紫外光谱在研究金属配合物与DNA相互作用中的应用

概述了紫外光谱法在研究金属配合物与DNA相互作用中的应用。紫外光谱法是一种研究配合物与DNA相互作用最简便、常用而有效的方法,不仅能够判断配合物与DNA间的作用模式,还能够准确表征两者间的作用强度,在化合物与DNA相互作用研究中发挥着重要作用。     

壳寡糖席夫碱Cu(Ⅱ)配合物与鲱鱼精DNA的相互作用?

以壳寡糖与对羟基苯甲醛反应合成的席夫碱化合物作为配体,构建了一系列的金属Cu(Ⅱ)配合物,并通过紫外光谱法、电化学手段以及粘度实验,初步研究了配合物与DNA的作用机理。紫外光谱实验发现,配合物的特征吸收峰在加入DNA后峰位均呈现红移和减色;从循环伏安曲线可见,DNA加入后配合物的氧化峰电流呈下降趋势,峰电位伴随有正移趋势;DNA的粘度随着配合物浓度的依次增大,呈先增大后降低的趋势。结合以上这些信息,认为配合物与DNA以部分嵌插方式作用的。     

6-O-羧甲基氨基葡萄糖铁配合物的制备及其与DNA相互作用研究

利用D-氨基葡萄糖与氯乙酸反应合成了6-O-羧甲基D-氨基葡萄糖(CMG),结合中性辅助配体2,2′-联吡啶,合成了一种新型铁金属配合物;采用红外光谱对配体及配合物的结构进行表征;采用紫外可见吸收光谱法、荧光光谱法和黏度法研究了新型配合物与鲱鱼精DNA的相互作用。结果表明新型配合物可以与鲱鱼精DNA以嵌插方式结合,从而使DNA双螺旋结构破坏。     

三齿多吡啶钴(Ⅱ)配合物与DNA的相互作用研究

合成了2种三齿多吡啶钴(Ⅱ)配合物,用元素分析、IR等对它们进行了表征,用循环伏安法、电子吸收光谱、荧光光谱等方法研究了配合物与CT DNA的相互作用以及配合物对pBR322DNA的断裂作用.结果表明,配合物[Co(PhTPY)2]2+与DNA的作用属中等强度的部分插入,配合物[Co(H2 Bzimpy)2]2+与DNA的作用属静电作用.凝胶电泳实验表明,用310 nm光辐射15 min,配合物[Co(PhTPY)2]2+可选择性地断裂pBR322DNA为开环缺口性和线型DNA,配合物[Co(H2 Bzimpy)2]2+对DNA的断裂没有选择性.     

芳香族氨基酸二肽金属配合物与DNA作用研究

芳香族氨基酸二肽类配体,具有特殊的芳香性侧链结构、良好的理化性质及生理相容性等优势,使芳香族氨基酸二肽金属配合物成为新型金属配合物抗癌药物研究的热点。通过系统研究芳香族氨基酸二肽金属配合物与DNA作用,可为设计、开发新型抗癌药物奠定基础。重点综述芳香族氨基酸直链二肽类金属配合物、芳香族氨基酸环二肽类金属配合物和芳香族氨基酸二肽有机金属配合物与DNA作用的研究进展。     

三齿仲胺配合物的合成、表征及生物活性的研究

合成了一种三齿配体N-(4-甲基苯)-N′-(2-(4-甲基苯氨基)乙基)乙烷-1,2-二胺,并制备了它的3种过渡金属配合物[ML(H2O)2Cl]Cl(M=CuⅡ,CoⅡ,NiⅡ),用元素分析、核磁、红外和摩尔电导等表征了配体及其金属配合物的组成和结构。对Ni—L进行了单晶结构解析,配合物为六配位的八面体结构,配体采用三齿配位。利用紫外光谱和荧光光谱法研究了配合物与小牛胸腺DNA的作用方式,结果表明,3种金属配合物可能以静电方式与DNA作用。Cu—L、Ni—L、Co—L3种配合物与DNA的结合常数分别为2.99×104、9.78×103、3.66×103。     

多核铜槲皮素配合物对DNA的作用研究

应用紫外光谱、凝胶电泳法实验探究了3种多核铜槲皮素配合物与DNA的作用形式及对DNA的裂解能力。结果显示,3种配合物对DNA的作用效果强于槲皮素,配合物通过插入、静电、剪切多种形式与DNA作用,具有高效的DNA断裂活性。     

