Responses of aquatic organisms to metal pollution in a lowland river in Flanders: a comparison of diatoms and macroinvertebrates

The role of macroinvertebrates and diatoms as indicator for metal pollution was investigated by assessing both biota along a metal gradient in the Belgian river the Dommel. Macroinvertebrates and diatoms were sampled in summer and winter and physical-chemical characteristics of the water were measured at four different sample periods and related to sediment characteristics. Although metal concentrations, except cadmium, in the water nowhere exceeded water quality standards, high metal concentrations were measured in the sediment, indicating historical contamination of the Dommel. At the sites that were situated downstream of the pollution source, high levels of conductivity and chloride were measured in the water. Redundancy Analysis (RDA) indicated pH, phosphate and zinc as the significant environmental variables explaining each respectively 7.7%, 11.6% and 22.6% of the macroinvertebrate community composition. Two clusters could be separated, with Gammarus pulex, Leptocerus interruptus, Baetis rhodani and Cloeon dipterum associated with low zinc concentrations and Tubificidae, Asellus aquaticus, Erpobdella sp. and Chironomus thummi-plumosus associated with higher zinc concentrations. Ammonium (10.6%), conductivity (16.5%), chloride (11.4%) and zinc (5.9%) turned out to be significant variables explaining the diatom community structure. Based on physical-chemical differences and species composition, three different groups could be separated. With this Tabellaria flocculosa and Fragilaria capucina var. rumpens were associated with low metal concentrations, Gomphonema parvulum and Nitzschia palea with elevated concentrations and Eolimna minima and Sellaphora seminulum with high zinc concentrations. In conclusion, the diatom community best reflected the metal gradient. With regard to water quality indices, those based on macroinvertebrates best followed the metal pollution gradient and were most strongly correlated with physical-chemical variables of water and sediment. This study indicated that to assess the effect of metal pollution in lowland rivers, the combined use of macroinvertebrates and diatoms is more appropriate than the use of both biota separately.     

The impact of cattle farming best management practices on surface water nutrient concentrations,faecal bacteria and algal dominance in the Lake Oconee watershed

The objective of this study was to assess water quality in the Lake Oconee watershed and evaluate the best management practices used by cattle farms to reduce water contamination. Inorganic nutrient concentrations, algal abundance and faecal bacteria were highest in the cattle farming areas. The diatom community where cattle had no access was dominated by Achnanthidium minutissimum (Kützing) Czarnecki and Fragilaria crotonensis Kitton, and in sites where cattle were allowed direct access to the lake, Asterionella formosa Hassal, Nitzschia palea (Kützing) Smith and Navicula rostellata Kützing dominated. The latter three taxa are well‐known high‐nutrient diatoms. High populations of green algae (coccoid Desmidiaceae) were found where cattle had access. Sources of faecal pollution were identified using polymerase chain reaction detection, with Bifidobacterium adolescentis as a marker of human faecal pollution and Bacteroides (BoBac) indicating cattle faecal pollution. Overall, riparian buffers were most effective at reducing pollution from cattle operations.     

Field transfer of periphytic diatom communities to assess short-term structural effects of metals (Cd,Zn) in rivers

Short-term effects of metal pollution were assessed by transferring periphytic diatom communities developed on artificial substrates from a reference site to a site polluted by heavy metals (around 15 microg Cd L(-1) and 800 microg Zn L(-1)), located in neighbouring streams. Metal sensitivity of mature assemblages (aged 1 month) was evaluated by studying changes in diatom density and taxonomic composition 2 and 4 weeks after the transfer. Resident communities of both sites were simultaneously collected on similar artificial substrates, in order to compare short-term effects of metals within transferred communities with long-term effects observed at the polluted site. Field transfer induced a marked shift of community structure towards that of the resident community of the polluted site: diatom density rapidly decreased after the transfer, from 35,000+/-4000 to 15,000+/-300 cells cm(-2) after 2 weeks, and taxonomic composition changed. Relative abundances of species characteristic of the reference site rapidly decreased, e.g. Nitzschia dissipata and Gomphoneis minuta, whereas species characteristic of the polluted site progressively increased within transferred communities, e.g. Gomphonema parvulum, Pinnularia sp. or Fragilaria crotonensis. The structure of periphytic diatom communities could therefore be an indicator of metal pollution, with marked taxonomic changes being identified within reference assemblages after only 2 weeks under relatively low metal exposure.     

