Using estimated nutrient intake from pasture to formulate supplementary concentrate mixes for grazing dairy cows

In pasture-based dairy systems, feeding a complex concentrate mix in the parlor during milking that contains cereal grains and protein supplements has been shown to have milk production advantages over feeding straight cereal grain. This experiment had the aim of testing whether further milk production advantages could be elicited by adjusting the composition of the concentrate mix in an attempt to match the expected nutrient intake from pasture during late spring. The experiment used 96 lactating dairy cows, grazing perennial ryegrass pasture offered at a target allowance of 30 kg of dry matter/cow per day (to ground level) during late spring (mid October to November) in southeastern Australia. Cows were allocated into 3 replicates of 4 treatment groups, with 24 cows in each treatment. Each treatment group was offered 1 of 4 dietary treatments in the parlor at milking: control consisting of crushed wheat and barley grains; formulated grain mix (FGM) consisting of crushed wheat, barley, and corn grains and canola meal; designer grain mix 1 (DGM1) consisting of the same ingredients as the FGM grain mix but formulated using the CPM Dairy nutrition model to take into account the expected nutrient intake from pasture; and designer grain mix 2 (DGM2) consisting of the same ingredients as DGM1 but with canola meal replaced by urea and a fat supplement (Megalac, Volac Wilmar, Gresik, Indonesia). Concentrate mixes were offered at 8.0 kg of dry matter/cow per day, except for DGM2 cows, which were offered 7.5 kg of dry matter/cow per day. The experiment ran for a total of 28 d; after a 14-d adaptation period, nutrient intake, milk production, and body weight were measured over a 14-d measurement period. Milk yield (kg) of cows fed the FGM diet was greater than that of the control cows but was not different from that of the DGM1 and DGM2 cows. However, milk fat and protein yields (kg) were greater for cows fed the FGM diet than for all other diets. There was no difference in estimated daily pasture or total dry matter intakes between the 4 treatment groups, despite cows fed the DGM2 treatment consuming less of the concentrate mix (average 6.5 kg of dry matter/cow per day when offered 7.5 kg of dry matter/cow per day). This research has demonstrated the potential for using a nutrition model to take into account the expected nutrient intake from pasture to formulate a concentrate mix (DGM1) to achieve similar milk yields, but also highlighted the need for near real-time analyses of the pasture to be grazed so as to also capture benefits in terms of milk fat and protein yield.     

Fishmeal supplementation to grazing dairy cows in early lactation

Our objectives were to determine if grazing dairy cows would respond to fishmeal supplementation and to determine if responses could be explained by stimulation of adipose tissue lipolysis. Thirty-four multiparous Holstein cows (25+/-11 DIM) were supplemented with isonitrogenous concentrates containing either fishmeal or pelleted sunflower meal. On a dry matter (DM) basis, concentrates contained fishmeal (14.5%) or sunflower meal (24.2%), corn grain (55.6% and 50.6%), wheat bran (26.7% and 22%), a mineral-vitamin complex (2.9%) and a flavoring agent (0.3%). Concentrates were consumed at a rate of 5 kg/cow per day. Herbage allowance averaged 49.8+/-6.1 kg of DM/cow per d. Milk (26.8 vs. 25.2 kg/d), fat-corrected milk (23.9 vs. 22.2 kg/d) and milk protein yields (0.90 vs. 0.81 kg/d) were increased by fishmeal. Milk protein percentage was similar among treatments. Milk fat yield and milk and plasma urea nitrogen tended to be higher in cows fed fishmeal. Plasma glucose and nonesterified fatty acids concentrations and differences in concentrations between jugular and mammary veins were increased by fishmeal. The in vivo lipolytic response to a beta-adrenergic agent or the antilipolytic + hypoglycemic action of insulin were not affected. The higher milk production observed with fishmeal can be explained by the quantity and quality of the absorbed protein, higher glucose availability to the mammary gland, and increased lipid mobilization without change in responsiveness of the adipose tissue to lipolytic stimuli.     

