THE EFFECT OF COATINGS AND PROCESSING METHODS ON DEHYDRATED PRECOOKED WHOLE PINTO BEANS QUALITY1

Splitting is a major problem in preparing dehydrated precooked beans. To reduce splitting and improve the product quality of precooked pinto beans, three coating treatments and two processing methods were investigated. Beans were blanched, soaked, steam precooked, coated by dipping in a biopolymer solution, and dehydrated in an air-circulating dryer. Coating beans in a 20% dextrin solution (Lo-Dex 5, M100, or K4484) reduced 43–85% of butterflying (severe splitting) of beans. Steam precooking at 121C/30 min with soaking at 22C/12 h yielded less butterflying than steam precooking at 100C/1 h with soaking at 30C/2 h and 82 C/1 h after coating and dehydration (?3% vs 8%). The former resulted in lower firmness after precooking and rehydration of dehydrated beans, whereas the latter gave beans a more mealy texture and better appearance. The rehydration time of dehydrated precooked beans in boiling water to produce an acceptable firmness was 6–9 min, depending on the processing methods. Coating resulted in darker color and slower rehydration of dehydrated beans.     

Development of impact acoustic detection and density separations methods for production of high quality processed beans

Impact acoustic detection (IAD) and density separation (DS) techniques were used to process and study the acoustic and physical properties of dry beans. The IAD’s data analysis software consisted of real time Fast Fourier Transform (FFT) function and the integral against frequency 0–20 kHz was called signal level (SL). Solutions of calcium nitrate and water were used for DS. Bean with low SL (LSL: SL ? 0.04) had higher percentage of skin damage and vice versa. Beans with high SL (HSL: SL > 0.04) were larger sized than those with LSL. Beans with low density had more skin damage than beans with high density. The quantity of broken dehydrated precooked pinto beans in LSL fraction was four times more than in HSL fraction. The percentages of burst and split beans in LSL fraction were higher than in HSL fraction. Higher solid losses and hydration ratio were associated with low density. Contrary to conventional methods, IAD could separate beans based on skin damage. Both techniques could improve high quality processing of dehydrated precooked beans.     

Quality Evaluation of Pinto Beans and Green Beans Canned in Two Large Reusable Containers

ABSTRACT: As an alternative to standard metal cans, 2 large-scale, reusable containers were constructed with volumetric capacities 3.6 to 3.8 times greater and process times 1.36 to 2.05 times longer than a nr 10 can. Effects of increased process time on product quality were measured by conducting objective color and texture evaluations on green beans and pinto beans canned in both containers. Green beans and pinto beans had comparable texture profiles to beans processed conventionally in nr 10 cans. The color of green beans also was comparable; the color of pinto beans was darker, possibly due to contact with the metal container. Color and texture differences were minimal between inner and outer product layers.     

DRY BEAN (PHASEOLUS VULGARJS) COLOR STABILITY AS INFLUENCED BY TIME and MOISTIJRE CONTENT

The color stability of 32 lines of beans to which different levels of moisture were added was evaluated in an accelerated shelf-life testing chamber for up to 10 weeks using Hunter values and National Bureau of Standard (NBS) unit of color difference (± E) techniques. With pinto beans, Hunter L values decreased as storage time increased. Also, Hunter a values increased with time. However, Hunter b values did not change with storage. With great northern beans, Hunter a and b values were more important than Hunter L value. Similar to pinto beans, along with prolonged storage, great northern bean color changed. Pinto and great northern beans having an extra 10% added moisture showed much more color change than those of control or 5% moisture added beans. Finally, bean genotype was closely related with color stability in stored pinto and great northern beans.     

Feed Moisture Effects on Functional Properties, Trypsin Inhibitor and Hemagglutinating Activities of Extruded Bean High Starch Fractions

High starch fractions (HSF) of navy, pinto and garbanzo beans were extruded with different moisture contents using a Wenger X-5 extruder. Increasing moisture content resulted in increased expansion index for navy and garbanzo beans and a decrease for pinto bean. The highest water absorption index was obtained for navy bean HSF at moisture 27%, for pinto 30% and for garbanzo 26%. For all beans, water solubility index decreased with increasing moisture. Color of extrudates also was affected by moisture. The extrusion process reduced trypsin inhibitor activity by about 70–85%, the level of decrease determined by moisture content of starting material and temperature. Extrusion temperatures of 121, 132 and 150°C inactivated hemagglutinating activity of navy, pinto and garbanzo beans.     