Viral pollution determination of superficial waters (river water and sea-water) from the urban area of Barcelona (Spain)

Various surface water systems in the metropolitan area of Barcelona (Spain), including two rivers and three beaches, were analysed for human viruses using glass powder as adsorbent to concentrate them. The method used allows concentration of large volumes (up to 2001), of water in a single step. Values obtained varied from 18.4 to values higher than 55 MPNCU 1⁻¹in the Besôs river and 0.44-44 MPNCU 1⁻¹in the Llobregat river. Viruses were isolated from approx. 70% of sea-water samples, the values ranging from 0 to 1.60 MPNCU 1⁻¹. Findings are discussed in terms of correlation between virus pollution and bacterial indicators of fecal contamination. Also, the suitability of glass powder adsorption to concentrate viruses in sea-water is discussed.     

Modelling of BOD-DO dynamics in an ice-covered river in Northern China

BOD-DO dynamics in a river in northern China during a period of sub-zero temperatures have been investigated and a mathematical model which describes the dynamics has been proposed. The model parameters: deoxygenation rate k₁, reaeration rate k₂, BOD sedimentation—resuspension rate k₃ were determined by a parameter estimation method using the field data collected on Tu Men River. Validation of the model shows that BOD-DO dynamics in the river during that season are well described by the model. The model offers a tool for understanding the natural purification process in the rivers in northern China under ice-cover conditions and also for control of water quality in those rivers.     

Toxicity and sorption kinetics of dissolved cadmium and chromium III on tropical freshwater phytoperiphyton in laboratory mesocosm experiments

The objective of this study was to assess the interactive effects of Cd and Cr III on tropical phytoperiphyton community growth, metal sorption kinetics, as well as Cd and Cr mixtures toxicity to diatom assemblages in laboratory mesocosm experiments. A natural phytoperiphyton community sampled from the Monjolinho River (South of Brazil) was inoculated into seven experimental systems containing clean glass substrates for phytoperiphyton colonization. The communities were exposed to mixtures of dissolved Cd and Cr concentrations of 0.01 and 0.1 mg.L⁻¹ Cd and 0.05 and 0.2 mg.L⁻¹ Cr. Phytoperiphyton chlorophyll a, ash-free dry mass, growth rate, diatom cell density and diatom community composition were analyzed on samples collected after 1, 2 and 3 weeks of colonization. High Cd concentration (0.1 mg.L⁻¹) affects phytoperiphyton growth while high concentration of Cr (0.2 mg.L⁻¹) decreased the toxic effects of Cd on phytoperiphyton growth demonstrating the importance of studying metal mixtures in field studies. Shifts in species composition (development of more resistant species like Achnanthidium minutissimum (Kützing) Czarnecki, and Nitzschia palea (Kützing) Smith and reduction of sensitive ones like Fragilaria capucina Desmazières, Navicula cryptocephala (Grunow) Cleve, Encyonema silesiacum (Bleisch) Mann, Eunotia bilunaris (Ehrenberg) Mills and Gomphonema parvulum (Kützing) Kützing), of phytoperiphyton communities with increasing Cd and Cr concentrations and exposure duration have been demonstrated in this study making phytoperiphyton communities appropriate monitors of metal mixtures in aquatic systems. Good Cd and Cr accumulation capacity by phytoperiphyton was demonstrated with total and intracellular metal content in phytoperiphyton reflecting the effects of dissolved concentrations of metal in the culture media and exposure duration. Increase in both Cd and Cr reduced sequestration of each other, with generally more Cd being sequestered compared to Cr. Field validation of the observed effects remains an interesting subject for further investigations.     

Diatom assemblages distribution in catalan rivers, NE Spain, in relation to chemical and physiographical factors

Distribution patterns of epilithic diatom assemblages in streams in northeastern Spain and their relation to different environmental gradients are presented. Thirty-five sites were sampled covering a wide range of fluvial typologies. Gradient analysis was used to analyze the community structure and the major ecological gradients underlying variation in species composition. Two major gradients were evident; the first was a complex gradient from oligotrophic, pristine, fast-flowing highland rivers to mainly eutrophic rivers of low elevation; and the second related to altitudinal and seasonal variation of temperature. Two sets of factors were evident; one was associated with water chemistry, and the other to seasonal and physiographical variation. Variance partitioning allowed the separation of the effects of the different sets of environmental parameters. The contribution of physiography and water chemistry to diatom distribution was more unclear when the level of disturbance was intermediate. Considering the relatively high proportion of variation explained by physiographical variables alone, we suggest that when using diatoms to evaluate water quality, ecoregional characteristics of river stretches should also be considered.     

