optimization procedures revealed good correlation, implying that the reduced cubic polynomial model with backward elimination statistically expressed the percent MLCT yield obtained. The optimum MLCT yield was 59.76% by using 10 wt% enzyme load, reaction temperature of 70°C, reaction time of 14 h, and substrate mole ratio of 3.5:1. Experiments to confirm the predicted results using the optimal parameters showed an MLCT yield of 56.35% (n = 2). The choice on the types of fatty acids used in MLCT optimization work greatly influenced the physical and chemical properties of MLCT oil produced. The refined MLCT oil characteristics study showed this oil is suitable to be used for cooking/frying purposes as a high-value added product.     

母乳中长链甘油三酯研究进展

母乳中甘油三酯的结构对于婴幼儿的脂肪消化吸收具有重要影响。作者所在团队前期研究结果显示,母乳脂肪天然富含中长链甘油三酯（MLCT）,是母乳中含量最丰富的一类甘油三酯,其质量约占总甘油三酯的30%,主要结构是一个中链脂肪酸和两个长链脂肪酸构成的单中链型甘油三酯（MLL）。母乳中几乎不含中链甘油三酯（MCT）。作者综述了母乳脂肪中MLCT的含量、组成、影响因素以及母乳与婴幼儿配方奶粉中甘油三酯的差异,总结了MLCT的消化特性,以及MLCT型母乳替代脂的研究进展,为新型配方奶粉脂肪的开发提供理论依据。     

Modeling and Optimization of Lipozyme RM IM-Catalyzed Esterification of Medium- and Long-Chain Triacyglycerols (MLCT) Using Response Surface Methodology

Optimization conditions of Lipozyme RM IM lipase esterification of capric and stearic acids with glycerol for the production of medium- and long-chain triacyglycerols (MLCT) fat suitable for food applications such as margarine and shortening were investigated. Response surface methodology (RSM) was applied to model and optimize the reaction conditions, namely, the reaction time (8–24 h), enzyme load (5–15 wt.%), and fatty acids/glycerol ratio (3:1–4:1) and represented by Ti, En, and Sb, respectively. Best-fitting models were successfully established for both MLCT yield (R ² = 0.9507) and residual FFA (R ² = 0.9315) established by multiple regressions with backward elimination. Optimal reaction conditions were 13.6–14.0 h for reaction time, 7.9–8.0 wt.% for enzyme load, and 3:1 for fatty acids/glycerol molar ratio. Chi-square test showed that there were no significant (P > 0.05) differences between the observed and predicted values of both models. Refined MLCT fat blend had sufficient solid fat at room temperature and made it suitable to use as a hard stock in shortening and margarine production.     

Health Benefits, Enzymatic Production, and Application of Medium- and Long-Chain Triacylglycerol (MLCT) in Food Industries: A Review

Medium- and long-chain triacylglycerol (MLCT) is a modified lipid containing medium- chain (C6-C12) and long-chain fatty acids (C14-C24) in the same triacylglycerol (TAG) molecule. It can be produced either through enzymatic (with 1,3 specific or nonspecific enzyme) or chemical methods. The specialty of this structured lipid is that it is metabolized differently compared to conventional fats and oils, which can lead to a reduction of fat accumulation in the body. Therefore, it can be used for obesity management. It also contains nutritional properties that can be used to treat metabolic problems. This review will discuss on the health benefits of MLCT, its production methods especially via enzymatic processes and its applications in food industries.     

Physicochemical and Sensory Properties of Soymilk-incorporated Bulgur

ABSTRACT Bulgur cooked in soymilk of 6% and 9% solid content had significantly ( P < 0.05) higher bulk density compared with bulgur cooked in soymilk of 3% solid content and control bulgur cooked in water. As the soymilk solid content increased, pilav bulgur yield increased whereas fine bulgur yield decreased. The SDS-extractability of bulgur proteins increases with the increase in soymilk solid content. Water absorption capacity of fine bulgur samples was affected by soymilk incorporation, and variations in oil absorption capacity were not significant ( P > 0.05). Soymilk incorporation improved color and sensory properties of pilav and fine bulgur. The results of this study suggest that soymilk can be successfully applied to bulgur production.     

