Distinguishing mangrove species with laboratory measurements of hyperspectral leaf reflectance

As a first step in developing classification procedures for remotely acquired hyperspectral mapping of mangrove canopies, we conducted a laboratory study of mangrove leaf spectral reflectance at a study site on the Caribbean coast of Panama, where the mangrove forest canopy is dominated by Avicennia germinans, Laguncularia racemosa, and Rhizophora mangle. Using a high‐resolution spectrometer, we measured the reflectance of leaves collected from replicate trees of three mangrove species growing in productive and physiologically stressful habitats. The reflectance data were analysed in the following ways. First, a one‐way ANOVA was performed to identify bands that exhibited significant differences (P value<0.01) in the mean reflectance across tree species. The selected bands then formed the basis for a linear discriminant analysis (LDA) that classified the three types of mangrove leaves. The contribution of each narrow band to the classification was assessed by the absolute value of standardised coefficients associated with each discriminant function. Finally, to investigate the capability of hyperspectral data to diagnose the stress condition across the three mangrove species, four narrow band ratios (R ₆₉₅/R ₄₂₀, R ₆₀₅/R ₇₆₀, R ₆₉₅/R ₇₆₀, and R ₇₁₀/R ₇₆₀ where R ₆₉₅ represents reflectance at wavelength of 695nm, and so on) were calculated and compared between stressed and non‐stressed tree leaves using ANOVA.

Results indicate a good discrimination was achieved with an average kappa value of 0.9. Wavebands at 780, 790, 800, 1480, 1530, and 1550 nm were identified as the most useful bands for mangrove species classification. At least one of the four reflectance ratio indices proved useful in detecting stress associated with any of the three mangrove species. Overall, hyperspectral data appear to have great potential for discriminating mangrove canopies of differing species composition and for detecting stress in mangrove vegetation.     

Employing ground-based spectroscopy for tree-species differentiation in the Gulf Islands National Park Reserve

Airborne hyperspectral data is a promising tool to map species distribution; however, the large number of input bands can be highly correlated and potentially noisy. Ground-based spectrometer data can identify spectral regions that are optimal for species differentiation, and therefore provide a logical initial step for species mapping endeavours employing airborne hyperspectral data. This study used reflectance collected by an Analytical Spectral Devices (ASD) spectrometer to differentiate between tree species common to the Canadian Gulf Islands. Baseline ASD reflectance and its derivatives were used as input for forward stepwise discriminant analyses to identify wavelengths that minimize within-species variance while maximizing between-species variance. Identified wavelengths were then used as input for normal discriminant analyses, which confirmed through cross-validation classifications that, at the leaf scale, species could be differentiated with an overall accuracy > 98% and individual accuracies > 85% using 40 optimal wavelengths. Accuracies slightly decreased when using derivatives, but only for certain species. Results indicate that wavelengths in the ranges 501–550, 681–740 and 1401–1800 nm exhibited the most significance. The selected bands form the basis of ongoing mapping efforts using airborne hyperspectral imagery.     

Atmospheric optical depth effects on angular anisotropy of plant canopy reflectance

