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1.
《Food chemistry》1999,65(2):207-211
Selenium deficiency was produced in rats fed a high cholesterol diet for 57 days (Group 1). It was characterized by an increase in malondialdehyde (MDA) an end product of lipid peroxidation and by the dramatic collapse of selenium-dependent glutathione peroxidase activity (GSHPx) in plasma, erythrocytes and in homogenate supernatant fraction of liver, kidney and heart compared with rats fed a standard diet containing sodium selenite (Group 3). A compensatory rise in the activity of liver glutathione S-transferase (GST) activity and also in glutathione reductase (GSSGR) activity was accompanied by an increase in NADPH-generating enzymes glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. Adequate dietary selenium supplementation by Se-rich Spirulina corrected all the selenium deficiency effects (Group 2), then, GSHPx and NADPH-consuming enzymes activities were of the same magnitude as those exhibited by rats fed a standard diet containing adequate selenium in the form of sodium selenite. Based on this study, it is concluded that Se-enriched Spirulina behave as an excellent selenium carrier.  相似文献   

2.
An influense was studied in rats of selenium enriched phycocyanin (Se-PC) from food microalgae Spirulina on anaphylactic reaction severity and circulating antibody response against model allergen--hen's egg white ovalbumin. Se-PC was introduced into diet in form of protein isolate precipitated with ammonia sulphate. Se-PC dosage made up to 450 mcg per rat daily that corresponded to 5 mcg of selenium. There were no differences revealed between experimental and control group that received standard diet in severity of anaphylactic reaction. Nevertheless rats receiving Se-PC demonstrated significantly increased specific IgG response. The probable immunomodulating properties of Se-PC included into food are discussed.  相似文献   

3.
Rats were fed for two weeks low-selenium semi-synthetic diet supplemented with different sources of selenium: sodium selenite (selenium concentration 96 and 350 mcg/kg diet), selenium-enriched Spirulina (selenium concentration 350 mcg/kg diet) and selenium-enriched Phycocyanin (selenium concentration 96 mcg/kg diet). Selenium bioavailability was evaluated in terms of selenium accumulation in blood and liver as well as glutathione peroxidase activity. It was shown that selenium from selenium-enriched Spirulina and selenium-enriched phycocyanin was high bioavailable in rats.  相似文献   

4.
以雌性SD大鼠为研究对象建立低硒的动物模型,通过灌胃的形式给大鼠分别补充不同剂量的亚硒酸钠、富硒酵母和四棱豆硒蛋白。检测大鼠血清和肝肾中的硒含量、谷胱甘肽过氧化氢酶(GSH-px)、超氧化物歧化酶(SOD)、硫氧还蛋白还原酶(TrxR)的活性和丙二醛(MDA)的含量来比较其生物利用的效果。结果表明:与低硒对照组相比,所有加硒处理组的大鼠血、肝、肾中的硒含量以及所含的硒酶活性均明显提高(P<0.05),在同一补硒水平上,亚硒酸钠组的肝肾硒含量低于富硒酵母组和四棱豆硒蛋白组(P<0.05);亚硒酸钠组的血清和肝肾的谷胱甘肽过氧化氢酶(GSH-px)、超氧化物歧化酶(SOD)、硫氧还蛋白还原酶(TrxR)的活性和丙二醛(MDA)的含量都低于富硒酵母组和四棱豆硒蛋白组(P<0.05)。结论:四棱豆硒蛋白和富硒酵母增加大鼠的硒含量高于亚硒酸钠组(P<0.05),其它硒酶的影响是四棱豆硒蛋白最优,硒蛋白能更好地被低硒大鼠吸收利用。   相似文献   

