Variations in zooplankton community structure and water quality conditions in three habitat types in northern Lake Victoria

Lake Victoria is vulnerable to increasing eutrophication, which has become manifested in ecological changes not yet fully understood. From October 2009 to January 2010, the influence of water quality on zooplankton community structure in three habitats in northern Lake Victoria, including sewage lagoons at the lake shores, Napoleon Gulf (NG) and the interface between the lake and the Nile River (also known as Source of the Nile River), was examined. Selected physico‐chemical parameters (dissolved oxygen concentration; water temperature; electrical conductivity; water depth) were measured in‐situ, while water samples were collected for chlorophyll‐a determinations. Zooplankton was sampled with conical plankton net (mesh size 60 μm; 0.25‐m mouth diameter). The NG and Source of the River Nile (SN) sampling sites exhibited significantly higher species richness, relative to the Sewage Lagoons (SL) site (F_2,69 = 68.533; P < 0.05). Higher mean densities and dry biomass of zooplankton was generally a characteristic of the SL site (8715 ± 3241 ind L^?1; 1862 ± 451 μg L^?1), compared to the NG (119 ± 24 ind L^?1; 53 ± 8 μg L^?1) and SN sites (151 ± 26 ind L^?1; 58 ± 9 μg L^?1). Copepoda constituted a high numerical composition of the zooplankton at the NG and SN sites (>90% for both sites), while Rotifera dominated the zooplankton community at the SL site (97%). The mean values (±SE) of soluble reactive phosphorus (4060.7 ± 776.6 μg L^?1) and nitrate–nitrogen (2121.7 ± 355.5 μg L^?1) were much higher for the SL site, compared with the SN (8.2 ± 1.1 μg L^?1; 28.6 ± 5.3 μg L^?1, respectively), and NG site (7.8 ± 0.8 μg L^?1; 32.7 ± 5.4 μg L^?1, respectively). This study indicated the nutrient‐rich conditions observed at the SL site suppress the zooplankton species diversity, but favour species‐specific abundance and biomass. These study results indicate the zooplankton community structure can be used as a biological indicator of water quality in the Lake Victoria region.     

Effects of environmental factors on seasonal and spatial changes in surface zooplankton in Golden Horn Estuary (Istanbul,Turkey)

The abundance and spatial distribution of zooplankton at monthly intervals were studied in polluted Golden Horn Estuary (Istanbul, Turkey), from July 2006 to December 2007. Selected water quality parameters also were measured. The zooplankton community was characterized by the presence of freshwater, estuarine and marine species, with a total of 59 taxa. Rotifers were the most important fraction of the total zooplankton, comprising 36.52% of all the samples. The zooplankton exhibited seasonal patterns, with the highest abundance occurring in the summer (979 ind. L^?1) and the lowest in the spring (856 ind. L^?1). Rotifera was the most abundant group in the upper and middle estuary, whereas Copepoda usually reaches the maximum densities in the higher salinity waters in the lower estuary. The study results indicated that both the sampling time (seasons) and sampling sites jointly had significant effects on the zooplankton densities (manova P < 0.05), and horizontal patterns were related to electrical conductivity, water temperature, dissolved oxygen, suspended particulate matter, pH and chlorophyll a measured in the estuary.     

Longitudinal Variability in Hydrochemistry and Zooplankton Community of a Large River: A Lagrangian‐Based Approach

