冰醋酸介质中电泳共沉积制备生物玻璃/羟基磷灰石涂层

通过研究生物玻璃(bioglass，BG)微粉和羟基磷灰石(hydroxyapatite，HA)微粉在水和非水介质中的分散及带电特性，选择冰醋酸为介质，使分散在其中的BG颗粒和HA颗粒表面均带上正电荷，为电泳共沉积提供了前提条件。通过对BG颗粒和HA颗粒在冰醋酸介质中电泳共沉积以及后续低温快速热处理，在钛合金基体上成功地制备出了底层致密而表层附近多孔的BG／HA涂层。并对所制备的BG／HA涂层的力学性能和微观结构及组成进行了测试分析。     

水洗对非极性介质电泳液性能的影响研究

微量水对非极性介质分散体系的影响 ,特别是无机颜料体系的影响 ,曾经得到深入研究 ,但水对非极性有机颜料电泳液的影响 ,还有待研究。使用酸性、碱性和中性水洗涤有机颜料电泳液 ,并测试zeta电位、粒度分布以及电泳显示性能的变化 ,发现了不同pH值水洗时 ,酸性水对四氯乙烯二甲苯混合溶液联苯胺黄颜料电泳液的影响最大 ,碱性水没有不良影响 ,中性水基本没有影响。这是此类电泳液的进一步优化 ,实现电泳显示研究的基础。     

电泳沉积法制备固体电解质薄膜的沉积速率和沉积机理

研究了用电泳沉积法制备含钇８末在悬浮介质中的荷电机理;采用界面移动法测定了８ＹＳＺ粉末在有机悬浮液中的电泳淌度和ξ电势,研究了ξ电势的影响因素;考察了８ＹＳＺ粉末在有机悬浮液中的分散性和稳定性;研究了电泳沉积的动力学规律,确定了电泳沉积过程的速度控制步骤,推导出电泳沉积速率方程．利用重复沉积－烧结法制备的致密化薄膜厚度为２６～５６μｍ,硬度为４．３３ＧＰａ,在１０００℃时Ｏ－２电导率为１２．２Ｓ／ｍ,电导激活能为１．０９ｅＶ．     

阴极电泳涂料用改性超细二氧化硅的表征

对超重力沉淀法制备的超细二氧化硅以应用于阴极电泳涂料为目的，用硅烷偶联剂在水介质中进行表面改性，并对改性后的超细二氧化硅用TEM(透射电子显微镜)、粒径分析、比表面积测定、热重分析等方式进行表征。结果表明：改性后超细二氧化硅的一些特性和表面性质都发生了变化，可以用该方法对超细二氧化硅进行表面改性以满足阴极电泳涂料应用的特殊需要。     

无胶筛分毛细管电泳对SNP分型的初步研究

建立了非涂层无胶筛分毛细管电泳对多个SNP位点快速分型的方法.采用长度为20 bp和40 bp的DNA片段为实验时象,对筛分介质的种类及其质量浓度、电泳缓冲溶液的浓度及其pH值、分离电压进行了优化,确定最合适的分离条件如下:6%PDMA为筛分介质,缓冲溶液TAPS浓度为100 mmol·L-1、pH值为8,分离电压为15 kV.并在此条件下成功地对3个SNP位点进行分型.结果表明,以PDMA为筛分介质的非涂层无胶筛分毛细管电泳方法用于SNP分型是可行的,而且具有特异性强、灵敏度高、重复性好、操作简单快速、结果直观等优点.     

有机硅烷前处理与电泳涂装性能的研究

介绍了一种有机硅烷钝化液,它可以用于阴极电泳的前处理。对钝化膜的耐碱性及电泳后漆膜的附着力、耐冲击、耐腐蚀等性能进行测试,并与使用传统磷化液前处理的电泳工艺进行比较。结果表明:经有机硅烷前处理后的阴极电泳漆膜的性能符合相关行业要求,证明有机硅烷处理和阴极电泳漆完全配套,可以在汽车及相关行业中推广应用。     

外电场对苯丙氨酸在双水相体系中分配的影响

为减小电极反应和电极距离过大对于两相电泳的影响，设计了微小型多通道两相电泳设备，并以苯丙氨酸为对象开展了双水相电泳的研究工作. 较系统地研究了时间、电流密度、料液初始浓度等因素对葡聚糖–聚乙二醇–水体系中的苯丙氨酸上下相浓度比、萃取率、总传质量以及电流效率的影响. 基于分析，给出了计算最大总传质量的半经验式.     