5.
李茜  黄伟  黄蓓 《食品科学》2016,37(1):194-197
通过在Zarrouk培养基中添加亚硒酸钠的方法获得了硒含量达758.6 μg/g的富硒螺旋藻。采用量子共振技术检测富硒螺旋藻的主要氨基酸、维生素及抗癌能力、免疫功能、抗高脂血症相关功能性指标的量价值,发现富硒螺旋藻以上各项指标的量价值均高于普通螺旋藻。同时考察富硒螺旋藻对急性高脂血症小鼠血脂水平的影响,给予小鼠连续2 周灌胃富硒螺旋藻,通过尾静脉单次注射400 mg/kg的Triton WR-1339建立急性高脂血症小鼠模型,18 h后检测小鼠血清中血脂水平。结果表明:与Triton WR-1339对照组相比较,富硒螺旋藻高剂量组小鼠的血清中甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoproteincholesterol,LDL-C)含量明显降低(P<0.01),高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)含量明显升高(P<0.01)。与普通螺旋藻高剂量组相比,富硒螺旋藻高剂量组小鼠的血清中TG、LDL-C含量显著降低(P<0.05),HDL-C含量显著上升(P<0.05)。富硒螺旋藻对急性高脂血症有良好的保健功效。  相似文献   

6.
In order to increase food selenium (Se) content, Se‐enriched tea was produced by fertilising with sodium selenite in low‐Se soil. Five groups of rats were fed a low‐Se diet supplemented with either water (Se‐deficient), sodium selenite or an aqueous extraction of low‐Se tea, Se‐enriched tea or naturally high‐Se tea. The chemical form of Se in Se‐enriched tea and the physiological function in rats fed the different Se sources were determined after 8 weeks. The results showed that organic Se accounted for 80% or more of the Se in Se‐enriched tea fertilised with sodium selenite and naturally high‐Se tea, but no significant differences in the proportion of organic Se and protein Se were found between Se‐enriched tea and naturally high‐Se tea. The Se biological utilisation rates were 65.41, 68.05 and 70.49% for sodium selenite, Se‐enriched tea and naturally high‐Se tea respectively. The Se content of blood and liver and the glutathione peroxidase (GSH‐Px) activity were significantly increased by feeding Se‐enriched tea and sodium selenite compared with low‐Se tea, but a more efficient increase in liver GSH‐Px activity was obtained with Se‐enriched tea than with sodium selenite. No significant differences were found between Se‐enriched tea fertilised with sodium selenite and naturally high‐Se tea, which proved that the biological effectiveness of Se in Se‐enriched tea was higher than that of sodium selenite in increasing liver GSH‐Px activity. Se‐enriched tea fertilised with sodium selenite in low‐Se soil gave the same biological function as naturally high‐Se tea. Therefore Se‐enriched tea is a safe and effective means of increasing the Se intake of both humans and animals in low‐Se areas. © 2000 Society of Chemical Industry  相似文献   

7.
目的:观察肉鸡活体强化亚硒酸钠后,硒元素在鸡体内主要脏器中的形态及其含量,为富硒肉鸡的生产提供基础数据。方法:将6只雄性三黄鸡随机分为对照组、低剂量组和高剂量组,通过饮水途径自然摄取硒补充剂,亚硒酸钠浓度分别为0 mg/Kg.b.w.、2 mg/Kg.b.w.和10 mg/Kg.b.w.。实验结束后采用颈部动静脉放血方式处死实验动物,并采集肌肉、肝脏、肾脏、心脏、胃、血液等组织器官进行硒含量测定。结果:各组织脏器内的平均总硒含量分别为13.5~39.1μg/100g(对照组),68.8~365.8μg/100g(低剂量组)和109.2~809.7μg/100g之间(高剂量组),以肝脏和肾脏含量最高,且有机硒比例均在93%以上。结论:通过体内-短期-大剂量的活体强化,硒元素能以有机硒的形态在肉鸡体内各组织脏器富集,得到富含有机硒的肉鸡。  相似文献   