Abstract

A field experiment was conducted to determine whether changes in atmospheric aerosol optical depth would effect changes in bi-directional reflectance distributions of vegetation canopies. Measurements were made of the directionally reflected radiance distributions of two pasture grass canopies (same species, different growth forms) and one soya bean plant canopy under different sky irradiance distributions, which resulted from a variation in aerosol optical depth. The reflected radiance data were analysed in the solar principal plane in two narrow spectral bands, one visible (662 nm) and one infrared (826 nm). The observed changes in reflectance for both wavelengths from irradiance distribution variation is interpreted to be due largely to changes in the percentage of shadowed area viewed by the sensor for the incomplete canopies (pasture grass). For the complete coverage vegetation canopy (soya bean) studied, the effects of specular reflection and the increased diffuse irradiance penetration into the canopy are concluded to be primary physical mechanisms responsible for reflectance changes. Observed reflectivities were found to be lower on a hazy day (higher optical depth with a greater diffuse fraction) than on a clear day, with solar zenith angles at about 58^° on both days, for full-coverage soya bean canopies. The reduced reflectance most likely results from a diminished specular reflection and a greater diffuse radiation penetration into the canopy, which effects an increased energy absorption at large solar zenith angles. The opposite was true for fractional coverage grass canopies at solar zenith angles of about 56^° since the shadowing was less on the hazy day and, therefore, the soil/litter background was more fully illuminated. In the near-infrared waveband the changes in reflectance are much less than in the visible and, therefore, normalized difference vegetation index values differ substantially under clear and hazy sky conditions for the same vegetation canopy conditions. Thus, the influence of atmospheric optical depth must be considered for accurate remote sensing and in situ data interpretation.     

Discriminating sodium concentration in a mixed grass species environment of the Kruger National Park using field spectrometry

Sodium has been found to be a scarce element needed and sought by mammals. To date, most geophagical studies have mainly concentrated on sodium in the soil with limited attention being given to the plant component. Mapping foliar sodium distribution is important to understand wildlife feeding patterns and distribution. In this study, we established whether remote sensing can be used to discriminate different levels of sodium concentration in grass. A GER 3700 spectrometer was used to measure spectral reflectance of grass in the field. Since savannah rangelands are characterized by mixed grass species, we first established the variation of foliar sodium concentration in different grass species and tested for possible effects of species–sodium interaction on spectral reflectance. Our results showed statistically significant differences between the mean reflectance for the low and medium sodium classes. No significant differences were observed between reflectance in the high sodium class and the lower classes. However, there was a significant interaction between sodium classes and species in influencing reflectance. We concluded that, in combination with knowledge of grass species distribution, hyperspectral remote sensing may be useful in classifying foliar sodium concentration in savannah rangelands. This may help to understand the distribution of mammals in some African savannahs where mineral nutrient availability is limiting.     

Exploring spectral discrimination of grass species in African rangelands

The spectral reflectance from eight species of rangeland grasses in the Masai Mara Nature Reserve, Kenya, was measured using a laboratory-based spectrometer. There were statistically significant differences in the spectral reflectance between species-a result which is encouraging for future work on identifying, classifying, mapping and monitoring rangeland ecosystems from hyperspectral imagery. To date, hyperspectral imagery has been available only through airborne scanners, but the European Space Agency and the United States' National Aeronautics and Space Administration (NASA) both plan satellite missions. The second part of this paper describes the acquisition and analysis of hyperspectral data (CASI) coincident with ground plots. In these plots, the mix of grass species varied from pure (monospecific) patches through to mixes of four to five different species. Evidence is presented indicating that some species may be identified on the image, based on the laboratory-obtained spectra.     

Evaluation of hyperspectral data for pasture estimate in the Brazilian Amazon using field and imaging spectrometers

We used two hyperspectral sensors at two different scales to test their potential to estimate biophysical properties of grazed pastures in Rondônia in the Brazilian Amazon. Using a field spectrometer, ten remotely sensed measurements (i.e., two vegetation indices, four fractions of spectral mixture analysis, and four spectral absorption features) were generated for two grass species, Brachiaria brizantha and Brachiaria decumbens. These measures were compared to above ground biomass, live and senesced biomass, and grass canopy water content. The sample size was 69 samples for field grass biophysical data and grass canopy reflectance. Water absorption measures between 1100 and 1250 nm had the highest correlations with above ground biomass, live biomass and canopy water content, while ligno-cellulose absorption measures between 2045 and 2218 nm were the best for estimating senesced biomass. These results suggest possible improvements on estimating grass measures using spectral absorption features derived from hyperspectral sensors. However, relationships were highly influenced by grass species architecture. B. decumbens, a more homogeneous, low growing species, had higher correlations between remotely sensed measures and biomass than B. brizantha, a more heterogeneous, vertically oriented species. The potential of using the Earth Observing-1 Hyperion data for pasture characterization was assessed and validated using field spectrometer and CCD camera data. Hyperion-derived NPV fraction provided better estimates of grass surface fraction compared to fractions generated from convolved ETM+/Landsat 7 data and minimized the problem of spectral ambiguity between NPV and Soil. The results suggest possible improvement of the quality of land-cover maps compared to maps made using multispectral sensors for the Amazon region.     