Canning Quality Evaluation of Pinto and Navy Beans

Thermal processing of pinto and navy beans at 121.1°C for 16 or 14 min in a still retort gave similar sterilization value (F_o= 10) as the processing at 115.6°C for 45 min. The 121.1°C/16 or 14 min process produced beans with greater firmness than the 115.6°C/45 min process. The addition of CaCl₂ and EDTA improved firmness and color of canned beans. Calcium chloride also reduced clumping and splitting of the canned beans. Sensory evaluation showed that the acceptability of canned beans was reduced when CaCl₂ was increased up to 10 mM. High correlation between firmness and soluble pectin in various bean cultivars implied that soluble pectin content could be used as a parameter for screening bean cultivars with desirable firmness.     

Changes in Selected Biochemical Components, In Vitro Protein Digestibility and Amino Acids in Two Bean Cultivars During Germination

This study investigates changes in lectin and trypsin inhibitor activity, SDS-PAGE peptide pattern, in vitro protein digestibility and amino acid composition during germination of two dry bean cultivars. Lectin activity in navy beans was reduced. Significant amounts of trypsin inhibitor activity in both navy and pinto beans remained after germination for 6 days. Glycopeptides, with molecular mass ranging from 25,000 to 27,000 daltons, from partial proteolysis of the major storage proteins were resistant to a multienzyme system. In vitro protein digestibility and amino acid composition were only slightly altered. Germination did not improve protein nutritional quality of dry beans.     

Chlortetracycline detoxification in maize via induction of glutathione S-transferases after antibiotic exposure

Soil contamination with nonmetabolized antibiotics is an emerging environmental concern, especially on agricultural croplands that receive animal manure as fertilizer. In this study, phytotoxicity of chlortetracycline (CTC) antibiotics on pinto beans (Phaseolus vulgaris) and maize (Zea mays) was investigated under controlled conditions. When grown in CTC-treated soil, a significant increase in the activities of the plant stress proteins glutathione S-transferases (GST) and peroxidases (POX) were observed in maize plants, but not in pinto beans. In vitro conjugation reactions demonstrated that the induced GST in maize catalyzed the conjugation of glutathione (GSH) with CTC, producing stable conjugates that were structurally characterized using liquid chromatography/mass spectrometry. The antibiotic-induced GST produced CTC-glutathione conjugate at relative concentrations 2-fold higher than that produced by constitutively expressed GST extracted from untreated maize. On the other hand, GST extracted from pinto beans (both treated and untreated) did not efficiently catalyze glutathione conjugation with CTC. These results suggest that maize is able to detoxify chlortetracycline via the glutathione pathway, whereas pinto beans cannot. This may explain the observed stunted growth of pinto beans after antibiotic treatment. This study demonstrates the importance of plant uptake in determining the fate of antibiotics in soil and their potential phytotoxicity to susceptible plants.     

Effect of Germination on Oligosaccharides and Nonstarch Polysaccharides in Navy and Pinto Beans

Effect of germination on raffinose-oligosaccharides, and nonstarch polysaccharides in C-20 navy and Pindak pinto beans was investigated using solvent fractionation and chromatographic methods. Approximately 70-80% of total raffinose and stachyose was removed by germination for 6 days. Ungerminated navy and pinto beans contained 21.75% and 18.84% total nonstarch polysaccharides, respectively. Germination did not significantly change the yields of soluble and insoluble nonstarch polysaccharides. Arabinose in the soluble nonstarch polysaccharides was significantly increased by germination. Germination increased the viscosity of soluble nonstarch polysaccharides. However, the viscosity increase of the soluble nonstarch polysaccharides from germinated pinto beans was significantly greater than that from germinated navy beans.     

Nutrient Composition, Protein Quality, and Sensory Properties of Thirty-Six Cultivars of Dry Beans (Phaseolus vulgaris L.)

Thirty-six cultivars representing eight types of dry beans (Phaseolus vulgaris L) were analyzed chemically for content of moisture, fat, ash, total nitrogen, iron, zinc, calcium, magnesium, potassium, phosphorus, thiamin, riboflavin, and amino acids. Protein quality was evaluated using Tetrahymena pyriformis W. Cooked beans were subjected to sensory evaluation and shear-force measurement. Nutrient concentrations were similar to values reported by earlier investigators. Protein quality ranged from 60% (Red Kidneys) to 90% (Pintos) that of casein. Pintos Nodak and Fiesta and Hyden Navy beans were most acceptable in flavor and texture; Bonus Small White was least acceptable. Cooperation between bean breeders and nutritionists will enable early screening of segregating hybrid populations for desirable nutritional and sensory qualities.     

Differentiating the Lectin Activity in Twenty-Four Cultivars of Dry Beans (Phaseolus vulgaris L.)