The variability in water quality and zooplankton community structure during downstream transport was investigated in the Po river (Italy) using for the first time a Lagrangian sampling approach. Two surveys were conducted, one in spring under relatively high discharge levels, and one at low flows in summer. Twelve stations along a 332‐km stretch of the river's lowland reach and four major tributaries were sampled. A hydrodynamic modelling system was used to determine water transport time along the river, with a satisfying fit between simulated and observed discharge values. No clear downstream trend in phosphorus and nitrogen concentrations was found. Conversely, a marked longitudinal decrease in dissolved silica supports the hypothesis of increasing downstream silica limitation during the phytoplankton growing season. In spring, at low residence time, no apparent plankton growth was observed during downstream transport. In summer, higher temperatures and lower turbulence and turbidity associated with longer residence time stimulated algal growth and in‐stream reproduction of fast‐growing rotifer taxa, with the gradual downstream development of a truly potamal assemblage and the increase of the ratio of euplanktonic to littoral/epibenthic rotifer taxa. Crustacean zooplankton density was generally low. The importance of biotic interactions within the zooplankton in driving community abundance and composition appeared to increase in the downstream direction, paralleled by a decrease in the influence of physical forcing. Tributary influence was especially evident where severe anthropogenic alterations of river hydrology and trophic status resulted in enhanced plankton growth, ultimately affecting zooplankton structure in the main river. Copyright © 2016 John Wiley & Sons, Ltd.     

Spatial variations in nutrients and other physicochemical variables in the topographically closed Lake Baringo freshwater basin (Kenya)

Spatial physicochemical parameters were determined from 39 sampling sites distributed throughout Lake Baringo during December 2010. Mean values of temperature, dissolved oxygen concentration and electrical conductivity decreased successively with depth, while the pH remained constant. Only the turbidity values increased marginally with depth. Of the surface water parameters, mean (range) values of dissolved oxygen (DO), pH, electrical conductivity, water transparency and turbidity were 6.9 (4.5–8.4) mg L^?1, 8.3 (7.8–8.5), 573 (556–601)μS cm^?1, 33 (28–37) cm and 43.3 (32.7–54.6) NTU, respectively. Mean and range values of total nitrogen (TN), nitrate‐nitrogen(NO₃‐N), ammonia nitrogen (NH₄‐N), total phosphorus (TP) and soluble reactive phosphorus (SRP) were 788.4 (278–4486) μg L^?1, 4.5 (2.4–10.0) μg L^?1, 42.6 (33.8–56.3) μg L^?1, 102.9 (20.3–585.3) μg L^?1 and 23.5 (15.2–30.5) μg L^?1, respectively. Dissolved silica concentrations ranged from 19.7 to 32.7 mg L^?1, with a mean value of 24.7 mg L^?1. The chlorophyll‐a concentrations were quite low, ranging from 1.4 to 4.9 μg L^?1, with a mean value of 4.2 μg L^?1. In contrast to previous reported values, a key finding in the present study is a relatively high water transparency, indicating a relatively clear water column, due possibly to the fact that the sampling was conducted during the dry period. The nutrient levels remained low, and the chlorophyll‐a concentration also was an almost all time low value. A TP value of 20 μg L^?1 and higher confirms strongly eutrophic conditions prevailing in the lake, with an extremely low potential for fish production and low species diversity, consistent with other studies. The results of the present study, therefore, reinforce the database for future management and monitoring plans for the Lake Baringo ecosystem, which lies adjacent to known geothermally active zones and a saline Lake Bogoria.     

Zooplankton as an indicator of trophic conditions in two large reservoirs in Brazil

The physical and chemical variables of the water, and the composition and structure of the zooplankton communities, in Três Marias and Furnas Reservoirs in Minas Gerais, Brazil, were compared to characterize these environments in relation to their trophic state. Higher values of electrical conductivity and chlorophyll‐a, total solids, suspended organic matter and total nitrogen concentrations were recorded in Três Marias Reservoir. Higher water transparency and nitrite and nitrate concentrations were observed in Furnas (P < 0.000). Higher zooplankton densities were always obtained in Três Marias Reservoir and, during the rainy period (P < 0.000), with mean values in the dry and rainy periods of 23 721 and 90 872 org m^?3, respectively, in Três Marias Reservoir and 9022 and 40 434 org m^?3, respectively, in Furnas Reservoir. Copepoda was the dominant group in both reservoirs, mainly the younger stages (nauplii and copepodids). Based on the absolute and relative values, the contribution of rotifers was higher in Três Marias Reservoir than in Furnas Reservoir. Although the Trophic State Index, based on water transparency and chlorophyll‐a and total phosphorus concentrations, indicated an oligotrophic state for both reservoirs, the higher densities of the zooplankton community in Três Marias Reservoir, as well as the predominance of cyclopoids and smaller‐sized species such as bosminids, characterized this environment as mesotrophic. Larger‐sized species such as calanoids, daphniids and sidids in Furnas Reservoir characterized the better water quality of this reservoir.     