Conifer species recognition: Effects of data transformation

In situ hyperspectral data obtained with a high spectral resolution radiometer were analysed for identification of six conifer species. Hyperspectral data were measured in the summer and late fall seasons at 15-20 cm above portions of tree canopies from both the sunlit and shaded sides. An artificial neural network algorithm was applied for identification purposes. Six types of transformation were applied to the hyperspectral reflectance data ( R ), preprocessed with a simple smoothing, followed by band aggregation. These include log( R ), first derivative of R, first derivative of log( R ), normalized R, first derivative of normalized R, and log(normalized R ). First derivative of log( R ) and first derivative of normalized R resulted in best species recognition accuracies with greater than 90% average accuracies, more than 20% greater than the average accuracy obtained from the pre-processed hyperspectral data. The effect of hyperspectral data taken from the shade sides of tree canopies can be minimized by applying normalization or by taking the derivatives after applying a logarithm to the pre-processed data. We found that a big difference in solar angle did not cause a noticeable difference in accuracies of species recognition.     

Comparison of two hyperspectral imaging and two laser-induced fluorescence instruments for the detection of zinc stress and chlorophyll concentration in bahia grass (Paspalum notatum Flugge.)

Bahia grass (Paspalum notatum Flugge.) plants were grown in silica sand and irrigated daily with one of five levels of Zn (0, 0.5, 25, 50, or 100 mg l⁻¹) to determine the effects of the heavy metal on the growth and development of plant canopies. Healthy and stressed plants were measured with two hyperspectral imagers, laser-induced fluorescence spectroscopy (LIFS), and laser-induced fluorescence imaging (LIFI) systems in order to determine if the four handheld remote sensing instruments were equally capable of detecting plant stress and measuring canopy chlorophyll levels in bahia grass. Symptoms of bahia grass plants grown at deficient (0 mg l⁻¹) or toxic (25, 50, or 100 mg l⁻¹) concentrations of Zn were dominated by leaf chlorosis and plant stunting. Leaf fresh weight, leaf dry weight, CO₂ assimilation, total chlorophyll, and leaf thickness followed (+) quadratic models in which control plants (0.5 mg l⁻¹ Zn) exhibited higher responses than plants grown at either deficient or toxic levels of Zn. Normalized difference vegetation index [NDVI=(NIR−Red)/(NIR+Red)] and ratio vegetation index [RVI=R₇₅₀/R₇₀₀, in which R denotes reflectance] values were calculated for calibrated digital images from both hyperspectral imagers. The NDVI and RVI values from both hyperspectral imagers were fit best by (+) quadratic models when treatments were constrained between 0 and 100 mg l⁻¹ Zn, but were fit best by linear regression models with (−) slopes when treatments were constrained between 0.5 and 100 mg l⁻¹ Zn. Furthermore, both NDVI and RVI algorithms were effective in predicting the concentrations of chlorophyll in canopies of bahia grass grown at the various levels of Zn. In contrast, red/far-red (R/FR) fluorescence ratios estimated from leaf fluorescence values measured with the LIFS and LIFI instruments were fit best by (−) quadratic models when treatments were constrained between 0 and 100 mg l⁻¹ Zn, but were fit best by linear regression models with (+) slopes when treatments were constrained between 0.5 and 100 mg l⁻¹ Zn. A series of regression analyses were conducted among plant biometric, biochemical, and leaf anatomical parameters (treated as independent variables) and the remote sensing algorithms, NDVI, RVI, blue/green (BL/GR), and R/FR (treated as dependant variables). In general, residuals were significantly higher for NDVI and RVI models compared to the BL/GR and R/FR models indicating that the NDVI and RVI algorithms were able to measure total chlorophyll and plant biomass more accurately than the BL/GR and R/FR algorithms. However, unique capabilities of LIFS and LIFI instruments continue to argue for the development of laser-induced fluorescence remote sensing technologies.     