纳米SiO2改性阴极电泳漆的制备及耐蚀性研究

以获得纳米二氧化硅改性阴极电泳漆为目的,用硅烷偶联剂在水介质中分散纳米SiO2粉体,通过分散液的吸光度来评价分散效果.然后将分散后的纳米粉体添加到阴极电泳漆中得到纳米改性电泳漆.试片经磷化-电泳涂装后得到复合涂层,并对复合涂层的耐蚀性能进行评价.金相显微观测表明,纳米改性电泳漆膜表面有较均匀的小突起,而未改性复合膜层表面比较光滑.吸水性测试表明,与未改性复合漆膜相比,改性后的复合涂层漆膜疏水性能有一定提高.耐酸、碱性试验表明,改性复合膜层的耐酸性明显优于未改性复合膜层,两种复合膜层的耐碱性都较好.     

电泳沉积法制备碳化钛膜的研究

电泳沉积法(EPD)制备薄膜具有设备简单,成本低,成膜快,被镀件(用于沉积薄膜的基体)形状不受限制,薄膜厚度均匀,并且其厚度在较大范围内可控等优点。碳化钛熔点高、硬度高、化学稳定性好,在复合材料领域具有广阔的应用前景。本文利用电泳沉积法制备出了均匀的碳化钛膜,研究了碳化钛粉末在悬浮介质中的荷电机理;考察了碳化钛粉末在有机悬浮液中的分散性和稳定性;研究了电泳沉积的动力学规律,为今后制备含碳化钛的层状复合材料打下了基础。     

电泳显示用高质量铁锰黑纳米分散系的构建

通过表面改性技术获得电泳显示用非水介质分散铁锰黑纳米粒子。在异构十二烷（Isopar L）中，选用十六烷基三甲基溴化铵（CTAB）、十二烷基硫酸钠（SDS）、(N-四乙烯四胺)聚异丁烯单丁二酰亚胺（T151）和山梨糖醇酐三油酸酯（Span 85）四种表面改性剂，通过混合球磨工艺对铁锰黑粒子进行表面修饰，研究了修饰后铁锰黑粒子在Isopar L中的粒径分布和表面荷电性质。T151对铁锰黑的修饰效果最好，原始铁锰黑粒子的平均粒径为1μm,zeta电位为-18.58mV，改性后粒径分布为117.6nm±20.5nm，平均粒径为110nm,zeta电位为-96.71mV。将改性铁锰黑粒子用作电泳显示黑粒子，制备的器件在外加电场下具有电泳响应，对比度为5.6。改性铁锰黑粒子在兼顾高分散稳定性和高带电量的同时还具有耐高温和环保的特质，这不仅为铁锰黑粒子在电泳显示应用中提供了可靠的实验基础，也为电泳黑粒子的选择提供了更多的可能性。     

双水相电泳分离氨基酸

双水相电泳是将电泳与双水相分离方法相耦合的一种新型分离技术。双水相体系因两相均富含水且界面张力低,具有良好的生物相溶性,因而在分离蛋白质等在有机溶剂中易失活和变性的有生物活性物质具有很大的优势。本文主要考察了在间歇操作过程中,双水相电泳时间、电场强度、料液初始浓度和传质面积等因素对聚乙二醇（ＰＥＧ）－葡聚糖（ＤＥＸ）－水体系中氨基酸（苯丙氨酸和这酸）的上相浓度、萃取率、和总传质量的影响,初步探讨了     

Continuous separation of proteins by hydrophilic polymer shielded multichannel flow electrophoresis

Continuous separation of protein mixtures by multichannel flow electrophoresis(MFE)wascarried out in a 5-compartment electrolyzer partitioned by membranes.Polyvinyl alcohol(PVA),polyethylene glycol 4000(PEG 4000)and polyvinylpyrrolidene K30(PVP K30)were applied to theMFE as shielding polymers to prevent protein adsorption on the polyethersulfone microfiltrationmembrane,which was used to space the central compartment and the elution compartments,duringthe electrophoresis process.The effects of polymer concentration on protein transmembrane flux wereexamined.It was found that PVA,PEG 4000 and PVP K30 greatly reduced protein adsorpton on themembrane surface.Continuous separations of bovine serum albumin(BSA)and hemoglobin(HBB)mix-ture in the presence of PEG 4000 yielded 26.6mg BSA 40.4mg HBB per hour.These results haveshown a high potential of scaling up MFE for large scale separation and purification ofbiomolecules.     