Influence of Hydrological Connectivity on Plankton Communities in Natural and Reconstructed Side‐Arms of a Large New Zealand River

We sampled natural and reconstructed side‐arms during different stages of hydrological connectivity with a large floodplain river in northern New Zealand, to determine whether re‐establishment of connectivity would be an effective strategy for restoring plankton communities in former side‐arms. Connectivity between side‐arms and the river was moderated by water level and influenced flow rates and closure of inlets and outlets. Physicochemical conditions were more strongly related to the connectivity phase than to habitat type (river, natural or reconstructed side‐arm), except during low connectivity when natural side‐arms in particular were characterised by higher ammonium (NH₄‐N) and total phosphorus (P) concentrations, as well as specific conductivity. Dissolved reactive phosphorus (PO₄‐P), water temperature, conductivity and dissolved oxygen were identified as explanatory variables of phytoplankton and zooplankton community composition, which along with total nitrogen (phytoplankton) or total suspended solids (zooplankton) explained 44–52% of variation. Phytoplankton community composition and the abundance of several dominant or discriminatory taxa were affected by connectivity but not habitat type, whereas habitat and connectivity both had significant effects on zooplankton communities and abundances of the cladoceran Bosmina meridionalis. Significant interactions between connectivity and paired habitat types occurred for abundances of the diatom Asterionella, the cryptophyte Cryptomonas, the rotifer Synchaeta oblonga and cyclopoid copepods, reflecting differential responses to connectivity among habitats by these taxa. Overall, these results underscore the importance of hydrological connectivity between side‐arms and rivers in moderating plankton community composition, and highlight unpredictable trajectories of community development and alternative transient states that can occur soon after side‐arm reconnection. Copyright © 2016 John Wiley & Sons, Ltd.     

Eutrophication of lakes in urbanized areas: The case of Yaounde Municipal Lake in Cameroon, Central Africa

A 1 year qualitative and quantitative evaluation of phytoplankton and chlorophyll‐a, as well as some physicochemical parameters, was recorded in a shallow tropical lake in Cameroon: the Yaounde Municipal Lake. Physicochemical measurements also were regularly made in its main tributary (Mingoa Stream). These analyses aimed to assess the lake's trophic status and to propose measures for controlling its degradation process. The Secchi disk transparency was low and rarely exceeded 100 cm. Conductivity was higher near the lake bottom. The oxygen deficiency, and sometimes anoxia, recorded from a 2.5 m depth leads to high quantities of ammonium‐nitrogen. The total phosphorus concentrations varied from 80–2290 µg P L^?1 and the total Kjeldhal nitrogen concentrations fluctuated between 3 and 15 mg  L^?1. Upstream to the lake, in the Mingoa Stream, total phosphorus concentrations ranged from 0.6–3.8 mg P L^?1 and total Kjeldhal nitrogen concentrations ranged from 10–22 mg  L^?1. There are up to 102 phytoplankton‐specific taxa, with Euglenophyta and Chlorophyta particularly more diversified. The phytoplankton biomass and chlorophyll‐a concentrations reached 225 µg mL^?1 and 566 mg m^?3, respectively. The analyses pointed out the allogenic nature of the functioning of this ecosystem as a result of bad waste management in the surrounding landscape. Urgent actions need to be undertaken in order to rehabilitate this lake, which rapidly shifted to a hypertrophic status.     