Correlation between soil apparent electroconductivity and plant hyperspectral reflectance in a managed wetland

The apparent electrical conductivity (σ_a) of soil is influenced by a complex combination of soil physical and chemical properties. For this reason, σ_a is proposed as an indicator of plant stress and potential community structure changes in an alkaline wetland setting. However, assessing soil σ_a is relatively laborious and difficult to accomplish over large wetland areas. This work examines the feasibility of using the hyperspectral reflectance of the vegetation canopy to characterize the σ_a of the underlying substrate in a study conducted in a Central California managed wetland. σ_a determined by electromagnetic (EM) inductance was tested for correlation with in-situ hyperspectral reflectance measurements, focusing on a key waterfowl forage species, swamp timothy (Crypsis schoenoides). Three typical hyperspectral indices, individual narrow-band reflectance, first-derivative reflectance and a narrow-band normalized difference spectral index (NDSI), were developed and related to soil σ_a using univariate regression models. The coefficient of determination (R ²) was used to determine optimal models for predicting σ_a, with the highest value of R ² at 2206 nm for the individual narrow bands (R ²?=?0.56), 462 nm for the first-derivative reflectance (R ²?=?0.59), and 1549 and 2205 nm for the narrow-band NDSI (R ²?=?0.57). The root mean squared error (RMSE) and relative root mean squared error (RRMSE) were computed using leave-one-out cross-validation (LOOCV) for accuracy assessment. The results demonstrate that the three indices tested are valid for estimating σ_a, with the first-derivative reflectance performing better (RMSE?=?30.3 mS m^?1, RRMSE?=?16.1%) than the individual narrow-band reflectance (RMSE?=?32.3 mS m^?1, RRMSE?=?17.1%) and the narrow-band NDSI (RMSE?=?31.5 mS m^?1, RRMSE?=?16.7%). The results presented in this paper demonstrate the feasibility of linking plant–soil σ_a interactions using hyperspectral indices based on in-situ spectral measurements.     

Post senescent grass canopy remote sensing

Analysis of in situ collected spectral reflectance data from a dormant or senescent grass canopy showed a direct relationship existed between spectral reflectance and biomass for the 0.50–0.80 μm spectral region. The data, collected four weeks after the end of the growing season, indicated that post senescent remote sensing of grass canopy biomass is possible and helps to elucidate the spectral contribution of recently dead vegetation in mixed live/dead canopy situations.     

The effect of clumping on canopy scattering and its directional properties: a model simulation using spectral invariants

The spectral invariants theory predicts that the bidirectional reflectance factor (BRF) of a vegetation canopy can be expressed in terms of the canopy interceptance (i₀), the recollision probability (p), and the directional escape probability (ρ). These spectral invariant parameters together form a novel canopy structural parameter – the directional area scattering factor (DASF). The DASF can be retrieved from remotely sensed hyperspectral imagery and has been found to be useful, e.g. for the separation of tree species. The spectral invariants theory, however, does not provide an interpretation of which specific canopy structural properties are captured by the DASF. In this study, we examined the possible link between the DASF and the canopy clumping index (β). A simple model was designed to simulate the effect of β on canopy first order scattering, which was assumed to govern the directional behaviour of the DASF. The model is based on a modified spectral invariants approach, where the assumption of constant p is relaxed so that the first order recollision probability (p₁) and single scattering are calculated separately, and canopy BRF is expressed as the sum of the first and multiple order components. Simulations were performed on model canopies, where radiation penetration is described using a traditional statistical approach but allowing non-random foliage distributions caused by clumping. The results indicated a strong dependency between the modelled DASF and the canopy clumping index.     

