RELATIONSHIP BETWEEN INSTRUMENTAL AND VISUAL COLOR IN A RAW, FRESH BEEF AND CHICKEN MODEL SYSTEM

A range of meat redness was created by mixing fresh, chilled ground chicken breast and ground beef in predetermined ratios. Samples were scored for visual redness under halogen (incandescent) and cool white fluorescent lighting. Instrumental color characteristics (L*, a*, b*, hue angle, chroma; illuminants A and F) and red color contributed by oxymyoglobin (percentage reflectance at 630 nm versus 580 nm) were determined. Correlation coefficients were determined between visual and instrumental evaluations. The a*, b*, and ΔR (630–580) were highly correlated to visual redness (halogen light: r = 0.99, -0.99, and 0.96, respectively; cool white fluorescent light: 0.98, -0.98, and 0.96, respectively). Regression analysis was conducted to develop equations to predict visual redness using instrumental color measures. In the 20% to 85% beef range, the regression curves were principally linear although the overall relationships (0–100% beef range) were cubic. Among the instrumental color parameters, a*, hue angle, and ΔR (630–580) could be used to predict visual redness for this model system.     

Contribution of pigment content, myoglobin forms and internal reflectance to the colour of pork loin and ham from pure breed pigs

The colour of loin, M. longissimus dorsi (LD), and ham, M. biceps femoris (BF), from pure breed Hampshire, Swedish Landrace and Swedish Yorkshire pigs was studied. The contribution of the pigment content, the myoglobin forms deoxymyoglobin (Mb), oxymyoglobin (MbO) and metmyoglobin (MetMb) and the internal reflectance to the colour of pork of normal meat quality was evaluated using partial least squares regression (PLS). The colour of LD and BF from the Hampshire breed was more red and yellow and more saturated than the colour of the same muscles from the Swedish Landrace and the Swedish Yorkshire breeds. Furthermore, BF from Hampshire was darker than BF from the other two breeds. These differences in colour were related to the lower pH in Hampshire, resulting in more blooming and in higher internal reflectance, and to the higher pigment content. The colour of BF was darker and more red than the colour of LD within each breed. No colour difference was found between gilts and castrates within each breed. Most of the variation (86-90%) in lightness (L* value), redness (a* value) and yellowness (b* value), chroma (saturation) and hue angle of pork of normal meat quality was explained by the pigment content, myoglobin forms and internal reflectance. The L* value, a* value, chroma and hue angle were influenced by both the pigment content and by the myoglobin forms to almost the same extent, while the internal reflectance was of no significance to these colour parameters. The b* value was influenced most by the myoglobin forms, less by the internal reflectance and almost not at all by the pigment content.     

Evaluation of Venison Color by an Objective Method using CIELAB Values

A trained color panel was used to score both venison color and color acceptability for comparison with instrumental color measurements. Panel color scores were highly correlated with acceptability (r = 0.97) and could be predicted from CIE L*, a* and b* values by regression (R² (adj) = 0.84). The use of a* alone explained the greatest amount of variation R² (adj) = 0.78), and the inclusion of both L* and b* significantly improved the model. The use of hue angle and chroma either individually or in combination with L* did not provide better relationships. The use of L*, a* and b* is recommended for satisfactory prediction of panel color scores of venison with no less than three observations per sample.     

Instrumental evaluation of pH effects on ability of pork chops to bloom

Color plays an important role in consumer purchase decisions of pork. For this reason, the objective of this study was to determine the usefulness of instrumental measures of bloom and bloom as a function of pH. Color was measured with two instruments on pork chops of varied ultimate pH (5.1–6.1) in a vacuum package and again after blooming. During bloom, Hunter a* increased while Minolta a values changed little. Bloom had no effect on either measure of lightness (L* or L). With bloom, Hunter Hue Angle decreased indicating an increase in red color, while Minolta Hue Angle measurement increased with bloom, implying a decrease in true red color. Although lightness was unaffected by bloom, pH had a significant effect on Hunter L* and Minolta L values. Increasing pH resulted in an increase of Hunter a*, but not Minolta a, in both unbloomed and bloomed chops. Hunter Hue Angle was consistently higher than Minolta Hue Angle regardless of bloom status or pH.     

