隐脉假卫矛中黄酮类化学成分研究

综合运用硅胶柱层析、Sephadex LH-20凝胶柱层析、ODS反相柱层析及制备型高效液相等现在色谱分离技术,对卫矛科假卫矛属植物隐脉假卫矛Microtropis obscurinervia枝叶的化学成分进行系统的分离和纯化。根据化合物的理化性质和波谱数据,并通过与文献对照,鉴定了从隐脉假卫矛枝叶95%乙醇提取物的石油醚萃取部位中分离得到8个黄酮类化合物,分别鉴定为:芹菜素(1)、木犀草素(2)、香叶木素(3)、鼠李素(4)、5,6-二羟基-7,4′-二甲氧基黄酮(5)、7-羟基-5,3′,4′-三甲氧基黄酮(6)、5,7,3′,4′-四甲氧基黄酮(7)和5-羟基-6,7,8,3',4'-五甲氧基黄酮(8)。本研究首次对隐脉假卫矛中的化学成分进行了系统研究,所有化合物均为首次从假卫矛属植物中分离得到。     

三脉紫菀化学成分的研究

目的：研究三脉紫菀的化学成分。方法：应用正、反相硅胶柱色谱法进行分离纯化,根据理化性质和波谱数据鉴定化合物结构。结果：从三脉紫菀甲醇提取物中分离得到了5个化合物,分别鉴定为：β-谷甾醇（1）、柚皮素（2）、木栓酮（3）、表木栓醇（4）、3＇,4＇,5,7-四羟基双氢黄酮（5）。结论：化合物2-5为首次从该植物中分离得到...     

悬钩子属植物三萜类化学成分及波谱特征研究进展

对悬钩子属植物中三萜类成分研究进行综述,并对其波谱特征进行归纳总结。从悬钩子属植物中已分离得35个三萜类化合物,其中有9个为齐墩果型,26个为乌索烷型,这些化合物的基本骨架、取代基类型及位置均有其波谱特征。     

齿叶黄皮中生物碱类化学成分研究

综合运用正相硅胶柱层析、ODS反相硅胶柱层析、Sephadex LH-20凝胶柱层析以及制备型HPLC等现代色谱分离技术,对芸香科黄皮属植物齿叶黄皮(Clausena dunniana)枝叶90%乙醇提取物的石油醚萃取部位中的化学成分进行系统分离与纯化。结合化合物的理化性质和多种现代波谱技术,并通过与文献中报道的数据对照,鉴定了分离得到8个生物碱类化合物,分别为cinnamamide(1)、N-methyl-cinnamamide(2)、dihydroalatamide(3)、zeta-clausenamide(4)、lansine(5)、3-formyl carbazole(6)、3-formyl-6-methoxy carbazole(7)和6-methoxycarbazole-3-carboxylate(8)。化合物1~8均为首次从齿叶黄皮中分离得到。     

齿叶黄皮中香豆素类化学成分研究

综合运用硅胶柱层析、ODS柱层析、Sephadex LH-20凝胶柱层析以及制备型HPLC等现代色谱分离技术,对芸香科黄皮属植物齿叶黄皮(Clausena dunniana)枝叶的化学成分进行系统分离与纯化。结合理化性质和多种现代波谱鉴定技术,并通过与文献报道的数据对照,鉴定了从齿叶黄皮枝叶90%乙醇提取物的石油醚萃取部位中分离得到8个香豆素类化合物的化学结构,分别为6-羟基-8-甲氧基-香豆素(1)、秦皮素(2)、异秦皮素(3)、佛手柑内酯(4)、花椒毒素(5)、异欧前胡素(6)、九里香酮(7)和蛇床子素(8)。以上所有化合物均为首次从齿叶黄皮中分离得到。     

云南产茯苓的化学成分研究

对云南产茯苓的化学成分进行研究,寻找具有重要生物活性及药用前景的天然产物。将茯苓干燥粉末进行水蒸气回流提取,浓缩,依次用石油醚、乙酸乙酯萃取。将石油醚及乙酸乙酯萃取物经正相和反相硅胶柱、制备型HPLC等色谱方法进行分离纯化,并根波谱学数据进行结构解析及确定。结果表明:从茯苓的石油醚及乙酸乙酯萃取物中分离得到6个化合物,分别鉴定为:亚油酸(1)、茯苓新酸B(2)、茯苓新酸G(3)、Poriacosones A(4)3-氢化-松苓酸(5)和依布里酸(6)。经查阅文献可知,化合物(1)为首次从该植物中分离得到。     

迷迭香化学成分研究

为研究迷迭香的化学成分,采用溶剂w(CH3CH2OH)=95%热浸法提取,再依次用石油醚、乙酸乙酯、正丁醇萃取,采用正相硅胶,C-18,Sephadex-20柱层析等方法,进行了分离纯化,共分离得到7个化合物。根据化学方法结合波谱分析数据(1D-NMR,2D-NMR)对分离所得化合物进行了结构鉴定,确定7个化合物分别是:迷迭香酚rosmanol(Ⅰ)、桦木酸(betulinicacid)(Ⅱ)、桦木醇betulin(Ⅲ)、7,24-tirucalladien-3β,27-diol(Ⅳ)、tirucalla-7,24-dien-3β,21,23-triol(Ⅴ)、迷迭香醌(rosmanol quinone)(Ⅵ)、芫花素(genkwanin)(Ⅶ),从迷迭香中分离得到的化合物Ⅳ、Ⅴ,鲜见文献报道。     