Close-range remote sensing of aquatic macrophyte vegetation cover

This study used ground-based hyperspectral radiometry to examine variations in visible and near-infrared spectral reflectance of spatterdock (Nuphar polysepalum Engelm.) as a function of vegetation cover. Sites were sampled in Swan Lake in Grand Teton National Park, Wyoming, using a 512-band spectroradiometer to measure reflectance over the range 326.5-1055.3nm (visible-nearinfrared) and simultaneous estimates of spatterdock cover. Linear correlations between spatterdock cover and spectral reflectance were statistically significant at the 0.05 significance level in two specific ranges of the spectrum: 518-607 nm; and 697-900nm. Predictability of spatterdock cover using spectral variables was best using an NDVI transformation of the data in a non-linear equation (r ² = 0.95).     

A new technique for extracting the red edge position from hyperspectral data: The linear extrapolation method

The position of the inflexion point in the red edge region (680 to 780 nm) of the spectral reflectance signature, termed the red edge position (REP), is affected by biochemical and biophysical parameters and has been used as a means to estimate foliar chlorophyll or nitrogen content. In this paper, we report on a new technique for extracting the REP from hyperspectral data that aims to mitigate the discontinuity in the relationship between the REP and the nitrogen content caused by the existence of a double-peak feature on the derivative spectrum. It is based on a linear extrapolation of straight lines on the far-red (680 to 700 nm) and NIR (725 to 760 nm) flanks of the first derivative reflectance spectrum. The REP is then defined by the wavelength value at the intersection of the two lines. The output is a REP equation, REP = − (c₁ − c₂) / (m₁ − m₂), where c₁ and c₂, and m₁ and m₂ represent the intercepts and slopes of the far-red and NIR lines, respectively. Far-red wavebands at 679.65 and 694.30 nm in combination with NIR wavebands at 732.46 and 760.41 nm or at 723.64 and 760.41 nm were identified as the optimal combinations for calculating nitrogen-sensitive REPs for three spectral data sets (rye canopy, and maize leaf and mixed grass/herb leaf stack spectra). REPs extracted using this new technique (linear extrapolation method) showed high correlations with a wide range of foliar nitrogen concentrations for both narrow and wider bandwidth spectra, being comparable with results obtained using the traditional linear interpolation, polynomial and inverted Gaussian fitting techniques. In addition, the new technique is simple as is the case with the linear interpolation method, but performed better than the latter method in the case of maize leaves at different developmental stages and mixed grass/herb leaves with a low nitrogen concentration.     

Detection of stressed oil palms from an airborne sensor using optimized spectral indices

Existing vegetation indices and red-edge techniques have been widely used for the assessment of vegetation status and vegetation health from remote-sensing instruments. This study proposed and applied optimized Airborne Imaging Spectrometer for Applications (AISA) airborne hyperspectral indices in assessing and mapping stressed oil palm trees. Six vegetation indices, four red-edge techniques, a standard supervised classifier and three optimized AISA spectral indices were compared in mapping diseased oil palms using AISA airborne hyperspectral imagery. The optimized AISA spectral indices algorithms used newly defined reflectance values at wavelength locations of 734 nm (near-infrared (NIR)) and 616 nm (red). The selection of these two bands was based on laboratory statistical analysis using field spectroradiometer reflectance data. These two bands were then applied to the AISA airborne hyperspectral imagery using the three optimized algorithms for AISA data. The newly formulated AISA hyperspectral indices were D2 = R ₆₁₆/R ₇₃₄, normalized difference vegetation index a (NDVIa)?=?(R ₇₃₄ – R ₆₁₆)/(R ₇₃₄?+?R ₆₁₆) and transformed vegetation index a (TVIa)?=?((NDVIa?+?0.5)/(abs (NDVIa?+?0.5))?×?[abs (NDVIa?+?0.5)]^1/2. The classification results from the optimized AISA hyperspectral indices were compared with the other techniques and the optimized AISA spectral indices obtained the highest overall accuracy. D2 and NDVIa obtained 86% of overall accuracy followed by TVIa with 84% of overall accuracy.     