Recruitment of Heliozoa, rhizopods and rotifers from the sediments of an extremely acidic lake during spring and early summer

The goal of this study was to investigate the recruitment of zooplankton from the littoral sediment of Lake 111, an acidic lake in north‐east Germany, in April (spring) and June (early summer), and its role in coupling the benthos and the pelagic. Maximum heliozoan and rhizopod recruitment occurred in early summer from sediment cores incubated at ambient water temperatures (20°C). Conversely, recruitment of the rotifer Cephalodella sp. was highest in spring at ambient spring temperatures of 12°C. A combination of passive and active recruitment processes is likely responsible. The seasonal abiotic and biotic sediment characteristics were relatively constant and therefore not likely responsible for the observed temporal recruitment pattern. The sediment water and carbon content ranged from 20 to 50% (mean = 29 ± 6% standard deviation) and 2–12% (mean = 5 ± 2% standard deviation), respectively. Similarly, there was little variation in the chlorophyll‐a (mean = 0.2 ± 0.2 µg Chl‐a g^?1 dry weight ≡ 6.1 ± 3.9 mg Chl‐a m^?2). The in situ sediment bacterial density (0.82 × 10⁹ ± 0.26 × 10⁹ g^?1 dry weight ≡ 1.01 × 10⁹ ± 0.34 × 10⁹ cells cm^?3) was high. In contrast, the abundance of zoobenthos and their resting stages was low (< 25 individuals cm^?3, and mean of 90 ± 75 cysts cm^?3, respectively), with no temporal pattern being observed. Temperature was the only abiotic factor influencing recruitment. This study suggests that, even in relatively young, chemically extreme lakes, the benthos can play an important role in whole lake microbial processes and zooplankton community composition. Such benthic repositories of resting stages potentially provide protection against adverse environmental changes.     

A Seasonal Study Reveals the Occurrence of Exotic Rotifers,the River Antigua,Veracruz, Close to the Gulf of Mexico

Zooplankton studies in Mexican rivers are few despite the fact that Mexico has >200 rivers. We present data on the seasonal diversity of rotifers during 2013–2014 from the river La Antigua, near Veracruz. We collected samples from 15 stations along a horizontal gradient of ~5 km, from the upper reaches to about 2 km from the sea. The physico‐chemical variables analysed were temperature, pH, dissolved oxygen, conductivity, nitrates, phosphates, Secchi depth and salinity. From each site, 80 L of water was filtered with a 50‐μm plankton net. Rotifers were identified and quantified using a Sedgewick Rafter cell. The river is shallow (maximum depth 2.5 m during the rainy season). Our observations indicated that the phytoplankton community was dominated by diatoms; the chlorophyll a concentrations ranged from 0.55 to 26.1 μg L^?1 over the study period. We found >125 rotifer species belonging to 21 families, mostly from the Lecanidae, Brachionidae, Notommatidae and Lepadellidae. The density of rotifers was low, <40 ind. L^?1, but species diversity (Shannon–Wiener) ranged, except for a onetime minimum of 0.25, from 2.0 to 4.0 for most part of the year. We also encountered Notholca cf. liepetterseni , an endemic species to Europe and Lecane yatseni , endemic to Asia. Other new records included Lecane rhytida and Ptygura melicerta . The importance of long‐term studies in rivers is emphasized. Copyright © 2017 John Wiley & Sons, Ltd.     

Benefits to 0+ fishes of connecting man‐made waterbodies to the lower River Trent,England

Floodplain waterbodies are reputed to enhance recruitment of riverine fish populations via provision of spawning and nursery habitat, refuge from floods, and increased availability of planktonic food resources compared with main river channels. Notwithstanding, there have been few parallel studies of fishes and their food resources at both main river and floodplain sites. Thus, this study investigated the 0+ fishes, zooplankton and phytoplankton (chlorophyll a) at four main river and four (man‐made) floodplain sites on the lower River Trent, England, between May 1999 and October 2004 inclusive. All sites shared the same key fish species, and there were no consistent differences in the densities, growth or condition of 0+ fishes from main river and floodplain sites. Similarly, all sites shared the same key zooplankton taxa. However, zooplankton densities, notably of large‐bodied cladocerans, and chlorophyll a concentrations, were significantly higher at floodplain sites than at main river sites. Thus, connection of man‐made waterbodies has the potential to enhance recruitment of riverine fish populations via provision of important spawning and nursery habitat, and superior feeding opportunities for 0+ fishes compared with main river channels. Copyright © 2007 John Wiley & Sons, Ltd.     