大米碾白度对大米理化性质和米饭感官品质的影响

综述了碾白度对长粒，中粒米糊化特性、短粒米理化性质和米饭感官品质的影响。对米饭感官品质进行了详细说明，论述了通过仪器测定和对米饭感官品质的评价最终确定短粒米饭仪测理化性质和感官品质之间的相关系数等情况。     

Influence of Bacterial Cellulose (nata) on the Physicochemical and Sensory Properties of Frankfurter

The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated. Emulsion stability of the raw meat batter decreased with the addition of nata. For nata‐added treatments, increasing levels of nata in frankfurters resulted in decreased textural hardness and shear values. Sensory results indicated that N10 and N20 had significantly higher firmness scores than N30 and control. However, juiciness score did not differ for all treatments. Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular‐fat frankfurters. Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values. Based on the composite results, manufacture of regular‐fat frankfurter containing 10% to 20% high‐fiber nata resulted in product quality comparable to the control.     

猪牛混合脂肪的物化性质和感官特性分析

运用热性质分析、诱导氧化分析和流变分析,对牛脂肪部分替代猪脂肪后复合脂肪的物化性质及加工产品感官性质的变化进行研究。结果表明:替代添加牛脂肪后,猪牛混合脂肪的物化性质和感官性状均发生了不同程度的变化,相对于猪脂肪4.37 h的氧化反应诱导期(induction period,IP),替代10%、20%、30%猪脂肪的猪牛混合脂肪的IP值分别为4.98、5.62、6.22 h;替代10%、20%、30%猪脂肪后猪牛混合脂肪熔融时热流越来越大,晶型转换所需要的能量随着替代量的增加明显增加,总体表现为猪牛混合脂肪熔融时需要的能量变小;同时,相比于单纯的猪脂肪,猪牛混合脂肪的黏性模量和弹性模量均有一定的上升,结果也显示牛脂肪部分替代猪脂肪明显影响到乳化肠的感官品质,总体可接受性受到明显影响(P0.05)。     

江苏富士苹果的理化及感官特性分析

江苏省苹果以丰县为集中产地,富士为主栽品种,隶属于黄河故道产区,为明确其鲜食特性的地域特点,对丰县富士糖酸含量、色泽和质构等理化指标和硬性、易碎性、多汁性、酸甜度等感官属性进行了测定,并与陕西富士进行了比较。结果显示丰县苹果蔗糖和苹果酸含量偏低,分别为陕西苹果的75.88%和58.90%。江苏苹果果皮L*为64.37,a*值为11.19,b*值为32.12,a*值低而L*、b*值高于陕西苹果,说明果皮红色弱而偏黄色;果肉L*值为81.19,b*值为29.00,L*值低而b*值高于陕西苹果,说明果肉色泽偏黄。质构仪测得江苏苹果的脆度显著高于陕西苹果。感官评价结果显示江苏苹果具有硬性低、易碎、易嚼、酸度低的特点,而多汁性和甜度差异不显著。在偏爱性比较中,73.7%的评价人员偏爱陕西苹果的色泽,而对滋味和质地的偏爱程度差异不显著。研究结果将为江苏富士品质评价提供借鉴。     

Surimi-Derived Elver Substitutes: Microscopic Appearance and Physicochemical and Sensory Properties

ABSTRACT: Elver substitutes made from kamaboko-type fish gels have recently been introduced onto the market in Spain. Kamaboko is made by addition of 2.5 to 3.0% salt, and optionally other additives, to surimi (minced fish). The present study investigated the usefulness of microscopy techniques for characterizing the structure and composition of two elver substitutes of this type. Sensory and physicochemical analyses were also performed, with the aim of defining the organoleptic characteristics of these products. The results obtained indicate that microscopy techniques are useful for rapid identification of certain additives in products of this type. Furthermore, many of the between-brand differences in microscopic appearance showed clear correspondence with differences in physicochemical and sensory properties.     

