Metmyoglobin Reducing Capacity of Fresh Normal, PSE, and DFD Pork During Retail Display

Fresh pale, soft, exudative (PSE), dark, firm, dry (DFD), and normal pork were stored under light or dark conditions at 4°C for 7 days. Sample pH, metmyoglobin reductase activity, oxygen consumption rate, and relative surface metmyoglobin and oxymyoglobin contents were determined. DFD pork had the highest metmyoglobin reductase activity and oxygen consumption rate. Enzyme activity of PSE was lower than that of normal pork, but no difference existed in oxygen consumption rate between PSE and normal samples. Metmyoglobin reductase activity dropped slowly during meat storage; oxygen consumption rate sharply decreased during the first day of storage. Both metmyoglobin reductase activity and oxygen consumption rate declined more rapidly in the light. Results can help develop guidelines for display and packaging of pork.     

Consumer-rated Quality Characteristics as Related to Purchase Intent of Fresh Pork

Consumers (n=556) rated pink color, wet/dry appearance, overall acceptability and purchase intent of normal, pale, soft and exudative (PSE), and dark, firm and dry (DFD) pork loin slices in PVC packages in five retail supermarkets. DFD samples appeared driest and PSE samples appeared wettest. Samples in the higher ranked two purchase intent categories appeared more wet than those in the lower three categories. The pinker PSE samples and all DFD samples were in the higher ranked purchase intent categories. Purchase intent generally paralleled overall acceptability; however, prediction equations indicated that wet/dry appearance, color and overall acceptability all contributed to purchase intent decision depending on muscle condition.     

Acceptability and Shelf-life of Marinated Fresh and Precooked Pork

Fresh (FL) and precooked (PL) longissimus chops and fresh semi-membranosus/adductor (SM) roasts were evaluated to determine acceptability of marinated pork. Citric acid and sodium acid pyrophosphate lowered pH (CAP), sodium acid pyrophosphate attained intermediate pH (SAP), and sodium tripolyphosphate increased pH (TPP). In FL and SM cuts, TPP improved sensory characteristics, water-holding capacity (WHC) and processing yields. CAP reduced microbial growth in FL chops and improved tenderness in SM roasts. In PL cuts, TPP improved juiciness and reheating yields. Marinating at low pH reduced microbial growth; marinating at high pH improved sensory properties, WHC, fresh pork processing yields and precooked pork juiciness and reheating yields.     

Konjac Flour Gel as Fat Substitute in Low-fat Prerigor Fresh Pork Sausage

Prerigor trimmings from four lean cuts of pork carcasses (n = 3) were used to manufacture three replications of low-fat (10%) fresh pork sausage containing konjac flour gel, at 0, 10 or 20% levels and compared to a 40% fat control. Treatment sausages showed equal or improved cooked yields, slightly higher shear force (kg/g) and sensory textural attributes, but rated slightly lower in juiciness. As konjac flour gel levels increased, shear force and sensory textural attributes became more like the control. Storage time had minimal effect on quality and shelf life. Acceptable low-fat, prerigor pork sausage can be produced with 10–20% incorporation of konjac flour gel.     

正常肉和白肌肉的蛋白质变化

测定白肌肉(Pale,Soft,Exudative,PSE)和正常猪肉(Reddish-pink,Firm,Non-exudative)持水性指标和在宰后不同时间的蛋白溶解性和蛋白降解程度。结果显示:PSE肉的各种蛋白溶解性都显著低于正常肉(p<0.05),宰后4 h的总蛋白、肌浆蛋白和肌原纤维蛋白的溶解性与滴水损失和贮藏损失都呈显著负相关,与持水力呈显著正相关。正常肉和PSE肉的肌浆蛋白和肌原纤维蛋白的SDS-PAGE电泳条带存在差异,PSE肉早期伴肌动蛋白(Nebulin)含量明显低于正常肉。可见PSE肉肌原纤维的降解较快,宰后早期的蛋白溶解性可以用于预测猪肉的持水性。     

气调包装(MAP)在冷却肉保鲜中的应用

相比热鲜肉、冷冻肉,冷却肉具有安全系数高,营养价值高,保质期长的特点,是目前国外广泛应用的生鲜肉类销售形式,气调包装应用于冷却肉保鲜,能大大延长肉的保质期,并能很好地保持肉的色泽,本文阐述了气调包装保鲜机理及气体组分的研究进展,论述了气调包装的工艺要点及其设备发展现状,分析并提出了气调包装应用于冷却肉保鲜的关键技术和应用前景。     

Physicochemical and Sensory Qualities of Fresh Pork Chops as Affected by Phosphate, Ascorbate or Sorbate Dipping Prior to Irradiation

Fresh pork chops were dipped for a target absorption of 0.5% sodium tripolyphosphate (STPP), 550 ppm sodium ascorbate (SA), or 0.1% potassium sorbate (PS) prior to irradiation (1.0 kGy). Untreated pork chops, both irradiated and unirradiated, were used as controls. Dipping with STPP decreased drip loss and improved color and lipid stability of irradiated chops and resulted in better tenderness and juiciness scores than undipped, irradiated samples. Also, STPP treated chops had similar or better physicochemical and sensory properties than untreated (no irradiation, no dipping) controls. Dipping with SA or PS had little effect compared with STPP, but improved some sensory qualities.     