山橙的化学成分研究

综合运用硅胶柱层析、ODS柱层析、Sephadex LH-20凝胶柱层析以及制备高效液相等色谱分离技术,对夹竹桃科山橙属植物山橙(Melodinus suaveolens)枝叶的化学成分进行系统分离与纯化。结合理化性质和现代波谱技术,并通过与文献对照,从山橙枝叶90%乙醇提取物的石油醚萃取部位中分离得到10个化合物,分别鉴定为tabersonine(1)、scandine(2)、venalstonine(3)、venalstonidine(4)、pachysiphine(5)、scandine Nb-oxide(6)、科罗索酸(7)、乌发醇(8)、伞形花内酯(9)和6-羧基-伞形花内酯(10)。化合物7~10为首次从山橙中分离得到。     

灵芝三萜提取工艺研究

研究灵芝中三萜的提取工艺.方法:采用单因素试验法和正交试验法,以灵芝三萜的提取率为考察指标,考察溶剂浓度、溶剂用量、温度、时间等因素对提取率的影响.结果:浸提温度是影响三萜提取率的主要因素.最佳工艺条件:溶剂为无水乙醇,溶剂用量为18倍量,时间2 h,温度80℃,此条件下三萜提取率达94.47%.结论:该工艺的三萜提取率高,可用于灵芝中三萜的提取.     

柿叶三萜类化合物提取工艺条件研究

采用单因素实验和正交设计实验法对柿叶中三萜类化合物的提取工艺进行了研究。结果表明,以70%乙醇为溶剂的回流提取效果最佳;提取的最佳条件为料液比1：20,提取次数为4次,提取时间为2 h,三萜化合物的提取率可达2.16%。     

Microbial-Catalyzed Biotransformation of Multifunctional Triterpenoids Derived from Phytonutrients

Microbial-catalyzed biotransformations have considerable potential for the generation of an enormous variety of structurally diversified organic compounds, especially natural products with complex structures like triterpenoids. They offer efficient and economical ways to produce semi-synthetic analogues and novel lead molecules. Microorganisms such as bacteria and fungi could catalyze chemo-, regio- and stereospecific hydroxylations of diverse triterpenoid substrates that are extremely difficult to produce by chemical routes. During recent years, considerable research has been performed on the microbial transformation of bioactive triterpenoids, in order to obtain biologically active molecules with diverse structures features. This article reviews the microbial modifications of tetranortriterpenoids, tetracyclic triterpenoids and pentacyclic triterpenoids.     

New Triterpenoids with Cytotoxic Activity from Actinidia Valvata

Two new triterpenoids, 30-O-β-d-glucopyranosyloxy-2α,3α,24-trihydroxyurs-12, 18-diene-28-oic acid O-β-d-glucopyranosyl ester (1) and 2α,3β,3,30-tetrahydroxyurs-12, 18-diene-28-oic acid O-β-d-glucopyranosyl ester (2) were isolated from roots of Actinidia valvata Dunn. Their structures were elucidated by means of extensive spectroscopic studies. Both these two new compounds showed moderate cytotoxic activity in vitro against BEL-7402 and SMMC-7721 tumor cell line.     

Neurotrophic and Immunomodulatory Lanostane Triterpenoids from Wood-Inhabiting Basidiomycota

Neurotrophins such as nerve growth factor (ngf) and brain-derived neurotrophic factor (bdnf) play important roles in the central nervous system. They are potential therapeutic drugs for the treatment of neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease. In this study, we investigated the neurotrophic properties of triterpenes isolated from fruiting bodies of Laetiporus sulphureus and a mycelial culture of Antrodia sp. MUCL 56049. The structures of the isolated compounds were elucidated based on nuclear magnetic resonance (NMR) spectroscopy in combination with high-resolution electrospray mass spectrometry (HR-ESIMS). The secondary metabolites were tested for neurotrophin (ngf and bdnf) expression levels on human astrocytoma 1321N1 cells. Neurite outgrowth activity using rat pheochromocytoma (PC-12) cells was also determined. Twelve triterpenoids were isolated, of which several potently stimulated the expression of neurotrophic factors, namely, ngf (sulphurenic acid, 15α-dehydroxytrametenolic acid, fomefficinic acid D, and 16α-hydroxyeburicoic acid) and bdnf (sulphurenic acid and 15α-dehydroxytrametenolic acid), respectively. The triterpenes also potentiated ngf-induced neurite outgrowth in PC-12 cells. This is, to the best of our knowledge, the first report on the compound class of lanostanes in direct relation to bdnf and ngf enhancement. These compounds are widespread in medicinal mushrooms; hence, they appear promising as a starting point for the development of drugs and mycopharmaceuticals to combat neurodegenerative diseases. Interestingly, they do not show any pronounced cytotoxicity and may, therefore, be better suited for therapy than many other neurotrophic compounds that were previously reported.     

Triterpenoids from the Roots of Rhaphiolepis indica var. tashiroi and Their Anti-Inflammatory Activity

Two new triterpenoids, 2α,3β-dihydroxyolean-11,13(18)-dien-19β,28-olide (1) and 3β,5β-dihydroxyglutinol (2), together with eight known compounds (3–10) were isolated from the roots of Rhaphiolepis indica var. tashiroi (Rosaceae). The structures of 1–10 were determined by spectroscopic techniques. Among these isolates, 2α,3β-dihydroxyolean-13(18)-en-28-oic acid (9) exhibited inhibitory effect on N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide production, with an IC₅₀ value of 16.50 μM.     

粉煤灰釉面砖试验研究

以粉煤灰为主要原料，以其他硅酸盐原料为辅助原料，试制出性能优良的陶瓷釉面砖。笔者主要讨论了粉煤灰掺入量、烧成温度和气氛等工艺因素对釉面砖性能的影响，并确定了粉煤灰最佳掺入量和生产工艺。