Phytoplankton composition and biomass in tropical soda Lake Shala: seasonal changes in response to environmental drivers

Although soda lakes are valuable, sensitive aquatic resources where phytoplankton play a decisive role for the entire ecological functions, they are among the least‐studied ecosystems. Seasonal variations in phytoplankton composition, abundance and biomass in relation to some environmental parameters of the little known, deep, large, volcanic and saline–alkaline Lake Shala were investigated over an annual cycle. The lake phytoplankton community consisted of relatively diverse taxa (23) belonging to Bacillariophyceae, Cryptophyta, Cyanoprokaryota and Dinophyta. Bacillariophyceae and Cryptophyta were the dominant groups throughout the annual cycle, accounting for about 57% and 22% of the total number of species, and 28% and 69% of the total abundance of the phytoplankton community, respectively. Cryptomonas spp. were most abundant throughout nearly all months, contributing about 59%–95% of total phytoplankton abundance, followed by Thalassiosira sp. (1%–35%). The chlorophyll‐a concentration, as a proxy for algal biomass, was generally low (mean 17 μg L^?1), exhibiting only small seasonal variation. The strong, inverse relation of chlorophyll‐a with water transparency (r = ?0.69; n = 11) and the persistent dominance of species adapted to low‐light conditions and mixing suggest the overriding importance of these factors in controlling the lake's phytoplankton. The results of the present study generally suggest the phytoplankton composition and biomass in Lake Shala exhibited muted seasonal changes, despite the environmental perturbations, probably because of the lake's high buffering capacity against allochthonous impacts because of its voluminous nature.     

EVALUATION OF IN SITU FLUOROMETRY TO DETERMINE CYANOBACTERIAL ABUNDANCE IN THE MURRAY AND LOWER DARLING RIVERS,AUSTRALIA

A Yellow Springs Instruments water quality sonde fitted with fluorometric probes for phycocyanin and chlorophyll a was trialled at sites along the Murray and Lower Darling Rivers, Australia, during 2008–2009. The project examined whether the in situ quantification of phycocyanin by fluorometry could be used to determine the abundance of cyanobacteria present. Abundance was measured in the laboratory as biovolume from samples collected at the same time as the phycocyanin measurements. The study found a strong positive relationship between the two measurements. However, it was found that the use of in situ phycocyanin fluorometry was not effective in turbid water higher than 50 Nephelometric turbidity units (NTU) as this produced false‐positive readings for phycocyanin. There was a considerable amount of variance within the data, much probably caused by error in estimating cyanobacterial biovolumes. Some variability may also be attributed to error involved in the in situ measurement of phycocyanin, especially when cyanobacterial abundance was close to the lower limit of quantification of the instrument. This is likely to be greatest when total cyanobacterial biovolume is less than 0.4 mm³ L^?1. The relationship between phycocyanin measured in situ by fluorometry and total cyanobacterial biovolume varied spatially between sites along the Murray River, probably caused by differences in cyanobacterial abundance and in the species composition of the cyanobacterial communities in different sections of the river. No relationship was found between total cyanobacterial biovolume and chlorophyll a measured by in situ fluorometry. However, in situ fluorometry has the potential to be a useful rapid assessment tool for cyanobacterial bloom management. Copyright © 2012 John Wiley & Sons, Ltd.     