Study of changes in concentration during penetration through the soil layers by means of co-relationship

This report presents a co-relationship method that is used to evaluate changes in water quality. The co-relationship based on the water quality in the Goto river basin, is defined as plots of concentration of dissolved matter against total residue on evaporation, the correlation coefficient and the regression equation. SiO₂ and COD concentrations in the water samples of the Goto river basin show a peculiar change that is independent of the total residue on evaporation and flow length, and this change is again found by means of the co-relationship. The peculiar change of SiO₂ is also observed in some rivers in Japan. The correlation coefficient of the total residue on evaporation and SiO₂ is about 0.2–0.6 in the Goto river basin and in some other rivers in Japan, and the intercept of the regression equation of SiO₂ and Cl⁻ is apt to show a plus value in SiO₂ but a minus value in Cl⁻ in most of the co-relationships. The plus value of the intercept and the gentle slope of SiO₂ show that SiO₂ concentration is independent from that of the total residue on evaporation, and the minus value of Cl⁻, shows that a certain value of total residue on evaporation corresponds to zero concentration of Cl⁻, which means Cl⁻ does not dissolve out from the soil colloid. Thus, the present report suggests that there is a peculiar behavior in some species in the water flow by means of the co-relationship.     

Multi-biochemical responses of benthic macroinvertebrate species as a complementary tool to diagnose the cause of community impairment in polluted rivers

Biological indexes, based on benthic macroinvertebrate taxa, are currently used worldwide to measure river ecological quality. These indexes assign a global ecological status of the biotic community, but not necessarily may detect specific effects of water pollutants. Conversely a large set of biochemical markers measured in macroinvertebrate benthic species can detect sublethal effects and inform us about additional environmental factors that are impairing benthic communities. This is especially interesting in moderately polluted sites, where other stressors are already affecting communities but not too strongly to be detected by biotic indexes. Up to ten different markers belonging to distinct metabolic paths and 42 contaminants measured in sample collections of the caddis fly Hydropsyche exocellata were assessed across a polluted gradient in the industrialized Mediterranean River basins of Besós and Llobregat (NE, Spain). Twenty four sample collections were selected to include macroinvertebrate communities representing the five impairment degrees defined by the Spanish Environmental authorities using the biotic metrics. Results evidenced a clear deterioration of the ecological water quality parameters and benthic communities towards downstream reaches. Biochemical responses varied significantly across the studied samples and were able to differentiate samples within communities having a good and deteriorated ecological stage. Principal Component Analyses indicated that salinity was one of the major stresses affecting macroinvertebrate assemblages, whereas antioxidant and metabolizing enzymes responded differently and were closely related to high and presumably toxic levels of accumulated organic pollutants. Therefore these results indicate that the use of multiple -markers sensitive to water pollution may provide complementary information to diagnose environmental factors that are impairing macroinvertebrate communities.     

Typology of diatom communities and the influence of hydro-ecoregions: a study on the French hydrosystem scale

By comparing diatom communities in natural and disturbed sites, indicators for different types and levels of anthropogenic disturbance can be found. As a first step, this study aims to describe the different natural and disturbed community types found throughout the French hydrosystem. 836 diatom samples were analysed with an unsupervised neural network, the self-organising-map, a well accepted method for community ordination. 11 different communities were identified, 5 corresponding to non-impacted or slightly impacted conditions and representing the diatom natural variability of our dataset. These 5 communities corresponded to 5 different hydro-ecoregions, i.e. 5 river types with similar geological context and range in altitude. The 6 other communities were typical of rivers under anthropogenic pressure. The influence of natural conditions within the hydro-ecoregions was overwhelmed by the nature and the intensity of the pollution at the sampling stations. This work was done in the context of the application and enforcement of the Water Framework Directive.     