Supplementation with partially hydrogenated oil in grazing dairy cows in early lactation

Effects of partially hydrogenated oil on performance, loss of body weight and body condition score, and blood metabolite and hormone concentrations were evaluated in 37 multiparous Holstein cows in grazing conditions during the first 100 d of lactation. Six additional Holstein cows, each fitted with a ruminal cannula, were allocated to a replicated 3 x 3 Latin square to evaluate effects of supplemental fat on rumen environment and pasture digestion. All cows grazed mixed pastures based on alfalfa (Medicago sativa) and orchardgrass (Dactylis glomerata L.) and received 5.4 kg/d of a basal concentrate to which 0, 0.5, or 1 kg/cow per day of partially hydrogenated oil (melting point 58 to 60 degrees C) containing 30.3, 34.9, 21.8, and 3.3% of C16:0, C18:0, C18:1, and C182, respectively, was added. Feeding 1 kg/d of supplemental fat increased fat-corrected milk from 23.4 to 26.3 kg/d, milk fat content from 3.44 to 3.78%, and milk fat yield from 0.87 to 1.03 kg/d compared to control. Milk protein percentage and yield were not affected. Cows fed 1 kg/d of fat increased the content and yield of C16:0 and C18:0 in milk compared with cows fed no added oil. Dry matter intake (DMI) from pasture decreased from 17.8 kg/d for control cows to 13.6 kg/d for cows fed 1 kg of oil, whereas DMI from concentrate was higher for cows fed 1 kg/d of fat (6.0 kg/d) than for controls (5.2 kg/d). Supplemental fat did not affect total dry matter or estimated energy intake and did not change losses of body weight or body condition scores. Plasma concentrations of nonesterified fatty acids, insulin, somatotrophin, and insulin-like growth factor-I did not differ among treatments. Concentration of plasma triglycerides was lowered from 318.5 to 271.2 mg/dl, whereas plasma cholesterol was elevated from 185.0 to 235.8 mg/dl in cows receiving 1 kg/d of supplemental fat compared with controls. Responses to lipolytic or insulin challenges were not affected by feeding oil. Supplemental fat did not affect the digestion of pasture fiber. The addition of energy in the form of partially hydrogenated fat to early lactation dairy cows fed primarily on pasture increased the yield of fat-corrected milk and milk fat content when it represented about 11% of the total metabolizable energy requirement of cows, without affecting milk protein content. The partial hydrogenation of a byproduct of the oil industry apparently prevented detrimental effects of fat supplementation on ruminal digestion.     

The effect of pasture allowance and supplementation on feed efficiency and profitability of dairy systems

Partial budgeting was used to compare income over feed costs of high-yielding Holstein cows based on data from an experiment with 4 dietary treatments arranged in a 2 x 2 factorial. The factors were low (25 kg DM/cow per day) and high (40 kg DM/cow per day) pasture allowance (PA) and supplemental grain fed at 1 kg/4 kg of milk or no supplemental grain fed. The 4 treatments were low PA unsupplemented (LPAU), low PA concentrate supplementation (LPAC), high PA unsupplemented (HPAU), and high PA concentrate supplementation (HPAC). Two management systems were modeled. The first, a fixed herd size flexible rotation length model, and the second, a flexible herd size model where rotation length was fixed. The LPAC treatment yielded the highest income over feed costs, followed by the HPAC treatment. The treatment generating the lowest income was the HPAU system. The low PA systems generated more income than did the high PA systems for equivalent supplemental feeding strategies. The results also showed that feeding supplemental grain increased the income-over-feed costs compared with systems that did not feed supplemental grain. In most treatments, comparing the fixed herd against the flexible herd models, the flexible herd size model generated higher income due to the substitution of relatively low income per hectare of hay production for higher income from milk production. There were also differences in feed conversion efficiencies for milk production due to concentrate supplementation (1.04 unsupplemented vs. 1.21 supplemented), but PA did not affect the efficiency of milk production. Neither supplementation nor PA affected the feed conversion efficiency of milk fat yield. However, the efficiency of milk protein yield was affected by concentrate supplementation (0.028 unsupplemented vs. 0.034 supplemented) but not by pasture allowance.     

Milk response to concentrate supplementation of high producing dairy cows grazing at two pasture allowances

Twenty multiparous Holstein cows (four ruminally cannulated) in five 4 x 4 Latin squares with 21-d periods were used to study the effect of concentrate supplementation when grazed at two pasture allowances. The four dietary treatments resulted from the combination of two pasture allowance targets (low, 25 vs. high, 40 kg of dry matter/cow per day) and two concentrate supplementation levels (zero vs. 1 kg of concentrate/4 kg of milk). Concentrate supplementation decreased pasture dry matter intake 2.0 kg/d at the low pasture allowance (17.5 vs. 15.5 kg/d) and 4.4 kg/d at the high pasture allowance (20.5 vs. 16.1 kg/d). Substitution rate was lower at the low pasture allowance (0.26 kg pasture/kg concentrate) than at the high pasture allowance (0.55 kg of pasture/kg of concentrate). Total dry matter intake of both supplemented treatments averaged 24.4 kg/d. Milk production of both supplemented treatments averaged 29.8 kg/d, but was increased with higher pasture allowance in the unsupplemented treatments (19.1 vs. 22.2 kg/d). Milk response to concentrate supplementation was 1.36 and 0.96 kg of milk/kg of concentrate for the low and high pasture allowances, respectively. Concentrate supplementation reduced milk fat percentage but increased milk protein percentage. Rumen pH and NH3-N concentration were decreased with concentrate supplementation. Substitution rate was likely related to both negative associative effects in the rumen (reductions in rumen pH, rate of pasture digestion, and NDF digestibility) and reductions in grazing time. The latter was more important, quantitatively explaining at least 80% of the reduction in pasture dry matter intake observed.     