Hemagglutination activity of 24 cultivars of dry beans (Phaseolus vulgaris) was estimated with rabbit, rat, bovine, and human erythrocytes. Net protein utilization (NPU) evaluated the nutritional toxicity to weanling rats of the beans fed at 10% protein. Nonprotein and 10%-casein control diets were included. SDS-polyacrylamide gel electrophoresis indicated lectin-containing beans. Beans evaluated (in or-der of decreasing lectin activity) were: Aurora, Sanilac, Royal Red, Red Kloud, Roza, Rufus, Harris, Viva, Fiesta, Black Turtle Soup, Chief, Hyden, UI-59, Sutter; (nontoxic): Blue Mountain, GN-1140, Holberg, Nodak, Olathe, Pindak, JM-126, NW-410, NW-590, UI-114. This study shows that combined hemagglutination tests and SDSPAGE enable rapid screening of dry beans for toxic lectins.     

Reduction of antiproliferative capacities,cell-based antioxidant capacities and phytochemical contents of common beans and soybeans upon thermal processing

The effects of boiling and steaming processes on the antiproliferative and cellular antioxidant properties, as well as phytochemicals, of two types of common beans (pinto and black beans) and two types of soybeans (yellow and black) were investigated. All thermal-processing methods caused significant (p < 0.05) decreases in total phenolic content (TPC), total saponin content (TSC) and phytic acid content (PAC) values in all bean types (except for TPC values in pressure-steamed yellow soybeans) as compared to those of the raw beans. All types of uncooked raw beans exhibited cellular antioxidant activities (CAA) in dose-dependent manners. Black soybeans exhibited the greatest CAA, followed by black beans, pinto beans and yellow soybeans. The CAA of cooked beans were generally diminished or eliminated by thermal processing. The hydrophilic extracts from raw pinto beans, black beans and black soybeans exhibited antiproliferation capacities against human gastric (AGS) and colorectal (SW480) cancer cells in dose-dependent manners. The raw yellow soybeans exhibited dose-dependent antiproliferation activities against the SW480 cells. Most of the cooked beans lost their antiproliferation capacities as observed in the raw beans. These results indicate that different processing methods may have various effects on phytochemical profiles and bioactivities. Overall, thermal processing caused a significant reduction of the health-promotion effects of beans.     

Effect of Infrared Heat Processing on Rehydration Rate and Cooking of Phaseolus vulgaris (Var. Pinto)

Imbibition studies and cooking tests were conducted to evaluate the effect of infrared (IR)-heating on pinto beans (Phaseolus vulgaris) heated to 99°C and 107°C. IR-heating improved rehydration rate and increased degree of swelling of Pinto beans. Water absorption capacities and imbibition rates were higher for the 99°C than 107°C samples. However, tests showed IR-heating significantly increased cooking time of pinto beans.     

Impact of Commercial Precooking of Common Bean (Phaseolus vulgaris) on the Generation of Peptides,After Pepsin–Pancreatin Hydrolysis,Capable to Inhibit Dipeptidyl Peptidase‐IV

The objective of this research was to determine the bioactive properties of the released peptides from commercially available precook common beans (Phaseolus vulgaris). Bioactive properties and peptide profiles were evaluated in protein hydrolysates of raw and commercially precooked common beans. Five varieties (Black, Pinto, Red, Navy, and Great Northern) were selected for protein extraction, protein and peptide molecular mass profiles, and peptide sequences. Potential bioactivities of hydrolysates, including antioxidant capacity and inhibition of α‐amylase, α‐glucosidase, dipeptidyl peptidase‐IV (DPP‐IV), and angiotensin converting enzyme I (ACE) were analyzed after digestion with pepsin/pancreatin. Hydrolysates from Navy beans were the most potent inhibitors of DPP‐IV with no statistical differences between precooked and raw (IC₅₀ = 0.093 and 0.095 mg protein/mL, respectively). α‐Amylase inhibition was higher for raw Red, Navy and Great Northern beans (36%, 31%, 27% relative to acarbose (rel ac)/mg protein, respectively). α‐Glucosidase inhibition among all bean hydrolysates did not show significant differences; however, inhibition values were above 40% rel ac/mg protein. IC₅₀ values for ACE were not significantly different among all bean hydrolysates (range 0.20 to 0.34 mg protein/mL), except for Red bean that presented higher IC₅₀ values. Peptide molecular mass profile ranged from 500 to 3000 Da. A total of 11 and 17 biologically active peptide sequences were identified in raw and precooked beans, respectively. Peptide sequences YAGGS and YAAGS from raw Great Northern and precooked Pinto showed similar amino acid sequences and same potential ACE inhibition activity. Processing did not affect the bioactive properties of released peptides from precooked beans. Commercially precooked beans could contribute to the intake of bioactive peptides and promote health.     

Effect of Temperature on Properties of Extrudates from High Starch Fractions of Navy, Pinto and Garbanzo Beans

High starch fraction (HSF) isolated from three legumes were extruded at different temperatures. Extruded products were evaluated for expansion, density, color and some functional properties. The highest value of expansion was noted for pinto bean, and the lowest for garbanzo, extruded at 132°C. Extrudates from HSF expanded significantly (P < 0.05) less than corn extrudates. Oil absorption capacity increased slightly with increasing temperature and values were lower for navy and garbanzo beans than for pinto HSF and corn. Oil emul-sification capacity of- bean samples was about two times higher compared to corn. Water absorption index (WAI) of pinto and navy beans significantly increased (P < 0.05) from 110°C to 132°C.     