中长链甘油三酯对大鼠肝脏脂质代谢的调节作用

为研究中长链甘油三酯(MLCT)在短期内对大鼠肝脏脂质组成及脂代谢的影响,选取雄性SD大鼠随机分为空白对照组、中长链甘油三酯组(MLCT组)、中链甘油三酯组(MCT组)、高油酸菜籽油组(HORO组),每日以1g/100g体重的剂量灌胃相应脂质2周。结果显示:与HORO组相比,MLCT显著降低大鼠血浆中甘油三酯(TG)和总胆固醇(TC)的含量,且降低低密度脂蛋白胆固醇(LDL-C)的含量;MLCT显著抑制大鼠肝脏质量的增加,降低肝脏中TC、TG含量,使载脂蛋白A1/载脂蛋白B比值显著性升高。各组大鼠肝脏中甘油三酯、磷脂(PL)的脂肪酸组成变化显著,说明摄入不同脂质可导致肝脏脂肪酸组成的改变。MCT组大鼠肝脏中激素敏感性脂肪酶(HSL)、环磷酸腺苷(cAMP)和蛋白激酶A(PKA)水平最高,脂肪甘油三酯脂酶(ATGL)水平最低。相反,HORO组的HSL、cAMP和PKA水平最低,而ATGL水平最高。MLCT组上述脂质代谢相关酶水平均介于MCT、HORO组之间,且均高于对照组。本研究表明,MLCT在2周内可有效改善大鼠体内脂质代谢,提高肝脏中脂质代谢的效率,从而降低脂代谢以及相关疾病的发生风险。     

Influence of Homofermentative Lactobacilli on Physicochemical and Sensory Properties of Cheddar Cheese

Cheddar cheeses were produced under pilot plant conditions using a commercial Streptococcus culture amended with one of 10 homofermentative Lactobacillus strains. During the ripening period, pH, acidity, salt, moisture, fat, texture, fissure formation, gas development and sensory status were evaluated. Lactobacillus treated cheese did not differ much from the control in pH and acidity but acidity increased substantially after draining and cheddaring. Lactobacillus numbers increased at all stages as compared with the uninoculated control. High quality Cheddar cheese was produced by L. casei-subsp-casei (119-10/62) and L. casei-subsp-pseudoplantarum (137-10/62) from 7 to 12 vats aged for 2 months at 15°C and for a further 10 months at 7°C or 15°C. Fissure formation was observed in cheese made with L. casei-subsp-rhamnosus, one of the four cultures of L. casei-subsp-casei (LH13) and two of the three strains of L. casei-subsp-pseudoplantarum (83-4-12/62 and L3E). Certain Lactobacillus strains produced cheese with slight flavor defects. Other strains, in particular L. casei-subsp-rhamnosus, contributed to high acidity (72 - 0.89° domic) and low pH (5.2) at salting.     

Physical Properties, Sensory Attributes, and Consumer Preference of Pear Fruit Leather

ABSTRACT: Pear fruit leathers were prepared by drying a mixture of pear juice concentrate, pectin, corn syrup, and water at 70°C for 8 h. The effects of ingredients on texture, color, microbial growth, moisture content (MC), water activity (a_w), glass transition temperature (T_g), and sensory attributes were investigated. Pectin was the most significant independent variable that affected the properties of interest. This was followed by initial moisture content and corn syrup. Glass transition temperature (T_g) was not useful for predicting microbiological attributes but had strong correlations with instrumental and sensory hardness and chewiness. The results from partial least squares analysis (PLS) indicate instrumental hardness, chewiness, and cohesiveness could be used to predict their corresponding sensory attributes. The consumer liking of pear fruit leather could be increased by raising their fruit aroma, sweetness, tartness, and shininess.     