Undegradable protein supplementation to early-lactation dairy cows in grazing conditions

To determine the production responses to rumen undegradable protein (RUP) feeding in grazing conditions, we fed 18 multiparous Holstein cows concentrates containing either soybean meal (SBM) or blood meal (BM) during the first 8 wk of lactation. One cow from the SBM treatment was removed because of mastitis. Six additional dairy cows in late lactation fitted with ruminal cannula were used to evaluate the rumen environment and the in situ crude protein (CP) degradability of concentrates. On a dry matter (DM) basis, concentrates contained SBM (33%) or BM (13%), corn grain (64 and 84% for SBM and BM, respectively) and a mineral-vitamin complex (3%). Concentrates were offered at a rate of 6.6 kg/d per cow and herbage allowance averaged 31 kg/d of DM per cow. The BM reduced ruminal ammonia-N levels and had no effect on ruminal pH and molar volatile fatty acid concentration. The degradable fraction (63.59 vs. 22.46%) and the rate of disappearance of the CP (9.68 vs. 1.69%/h) were greater for the SBM compared with the BM concentrate. Cows fed the BM concentrate produced more milk (29.3 vs. 24.9 kg/d) and more milk protein (0.85 vs. 0.74 kg/d) than did those fed the SBM concentrate. Milk fat yield and percentages of milk fat, lactose and protein were not affected. Forage DMI was increased by BM (17.19 vs. 13.17 kg/d per cow). The in vivo responsiveness to lipolytic stimuli were increased by BM but enhanced body weight loss or higher plasma nonesterified fatty acids concentration were not observed. Results indicated that a concentrate with a high RUP content increased milk and milk protein yields when spring pasture was the sole forage. The highest milk yield was more likely caused by increased DM than by enhanced body lipid mobilization.     

Effects of corn processing and supplemental hay on rumen environment and lactation performance of dairy cows grazing grass-legume pasture

The effect of corn processing (9 kg of dry matter/d of ground dry shelled or 9 kg of dry matter/d of steam rolled) and supplemental hay (0 or 3.2 kg of dry matter/d of alfalfa hay) on milk yield and composition, rumen environment, and starch utilization by lactating cows grazing grass-legume pasture was studied. Twelve rumen cannulated, multiparous Holstein cows in early lactation (95 d in milk), were assigned to a 4 x 4 Latin square design replicated three times. Treatments were ground shelled corn-based concentrate, ground shelled corn-based concentrate plus alfalfa hay, steam-rolled, corn-based concentrate, or steam-rolled, corn-based concentrate plus alfalfa hay. Supplements were fed in equal proportions twice daily. Cows fed steam-rolled corn tended to have higher percentage of milk protein and lower milk urea nitrogen concentrations than cows fed shelled corn. Milk yield was not affected by corn processing or hay supplementation. Intake of pasture forage but not total dry matter intake was reduced by hay supplementation. Starch plus free glucose digestibility in the total tract was not affected by grain processing; however, starch plus free glucose digestibility tended to increase with hay supplementation. Supplemental hay increased starch plus free glucose digestibility through changes in rumen digestion kinetics. Hay supplementation reduced the liquid rate of passage, and tended to reduce particulate turnover. Rumen degradability of pasture forage organic matter tended to be higher for cows fed supplemental hay. Supplemental hay in these diets had a greater impact on starch utilization than corn processing.     