酸度对毛细管区带电泳分离蛋白质的影响分析

研究了不同酸度下的毛细管区带电泳分离牛血清白蛋白和溶菌酶混合物。比较了两种酸度下 ,毛细管电泳分离谱图、分离原理以及分离重复性的不同 ,描述了毛细管区带电泳对于蛋白质的分离机制     

Two-Phase Electrophoresis of Proteins

Abstract

Proteins have been directed into either the top or bottom phase of a polyethylene glycol/dextran aqueous two-phase system by applying an electric field perpendicular to the phase interface. Protein migration across the interface was manipulated by varying polarity, pH, electrophoresis time, field strength, and phase volume ratio. Mixtures of hemoglobin and albumin were separated by operating between isoelectric points and directing oppositely charged proteins into separate phases. Applying 50 V/cm for 2 hours to 58 mL of an equal phase volume two-phase system containing 0.2 g/L of each protein at pH 6 resulted in a bottom phase containing 99% of the hemoglobin and a top phase containing 95% of the albumin. This represents a significant improvement over the separation obtained either by partitioning in the same two-phase system with no applied field or by electrophoresis under the same conditions in homogeneous buffer. The two-phase system divides the electrophoresis device into two distinct regions, providing stability against convective mixing and facilitating product isolation.     

基于聚砜膜的透析进样毛细管电泳系统与性能评价

通过在毛细管进样端口原位相转化法制备透析膜,实现了膜预处理与毛细管电泳分离的在柱耦合的毛细管电泳方法.以多巴胺和牛血清白蛋白(BSA)为研究体系,该系统能成功拦截大分子BSA,有效避免蛋白质的干扰.膜在毛细管端口原位生成,与毛细管电泳之间几乎无死体积;与常规毛细管电泳相比,加膜后柱效和重现性损失小,出峰时间和电泳电流有...     

Effect of Poly(ethylene glycol) on Separations by Cellulose Acetate/poly(ether imide) Blend Membranes

Ultrafiltration membranes are largely being applied for macromolecular and heavy metal ion separations from aqueous streams. In this study, cellulose acetate (CA) and polyetherimide (PEI) based ultrafiltration blend membranes prepared in the absence and presence of poly(ethylene glycol) 600 (PEG 600) in various compositions were subjected to the separation of macromolecular proteins such as bovine serum albumin (BSA), egg albumin (EA), pepsin and trypsin. Toxic heavy metal ions such as Cu(II), Ni(II), Zn(II) and Cd(II) were subjected to separation by the blend membranes by complexing them with the polymeric ligand polyethyleneimine. The effects of polymer blend compositions and additive concentrations on the rejection and permeate flux of both proteins and metal ions are discussed. In general, it was found that CA/PEI blend membranes displayed higher permeate flux and lower rejection compared to pure cellulose acetate membranes at all additive concentrations. The extent of separation of proteins was found to be directly proportional to the molecular weight of the protein, while the extent of removal of metal ions depends on the affinity of metal ions to polyethyleneimine and the stability of the formed complexes.     

Multichannel Flow Electrophoresis in an Alternating Electric Field

Abstract

Investigation on the mass transfer behavior of multichannel flow electrophoresis (MFE) has confirmed the existence of concentration polarization in the separation process, which is characterized by a reduction of the relative protein migration flux corresponding to an increase in the protein concentration in the feed. The mobility of bovine serum albumin (BSA) in a gel membrane, as interpreted from its elution curve, was about one-sixth of its mobility in solution as determined by high capillary electrophoresis. This indicates that accumulation of charged protein onto the membrane surface occurs in MFE. An alternating electric field was applied instead of the steady one in MFE. The negative part of the alternating electric field reduced the polarization layer periodically. The effectiveness of this method was demonstrated by an over 40% increase of BSA migration flux at pH 6.9 in an optimized alternating electric field. The separation outputs of BSA and hemoglobin bovine blood conducted in an optimized alternating electric field at pH 6.0 were about 26 and 32% higher than their respective outputs obtained in a steady electric field. Because of its proven efficiency for reducing concentration polarization and its ease of operation, the use of an alternating electric field is a promising application in other membrane separation processes.     

毛细管电泳中的"场"、"流"分析

本文在毛细管电泳理论的基础上,分别综述了各种场和流在毛细管电泳分离中的作用以及“场”、“流”分析在毛细管电泳中的一些应用实例。对毛细管电泳分离条件进行优化的操作者会具有启发作用。     

Simultaneous depletion of albumin and immunoglobulin G by using twin affinity magnetic nanotraps

For biomarker discovery, simultaneous removal of albumin and immunoglobulin G (IgG) become more important for both concentrating other species including potential biomarkers and getting rid of their masking effect. In this study, we have proposed a covalent and photosensitive cross-linking conjugation of the biomolecules on nanostructures for the depletion of target proteins from aqueous solution and serum. The effect of concentration, pH, temperature and ionic strength on the adsorption of twin affinity nanotraps based on Cibacron Blue F3GA and Protein A were investigated. The efficiency of albumin and IgG depletion from human serum was performed using sodium dodecylsulfate–polyacrylamide gel electrophoresis.