共查询到20条相似文献，搜索用时 15 毫秒
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Ayako Yamada Fanny Barbaud Lucia Cinque Li Wang Qian Zeng Yong Chen Damien Baigl 《Small (Weinheim an der Bergstrasse, Germany)》2010,6(19):2169-2175
A simple and robust method to compartmentalize aqueous solutions into an array of independent microchambers is presented. The array of microchambers fabricated in poly(dimethylsiloxane) are filled with the sample solution through a microfluidic channel and then sealed with oil to isolate the microchambers from each other. A water reservoir close to the microchambers allows the maintainance and incubation of sub‐nanoliter solutions (e.g., at 37 °C) within the chambers for hours without any problem of evaporation. Once assembled, the device is self‐sustainable and can be used for different application purposes. As a demonstration, the device configuration is shown to be suitable for spatiotemporal control of the inner solution conditions by light stimulation through a photomask. This method was applied for the generation of regular EmGFP (emerald green fluorescent protein) expression arrays, selective photobleaching, photopatterning of calcium concentration, and cell culture in independent microchambers. 相似文献
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Xuan Mu Wenfu Zheng Jiashu Sun Wei Zhang Xingyu Jiang 《Small (Weinheim an der Bergstrasse, Germany)》2013,9(1):9-21
Microfluidics, a toolbox comprising methods for precise manipulation of fluids at small length scales (micrometers to millimeters), has become useful for manipulating cells. Its uses range from dynamic management of cellular interactions to high‐throughput screening of cells, and to precise analysis of chemical contents in single cells. Microfluidics demonstrates a completely new perspective and an excellent practical way to manipulate cells for solving various needs in biology and medicine. This review introduces and comments on recent achievements and challenges of using microfluidics to manipulate and analyze cells. It is believed that microfluidics will assume an even greater role in the mechanistic understanding of cell biology and, eventually, in clinical applications. 相似文献
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在微流控器件的微通道内表面制备了分布可控的ZnO纳米棒阵列。先利用单分散的反相胶束借助非传统的去乳化作用在玻璃毛细管通道内壁上得到了分散很好的ZnO晶种,随后得到了ZnO纳米棒的花状簇阵列。通过调控微乳体系中的W值（水与表面活性剂的摩尔比值）得到了分散密度不同的晶种,从而在毛细管内壁上制备出了分布密度可控的ZnO纳米棒阵列,为密封的长微通道功能化改性提出了一种新的方法,经该方法改善后,可在微通道内得到纳米尺度下的一维纳米材料的特殊结构,用来设计和构筑功能化、集成化的微流控器件。 相似文献
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Paolo Falcaro Dario Buso Anita J. Hill Cara M. Doherty 《Advanced materials (Deerfield Beach, Fla.)》2012,24(24):3145-3145
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Paolo Falcaro Dario Buso Anita J. Hill Cara M. Doherty 《Advanced materials (Deerfield Beach, Fla.)》2012,24(24):3153-3168
The tuneable pore size and architecture, chemical properties and functionalization make metal organic frameworks (MOFs) attractive versatile stimuli‐responsive materials. In this context, MOFs hold promise for industrial applications and a fervent research field is currently investigating MOF properties for device fabrication. Although the material properties have a crucial role, the ability to precisely locate the functional material is fundamental for device fabrication. In this progress report, advancements in the control of MOF positioning and precise localization of functional materials within MOF crystals are presented. Advantages and limitations of each reviewed technique are critically investigated, and several important gaps in the technological development for device fabrication are highlighted. Finally, promising patterning techniques are presented which are inspired by previous studies in organic and inorganic crystal patterning for the future of MOF lithography. 相似文献
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A method for high-throughput 3D self-assembly of 2D photopatterned microstructures using railed microfluidics is presented. Vertical device patterning of heterogeneous materials requires high-level integration using conventional microelectromechanical system (MEMS) technology; however, 3D railed assembly enables easy and fast self-assembly via a fluidic axis-translation process and simple material exchange in microfluidic channels. Individually photopatterned 2D microstructures are axis-translated from in-plane to out-of-plane and fluidically self-assembled, guided by side-rails in microfluidic channels to form a 3D morphology. Since the structures are fabricated in fluidic environments, there are no fixed initial points on the channel substrate allowing fluidic horizontal stacking of erected 2D structures. The guiding mechanism of railed microfluidics enables efficient fluidic handling and deterministic 3D self-assembly of heterogeneous components such as electronic components or polymeric microstructures using only fluidic force. 相似文献