Supplemental dietary protein for grazing dairy cows: effect on pasture intake and lactation performance

One hundred twenty-four cows (92 multiparous and 32 primiparous) were used to evaluate the effect of grain supplements containing high crude protein [(22.8% CP, 5.3% rumen undegradable protein (RUP), dry matter basis], moderate CP (16.6% CP, 6.1% RUP), and moderate CP with supplemental RUP (16.2% CP, 10.8% RUP) on lactation performance of Holstein cows rotationally grazing annual ryegrass-oat pastures. Supplemental protein was provided by solvent extracted soybean meal in the high CP and moderate CP supplements and as a corn gluten meal-blood meal mixture (2.8:1) in the moderate CP, high RUP supplement. Cows were blocked according to previous mature milk equivalent production and calving date (partum group; 0 d in milk or postpartum group; 21 to 65 d in milk) and randomly assigned to dietary treatments. Grain was individually fed, at approximately a 1:3 grain to milk ratio, before a.m. and p.m milkings. The study was replicated during two grazing seasons that averaged 199 d. Cows had ad libitum access to bermudagrass hay while on pasture (dry matter intake = 1.3 kg/d). Protein supplementation had no effect on study long pasture dry matter (12.7 +/- 1.0 kg/d) or total dry matter (23.9 +/- 1.2 kg/d) consumption. Protein concentration did not affect actual milk yield of either calving group (high CP vs. moderate CP); however, postpartum group cows receiving high CP grain supplements maintained greater milk fat concentrations (3.34 vs. 3.11%), which led to higher fat-corrected milk (FCM) yields than control cows receiving moderate CP grain diets (30.3 vs. 28.9 kg/d). Crude protein concentration in milk of high CP-supplemented, postpartum group cows was also higher than moderate CP cows (3.42 vs. 3.27%). Additional RUP did not increase FCM yield above that generated by moderate CP grain diets for partum (34.3 vs. 32.9 kg/d) or postpartum-group cows (28.9 vs. 28.2 kg/d). Increasing CP concentration of grain supplement did not affect milk yield of Holstein cows grazing immature winter annual pastures. Supplementing additional RUP was without benefit, indicating that in this study energy deprivation may have been the major nutritional constraint for high-producing dairy cows grazing lush pastures.     

Southeastern pasture-based dairy systems: housing,posilac, and supplemental silage effects on cow performance

This experiment tested performance and physiological responses to evaporative cooling, bovine somatotropin (bST), and supplemental silage of lactating cows grazing bermudagrass (Tifton 85; Cynodon dactylon x C. nlemfuensis cv.) pastures. Multiparous (n = 32) cows (196 d in milk) were assigned one of five treatments arranged in two replicates. Treatments were 1) cows maintained continuously on pasture with access to shade, 2) treatment 1 + bST, 3) night housing on pasture, then free-stall housing with fans and misters from 0730 to 1630 h, 4) treatment 3 + bST, and 5) treatment 4 + corn silage fed at 0.5% of body weight (dry matter basis) in the barn. A grain supplement was fed at a rate of 0.5 kg/kg of milk produced. Time spent grazing ranged from 4 to 7.2 h/d, with cows fed corn silage spending the least amount of time. Cows given bST grazed 45 min/d longer than controls, but intake of bermudagrass was unchanged. Intake of bermudagrass ranged from 7.4 to 9.5 kg/d of organic matter, with the lowest intake by cows fed corn silage. With the exception of cows fed corn silage, cows kept in a cooling barn during the day ate equivalent amounts of pasture as those given unlimited access to pasture. Production of 4% fat-corrected milk was greater by cows injected with bST (17.7 vs. 15.8 kg/d) compared with controls and tended to be greater for cows given daytime cooling compared with cows on pasture continuously (17.2 vs. 16.3 kg/d). Cows provided evaporative cooling did not lose weight compared with continually pastured cows (6.3 vs. -10.9 kg/24 d). Cows injected with bST compared with controls maintained their body weight better (2.5 vs. -7.1 kg/24 d). Cows given bST had increased concentration of plasma insulin-like growth factor-I (142 vs. 89 ng/ml), insulin (0.60 vs. 0.56 ng/ml), and nonesterified fatty acids (318 vs. 239 mEq/L). Cows given bST and those continually on pasture had greater diurnal body temperatures. Use of barn cooling systems and bST treatments as management tools provided limited improvement in performance of midlactation cows managed in pasture-based systems.     

Prepartum grain feeding and subsequent lactation forage program effects on performance of dairy cows in early lactation

One hundred fifty-six Holstein cows were balanced prior to drying off to one of three diets offered during the dry period (dry matter basis): A) forage only (50% corn silage:50% alfalfa silage), B) forage as A plus a standard dairy grain mix (1.73% calcium), and C) same as B except a low calcium grain mix (.35% calcium). Grain feeding started 3 wk prepartum. Cows from each dry treatment were assigned to one of two treatments during the first 21 d postpartum: total mixed ration (dry matter basis); 50% grain:50% corn silage; or 50% grain:25% corn silage:25% alfalfa silage. Dry period feeding program had no effect on postpartum dry matter intake, milk yield, or composition. Prepartum grain feeding resulted in increased body weight gain during the last 3 wk of the dry period. Feeding corn silage postpartum as the sole forage resulted in higher dry matter intakes (15.0 vs. 14.1 kg/d), milk production (31.3 vs. 29.7 kg/d), and less body weight loss (36 vs. 58 kg) during the first 21 d postpartum than feeding a 50% corn silage:50% alfalfa silage mixture. However, differences varied depending on dry cow feeding program. All dry cow treatments resulted in a high incidence of milk fever (11.5, 11.5, and 15.5% for A, B, and C, respectively). Dry cow rations containing as little as 50% alfalfa silage appear to predispose cows to increased incidence of milk fever.     