Detecting near-surface moisture stress in Sphagnum spp.

Estimating near-surface moisture conditions from the reflectance spectra (400-2500 nm) of Sphagnum moss offers great opportunities for the use of remote sensing as a tool for large-scale detailed monitoring of near-surface peatland hydrological conditions. This article investigates the effects of changes in near-surface and surface moisture upon the spectral characteristics of Sphagnum moss. Laboratory-based canopy reflectance data were collected from two common species of Sphagnum subjected to drying and subsequent rewetting. Several spectral indices developed from the near infra-red (NIR) and shortwave infra-red (SWIR) liquid water absorption bands and two biophysical indices (REIP and the chlorophyll index) were correlated with measures of near-surface moisture. All spectral indices tested were significantly correlated with near-surface moisture (with r between 0.27 and 0.94). The strongest correlations were observed using indices developed from the NIR liquid water absorption features (fWBI₉₈₀ and fWBI₁₂₀₀). However, a hysteretic response was observed in both NIR indices when the canopies were re-hydrated, a finding which may have implications for the timing of remote sensing image acquisition. The Moisture Stress Index (MSI), developed from the SWIR liquid water absorption feature also showed strong correlations with near-surface wetness although the range of moisture conditions over which the index was able to detect change was highly dependent on Sphagnum species. Of the biophysical spectral indices tested (REIP and the chlorophyll index), the most significant relationships were observed between the chlorophyll index and near-surface wetness. All spectral indices tested were species specific, and this is attributed to differences in canopy morphology between Sphagnum species. The potential for developing estimations of surface and near-surface hydrological conditions across northern peatlands using remote sensing technology is discussed.     

Steady-state chlorophyll a fluorescence detection from canopy derivative reflectance and double-peak red-edge effects

A series of experiments carried out in a controlled environment facility to induce steady-state chlorophyll a fluorescence variation demonstrate that natural fluorescence emission is observable on the derivative reflectance spectra as a double-peak feature in the 690-710 nm spectral region. This work describes that the unexplained double-peak feature previously seen on canopy derivative reflectance is due entirely to chlorophyll fluorescence (CF) effects, demonstrating the importance of derivative methods for fluorescence detection in vegetation. Measurements were made in a controlled environmental chamber where temperature and humidity were varied through the time course of the experiments in both short- and long-term trials using Acer negundo ssp. californium canopies. Continuous canopy reflectance measurements were made with a spectrometer on healthy and stressed vegetation, along with leaf-level steady-state fluorescence measurements with the PAM-2000 Fluorometer during both temperature-stress induction and recovery stages. In 9-h trials, temperatures were ramped from 10 to 35 °C and relative humidity adjusted from 92% to 42% during stress induction, returning gradually to initial conditions during the recovery stage. Canopy reflectance difference calculations and derivative analysis of reflectance spectra demonstrate that a double-peak feature created between 688, 697 and 710 nm on the derivative reflectance is a function of natural steady-state fluorescence emission, which gradually diminished with induction of maximum stress. Derivative reflectance indices based on this double-peak feature are demonstrated to track natural steady-state fluorescence emission as quantified by two indices, the double-peak index (DPi) and the area of the double peak (A_dp). Results obtained employing these double-peak indices from canopy derivative reflectance suggest a potential for natural steady-state fluorescence detection with hyperspectral data. Short- and long-term stress effects on the observed double-peak derivative indices due to pigment degradation and canopy structure changes were studied, showing that both indices are capable of tracking steady-state fluorescence changes from canopy remote sensing reflectance.     