Impacts of Microcystis on algal biodiversity and use of new technology to remove Microcystis and dissolved nutrients

Cyanobacteria often dominate eutrophic lakes, outcompeting green algae that are required by fish and zooplankton. This study was undertaken to ascertain the impact of the cyanobacterium, Microcystis, on algal biodiversity. Under laboratory conditions, we found that the presence of Microcystis decreased phyla richness by 58%, phyla evenness by 47%, genera richness by 66% and genera evenness by 51%. Analysis by mixed anova s demonstrated a significant interaction between treatment and time and confirmed a significant reduction in richness and evenness of phyla and genera. We also conducted a phosphate restriction assay on the algae in Mason Lake (Irvine, CA, USA) and found that the threshold needed for algal growth there was 0.02 mg L^?1 PO₄. A pilot study was then conducted to test the effectiveness of the Blue Pro? water treatment facility in removal of this colonial organism from Mason Lake, in addition to removal of dissolved nutrients required for its growth. We measured a 97% reduction in Microcystis cells, a 72% reduction in chlorophyll‐a, and a 96% reduction in phosphate after just one 10 min cycle through the unit. Our study demonstrated that removal of Microcystis colonies may allow green algae to increase in numbers. This may improve algal biodiversity, which will benefit zooplankton and fishes.     

Long‐term irreversible changes in a lake ecosystem affected by the Indian Ocean Tsunami

This study focused on ecosystem responses to the environmental perturbations caused by the 2004 Indian Ocean Tsunami in a small lake that was a freshwater body in 1996, prior to the tsunami. The physicochemical and biological characteristics of Kokilamedu Lake (KKM) revealed drastic changes, compared with pretsunami conditions. Monthly average observations on water quality indicated the electrical conductivity of the water increased steeply to 17.41 mS cm^?1 in 2009, from the lowest pretsunami value of 1.83 mS cm^?1 (range of 1.83–5.25 mS cm^?1). Simultaneously, the nitrate + nitrite (NO₃ + NO₂) values increased significantly from 0.49 μmol L^?1 in 1996 to 74.47 μmol L^?1 in 2006. Silicate (SiO₄‐Si) exhibited a dramatic increase in concentration, from an average of 64.87 μmol L^?1 in the pretsunami period to 309.71 μmol L^?1 the post‐tsunami period (2009–2010). Inorganic phosphate had increased to a maximum of 9.59 μmol L^?1 from a pretsunami maximum of 1.09 μmol L^?1. The chlorophyll‐α concentrations did not respond to the increased nutrient stoichiometry of the lake. There was a decreased chlorophyll‐α concentration under post‐tsunami conditions. The recent infilling of the lake with sediment during the tsunami, associated with wind‐driven resuspension reduced the light penetration. There was a significant improvement in dissolved oxygen concentrations (2006–2010 average of 8.27 mg L^?1) in the lake, however, compared with the pretsunami values (1994–1995 average of 5.94 mg L^?1). The algal component is now dominated by blue‐green algae, while green algae had dominated in the pretsunami period. Pre‐ and post‐tsunami observations from a control site did not exhibit such dramatic shifts from the 1995 and 1996 conditions, whereas a shift was apparent in the case of KKM. Certain marine fishes have adapted to this altered ecosystem. These marine species encountered (Elops machnata, Cociella punctata, Sphyraena jello, Platycephalus indicus, Glossogobius giuris) might have been recruited during the intrusion of the tsunami waves.     

Longitudinal Plankton Dynamics in the Rivers Rhine and Elbe

We compared the longitudinal plankton development in the two large rivers Rhine and Elbe by means of four Lagrangian sampling campaigns performed within the time span 2009–2011. The campaigns revealed low chlorophyll concentrations in the Rhine along a long river stretch (Rhine‐km 170 to 854) with maximum values below 5 µg L^?1 in 2010. In contrast, the Elbe (Elbe‐km 4 to 582) showed high and longitudinally increasing chlorophyll concentrations with maximal values of 174 µg L^?1 in 2009 and 123 µg L^?1 in 2011. Additional samples of the benthic bivalves along the river stretches revealed high densities of the filter feeders in the Rhine that could potentially explain losses of plankton production. Their densities in the Elbe were significantly lower, making important losses to benthic filter feeders unlikely. However, strong phytoplankton growth was observed during the sampling campaign in 2011 in the Rhine coinciding with a low discharge event. This resulted in an exceptionally high chlorophyll value of up to 244 µg L^?1 in the lower river sections, a value that was not reached in the last two decades of continuous water quality monitoring in the Rhine. Even though we cannot fully explain this phenomenon, it shows that phytoplankton has a high growth potential in the Rhine but is usually controlled by other mechanisms. Tributaries represented an additional and important source of plankton biomass and suspended substances in the Rhine, whereas they primarily diluted the plankton concentrations in the Elbe. Copyright © 2015 John Wiley & Sons, Ltd.     