Quality of dredged material in the river Seine basin (France). II. Micropollutants

Dredging rivers is needed to ensure safe navigable waters, rivers and waterways. To anticipate the management of dredged materials in the case of the river Seine basin, the quality of the sediments in the river is checked every 3 years before dredging operations. The river Seine Basin is heavily submitted to pollution pressure from nearby industrial activities and urban expansion of Paris and its region. Here, the micropollutant content of the sediment sampled in 1996, 1999 and 2000 before dredging is discussed compared to regulatory standards. The results indicate that most of the sediment samples from the river Seine basin are lightly to moderately contaminated with organic and inorganic micropollutants (heavy metals, PAH, PCB), which makes the management after dredging easier. This pollution is strongly correlated with the organic matter content and to the fine fraction (<50 microm) of the sediment. These results can lead to other management options than the ones already used in the river Seine basin: (1) dumping of lightly to moderately polluted sediments in quarries; and (2) physical treatment (sieving, hydrocycloning) of contaminated sediments issued from 'hot spots'.     

The Impact of Intensive Dairy Farming Activities on River Quality: The Eastern Cleddau Catchment Study

Pollution from farm wastes has been one of the principal causes of deterioration in river quality in recent years. However, little is understood of how, and which, farming activities affect the chemical and biological quality of rivers. To address this problem, a field study has been initiated in the Eastern Cleddau catchment, West Wales, to investigate relationships between land use, farm waste management practices and river quality.
Water quality of small tributaries in this area is poor, and intensive chemical monitoring has shown that discharges from farmyards are a major source of pollution. Rainfall has been shown to exacerbate this effect either through field runoff or wastes washing directly from the farmyards. The status of benthic macroinvertebrate communities in these tributaries is poor and, typically, only a few pollution-tolerant species are present.
Small tributaries from farms appear to affect both the water chemistry and biology of larger watercourses, although this effect may be quite localized.
Future research will look at ways to reduce the impact of farm wastes on river quality. This will provide the basis for producing recommendations and guidelines for catchment management aimed at minimizing pollution of rivers from farming practices.     

TheGammarus: Asellus ratio as an index of organic pollution

Macroinvertebrate populations includingGammarus pulex andAsellus aquaticus were surveyed at riffle sites on four lowland rivers, i.e. the Rivers Adur and Ouse and the Chess Stream, Sussex, and the Eridge Stream, Kent. Sites were situated both upstream and downstream of sewage treatment works. Macroinvertebrates were collected using a Surber sampler and at some sites the water analysed for the main chemical constituents.Poor water quality is characteristically associated with low biotic scores and in this respect theGammarus : Asellus ratio applied to riffle data corresponded with the Chandler Biotic Score Index and the Extended Trent Biotic Index. TheGammarus : Asellus ratio showed the closest correlations with concentrations of BOD, ammonia-nitrogen (NH₃-N), nitrite-nitrogen (NO₂-N) and phosphate-phosphorus (PO₄-P) being sensitive to changes in water quality brought about by organic enrichment in the four rivers investigated.It is proposed that theGammarus : Asellus ratio may provide a simple biological tool which could be used by anglers and other non-professional river users to routinely monitor water quality.     

Carbon source utilization‐based metabolic activity of Salmonella Oranienburg and Salmonella Saintpaul in river water

The presence of introduced or non‐native bacteria in river water can create a selective pressure due to their ability to overcome fluctuations in physicochemical conditions and carbon source availability. The carbon source concentrations (monosaccharides and polysaccharides), physicochemical parameters (turbidity, total dissolved solids, biochemical oxygen demand, temperature and pH) and metabolic activity of Salmonella Oranienburg and Saintpaul were determined in water from the central Sinaloa state rivers. This study's results revealed that the carbohydrate availability and physicochemical conditions of river water make it a suitable niche for the establishment of Salmonella serotypes. Metabolic profiles showed that Salmonella Typhimurium could consume a wide variety of carbon sources but only at moderate levels; in contrast, Salmonella Oranienburg and Salmonella Saintpaul demonstrated intense carbon source utilization of a limited diversity of carbon sources. Carbohydrates, amino acids and carboxylic acids were the metabolites most utilized by the environmental Salmonella strains, demonstrating their superior ability to adapt to and survive in river water.     

Hydrology and the ecological quality of Scottish river ecosystems

Hydrology is a primary control on the ecological quality of river systems, through its influence on flow, channel geomorphology, water quality and habitat availability. Scottish rivers are widely perceived to be of high ecological quality, with abundant flow volumes and high water quality. However, historical and current river flow regulations, and land use change have altered the physical and chemical characteristics of Scottish rivers, with adverse consequences for aquatic biota. Baseline hydrological, geomorphological and water quality conditions in Scottish rivers are thus summarised. The impacts of river regulation and land use change on the hydrology, geomorphology and water quality of Scottish rivers are then discussed. Consequences of these changes for aquatic habitat are examined, with particular reference to the economically significant salmonid species (Salmo salar and Salmo trutta). Policy and management issues relating to the future ecological quality of Scottish rivers are reviewed. These include the impacts of climate change on ecological quality, the calculation and implementation of ecologically acceptable flows, and river restoration and best management practices within integrated catchment planning.     