Synthesis and DNA Binding Properties of a Water-Soluble CHT-Co（Ⅱ） Complex

以1,10-菲罗啉、壳聚糖和四水合乙酸钴为原料,合成了水溶性壳聚糖钴（II）配合物（CHT-Co（II））,采用红外光谱对配体及配合物进行了表征。在pH 7.5的Tris-HCl缓冲溶液中,采用粘度法、紫外可见吸收光谱法和电化学方法（循环伏安法,微分脉冲伏安法）研究了配合物与DNA的相互作用。粘度法表明二者能发生部分嵌插作用;DNA的加入能引起配合物紫外光谱的变化也表明了二者之间的相互作用。电化学实验表明配合物在玻碳电极上的氧化还原为一可逆过程,当加入一定量的DNA时,配合物的氧化峰电流明显降低,式电位有所正移,验证了二者之间存在部分嵌插作用。     

[Ce(LA)_2(NO_3)_3]的晶体结构、抗肿瘤活性以及与DNA作用研究

以鹅掌楸碱为配体与稀土金属铈反应合成配合物[Ce(LA)_2(NO_3)_3],通过X-射线单晶衍射对该配合物进行结构表征,并对其进行肿瘤细胞的体外抑制活性筛选。采用紫外-可见光谱、粘度实验研究该金属配合物与DNA的作用机制。     

多吡啶铜(Ⅱ)配合物的合成、表征与DNA作用及抗肿瘤活性

合成了一种多吡啶铜(Ⅱ)配合物,用红外光谱、元素分析、X-射线单晶衍射法对配合物的组成和结构进行了表征。该配合物的晶体属单斜晶系,P2(1)/c空间群。用荧光光谱、紫外光谱研究了配合物与小牛胸腺DNA的键合作用。结果表明,配合物与DNA的键合作用属部分插入,结合常数K_b=8.0×104L/mol。用凝胶电泳法研究了配合物对p BR322DNA的切割作用。实验表明,配合物用310 nm光辐射15 min,可将p BR322 DNA断裂为开口缺刻型和线型DNA。采用MTT法研究了配合物对人乳腺癌细胞和肺癌细胞的体外抗肿瘤活性。结果发现,所合成配合物能有效抑制这两种细胞增殖。     

Effect of Copper(II) Ion Binding by Porin P1 Precursor Fragments from Fusobacterium nucleatum on DNA Degradation

Fusobacterium nucleatum is one of the most notorious species involved in colorectal cancer. It was reported that numerous outer membrane proteins (OMP) are actively involved in carcinogenesis. In this paper, the structure and stability of certain complexes, as well as DNA cleavage and ROS generation by fragments of OMP, were investigated using experimental and theoretical methods. Mass spectrometry, potentiometry, UV-Vis, CD, EPR, gel electrophoresis and calculations at the density functional theory (DFT) level were applied. Two consecutive model peptides, Ac-AKGHEHQLE-NH₂ and Ac-FGEHEHGRD-NH₂, were studied. Both of these were rendered to form a variety of thermodynamically stable complexes with copper(II) ions. All of the complexes were stabilized, mainly due to interactions of metal with nitrogen and oxygen donor atoms, as well as rich hydrogen bond networks. It was also concluded that these complexes in the presence of hydrogen peroxide or ascorbic acid can effectively produce hydroxyl radicals and have an ability to cleave the DNA strands. Surprisingly, the second studied ligand at the micromolar concentration range causes overall DNA degradation.     

多功能咔咯配合物的合成与应用进展

咔咯(Corrole)是由4个吡咯共轭相连而形成的新型卟啉类大环配合物,它们能稳定结合具有较高氧化态的金属离子,所形成的金属配合物具有比较独特的光化学、电化学、光物理和光生物性质。研究表明,咔咯是一类非常重要的多功能配合物,它们在精细化学品领域具有广阔的应用前景。对咔咯配合物的主要合成方法、配合物用作精细化工催化剂、燃料电池电极催化材料、抗肿瘤光敏剂、电化学传感器和非线性光学材料等方面的研究和应用作了综述。     

Synthesis,crystal structure,DNA binding properties and antioxidant activities of transition metal complexes with 3-carbaldehyde-chromone semicarbazone

3-Carbaldehyde-chromone semicarbazone (L) and its Cu(II), Zn(II), Ni(II) complexes were synthesized and characterized on the basis of crystal structure and other structural characterization methods. The metal ions and Schiff base ligand can form mononuclear five-coordination complexes with 1:1 metal-to-ligand stoichiometry at the metal ions as centres. The transition metal complexes may be used as potential anticancer drugs, because they bind to calf thymus DNA via an intercalation binding mode with the binding constants at the order of magnitude 10⁵–10⁶ M^? 1, and the metal complexes present stronger DNA binding affinities than the free ligand alone. In addition, the antioxidant activities of the ligand and its metal complexes were investigated through scavenging effects for superoxide anion and hydroxyl radical in vitro, indicating that the compounds show stronger antioxidant activities than some standard antioxidants, such as mannitol and vitamin C.     