Crude protein and rumen undergradable protein effects on reproduction and lactation performance of Holstein cows

We conducted a study to determine the effects of excess dietary crude protein (CP) and rumen undegradable protein (RUP) on reproduction and lactation performance of Holstein cows. During each of three yearly replicates, cows were blocked by previous mature equivalent milk production and randomly assigned at calving (n = 47; partum group) or at 42 +/- 21 d postpartum (n = 134; postpartum group) to the following dietary treatments: 1) ryegrass pasture supplemented with a corn and soybean meal grain mix (high CP, moderate RUP); 2) ryegrass pasture mornings and corn silage evenings, supplemented with grain as in diet 1 (moderate CP, moderate RUP control diet), and 3) ryegrass pasture mornings and corn silage evenings, supplemented with a grain mix containing corn, soybean meal, corn gluten meal, and blood meal (moderate CP, high RUP). Dietary CP and RUP concentrations were approximately 23.1, 5.8; 17.7, 5.0; and 17.2, 6.8% of dry matter for diets 1 to 3, respectively. Plasma urea N concentrations were highest in cows fed diet 1 (25.0 mg/dl), intermediate in cows on diet 2 (20.1 mg/dl), and lowest in cows on diet 3 (18.5 mg/dl). Cows fed excess dietary protein (diet 1) exhibited lower first breeding pregnancy rates (24.1 vs. 41.0%) and lower overall pregnancy rates (53.4 vs. 75.4%) than did cows fed diet 2, increasing time nonpregnant by an average of 15.1 d per cow. Reproductive performance was similar between cows fed diets 2 and 3. Mean fat-corrected milk (FCM) yield was not affected by protein concentration (diet 1 vs. 2); however, partum group cows that received supplemental RUP (diet 3) produced more 3.5% FCM than controls in early lactation. Feeding grain diets that contained excess dietary protein impaired the reproductive performance of dairy cows grazing ryegrass.     

Invited review: production and digestion of supplemented dairy cows on pasture

Literature with data from dairy cows on pasture was reviewed to evaluate the effects of supplementation on intake, milk production and composition, and ruminal and postruminal digestion. Low dry matter intake (DMI) of pasture has been identified as a major factor limiting milk production by high producing dairy cows. Pasture DMI in grazing cows is a function of grazing time, biting rate, and bite mass. Concentrate supplementation did not affect biting rate (58 bites/min) or bite mass (0.47 g of DM/bite) but reduced grazing time 12 min/d per kilogram of concentrate compared with unsupplemented cows (574 min/d). Substitution rate, or the reduction in pasture DMI per kilogram of concentrate, is a factor which may explain the variation in milk response to supplementation. A negative relationship exists between substitution rate and milk response; the lower the substitution rate the higher the milk response to supplements. Milk production increases linearly as the amount of concentrate increases from 1.2 to 10 kg DM/d, with an overall milk response of 1 kg milk/kg concentrate. Compared with pasture-only diets, increasing the amount of concentrate supplementation up to 10 kg DM/d increased total DMI 24%, milk production 22%, and milk protein percentage 4%, but reduced milk fat percentage 6%. Compared with dry ground corn, supplementation with nonforage fiber sources or processed corn did not affect total DMI, milk production, or milk composition. Replacing ruminal degradable protein sources with ruminal undegradable protein sources in concentrates did not consistently affect milk production or composition. Forage supplementation did not affect production when substitution rate was high. Fat supplementation increased milk production by 6%, without affecting milk fat and protein content. Increasing concentrate from 1.1 to 10 kg DM/d reduced ruminal pH 0.08 and NH3-N concentration 6.59 mg/dl, compared with pasture-only diets. Replacing dry corn by high moisture corn, steam-flaked or steam-rolled corn, barley, or fiber-based concentrates reduced ruminal NH3-N concentration 4.36 mg/dl. Supplementation did not affect in situ pasture digestion, except for a reduction in rate of degradation when high amounts of concentrate were supplemented. Supplementation with energy concentrates reduced digestibility of neutral detergent fiber and intake of N but did not affect digestibility of organic matter or flow of microbial N.     