Quality changes in beef gluteus medius, infraspinatus, psoas major, rectus femoris, and teres major enhanced prior to aging

ABSTRACT:  The objective of this study was to evaluate the effects of enhancement and aging on quality characteristics of the gluteus medius (round), rectus femoris (round), teres major (chuck), infraspinatus (chuck), and psoas major (loin). Muscles were enhanced, vacuum packaged, and aged for 7 or 14 d. Aging affected enhanced compared with nonenhanced beef differently. After 7 d of aging, enhanced beef experienced more cook loss and was less red (higher hue angle, lower a * values, and chroma) than did nonenhanced beef; after 14 d of aging, these differences were lost. Enhancement increased tenderness and brown color of the gluteus medius (round); however, it decreased visual green and red colors. Enhanced gluteus medius experienced 3.1% lower purge losses, lower L * values, and chroma than their nonenhanced counterparts. Enhancement increased infraspinatus (chuck) tenderness and visual brown color, and decreased visual red and green colors, purge loss, L * value, a * value, and chroma. The enhanced infraspinatus was substantially more tender than the other muscles evaluated, other than the gluteus medius ; however, it was also substantially more visually brown. Enhancement decreased purge loss of the psoas major . Enhancement increased L * and a * values, hue angles, and chroma than other enhanced muscles. After enhancement the tenderness of the rectus femoris increased by 10%, decreased purge losses from 4.5% to 1.7%, and decreased green color by 18%. It reduced L * and a * values and chroma. Aging increased tenderness. Overall, muscles from the chuck ( infraspinatus and teres major ) appeared to benefit the most from enhancement.     

Instrumental reflectance values of fresh pork are dependant on aperture size

The effect of aperture size on instrumental pork color measurements was studied. Pork longissimus chops (n = 30) were cut, aerobically packaged, and allowed to bloom overnight. Chops were measured once with each of four aperture ports (4.45, 2.54, 1.27, and 0.64 cm diameter) on a HunterLab LabScan. Reflectance values measured with smaller aperture sizes were reduced (P < 0.05) for most visual wavelengths (10 nm intervals), and the percent reduction was greatest in the range of the longer, redder wavelengths. Values for L^∗, a^∗, b^∗, and saturation index were reduced (P < 0.05) with decreasing aperture size under illuminants A, C, and D₆₅. Using illuminant A, hue angle increased (P < 0.05) as aperture size decreased. For illuminants C and D₆₅, hue angles were similar (P > 0.05) for the three largest aperture sizes, but negative a^∗ values found with the smallest aperture size skewed the hue angle data.     

CHEMICAL AND COLOR CHARACTERISTICS OF SPANISH DRY-CURED HAM AT THE END OF THE AGING PROCESS

Chemical and color (CIELAB) attributes were measured in three muscles of dry-cured hams: Semimembranosus (SM) , Semitendinosus (ST) and Biceps femoris (BF), at the end of the aging step, which represents the last month of the dry-curing process. SM muscles showed the lowest values for all the chemical and color parameters except the a*/b* ratio while the BF muscles had the highest values except for the same a*/b* ratio. Differences were found in the moisture content, salt concentration, water activity, lightness, chroma, and the a*/b* ratio between the three muscles analysed. BF and ST muscles showed no differences for redness, yellowness and hue. Lightness was highly correlated with moisture and water activity values.     

Optimization of instrumental colour analysis in dry-cured ham

Colour of dry cured ham was measured by instrumental techniques in order to determine the optimum measurement index. Five different colour systems were used: CIE L(?)a(?)b(?), Hunter Lab, CIE L(?)u(?)v(?), xyY and XYZ, each with illuminants D65, C and A, and with both 10 and 2° observer angles. Measurements were taken in both Semimembranosus (SM) and Biceps femoris (BF) muscles. Hunter Lab system with illuminant A gave the most reproducible results within muscles for the three colour parameters evaluated. A further comparison of results obtained with 10 and 2° observer angles of Hunter Lab with illuminant A showed there were no significant differences between the angles. Significant differences between results from SM and BF muscles were found in all the hams, the BF always showing higher values than SM for every colour parameter.     