Reconstruction of pristine morphology,flow, nutrient conditions and submerged vegetation of lowland river spree (Germany) from palaeomeanders

The European Water Framework Directive requires the definition of reference conditions for each type of surface waters as a base to establish a classification system in which deviations from this high quality status must be determined. In order to reconstruct pristine conditions in the lower river Spree we investigated palaeomeanders using palaeohydrological and palaeolimnological methods. The settlement history of this region suggests low anthropogenic impact for all periods before ～700 cal AD. Three palaeomeanders representing the conditions of the late Sub‐boreal/early Sub‐atlantic were investigated. River width and depth at bankfull stage were reconstructed using cross‐sections of the meanders. Based on these data and experiments on the recent river, a parametric model was developed to calculate the bankfull palaeodischarge. Reconstructions show narrower and shallower channels for the undisturbed lower Spree (～20 m mean width and 0.8 m mean depth at bankfull stage) as compared to recent conditions (35 m and 1.6 m, respectively). Flow velocities and discharge at bankfull stage have been smaller in reconstructed sub‐fossil channels (0.5 m s^?1 and 8 m³ s^?1 in the pristine lower Spree as compared to 0.9 m s^?1 and 52 m³ s^?1 in the recent lower Spree) and flow variability was higher. The increase in bankfull discharge was mainly attributed to deforestation and drainage of the catchment as well as channelization, bank protection and river regulation measures. The organic silt at the base of the sediment cores contained well‐preserved fossil diatom assemblages. Diatom‐inferred total phosphorus (DI‐TP) concentrations of 59–73 µg L^?1 (median 62 µg L^?1) indicate eutrophic to hypertrophic conditions and suggest naturally slightly lower nutrient levels than today. These past nutrient conditions, morphology and large numbers of macrofossil remains indicate optimum growth conditions for submerged macrophytes growth. The combination of palaeohydrological and palaeolimnological parameters proved to be a useful approach for the determination of pristine conditions in a lowland river. Copyright © 2008 John Wiley & Sons, Ltd.     

Impact of an urban centre on the nitrogen cycle processes of epilithic biofilms during a summer low‐water period *

Nitrogen transformations in epilithic biofilms of a large gravel bed river, the Garonne, France, has been studied upstream (one site) and downstream (four sites) of a large urban centre (Toulouse, 740 000 inhabitants). High biomass, up to 49 g AFDM m^?2 (ashes free dry matter) and 300 mg chlorophyll a m^?2 (Chl. a), were recorded at 6 and 12 km downstream from the main wastewater treatment plant outlet. The lowest records upstream and larger downstream (less than 16 g AFDM m^?2 or 120 mg Chl. a m^?2) could be explained by recent water fall (early summer low‐water period). Measurements of nitrogen exchange at the biofilm–overlying water interface were performed in incubation chambers under light and dark conditions. The addition of acetylene at the mid‐incubation time allowed evaluation of both nitrification (variation in NH₄⁺ flux after the ammonium monooxygenase inhibition) and denitrification (N₂O accumulation related to the inhibition of N₂O reduction). Denitrification (D_w) and nitrification rates were maximum at sites close to the city discharges in dark conditions (up to 9.1 and 5.6 mg N m^?2 h^?1, respectively). Unexpected denitrification activities in light conditions (up to 1.4 mg N m^?2 h^?1) at these sites provided evidence for enhanced nitrogen self‐purification downstream. As confirmed by most probable number (MPN) counts, high nitrification rates in biofilm close downstream were related to enhanced (more than almost 3 log) nitrifying bacteria densities (up to 7.6×10⁹ MPN m^?2). Downstream of an urban centre, nitrogen transformations in the biofilm appeared to be influenced by the occurrence of an adapted microflora which is inoculated or stimulated by anthropic pollution. Copyright © 2002 John Wiley & Sons, Ltd.     