High Temperature Extrusion Effects on Protein Solubility and Distribution in Navy and Pinto Beans

Osborne protein solubility fractionation and polyacrylamide gel electrophoresis (PAGE) showed changes in protein distribution during high temperature extrusion of pinto and navy bean high starch fraction (HSF). A high degree of protein insolubility was found after extrusion, which resulted in a decrease in solubility of albumin and globulin fractions and an increase in the residue. An extrusion temperature of 110°C had a greater effect on solubility of albumin and globulin fractions of pinto than navy bean. Sodium dodecyl sulfate (SDS)-PAGE showed more changes in subunit patterns of albumin and globulin for pinto than for navy beans at 110°C. Higher temperatures of 135 and 150°C caused greater changes in gel electrophoretic patterns of albumin and globulin fractions of navy beans.     

Absorbability of Calcium From Common Beans

Absorption of calcium from white, red, and pinto beans, labeled with ⁴⁵Ca, was measured in 24 women in a three-way randomized design using a calcium load of 72 mg and milk as the referent. Fractional absorption for the three beans averaged 0.219 ± 0.047 and did not differ by type. Milk calcium absorption at the same load was more than two times higher, 0.451 ± 0.088 (P < 0.001). Oxalate content averaged 0.34%, and phytate averaged 1.7%, a stoichiometric excess relative to calcium. To evaluate the relation of phytate to reduced absorbability, labeled pinto beans were pre-treated with phytase and fed to 10 subjects. Fractional absorption rose, averaging 0.318 ± 0.071, (P <0.01 vs. untreated beans), but was significantly below that of milk. The difference was partly accounted for by phytate, with the remainder probably due to relatively high oxalate.     

Nutritional evaluation and bioactive microconstituents (phytosterols,tocopherols, polyphenols,triterpenic acids) in cooked dry legumes usually consumed in the Mediterranean countries

In the present study we report the crude composition and several bioactive microconstituents content in cooked dry legumes, usually consumed in Mediterranean countries. The legumes studied were broad beans, chickpeas, two split peas varieties, two lentils varieties, pinto beans, black-eyed beans, five white beans varieties and white lupines.     

Incorporation of pulse flours of different particle size in relation to pita bread quality

BACKGROUND: To increase pulse consumption, pita bread was fortified with pulse flours milled from green lentils, navy beans and pinto beans, which were ground to produce fine and coarse flours. Pita breads were prepared using composite flours containing pulse flours (25, 50, 75%) and wheat flour or 100% pulse flours and adjusting the amount of water required for mixing based on farinograph water absorption. Pita bread quality was evaluated according to diameter, pocket height, specific loaf volume, texture and crust colour. RESULTS: Blends made from pulse flours with coarse particle size showed higher rates of water absorption. All composite flours and 100% pulse flours produced pitas with pockets, confirming their suitability for this product. Crust colour of pitas was affected less by navy bean flour than by lentil flour. Pita breads made with pinto bean flour were superior in texture. Overall, navy and pinto bean flours appeared more suitable for pita bread. Flours with coarse particle sizes produced pitas with better colour and texture. Sensory parameters of pitas containing 25% coarse pinto or navy bean flour were as good as or better than those from the wheat control. CONCLUSION: Acceptable pita breads can be made using pulse flours, although the substitution level is limited to 25%. Copyright © 2012 Society of Chemical Industry     

Grape cultivar and drying method affect sensory characteristics and consumer preference of raisins

The sensory attributes and consumer preference of raisins made from two grapevine cultivars, ‘Fiesta’ and ‘Selma Pete’, and two drying methods, dry‐on‐vine (DOV) or tray‐dry, were evaluated. Grapevine cultivar and drying method interacted to affect most of the measured flavor, texture, and appearance attributes of raisins. In general, ‘Fiesta’ DOV raisins were less spicy, less sour, more moist, less sticky and chewy, and less brown‐colored than other raisins. ‘Fiesta’ tray dried raisins were less sweet, more sticky and chewy, and less homogenous in color and in size. ‘Selma Pete’ raisins were more sour tasting and dark colored than ‘Fiesta’ raisins, especially the ‘Selma Pete’ DOV raisins. The average consumer hedonic test scores were not affected by cultivar or drying method, but further analyses identified clusters of people that clearly preferred raisins of different cultivars and drying methods. These findings suggest that raisins could be marketed on the basis of cultivar and drying method. Copyright © 2007 Society of Chemical Industry