8.
垂盆草护肝胶囊的开发与研究   总被引:1,自引:1,他引:1  
陈永壮  王茵  傅逸根 《食品科学》2002,23(9):123-126
本品以垂盆草、螺旋藻为原料开发的保健食品垂盆草护肝胶囊,制定了相关质量标准,并对其进行了安全性评价及功能学评价,试验结果表明:(1)胶囊急性毒性属无毒类,无致突变作用,30d喂养结果未见明显毒性反应,无作用剂量为5.0g/kg体重。(2)垂盆草护肝胶囊对小鼠肝损伤的保护作用:在CCl4急性肝损伤建模后,低、中剂量组血清丙氨酸氨基转移酶活性均显著低于CCl4对照组(p>0.05),各剂量组肝脏病理损伤均较CCl4对照组明显减轻。  相似文献   

9.
Ripoll G  Joy M  Muñoz F 《Meat science》2011,87(1):88-93
The aim of this work was to determine the increase in the shelf life of modified atmosphere packaged fresh lamb meat due to the effect of dietary vitamin E and selenium supplementation on colour and lipid oxidation. 128 lambs were fed on a concentrate with standard levels of vitamin E (C), a concentrate enriched with vitamin E (V), a concentrate with sodium selenite (S) and a concentrate enriched with both vitamin E and sodium selenite (VS). The lambs were slaughtered at 27.3±1.45 kg LW, and chops stored on MAP for 7, 9, 11 and 13 days. CIELab colour and TBARs were studied on these days. Use of dietary vitamin E extended the shelf life a further 4 days from the commercial sell-by date in terms of lightness, hue angle, metmyoglobin formation and lipid oxidation. Selenium could be used to increase the lightness of meat without vitamin E supplementation in lambs' diets.  相似文献   

10.
以钝顶螺旋藻为研究对象,通过在培养液中分别添加钙铁锌硒离子并结合调节螺旋藻细胞的生长环境条件以达到以上四种营养元素最大积累的目的,并对其在细胞内的有机化程度进行研究。结果表明:与对照组相比,当温度为20 ℃、光照12 kLux、pH8.5时,钙积累量提高了97%,螺旋藻钙总含量和其有机化程度分别达到18.21 mg/g、84.17%;当温度为30 ℃、光照4 kLux、pH10.5时,铁积累量提高了175%,螺旋藻铁总含量和其有机化程度分别达到1634.34 ppm、89.98%;当温度为30 ℃、光照8 kLux、pH8.5时,锌积累量提高了87%,螺旋藻最高锌含量和有机化程度分别达到88.30 ppm、58.28%;当温度为30 ℃、光照12 kLux、pH9.5时,螺旋藻中硒元素总含量及有机化程度分别为1011.00 ppm、70.54%。因此,在以环境调控为基础,同时配合增加外源培养液浓度的培养条件下,能够使得螺旋藻钙、铁、锌、硒四种矿物质营养离子积累量大大增加。  相似文献   

11.
The vitamin security and selenium status were measured in the patients with unspecific ulcerative colitis. There were used food microalgae Spirulina platensis and it's preparation enriched with selenium as auxiliary tools of dietetic treatment for these patients. It's shown that there is a combined deficiency of beta-carotene and selenium and occasionally some other micronutrients in a significant part of the patients. The doses used of said food supplements were not enough sufficient for a dietary correction of deficiency of micronutrients with antioxidative properties.  相似文献   

12.
Spirulina platensis is a microalgae with potent dietary phyto-antioxidant, anti-inflammatory and anti-carcinogenic properties. We investigated the mechanism of cisplatin induced hepatotoxicity and whether this natural antioxidant provided protection against cisplatin hepatotoxicity. The study was carried out in a mice model where the animals were segregated into different groups according to their treatments, e.g. control group with no treatment, cisplatin treated, cisplatin + Spirulina treated, cisplatin + vitamin C treated and cisplatin + Spirulina + vitamin C treated. The liver marker enzymes were found to be elevated following cisplatin treatment, signifying hepatotoxicity. The supplementation of Spirulina and vitamin C could effectively bring down the levels of these enzymes. Light microscopy also showed that cisplatin treatment induced liver injury and that histopathological abnormalities were prevented by Spirulina and vitamin C supplementation. This protective effect was further substantiated by the estimation of antioxidant levels and extent of lipid peroxidation in the Spirulina, vitamin C and Spirulina + vitamin C supplemented groups as compared to cisplatin alone.  相似文献   