Toxicity of lipid-soluble copper(II) complexes to the marine diatom Nitzschia closterium: Amelioration by humic substances

Algal assays, using the marine diatom Nitzschia closterium, have established that humic acid (5 mg kg⁻¹) can ameliorate the toxicity of the lipophilic complex Cu(oxine)₂ (3 × 10⁻⁸ mol l⁻¹ in unsupplemented seawater). The toxicity of Cu(PAN)₂ is not ameliorated [PAN = 1-(2-pyridylazo)-2-napthol]. In conjunction with previous visible absorption spectrophotometry and polarographic measurements it was established that humic acid sequesters copper(II) from the hydrophobic complexes, releasing a ligand molecule. The copper(II) toxicity may be ameliorated provided the ligand itself is not toxic.Fulvic acid was significantly less effective in ameliorating toxicity. Because of the significant competition from Ca(II) and Mg(II) in seawater, it is inferred that humic substances may be more effective in ameliorating toxicity of hydrophobic copper complexes in fresh water.     

Experimental toxicity and bioaccumulation of cadmium in freshwater periphytic diatoms in relation with biofilm maturity

A study was undertaken to examine cadmium accumulation in freshwater biofilm, its effects on biofilm development and on diatom community structure in laboratory experimental conditions. A suspension of a biofilm originated from the Riou-Mort River (South West France) was inoculated into three experimental units containing clean glass substrates under laboratory conditions. Settling and already developed biofilms were exposed to a Cd concentration of 100 µg L^− 1. Metal accumulation (total and intracellular metal content) in biofilms, dry weight and ash-free dry mass, diatom cell density and diatom community composition were analyzed. Both total and intracellular Cd accumulated by the biofilm throughout the experiment increased with duration of metal exposure. Biofilms in the course of maturation were showed higher Cd content and less effective development than settled biofilms. However diatom communities in younger biofilms exposed to Cd increased their tolerance to Cd by a highly significant development of Nitzschia palea. In contrast, Cd exposure had different effect in installed biofilm and taxonomic composition. These results indicate that mature biofilm may limit Cd accumulation into its architecture and protect diatom communities from the effects of metals.     

The British river of the future: How climate change and human activity might affect two contrasting river ecosystems in England

The possible effects of changing climate on a southern and a north-eastern English river (the Thames and the Yorkshire Ouse, respectively) were examined in relation to water and ecological quality throughout the food web. The CLASSIC hydrological model, driven by output from the Hadley Centre climate model (HadCM3), based on IPCC low and high CO₂ emission scenarios for 2080 were used as the basis for the analysis. Compared to current conditions, the CLASSIC model predicted lower flows for both rivers, in all seasons except winter. Such an outcome would lead to longer residence times (by up to a month in the Thames), with nutrient, organic and biological contaminant concentrations elevated by 70-100% pro-rata, assuming sewage treatment effectiveness remains unchanged. Greater opportunities for phytoplankton growth will arise, and this may be significant in the Thames. Warmer winters and milder springs will favour riverine birds and increase the recruitment of many coarse fish species. However, warm, slow-flowing, shallower water would increase the incidence of fish diseases. These changing conditions would make southern UK rivers in general a less favourable habitat for some species of fish, such as the Atlantic salmon (Salmo salar). Accidental or deliberate, introductions of alien macrophytes and fish may change the range of species in the rivers. In some areas, it is possible that a concurrence of different pressures may give rise to the temporary loss of ecosystem services, such as providing acceptable quality water for humans and industry. An increasing demand for water in southern England due to an expanding population, a possibly reduced flow due to climate change, together with the Water Framework Directive obligation to maintain water quality, will put extreme pressure on river ecosystems, such as the Thames.     

Algal diversity in relation to water quality in an Indian river

The application of the two diversity indices (Shannon's index of diversity and Margalef s index of diversity) has been critically analysed for water pollution monitoring in the tropical polluted river Khan. These two indices can be usefully employed for the biological assessment of water quality in tropical polluted rivers.