Importance of biotic interactions in large rivers: an experiment with planktivorous fish,dreissenid mussels and zooplankton in the St. Lawrence River

Physical conditions are usually considered pre‐eminent in controlling river plankton, but biotic interactions may be important in slackwater areas. To begin testing this general hypothesis, we conducted a 12‐day, predator–prey experiment in 3500 litre mesh enclosures in a slackwater area of the St. Lawrence River using planktivorous, juvenile yellow perch (Perca flavescens) and Dreissena mussels. Results generally supported our hypotheses that: (1) perch would directly suppress large zooplankton via predation but benefit microzooplankton through indirect interactions; and (2) dreissenids would directly depress rotifer densities via predation and have indirect negative effects on macrozooplankton. Based on gut contents of experimental fish, cladocera were the principal prey of smaller yellow perch (c. 46–50 mm), followed by copepods, ostracods, and rotifers. Larger juvenile perch (c. 67–73 mm) fed almost exclusively on copepods and ostracods. Densities of calanoid copepodids, nauplii, and some cyclopoid copepods (Diacyclops thomasi) were significantly depressed by perch, and adult Eurytemora affinis (99% of adult calanoids) essentially disappeared from fish enclosures. Despite being a favourite prey item of small perch, densities of the small cladoceran Bosmina (Sinobosmina) spp. were significantly higher when fish were present (150% greater than control densities on Day 12). Densities of the very abundant rotifer Polyarthra were >300% greater in fish enclosures than controls by Day 12, and the rotifers Synchaeta and predaceous Ploesoma were significantly more abundant in the presence of perch. Increases in rotifers and cladocera suggest indirect, positive effects of fish related to significantly higher phytoplankton biomass or decreased densities of predaceous copepods. Densities of eight of ten zooplankton groups examined declined significantly when mussels were present, and calanoid copepodids also declined but not significantly. Chlorophyll‐a concentrations were slightly lower in mussel enclosures. This evidence suggests biotic interactions play important roles among potamoplankton in slackwater habitats, but river‐wide implications of these findings require further study. Copyright © 2003 John Wiley & Sons, Ltd.     

EFFECTS OF ARTIFICIAL FLOODING ON WATER QUALITY OF A FLOODPLAIN BACKWATER

Seasonal flooding of riverine backwaters is important in maintaining diverse aquatic habitats, but anthropogenic impacts have reduced the frequency and duration of such flooding. This study, conducted in a 2.5‐km‐long shallow floodplain severed meander backwater adjacent to the Coldwater River in Tunica County, Mississippi, USA, compared water quality during a late summer 30‐day artificial flooding period with 28‐day pre‐flood and 26‐day post‐flood periods. Flooding was simulated by pumping 0.22 to 0.35 m³ s^?1 from the river into the upstream portion of the backwater. In situ parameters (temperature, pH, dissolved oxygen, conductivity and fluorescent chlorophyll) were measured every 30 min at one site within the backwater. Solids (dissolved and suspended) and nutrients (phosphorus and nitrogen) were measured at three sites in the backwater and in the river every 3 to 5 days. Decreases in the amplitude of temperature, dissolved oxygen and pH diel cycles within the backwater were observed during flooding. Changes in patterns of solids and nutrients were also associated with flooding. Complex patterns in phosphorus and nitrogen emerged as a result of utilization by autotrophs (measured as chlorophyll) and seasonal changes. Artificial flooding in a shallow floodplain water body stabilized and improved water quality for aquatic biota and is a viable method for habitat rehabilitation in these systems. Copyright © 2011 John Wiley & Sons, Ltd.