生鲜冷却肉MAP包装保鲜技术的研究

应用MAP包装设备及技术研究生鲜冷却肉的保鲜,研究不同气氛条件下冷却肉的指标(感官性质、TVB-N、pH、菌落总数、货架期)。研究结果表明,CO2浓度为30%~40%,O2浓度为40%~60%时,对生鲜冷却肉有较好的保鲜效果,冷鲜肉MAP保鲜理想气氛条件为CO2%L∶O2%∶N2%=30∶50∶20,在4℃条件下储藏,货架期达8d以上。     

微波萃取-分光光度法测定新鲜猪肉中的含水量

利用氯醌酸与水显色这一原理,建立了分光光度法测定肉中含水量的方法。当乙醇溶液中含水量在0.04%～2%时,含水量与吸光度值呈正比关系,最大吸收波长为530 nm。文中采用了高压微波辅助萃取技术对样品进行预处理,操作简便、快速,测量结果与国标方法相比无显著性差异。方法的检出限为69μg/g,RSD(n =6)为0.029%。     

畜禽宰后肌肉能量代谢与肉品质研究进展

畜禽宰后肌肉通过糖酵解等能量代谢途径提供ATP满足能量需要,产物乳酸的生成会降低pH。糖酵解的速度和程度影响了pH的下降速度和程度,进而调控肉品色泽、嫩度、持水性、多汁性、风味等品质。本文综述了畜禽宰后肌肉能量代谢的基本路径,论述了腺苷酸激活蛋白激酶对糖酵解的调控机理及其对肉品质的影响,并着重叙述了白肌肉(pale soft and exudative meat,PSE)的预防措施,为在生产过程中肉品质调控提供思路。     

宰前处理和冷却方式对猪肉品质的影响

本文对宰前处理方式、致晕方法和冷却方式对猪肉品质(肉色、滴水损失/持水性-WHC和电导率)的影响进行了比较总结,旨在对屠宰行业在控制肉品质,减少PSE发生方面提供参考。     

Fatty Acid and Cholesterol Changes in Pork Longissimus Muscle and Fat due to Ractopamine

Fatty acid (FA) composition and cholesterol (CHL) content from control and Ractopamine (Rac) treated pigs were evaluated. Lipids were extracted from the longissimus (LD) and subcutaneous fat (SC) and methyl esters of the FA were formed for gas chromatography. CHL content of the LD was determined using direct saponification. Pigs were fed 0, 5, 10 or 20 ppm Rac for about 40 kg of grain prior to slaughter. Rac had no consistent effect on the FA composition of SC or muscle (neutral or polar) lipids. CHL content in the LD of treated animals was about 9% lower (P < 0.01) than controls. Rac reduced the CHL content of the LD and had limited effect on the FA profile of muscle or SC fat.     

Physical and Sensory Characteristics of Fresh Pork Leg Roasts Cooked at Low Temperatures

The effects of oven temperatures of 82, 93, 121 and 163°C were studied using 31 fresh pork leg roasts cooked to 74°C in oven bags. Warmed-over-flavor (WOF) was measured by sensory analysis after holding 3 hr at 68°C and after holding 24 hr at 2°C and then reheating to 68°C prior to serving. Roasts cooked at 82 and 93°C tended to have less WOF after refrigeration and reheating than roasts cooked at 121 and 163°C. Roasts cooked at 93°C had fewer aerobic mesophilic bacteria than those cooked at 121 and 163°C. In general, objective and sensory perception measurements indicated that roasts cooked at 82 and 93°C were of higher quality.     

Lipid Oxidation in Cooked Pork as Affected by Vitamin E, Cooking and Storage Conditions

The individual and combined effects of muscle vitamin E level, cooking conditions (duration, temperature and rate) and packaging on lipid oxidation in refrigerated cooked pork were examined. Oxidative stability following cooking was higher in pork with a higher vitamin E level (p<0.01), cooked at a lower cooking temperature (p<0.01), cooked for a shorter time (p<0.01), cooked at a faster cooking rate (p<0.05) or stored in vacuum packs (p<0.01). Significant two-way and three-way interactions were observed between the effects of muscle vitamin E level, cooking conditions and packaging on lipid oxidation. Adopting more than one of these approaches to minimize lipid oxidation was more effective than adopting a single approach.     