Milk fatty acid composition of cows fed a total mixed ration or pasture plus concentrates replacing corn with fat

Thirty-one Holstein cows (six ruminally cannulated) were used to evaluate milk fatty acids (FA) composition and conjugated linoleic acid (CLA) content on three dietary treatments: 1) total mixed rations (TMR), 2) pasture (Avena sativa L.) plus 6.7 kg DM/d of corn-based concentrate (PCorn), and 3) pasture plus PCorn with 0.8 kg DM/d of Ca salts of unsaturated FA replacing 1.9 kg DM/d of corn (PFat). No differences were found in total (22.4 kg/d) or pasture (18.5 kg/d) dry matter intake, ruminal pH, or total volatile fatty acids concentrations. Fat supplementation did not affect pasture neutral detergent fiber digestion. Milk production did not differ among treatments (19.9 kg/d) but 4% fat-corrected milk was lower for cows fed the PFat compared to cows fed the TMR (16.1 vs. 19.5 kg/d) primarily because of the lower milk fat percentage (2.56 vs. 3.91%). Milk protein concentration was higher for cows fed the TMR than those on both pasture treatments (3.70 vs. 3.45%). Milk from the cows fed the PCorn had a lower content of short- (11.9 vs. 10.4 g/100 g) and medium-chain (56.5 vs. 47.6 g/100 g) FA, and a higher C18:3 percentage (0.07 vs. 0.57 g/100 g) compared with TMR-fed. Cows fed the PFat had the lowest content of short- (8.85 g/100 g) and medium-chain (41.0 g/100 g) FA, and the highest of long-chain FA (51.4 g/100 g). The CLA content was higher for cows in PCorn treatment (1.12 g/100 g FA) compared with cows fed the TMR (0.41 g/100 g FA), whereas the cows fed the PFat had the highest content (1.91 g/100 g FA). Pasture-based diets increased the concentrations of long-chain unsaturated FA and CLA in milk fat. The partial replacement of corn grain by Ca salts of unsaturated FA in grazing cows accentuated these changes. However, those changes in milk FA composition were related to a depression in milk fat.     

Short communication: Effects of dietary nonstructural carbohydrates pre- and postpartum on reproduction of grazing dairy cows

The working hypothesis was that postpartum anovulatory intervals (PPAI) of grazing dairy cows are shortened by inclusion of concentrates that increase the nonstructural carbohydrate content of the transition diet. Dietary treatments were arranged as a 2 × 2 factorial, with 68 multiparous cows assigned to isoenergetic diets (114 MJ of metabolizable energy/cow per day) of pasture and pasture silage (PreP) or pasture and pasture silage supplemented with 3 kg of dry matter/cow per day a corn- and barley-based concentrate for 36 d prepartum (PreC). After calving, cows within each prepartum diet group were managed on isoenergetic diets (179 MJ of metabolizable energy/cow per day) of either pasture and pasture silage (PostP) or pasture and pasture silage supplemented with 5 kg of dry matter/cow per day a corn- and barley-based concentrate (PostC) for at least 35 d and until reestablishment of ovulatory cycles. Relative to day of calving (d 0), blood samples were collected at least weekly from d −28 to 35 and milk samples were collected twice weekly for progesterone determination to diagnose ovulatory status. The main variable of interest was PPAI, defined as the interval between calving and the first detected increase in milk progesterone (>3 ng/mL), followed by a pattern of progesterone concentrations consistent with onset of an ovulatory cycle. Subsequent mating records, pregnancy testing, and recalving data were also examined. Prepartum diet did not affect reproduction. The PPAI was 8 d shorter and the 6-wk pregnancy rate was 17% greater in PostC cows compared with PostP cows. Measured indicators of metabolic state and energy balance were poorly related to PPAI. The results support the existence of nutritional signals associated with nonstructural carbohydrates in the postpartum diet, independent of energy balance; these signals benefit the physiological mechanisms underlying the timing of first ovulation and possibly subsequent breeding performance.     