Effect of moisture content on CIE color values in dried apricots

The color change in dried apricots at various moisture contents was studied. Dried apricots originally at 19.32% moisture were first dried to 15.49% and then rehydrated to 20.10%, 25.21% and finally 30.20% moisture levels. The CIE L*, a*, and b* color variables were measured to determine the color of dried apricots, and C* (chroma) and h* (hue) values were calculated from a* and b* values. A linear relationship was found between the color variables and moisture contents at 15.49-30.20%. As the moisture content increased, the L* (lightness), b* (yellowness), C* and h* color values increased while the a* (redness) value decreased for the dried apricots at all moisture levels. Results showed that the moisture content of dried fruits should be specified once the reflectance color variables are reported.     

Postmortem pH, Muscle, and Refrigerated Storage Effects on Ability of Vacuum-Packaged Pork to Bloom

ABSTRACT: Effects of pH, muscle, and storage time on the ability of vacuum-packaged pork chops to bloom were investigated. Low pH samples exhibited more change in color measures during the 30 min blooming period than did the medium and high pH samples. The low pH sample bloomed more rapidly and to a greater extent than medium and high pH samples. Among the 3 muscles, Longissimus had the highest, while Semimembranosus had the lowest a^* value and hue angle changes. Overall, refrigerated storage of vacuum packaged pork for 7 d or more resulted in more efficient blooming when pH was low. The ability of vacuum-packaged pork to rebloom is necessary for the meat to regain the consumer-expected pink-red color after opening the vacuum bag.     

Quality changes in beef complexus, serratus ventralis, vastus lateralis, vastus medialis, and longissimus dorsi muscles enhanced prior to aging

ABSTRACT:  Beef complexus (chuck), serratus ventralis (chuck), vastus lateralis (round), vatsus medialis (round), and longissimus dorsi (loin) muscles were enhanced (0.3% salt, 0.4% phosphate), vacuum packaged, and aged for 7 or 14 d. Enhancement increased positive sensory attributes (tenderness, juiciness, beef flavor, saltiness) and decreased negative attributes (off-flavors) of all muscles but had greater effects on some than on others. It increased the oily mouthfeel (10%) and saltiness (40%) and decreased the L * value and chroma of the complexus (chuck). It increased tenderness, juiciness, and beef flavor and decreased off-flavors, visual red and brown colors, shear value, and cook loss of the serratus ventrali (chuck) , vastus lateralis (round), vastus medialis (round), and longissiumus dorsi (loin). Aging slightly decreased rancid and liver off-flavor scores, visual brown color, and shear value and increased the hue angle and L * and b * values.     

EFFECT OF HOT WATER IMMERSION ON PEEL COLOR OF PEARS AND ON WHITE ROT CAUSED BY BOTRYOSPHAERIA BERENGERIANA

Abstract The effectiveness of hot water immersion (HWI) on peel color and the control of white rot caused by Botryosphaeria berengeriana on wound-inoculated European pears, cv. La-France, were investigated. After HWI at 40, 45, 48, 50, 52 and 54C for varying immersion periods, the pears were held at 20C, ≥ 90% RH (simulated retail conditions). The peel color (L*, a*, b*) was determined before and after HWI treatment. The dominant role of L* suggested that peel darkening was the major factor in color change of the HWI treated pears. The L* value of 54 was judged as the border line for acceptability and less than 51 resulted in browning. Similarly the critical points for a*, b*, chroma, hue and ΔE* were found to be −6, 34, 35, 100, and 8, respectively. Disease incidence recorded 7 days after treatment revealed that High Temperature Short Time (HTST) treatment of 54C for 3 min reduced incidence about 85% with good retention of color. Both temperature and immersion period are to be carefully selected to prolong shelf-life and retain the peel color quality of 'La-France'pears.     

Effect of carbon monoxide in modified atmosphere packaging, storage time and endpoint cooking temperature on the internal color of enhanced pork