Uncertainties in fPAR estimation of grass canopies under different stress situations and differences in architecture

The fraction of intercepted photosynthetic active radiation (fPAR) is a key variable used by the Monteith model to estimate the net primary productivity (NPP). This variable can be assessed by vegetation indices (VIs) derived from spectral remote sensing data but several factors usually affect their relationship. The objectives of this work were to analyse the fPAR dynamics and to describe the relationships between fPAR and several indices (normalized difference vegetation index (NDVI), optimized soil adjusted vegetation index (OSAVI), Green NDVI (GNDVI), visible atmospherically resistant index (VARI) green, VIgreen and red edge position (REP)) under different water and nutrient treatments for two species with different canopy architectures. Two C₃ grass species with differences in leaf orientation (planophile and erectophile) were cultivated from seeds in pots. Four treatments were applied combining water and nitrogen availability. Every week, canopy reflectance and fPAR were measured. Aerial biomass was clipped to estimate final above-ground production for each species and treatment. Starting from reflectance values, the indices were calculated. Planophile species have a steeper (but not significantly) slope in VIs–fPAR relationships than the erectophile species. Water and nutrient deficiencies treatment showed no relationship with fPAR in any spectral index in the erectophile species. In the other species, this treatment showed significant relationship according to the index used. Analysing each species individually, treatments did not modify slopes except in one case (planophile species between both treatments with high nitrogen but differing in water availability). Among indices, GNDVI was the best estimator of fPAR for both species, followed by NDVI and OSAVI. Inaccurate results may be obtained from commonly reported spectral relationships if plants' stress factors are not taken into account.     

Broadleaf species recognition with in situ hyperspectral data

Timely and accurate identification of tree species by spectral methods is crucial for forest and urban ecological management. In this study, a total of 394 reflectance spectra (between 350 and 2500 nm) from foliage branches or canopy of 11 important urban forest broadleaf species were measured in the City of Tampa, Florida, USA with a spectrometer. The 11 species include American elm (Ulmus americana), bluejack oak (Quercus incana), crape myrtle (Lagerstroemia indica), laurel oak (Q. laurifolia), live oak (Q. virginiana), southern magnolia (Magnolia grandiflora), persimmon (Diospyros virginiana), red maple (Acer rubrum), sand live oak (Q. geminata), American sycamore (Platanus occidentalis), and turkey oak (Q. laevis). A total of 46 spectral variables, including normalized spectra, derivative spectra, spectral vegetation indices, spectral position variables, and spectral absorption features were extracted and analysed from the in situ hyperspectral measurements. Two classification algorithms were used to identify the 11 broadleaf species: a nonlinear artificial neural network (ANN) and a linear discriminant analysis (LDA). An analysis of variance (ANOVA) indicates that the 30 selected spectral variables are effective to differentiate the 11 species. The 30 selected spectral variables account for water absorption features at 970, 1200, and 1750 nm and reflect characteristics of pigments and other biochemicals in tree leaves, especially variability of chlorophyll content in leaves. The experimental results indicate that both classification algorithms (ANN and LDA) have produced acceptable accuracies (overall accuracy from 86.3% to 87.8%, kappa from 0.83 to 0.87) and have a similar performance for classifying the 11 broadleaf species with input of the 30 selected spectral variables. The preliminary results of identifying the 11 species with the in situ hyperspectral data imply that with current remote sensing techniques, including high spatial and spectral resolution data, it is still difficult but possible to identify similar species to such 11 broadleaf species with an acceptable accuracy.     