Forage Growth and Performance of Grazing Dairy Cows Supplemented with Concentrate and Chopped or Long Hay

Holstein and Brown Swiss cows were in three groups of eight in a 3 × 3 Latin square design with 4-wk periods. Cows averaged 88 d in lactation at start of trial. All cows simultaneously grazed grass and clover pastures on a rotational basis. Supplement treatments were concentrate (A), concentrate with 10% added chopped hay (B), and concentrate plus 1 kg long hay/cow daily (C). Concentrate was offered at 1 kg/3 kg 4% fat-corrected milk to cows on A and C, and 1 kg/2.7 kg to those on B. Forage growth, amounts available for grazing, and average dry matter intake were measured by clipping subsamples prior to and after each grazing and by use of cages placed in the field.Milk yields and milk fat percentages were 27.1, 3.34; 27.9, 3.32; and 28.3, 3.20 for treatments A, B, and C, respectively. Differences between A and C were significant. Milk fat percentage decreased moderately when cows first went to pasture.There were no treatment differences in fat yield, milk protein percentage, yield of fat-corrected milk, rumen fluid composition, or body weight gain.Available forage per cow exceeded 22 kg dry matter/cow daily except during one 10-d period toward the end of the trial when it amounted to 16.6 kg. Intake of dry matter from pasturage, measured by the sward cutting technique, averaged 14.5 kg./cow daily over the 12 wk.     

Effect of concentrate level on enteric methane emissions,production performance,and rumen fermentation of Jersey cows grazing kikuyu-dominant pasture during summer

The effect of concentrate feeding level on enteric CH₄ emissions from cows grazing medium quality summer pasture is yet to be investigated. Sixty multiparous Jersey cows (9 rumen-cannulated) were used in a randomized complete block design study (with the cannulated cows in a 3 × 3 Latin square design) to investigate the effect of concentrate feeding level (0, 4, and 8 kg/cow per day; as-fed basis) on enteric CH₄ emissions, production performance, and rumen fermentation of dairy cows grazing summer pasture (17 cows plus 3 cannulated cows per treatment). Enteric CH₄ emissions were measured from 11 cows per treatment group during one 7-d measurement period using the sulfur hexafluoride tracer gas technique. Pasture dry matter intake (DMI) was determined parallel with the CH₄ measurement period using TiO₂ as an external marker, and milk yield, milk composition, cow condition, and pasture pre- and postgrazing measurements were also recorded. Daily total DMI (11.2 to 15.6 kg/cow), milk yield (9.1 to 18.2 kg/cow), energy-corrected milk (ECM; 11.2 to 21.6 kg/cow), and milk lactose content (44.1 to 46.7 g/kg) increased linearly, whereas pasture DMI (11.2 to 8.4 kg/cow) decreased linearly with increasing concentrate feeding level. Daily CH₄ production (323 to 378 g/d) increased linearly due to the increase in total DMI, whereas CH₄ yield (29.1 to 25.1 g/kg of DMI) and CH₄ intensity (35.5 to 21.1 g/kg of milk yield; and 28.8 to 17.6 g/kg of ECM) decreased linearly with increasing concentrate feeding level. Diurnal ruminal pH (6.45 to 6.32) and in sacco DM and neutral detergent fiber disappearance decreased linearly. Acetic and propionic acid were unaffected by treatment, whereas butyric acid (5.21 to 6.14 mM) increased linearly and quadratically with increasing concentrate feeding level. It was concluded that a high concentrate feeding level not only increases animal efficiency but is moreover a viable CH₄ mitigation option for dairy cows grazing kikuyu-dominant pasture in late summer when pasture is inherently fibrous.     

Feed intake and milk production from three rates of concentrate for cows bred to differ in size

Two groups of Holstein cows sired by bulls that had been selected partly for transmitting ability for size averaged 525 and 570 kg weight after second calving. Cows were fed 1.0 kg concentrate to 5.0, 3.0, or 1.5 kg milk above 9.1 kg per day and forage to appetite during first and second lactations (248 lactations). Generally equal quantities of alfalfa haylage and corn silage were fed each day in weighed amounts to exceed consumption. At times haylage constituted the only forage fed. Results of feeding low (2.2 kg), medium (3.8 kg), or high (6.9 kg) concentrate dry matter daily per cow were: concentrate as a percent of total dry matter consumed, 12.2, 20.2, and 37.1%; dry matter intake from forage, 15.6, 14.5, and 11.6 kg/day; dry matter intake as percent of body weight, 3.25, 3.32, and 3.33; crude fiber as percent of dry matter consumed, 25.2, 23.6, and 20.5; crude protein as percent of dry matter consumed, 15.7, 15.4, and 15.1; total digestible nutrients consumed per day, 10.8, 11.4, and 12.2 kg; milk produced as milk/day, 18.7, 19.9, and 21.3 kg; fat-corrected milk/day, 18.6, 20.0, and 20.7 kg; fat-corrected milk as percent of body weight, 3.44, 3.68, and 3.78; and fat-corrected milk as gram per weight kg .75, 166, 178, and 183; fat content of milk, 4.00, 4.11, and 3.92%; protein content of milk, 3.49, 3.58, and 3.61%; gross efficiency of milk production, .591, .579, and .595 kg total digestible nutrients/kg fat-corrected milk; and net efficiency, .304, .308, and .332 kg total digestible nutrients/kg fat-corrected milk. Cow size, parity, and period of lactation affected most variables. These latter effects are illustrated in graphs or tables.     