The objective of this research was to evaluate the effects of gas atmosphere, refrigerated storage time, and endpoint temperature on internal cooked color of injection-enhanced pork chops. Enhanced chops were packaged in 0.36% CO/20.34% CO₂ (CO-MAP), 80% O₂/20% CO₂ (HO-MAP), or PVC-overwrapped (PVC-OW; controls), stored at 4 °C for 0, 12, 19 or 26 days, displayed for 2 days then cooked to six endpoint temperatures (54, 60, 63, 71, 77, and 82 °C). L^*, a^*, and b^* values, hue angle and chroma were determined on the internal cut surface of cooked chops. Chops packaged in CO-MAP had the highest a^* values; a^* value began increasing on day 14. The lowest hue angles occurred in chops cooked to lower endpoint temperatures. Chops in CO-MAP had lower hue angles and higher chroma than those in HO-MAP and PVC-OW. Above 71 °C, hue angle and chroma increased. Overall, CO-MAP packaged chops stored for longer time periods then cooked to lower endpoint temperatures appeared reddest. HO-MAP packaged chops were less red, did not change over time, and appeared more well done at lower endpoint temperatures than those in other gas atmospheres. CO-MAP packaged chops retained redness even after cooking at 82 °C.     

Measurement of meat color using a computer vision system

The limits of the colorimeter and a technique of image analysis in evaluating the color of beef, pork, and chicken were investigated. The Minolta CR-400 colorimeter and a computer vision system (CVS) were employed to measure colorimetric characteristics. To evaluate the chromatic fidelity of the image of the sample displayed on the monitor, a similarity test was carried out using a trained panel. The panelists found the digital images of the samples visualized on the monitor very similar to the actual ones (P < 0.001). During the first similarity test the panelists observed at the same time both the actual meat sample and the sample image on the monitor in order to evaluate the similarity between them (test A). Moreover, the panelists were asked to evaluate the similarity between two colors, both generated by the software Adobe Photoshop CS3 one using the L*, a* and b* values read by the colorimeter and the other obtained using the CVS (test B); which of the two colors was more similar to the sample visualized on the monitor was also assessed (test C). The panelists found the digital images very similar to the actual samples (P < 0.001). As to the similarity (test B) between the CVS- and colorimeter-based colors the panelists found significant differences between them (P < 0.001). Test C showed that the color of the sample on the monitor was more similar to the CVS generated color than to the colorimeter generated color. The differences between the values of the L*, a*, b*, hue angle and chroma obtained with the CVS and the colorimeter were statistically significant (P < 0.05–0.001). These results showed that the colorimeter did not generate coordinates corresponding to the true color of meat. Instead, the CVS method seemed to give valid measurements that reproduced a color very similar to the real one.     

Discoloration of Fresh Pork as Related to Muscle and Display Conditions

Pale, soft, exudative (PSE), normal, and dark, firm, dry (DFD) pork was held 7 days at 4°C under light or dark then color was evaluated instrumentally and visually. Change in reflectance (R630-R580) was used to estimate color changes due to oxymyoglobin. PSE pork was lighter (high L*) than normal and DFD pork, but did not change over time. An increase in a* and R630-R580 occurred the first day for normal and DFD, but not for PSE samples. A subsequent increase in hue angle and decrease in R630-R580 (loss of redness) occurred in PSE and normal, but not in DFD samples. Changes in hue angle and R630-R580 were more rapid in PSE than in normal samples. Light exposure accelerated all changes except L*. Visual redness inversely correlated with L* value.     

TOCOPHEROL EFFECTS ON FROZEN GROUND PORK COLOR

To assess the effects of α-tocopherol addition on lipid and pigment oxidation of ground pork, 10, 100 or 1000 ppm was added directly in a food grade carrier (2% medium chain triglyceride oil) then pork was overwrapped with polyvinyl-chloride (PVC) film and frozen (-34C) for seven months. Storage time increased extracted deoxymyoglobin percentage, metmyoglobin percentage determined by reflectance spectrophotometry, TBARS, hue angle, and pH, and decreased extracted oxymyoglobin percentage, L*, a* and b* values, and instrumental red color (% reflectance at 630 nm - % reflectance at 580 nm). Extracted metmyoglobin percentage increased through 140 days then decreased through 196 days. Alpha tocopherol increased deoxymyoglobin and decreased oxymyoglobin (extracted) expressed as a percentage of the total pigment, when compared with control samples. Metmyoglobin percentage (reflectance was highest in 10 ppm samples and lowest in 1000 ppm samples. Hue angle and total color were best correlated with other measures of red color.     