Low-dose Gamma Irradiation and Refrigerated FStorage in vacua Affect Microbial Flora of Fresh Pork

Vacuum-packaged ground fresh pork samples absorbed gamma radiation doses of 0. 0.57. 1.91. 3.76. 5.52. or 7.25 kGv at 2°C. Samolcs were analyzed after 1, 7, 14, 21, 28,‘or 35 days itorage at 2°C’for presence and number of aerobic and anaerobic mesophiles and endospore formers, and aerobic psychrotrophs. Conventional plate counts did not detect surviving microflora in any sample that received an absorbed dose of 1.91 kGy or higher, even after refrigerated storage for up to 35 days. The microflora in the control were predominantly Gram-positive for the first 21 days; however, Serratia predominated at 28 and 35 days. Staphylococcus, Micrococcus, and yeast species predominated in samples that received 0.57 kGy.     

Physical, Chemical and Sensory Changes in Irradiated Fresh Pork Packaged in Modified Atmosphere

The effects of irradiation dose (0, 0.5 and 1.0 kGy), headspace oxygen (0, 10 and 20% O₂, balance nitrogen) and storage temperature (5, 15 and 25°C) on the physical, chemical and sensory changes in fresh pork were studied using factorial design experiments. Irradiation in the absence of oxygen extended the sensory shelf life of pork from 9 to 26 days at 5°C and from & lt; 2 to 2 days at 25°C. Oxygen in the package headspace combined with irradiation adversely affected physical, chemical and sensory characteristics of the end product.     

Monoclonal Antibodies to Porcine Thermal-Stable Muscle Protein for Detection of Pork in Raw and Cooked Meats

Four hybridoma cell lines secreting monoclonal antibodies (MAbs) specific to porcine thermal-stable muscle proteins (TSMPs) were developed. The MAbs reacted with three protein bands (20.5, 22 and 24 kD) from raw pork extract; the protein band (24 kD) which was also present in cooked pork was identified as porcine-specific TSMP. Epitope analysis indicated four MAbs recognized same or closely located antigenic sites on the pork TSMP. The developed MAb-based indirect enzyme-linked immunosorbent assay (ELISA) enabled the detection of pork in raw and cooked heterogeneous meat mixtures as low as 10 g/kg. The curvilinear relations of second-degree polynomial (r² > 0.995) between pork concentrations and ELISA responses enabled quantifying degree of adulteration of pork in meat products.     

不同工艺条件对猪胴体和冷却猪肉微生物去污染效果的影响

调查了冷却猪肉生产企业现有生产工艺下微生物污染的状况 ;研究了对现有生产工序 (冲淋工序、冷却工序 )采用新的处理工艺 ,包括水冲洗、乳酸喷淋、冷分割以及联合处理等对猪胴体和冷却猪肉微生物去污染的效果。结果表明 ,在现有生产工艺下 ,冷却猪肉微生物污染随季节发生显著变化 ,且达不到HACCP体系微生物控制要求 ;水冲淋、乳酸喷淋、冷分割单独处理或联合处理 ,均有显著的微生物去污染效果。如果采用热分割 ,劈半后冲洗 1min ,乳酸喷淋 1min ,则微生物去污染效果显著 ,可基本达到HACCP对微生物控制要求。如果采用冷分割 ,劈半后冲洗 1min ,冷却 2 4h ,再次采用冷分割效果较好 ,冷却猪肉可基本达到HACCP对微生物控制要求 ;若劈半后冲洗 1min ,乳酸喷淋 1min ,冷却 2 4h则可完全达到HACCP对微生物控制要求。     

氧气体积分数对气调保鲜猪肉品质的影响

将从无锡当地市场购买的新鲜猪肉装入每盒分别含5％，15％，25％，35％，45％，55％的O2与20％的CO2的包装盒内，在4℃下储存8d。测定样品中的TVBN值，TBARs值，颜色分值，感官评定值和微生物总数，结果表明：猪肉气调保鲜时氧气的较好配比为45％和55％，然而，TBARs值在45％O2时比在55％O2时要低。因此，45％O2和20％CO2气体混合物可作为最适宜的组合气体来包装新鲜猪肉。     

基于紫外荧光成像对包装鲜猪肉存储时间预测研究

根据猪肉存储过程中脂质氧化荧光代谢产物随存储时间增加特点,开发了一套基于紫外荧光成像原理的包装鲜猪肉存储时间检测系统.选用肥瘦兼具的带皮五花肉,采集可见光彩色图像及紫外荧光图像.图像分割出肌肉区域、背膘及肉皮及荧光区域,进而提出背膘及肉皮部分中荧光区域所占图像的面积百分比作为新鲜度预测指标.研究结果显示该检测指标与存储...