13.
常静瑶  庞广昌  李杨 《食品科学》2010,31(17):281-285
目的:分析螺旋藻多糖3 种不同给药途径对小鼠血清中24 种细胞因子的影响,进而研究螺旋藻多糖对小鼠的免疫调节作用。方法:通过灌胃、腹腔注射、尾静脉注射3 种途径分别给小鼠不同剂量的螺旋藻多糖,对照组给与实验组同等体积的生理盐水。灌胃组在3h 后眼球取血,腹腔注射和尾静脉注射组在2h 后眼球取血,分离血清,采用Millipore 小鼠细胞因子试剂盒和液体芯片扫描仪检测血清中24 种细胞因子的浓度。结果:3 种给药方式小鼠的IL-1α、IL-1β、IL-6、TNF-α、IFN-γ等炎症细胞因子都有不同程度的显著性升高,说明螺旋藻多糖对炎症细胞因子的分泌和先天免疫有促进作用。其中,腹腔注射螺旋藻多糖对细胞因子的影响最为显著,这种方式的信号传导途径主要包括NF- κB、MAPK、JAK/STAT 等。结论:从螺旋藻多糖对小鼠细胞因子的影响来看,总体上表现出促进免疫的作用,推测螺旋藻多糖主要是通过对肠黏膜系统的受体相互作用,刺激相应细胞产生细胞因子来发挥其免疫调节作用和多种生理功能的。  相似文献   

14.
Distribution of some trace elements elements (zinc, selenium, iron, manganese, chromium) was characterized in enriched biomass of food micro algae Spirulina platensis by means of water-methanol fractionation. The majority of said trace elements was shown to be incorporated in intercellular hydrophylic fraction, e.g. could be connected to cellular proteins. This result enable the conclusion, that Spirulina is a suitable matrix for biotechnological incorporation of new food trace elements preparations.  相似文献   

15.
Nuts from Bertholletia excelsa, commonly known as Brazil nuts, were analyzed for selenium. Of 529 nuts from one commercial source, 6% were found to contain 100 ppm selenium or more. The mean value for all nuts was 29.6 ppm and the median value was 13.4 ppm. Hexane extracted high-selenium Brazil nut meal in a corn-based diet fed to rats produced toxicity similar to that obtained from seleniferous corn, selenomethionine or sodium selenite as assessed by weight gain, visually scored liver damage, and liver, kidney, and spleen weights. Selenium contents of liver, kidney, spleen, and blood were also determined. It is suggested that the selenium in Brazil nuts is as biologically potent as that from other sources.  相似文献   

16.
钝顶螺旋藻的硒化培养研究   总被引:2,自引:0,他引:2  
在培养基中加入亚硒酸钠,对钝顶螺旋藻进行瓶式培养,研究不同加硒浓度、加硒方式对螺旋藻的生长状况及硒积累的影响。结果表明:钝顶螺旋藻中累积的硒随外加硒浓度的增加而增大,但累积系数与浓度的增加成负相关。分次加硒有利于螺旋藻的生长且较一次性加硒获得藻粉的含硒量更高。  相似文献   

17.
Selenium concentrations in blood serum of heifer calves from dams fed 0, 1, or 5 mg supplemental selenium daily during the dry period were compared. Dry cows were fed corn silage top dressed with a soybean meal mineral mix containing the desired amount of selenium. At birth, calves were assigned to one of six treatments in a 3 x 2 factorial arrangement. Calf treatments were 0, 1 (at birth), or 2 (at birth and 14 days of age) injections of selenium/vitamin E with feeding of starter commencing at 14 or 28 days of age. Dosage per injection was .078 mg selenium and 5.4 IU vitamin E per kilogram body weight. Selenium concentrations of blood serum of cows at parturition were 14, 32, and 58 ppb for cows fed 0, 1, and 5 mg selenium per day. Amount of selenium fed to cows affected selenium concentrations in blood serum of their calves with those from cows fed 5 mg selenium having the greatest concentrations. Calves from cows fed no supplemental selenium showed increased selenium in serum at day 28 and 42 when given one or two injections of selenium. Two injections, however, were necessary to elicit a response in calves from cows fed 1 mg per day. Selenium in blood serum of calves from cows fed 5 mg per day was not elevated when calves were given one or two injections of selenium. Prepartum selenium supplementation of the dam elevated selenium of blood serum in the calf at birth.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