油炸过程中淡水小龙虾理化性质与品质变化

研究分析油炸工艺（油炸温度（140、160、180、200 ℃）和油炸时间（15、30、45、60 s））对淡水小龙虾（虾壳、虾肉、虾黄）理化性质及品质的影响。测定淡水小龙虾中心温度、油炸损失率、色泽、质构特性、挥发性盐基氮（total volatile basic nitrogen,TVB-N）含量、硫代巴比妥酸反应物（thiobarbituric acid reactive substances,TBARs）值以及菌落总数（total plate count,TPC）的变化。结果表明：油炸温度对虾肉中心温度影响不显著,而油炸时间对虾肉中心温度影响显著（P＜0.05）;油炸温度180 ℃、油炸45 s时,虾肉中心温度可达到肌肉熟制标准温度;随着油炸温度升高及油炸时间延长,小龙虾油炸损失率显著上升（P＜0.05）;虾壳、虾肉、虾黄亮度值、红度值均呈上升趋势,而黄度值呈下降趋势;虾肉硬度、回复性、弹性、黏性、咀嚼性均显著增加（P＜0.05）;油炸过程中,虾肉TVB-N含量显著增加（P＜0.05）,但均低于10 mg/100 g,表明虾肉油炸后保持较好的新鲜度;虾肉TBARs值随油炸温度升高、油炸时间延长而增加,其中油炸时间对TBARs值影响较明显;新鲜虾肉初始带菌量非常高,油炸处理后虾肉TPC显著降低（P＜0.05）,油炸时间对虾肉TPC影响不明显。     

UHT乳贮藏期间理化特性和感官品质的变化

为探讨超高温瞬时杀菌(ultra-high temperature instantaneous sterilization,UHT)乳的营养价值随贮藏时间的变化,对不同贮藏期UHT乳的理化特性和感官品质进行监测。在6个月的贮藏期内,UHT乳中脂肪、蛋白质、乳糖以及固形物的总量并无明显变化,维生素C和泛酸的含量均损失了约25%,游离氨基酸和钙离子分别增加了5.35%和6.73%。不同贮藏期UHT乳的黏度变化不显著(P>0.05),但粒径大小强烈依赖于贮藏时间,乳脂肪球和酪蛋白胶束的尺寸在贮藏初期分别增加了约38 nm和14 nm,然后分别稳定在约173 nm和612 nm。贮藏1个月的UHT乳样品在风味和口感方面整体表现较好,贮藏6个月后,UHT乳的感官品质大幅下降,出现了许多不良风味。     

Physicochemical and Sensory Properties of Corn Tortillas Made from Nixtamalized Corn Flour Fortified with Spent Soymilk Residue (okara)

ABSTRACT: The chemical study of okara composition and a sensory evaluation study were conducted to determine the effect of okara supplementation on certain physical, chemical and sensory properties of corn tortilla. Dried okara was added at the level of 5,10,15,20, and 25% to nixtamalized corn flour. Due to flavor, enriched tortillas at levels higher than 10% of okara were not accepted. At 10% of okara supplement, lysine and tryptophan content in tortilla were increased from 56 and 70% of FAO profile to 93 and 92% respectively.     

微生物发酵对青稞挂面理化及感官特性的影响

为探究不同菌种发酵对青稞挂面理化及感官特性的影响,采用植物乳杆菌、酵母菌及复合菌种(m(酵母菌)︰m(乳酸菌)=1︰1)分别发酵青稞制成青稞粉,并与小麦粉混合压制成挂面,以挂面的理化及感官特性为指标,确定适宜发酵方式及工艺。与植物乳杆菌发酵和复合菌种发酵相比,酵母发酵青稞面条断条率低,吸水率高,蒸煮时间长,感官品质好,说明酵母发酵能更好地改善青稞挂面理化及感官特性。通过单因素试验及正交试验优化后的酵母发酵青稞的工艺参数为发酵时间6 h、发酵温度32℃、接种量0.8%。