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Juan Li Xuelin Tian Alexander Pyymaki Perros Sami Franssila Ville Jokinen 《Advanced Materials Interfaces》2014,1(3)
A radial pattern with continuous topography gradient is presented, which induces a continuous inward wettability gradient and enables self‐propelling and accurate positioning of droplets to the pattern center. The effect of droplet size and wettability gradient of the pattern on the self‐mobility of droplets is investigated. The wettability gradient is found to increase towards the pattern center, enhancing the self‐motion of droplets at the inner area of the pattern. Moreover, larger droplets give rise to a larger solid‐liquid contact diameter, which helps to satisfy the self‐motion criteria that the advancing contact angle at front edge is smaller than the receding contact angle at rear edge. Consequently, a larger droplet size favors self‐motion initiated from the outer area of the pattern. The continuous topography gradient employed here allows the flexible dispensing of droplets at any place within a certain range, and avoids potential pinning defects to droplets at geometrical discontinuities. An average self‐motion velocity up to 4.0 cm/s for microliter‐sized droplets is achieved on the resultant patterned surface. 相似文献
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Electrohydrodynamic printing has gained increasing attentions to fabricate micro/nanoscale patterns in a controlled and cost-effective manner. However, most of the existing studies focus on printing tiny dried fibres, which limits its applications in high-resolution cell printing. Here we investigated the feasibility of using electrohydrodynamic printing to pattern microscale liquid filaments. Process parameters like stage moving speed and substrate resistance were optimised to stably print polyvinyl alcohol (PVA) liquid lines with the smallest line width of 37.4?μm. Complex patterns like XJTU logo with constant or variable line width were successfully printed by dynamically adjusting the moving speed. Fluorescent microparticles, with a similar diameter to living cells, were patterned in a one-by-one manner along with the PVA filaments. It is envisioned that the presented electrohydrodynamic printing method could be potentially used to high-resolution hydrogel/cell patterning for the studies of microscale cell–cell interactions or organ printing. 相似文献
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Mohammad Awashra Pinja Elomaa Tuomas Ojalehto Päivi Saavalainen Ville Jokinen 《Advanced Materials Interfaces》2024,11(1):2300596
Superhydrophilic/superhydrophobic patterned surfaces can be used to create droplet microarrays. A specific challenge with the liquids needed for various biomedical applications, as compared to pure water, is their lower surface tension and potential for contaminating the surfaces through adsorption. Here, a method is shown to create biofluid droplet microarrays using discontinuous dewetting of pure water, an oil protective layer, and finally biofluid exchange with the water droplet array. With this method, a droplet array of a viscous nucleic acid amplification solution can be formed with a low surface tension of 34 mN m−1 and a contact angle of only 76° with the used hydrophobic coating. This droplet array is applied for nucleic acid detection of SARS-CoV-2 virus using strand invasion-based amplification (SIBA) technology. It is shown that by using an array of 10 000 droplets of 50 µm diameter the limit of detection is 1 RNA copy µL−1. The results demonstrate that SIBA on droplet microarrays may be a quantitative technology. 相似文献
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《Advanced Materials Interfaces》2018,5(18)
Microcompartments are useful for the storage, controlled reaction, and release of active compounds. However, the useful lifetime of a microcompartment is generally ended when it ruptures. Here, a self‐repairing microcompartment is proposed whose dynamic polymer shell can seal after rupturing, thus extending the lifetime of the capsule and allowing for multiple mechanically‐stimulated release events. The dynamic polymer shell is created in a one‐step approach through the formation of a double network polymer in the oil phase of a double emulsion template made by microfluidics. Of the two networks, a permanently‐crosslinked network is introduced to elastically restore the shape of the microcompartment, while a dynamically‐crosslinked network forms new bonds across crack interfaces. By quantifying the timescales required for the formation of new dynamic bonds within ruptured shells, strong microcompartments with predictable strength recovery and self‐repairing capabilities are successfully prepared and used to demonstrate the possibility of mechanically‐stimulated multiple release of cargo molecules. 相似文献