18.
The objectives were to determine effects of graded levels of selenized yeast derived from a specific strain of Saccharomyces cerevisiae (CNCM I-3060) on animal performance and in selenium concentrations in the blood, milk, feces, and urine of dairy cows compared with sodium selenite; and to provide preliminary data on the proportion of selenium as selenomethionine in the milk and blood. Twenty Holstein cows were used in a 5 × 5 Latin square design study in which all cows received the same total mixed rations, which varied only in source or concentration of dietary selenium. There were 5 experimental treatments. Total dietary selenium of treatment 1, which received no added selenium, was 0.15 mg/kg of dry matter, whereas values for treatments 2, 3, and 4, derived from selenized yeast, were 0.27, 0.33, and 0.40 mg/kg of dry matter, respectively. Treatment 5 contained 0.25 mg of selenium obtained from sodium selenite/kg of dry matter. There were no significant treatment effects on animal performance, and blood chemistry and hematology showed few treatment effects. Regression analysis noted significant positive linear effects of increasing dietary selenium derived from selenized yeast on selenium concentrations in the milk, blood, urine, and feces. In addition, milk selenium results indicated improved bioavailability of selenium from selenized yeast, compared with sodium selenite. Preliminary analyses showed that compared with sodium selenite, the use of selenized yeast increased the concentration of selenomethionine in the milk and blood. There was no indication of adverse effects on cow health associated with the use of selenized yeast.  相似文献   

19.
Spirulina microalgae (Spirulina platensis, Spirulina maxima, Spirulina fusiformis) is considered as a valuable additional food source of some macro- and micronutrients including high quality protein, iron, gamma-linolenic fatty acid, carotenoids, vitamins B1 and B2. In spite of Spirulina chemical composition varies widely when grown in open reservoirs its efficiency as an additional remedy in treatment and prophylaxis of different diseases is proved in a variety of experimental and clinical trials. During Spirulina cultivation in open reservoirs and especially in closed photobioreactors its biomass may be additionally enriched with some trace elements such as iron, iodine, selenium, zinc, copper, manganese and chromium in high bioavailable form. Said biomass is considered as a constituent of new bioactive food supplements.  相似文献   

20.
采用响应面法研究发芽时间、培养液pH和亚硒酸钠浓度对青稞籽粒中有机硒含量的影响,得到最佳富硒发芽工艺条件。在此条件下,进一步对青稞籽粒发芽过程中抗氧化物质含量及抗氧化能力进行分析。结果表明:青稞籽粒最佳富硒发芽工艺为发芽时间48.00 h、pH6.00、亚硒酸钠浓度10.00 mg/L,在此条件下,青稞籽粒有机硒含量为1.572 mg/kg DW,为原料的17.46倍。富硒发芽可显著提高青稞籽粒中总酚、总黄酮醇、有机硒含量以及ABTS+自由基清除能力(p<0.05),尽管降低了β-葡聚糖含量及铁离子还原能力(FRAP),但与未富硒组相比,富硒可有效延缓β-葡聚糖降解。相关性分析表明,总酚含量与有机硒含量、ABTS+自由基清除能力,β-葡聚糖含量与铁离子还原能力(FRAP)以及有机硒含量与ABTS+自由基清除能力均呈极显著正相关(p<0.01),其他指标间呈负相关或不相关。本研究表明,富硒处理可增加发芽青稞籽粒抗氧化物质含量,有效延缓β-葡聚糖降解,增强其抗氧化能力。  相似文献   

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