不同清洗处理对鲜切莲藕贮藏期褐变的影响

研究弱酸电解水、强酸电解水、次氯酸钠、去离子水4 种不同清洗处理对鲜切莲藕在4 ℃条件下贮藏14 d期间,色差值（L*、a*、b*,ΔE*及色相角）、褐变度、多酚氧化酶（PPO）活性及整体感观质量的变化。结果表明：弱酸电解水（pH 5.65,有效氯剂量 48 mL/L,氧化还原电位800 mV）能够有效保持鲜切莲藕的色泽品质和整体感观质量值,同时抑制褐变,降低PPO活性,有效地改善鲜切莲藕的贮藏品质,延长货架期。     

Determination of color, pigment, and phenolic stability in yogurt systems colored with nonacylated anthocyanins from Berberis boliviana L. as compared to other natural/synthetic colorants

ABSTRACT:  Anthocyanins are of interest to the food industry because of their antioxidant power, attractive color, and stability in high acid foods. Powder from the Peruvian berry Berberis boliviana Lechler, rich in nonacylated anthocyanins (7% to 8% dry weight), was incorporated into yogurt samples containing 3 different fat levels. Color (CIE L , a , b , chroma, and hue angle), pigment (monomeric anthocyanin and polymeric color), and total phenolics were monitored over 8 wk of storage and compared to yogurt treatments containing purple carrot acylated anthocyanins, red beet betalaines, or FD&C Red nr 40. Anthocyanin profiles were analyzed by HPLC coupled to photodiode array and mass detectors. Color of yogurt containing B. boliviana anthocyanins at 20 mg cyanidin-3-glucoside (cy-3-glu) equivalents/100 g yogurt ( L *= 65, chroma = 14, and hue angle = 335°) was similar to commercial blueberry yogurt ( L *= 65, chroma = 10.5, and hue angle = 341°). High color, pigment, and phenolic stability were observed in yogurts colored with B. boliviana , independent of the fat matrix. Acylated anthocyanins from purple carrot extracts exhibited increased stability with higher fat content. Anthocyanin degradation followed 1st-order kinetics. Pigment half-lives were 125 and 104 d for nonacylated anthocyanins at 10 and 20 mg cy-3-glu equivalents/100 g yogurt and 550.2, 232.6, and 128.9 d for acylated anthocyanins at 20 mg of cy-3-glu equivalents/100 g of 4%, 2%, and 0% fat yogurt. Addition of B. boliviana whole berry powder to yogurt matrices produced an attractive, stable anthocyanin-rich product, eliminating the need for industrial colorant extraction.     

SODIUM LACTATE/SODIUM POLYPHOSPHATE EFFECTS ON OXIDATION IN PRECOOKED FROZEN PORK PATTIES

The objective of this study was to evaluate sodium lactate (SL; 0, 1, 2 or 3%) and sodium polyphosphate (SP; 0, 0.1, 0.2 or 0.3%) effects on lipid oxidation and color characteristics of precooked pork patties during frozen storage. Ground pork was mixed with SL and/or SP, stuffed, frozen, sliced and cooked from the frozen state, packaged and held frozen for 14 weeks. Samples containing SP had lower TBARS, regardless of SL content. SL decreased pH, a* and b* values and red color (R630–R580). L* value, hue angle, pork flavor, saltiness, and juiciness increased as SL increased. A moderate correlation occurred between SL and TBARS. SP decreased hue angle, cook loss, and rancid flavor and increased pH, b* value, pork flavor, saltiness, and juiciness. Storage time decreased a* value, red color and juiciness, while saltiness, alkalinity, and rancid flavor scores increased. Based on both physical and sensory characteristics, optimum combinations appear to be 3% SL and 0.2–0.3% SP.     

Initial chilling rate of pre-rigor beef muscles as an indicator of colour of thawed meat

The effect of three rigor temperatures and two muscles of different fibre composition on the colour of thawed meat were determined. Within 45 min post-mortem, m. semitendinosus (ST) and m. biceps femoris (BF) from unstimulated heifer sides were held at 0, 10 and 35°C until they entered rigor. The rate of pH fall was higher at 35°C than at 0 and 10°C; and the pH values were lower (first 12 h post mortem) in the ST than the BF. Hunter L*, a* and b* and the hue angle increased with increasing rigor temperature (P<0.001). The ST was lighter and yellower and had greater hue angle than the BF. Colour parameters were plotted against the rate of muscle cooling in the first hour post mortem and linear regression lines were fitted to the data and equations were derived that gave a good indication of the colour and colour stability of the muscles as meat.