Mapping the effects of water stress on Sphagnum: Preliminary observations using airborne remote sensing

Remote sensing of near-surface hydrological conditions within northern peatlands has the potential to provide important large-scale hydrological information regarding ecological and carbon-balance processes occurring within such systems. This article details how field knowledge of the spectral properties of Sphagnum spp., airborne remote sensing data and a range of image analysis approaches, may be combined to provide a suitable proxy for near-surface wetness. Co-incident field and airborne remote sensing data were acquired in May and September 2002 over an important UK raised bog (Cors Fochno). A combination of laboratory-tested NIR and SWIR water-based and biophysical spectral reflectance indices were applied to field and airborne reflectance spectra of Sphagnum pulchrum to elucidate changes in near-surface moisture conditions. Field results showed significant correlations between water-based indices (moisture stress index (MSI) and floating water band indices (fWBI₉₈₀ and fWBI₁₂₀₀₎)) and measures of both near-surface volumetric moisture content (VMC) and water-table position. Spectral indices formulated from the NIR (fWBI₉₈₀ and fWBI₁₂₀₀) proved to be the most useful for indicating near-surface wetness across the widest range of moisture conditions because of their ability to penetrate deeper into the Sphagnum canopy. Correlations between a biophysical index based upon chlorophyll content and both hydrological measures were not significant, possibly due to relatively high levels of surface wetness at the field site in both May and September. S. pulchrum lawns were successfully located and mapped from airborne imagery using the mixed tuned match filtering (MTMF) algorithm. Importantly, MSI derived from airborne data was significantly correlated with both field moisture and the water-table position. Relationships between measures of near-surface wetness and the MSI for naturally heterogeneous canopies were, however, found to be weaker for airborne imagery than for associated field data. This is likely to be a result of the formulation of the MSI itself and the possible preferential detection of “wetter” pixels within the imagery. This effectively reduced the ability of MSI to detect subtle changes in near-surface wetness under high moisture conditions, but would not impede the use of the index under drier conditions. Results from the field data suggest that indices formulated from the NIR may be more suitable for detailed estimations of near-surface and surface wetness at the landscape-scale although reliable hyperspectral data are required to test fully the performance of such indices. The relative merits of using such an approach to determine near-surface hydrological conditions across entire peatland complexes are also discussed.     

Estimating leaf nitrogen concentration in heterogeneous crop plants from hyperspectral reflectance

The estimation of leaf nitrogen concentration (LNC) in crop plants is an effective way to optimize nitrogen fertilizer management and to improve crop yield. The objectives of this study were to (1) analyse the spectral features, (2) explore the spectral indices, and (3) investigate a suitable modelling strategy for estimating the LNC of five species of crop plants (rice (Oryza sativa L.), corn (Zea mays L.), tea (Camellia sinensis), gingili (Sesamum indicum), and soybean (Glycine max)) with laboratory-based visible and near-infrared reflectance spectra (300–2500 nm). A total of 61 leaf samples were collected from five species of crop plant, and their LNC and reflectance spectra were measured in laboratories. The reflectance spectra of plants were reduced to 400–2400 and smoothed using the Savitzky–Golay (SG) smoothing method. The normalized band depth (NBD) values of all bands were calculated from SG-smoothed reflectance spectra, and a successive projections algorithm-based multiple linear regression (SPA-MLR) method was then employed to select the spectral features for five species. The SG-smoothed reflectance spectra were resampled using a spacing interval of 10 nm, and normalized difference spectral index (NDSI) and three-band spectral index (TBSI) were calculated for all wavelength combinations between 400 and 2400 nm. The NDSI and TBSI values were employed to calibrate univariate regression models for each crop species. The leave-one-out cross-validation procedure was used to validate the calibrated regression models. Study results showed that the spectral features for LNC estimation varied among different crop species. TBSI performed better than NDSI in estimating LNC in crop plants. The study results indicated that there was no common optimal TBSI and NDSI for different crop species. Therefore, we suggest that, when monitoring LNC in heterogeneous crop plants with hyperspectral reflectance, it might be appropriate to first classify the data set considering different crop species and then calibrate the model for each species. The method proposed in this study requires further testing with the canopy reflectance and hyperspectral images of heterogeneous crop plants.