Nutritional influences on the composition of milk from cows of different protein phenotypes in New Zealand

The objective of this study was to investigate the effects of contrasting nutritional regimens on milk composition from cows of different protein phenotypes. Twenty sets of seasonally calving identical twin cows that constituted five different protein phenotypes (four sets of twins per phenotype) were subjected to two nutritional treatments in crossover experiments during spring (early lactation) and summer (mid to late lactation). The phenotypes studied allowed a comparison of the AA, AB, and BB variants of both beta-lactoglobulin (beta-LG) and kappa-casein. Nutritional treatments were 1) ad libitum grazing (i.e., cows were allocated a pasture allowance of approximately 40 kg of dry matter/d per cow) plus 5 kg of a concentrate based on barley and 2) restricted grazing (pasture allowance of 20 kg of dry matter/d per cow). Milk samples were collected from each cow near the end of each 14-d treatment period and were analyzed for a detailed range of individual protein and fat constituents. Diet had significant effects on the concentrations of all milk components measured. Protein phenotype affected some protein components but not fat components. Interactions between the effects of beta-LG phenotype and diet were noted for the concentrations of some milk components. Diet and protein phenotype have important effects on the manufacturing potential of milk produced under the dairying systems of New Zealand, which rely heavily on grazing. The effects of nutrition on milk composition may depend on the beta-LG phenotype.     

Dietary structural to nonfiber carbohydrate concentration during the transition period in grazing dairy cows

A study was conducted to evaluate the potential effects of altering the proportion of dietary structural carbohydrate (SC) relative to nonfiber carbohydrate (NFC) pre- and postpartum on milk production and the circulating concentrations of hormones and metabolites. Dietary treatments were arranged as a 2 × 2 factorial, with 68 multiparous cows assigned to isoenergetic diets [114 MJ of metabolizable energy (ME)/cow per d] precalving; diets were either fresh pasture and pasture silage (PreP) or pasture and pasture silage supplemented with 3 kg dry matter (DM)/cow per d of a corn- and barley-based concentrate for 36 d prepartum (PreC). Final treatments were 13 or 32% DM NFC, respectively. After calving, cows within each prepartum diet were assigned to isoenergetic diets (179 MJ of ME/cow per d) differing in their SC and NFC content. Postcalving diets were either fresh pasture and pasture silage (PostP) or pasture and pasture silage supplemented with 5 kg DM/cow per d of a corn- and barley-based concentrate (PostC) until 35 d in milk. Final treatments were 18 and 38% DM NFC, respectively. Relative to day of calving (d 0), blood samples were collected at least weekly from d −28 to d 35. During the prepartum period, PreC cows had lower plasma urea, albumin, insulin-like growth factor-I, and Ca concentrations, but greater nonesterified fatty acid and Mg concentrations. There were no evident effects of prepartum diet on body weight or body condition score, milk yield, or milk composition. During the postpartum period, PostC cows had lower concentrations of plasma urea, β-hydroxybutyrate, and Ca, but greater concentrations of nonesterified fatty acids, glucose, insulin-like growth factor-I, and Mg. Postpartum metabolic differences in PostC cows were associated with increased milk protein production and reduced milk fat (yield and %). Results do not support a periparturient metabolic benefit to altering the SC to NFC ratio precalving, but imply an altered rumen fermentation, gluconeogenesis, and milk composition when dietary SC to NFC ratio is altered postcalving.     

Dietary Influences on Bovine Milk cis-9,trans-11-Conjugated Linoleic Acid Content

The effects of grass dry matter (DM) allowance and dietary supplements of full fat rapeseeds on levels of cis-9,trans-11 octadecadienoic (CLA) acid in bovine milk were investigated. Grass allowance of 16 kg/(cow*day) resulted in reduced (p<0.05) milk fat CLA levels (3.91 mg CLA/g fat) compared to 20 kg/(cow*day) after 19 wk treatment. CLA levels increased in milk fat from cows on a high-rapeseed-supplemented diet (p<0.001) (1650 g/(cow*day) full fat rapeseed) compared to the control (pasture) and low rapeseed (p<0.01) (825 g/(cow*day) full fat rapeseed) supplemented diets. The variation in milk fat CLA levels among individual cows over both trials was 1.5–16 mg/g.