氨基酸类希夫碱配合物的性能及对DNA作用机理研究

氨基酸希夫碱配合物是一类生物活性物质,具有抗肿瘤、杀菌、抑制癌细胞作用,其衍生物可作为人造核酸酶和有效的DNA切割试剂。这类配合物与DNA的相互作用可以从分子水平认识特定DNA系列、改变DNA结构和影响基因表达过程进行医学治疗诊断。介绍了醛缩、酮缩氨基酸希夫碱配合物的类型、合成方法、性质和生物作用。分析了氨基酸希夫碱参与过渡金属、稀土金属配位成键的方式和空间构型。讨论了标题化合物与DNA之间的作用机理。     

Impact of metallonucleases on DNA interactions: Structural validation and in-vitro antibiogram assay

Four novel complexes were synthesized using 2-aminobenzophenone Schiff base precursor and characterized by various analytical techniques. FT-IR results demonstrate that the Schiff base ligand, obtained by the condensation of 2-aminobenzophenone with 2-hydroxybenzaldehyde, coordinates to the metal ion via azomethine nitrogen, phenolic oxygen and carbonyl groups. Furthermore, the intercalative mode of these octahedral complexes with calf thymus (CT) DNA has been explored using absorption spectra, cyclic voltammetric and viscosity measurements. Gel electrophoresis assay demonstrates the effective cleaving aptitude of pBR322 DNA by complexes in the presence of an activating agent (H₂O₂). Moreover, the in-vitro biological activities of metal complexes have been screened against few pathogenic bacterial and fungal strains by well diffusion technique. It is found that all the complexes show potential activities as compared to the free ligand. These findings may lead to develop novel DNA targeting metallonucleases.     

DNA-Targeted Metallodrugs: An Untapped Source of Artificial Gene Editing Technology

DNA binding metal complexes are synonymous with anticancer drug discovery. Given the array of structural and chemical reactivity properties available through careful design, metal complexes have been directed to bind nucleic acid structures through covalent or noncovalent binding modes. Several recognition modes – including crosslinking, intercalation, and oxidation – are central to the clinical success of broad-spectrum anticancer metallodrugs. However, recent progress in nucleic acid click chemistry coupled with advancement in our understanding of metal complex–nucleic acid interactions has opened up new avenues in genetic engineering and targeted therapies. Several of these applications are enabled by the hybridisation of oligonucleotide or polyamine probes to discrete metal complexes, which facilitate site-specific reactivity at the nucleic acid interface under the guidance of the probe. This Review focuses on recent advancements in hybrid design and, by way of an introduction to this topic, we provide a detailed overview of nucleic acid structures and metal complex–nucleic acid interactions. Our aim is to provide readers with an insight on the rational design of metal complexes with DNA recognition properties and an understanding of how the sequence-specific targeting of these interactions can be achieved for gene engineering applications.     

过渡金属配合物与DNA作用的研究状况和应用

文章对过渡金属配合物与DNA相互作用的研究和应用进行了归纳和分析,在过渡金属配合物可用于DNA分子光开关、DNA二级结构探针、DNA错配碱基识别探针、DNA切割试剂等方面进行了总结和探索。     

甘油金属配合物的合成、应用及发展前景

甘油金属配合物是一种重要的精细化工产品，它能以稳定的多种形式使用，无毒、无公害，在高分子、医疗卫生、化妆品和无机材料等多个行业广泛应用。介绍了甘油金属配合物的制备及应用，为甘油金属配合物的开发研究与推广应用提供帮助。     

Studies on the interaction of DNA and water‐soluble polymeric Schiff base–nickel complexes

The modes and activities of the interaction of DNA and water‐soluble polymeric Schiff base–nickel complexes, which have been prepared by polymeric analogous reaction, have been discussed according to absorption spectra, circular dichroism spectra, and fluorescent probe method. It is indicated that polymeric matrix effect and increasing solubility in water can increase the interaction of these water‐soluble polymeric metal complexes with DNA. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 84: 887–893, 2002; DOI 10.1002/app.10000     

金属配合物抗癌活性的研究进展

自从发现顺铂具有抗癌活性以来,金属配合物作为新一类抗癌药物引起了人们的广泛关注。综述了近年来铂类配合物、钌类配合物和席夫碱配合物在抗癌药物中的研究进展,重点阐述了铂类配合物抗癌活性的研究现状及作用原理,并对金属配合物抗癌活性的研究进展进行总结,为金属配合物抗癌药物研究提供参考和依据。