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1.
针对单通道振动信号的多特征分离问题,提出了一种基于正交非负矩阵分解的故障特征提取方法。首先,采用短时傅里叶变换,利用时频分布来描述信号中的局部故障特征,通过核心一致性指标评估子空间维数;然后,在幅值谱矩阵分解的基础上,通过正交性约束实现低维嵌入分量信息的分离,获取局部特征的准确描述;最后,采用相位恢复理论重构出特征波形,对仿真信号和滚动轴承故障数据进行了测试。结果表明,所提出的方法能利用单通道信号有效地分离出微弱的局部故障特征,为机械状态的早期故障诊断识别提供了一种有效手段。 相似文献
2.
LU YANG YUN LIU BOKE ZHANG MENGSI YU FEN HUANG YANG WEN JIANGZHENG ZENG YANDA LU CHANGCHENG YANG 《Biocell》2022,46(5):1215-1243
The aim of this study was to reveal genes associated with breast cancer metastasis, to investigate their intrinsic relationship with immune cell infiltration in the tumor microenvironment, and to screen for prognostic biomarkers. Gene expression data of breast cancer patients and their metastases were downloaded from the GEO, TCGA database. R language package was used to screen for differentially expressed genes, enrichment analysis of genes, PPI network construction, and also to elucidate key genes for diagnostic and prognostic survival. Spearman’s r correlation was used to analyze the correlation between key genes and infiltrating immune cells. We screened 25 hub genes, FN1, CLEC5A, ATP8B4, TLR7, LY86, PTGER3 and other genes were differentially expressed in cancer and paraneoplastic tissues. However, patients with higher expression of CD1C, IL-18 breast cancer had a better prognosis in the 10 years survival period, while patients with high expression of FN1, EIF4EBP1 tumors had a worse prognosis. In addition, TP53 and HIF1 genes are closely related to the signaling pathway of breast cancer metastasis. In this study, gene expression of ATP8B4 and CD1C were correlated with cancer tissue infiltration of CD8+ T lymphocytes, while GSE43816, GSE62327 and TCGA databases showed that CD8+ T lymphocytes were closely associated with breast cancer progression. Functional enrichment analysis of genes based on expression differences yielded key genes of prognostic value in the breast cancer microenvironment. 相似文献
3.
GIUSEPPE STEFANO NETTI FEDERICA SPADACCINO VALERIA CATALANO GIUSEPPE CASTELLANO GIOVANNI STALLONE ELENA RANIERI 《Biocell》2022,46(10):2235-2239
Pentraxin-3 (PTX3), the prototype of long pentraxins, seems to influence complement system (CS) modulation.PTX3 and CS sustain carcinogenesis, enriching tumor microenvironment (TME) with pro-inflammatory moleculespromoting angiogenesis in prostate cancer (PC) and renal cell carcinoma (RCC). Furthermore, cancer cellsoverexpress complement regulatory proteins, such as CD46, CD55 and CD59, which negatively affect complementpathways for support cancer cells survival. This viewpoint aims to elucidate the ambivalent role of PTX3 and the CSin the context of tumor microenvironment (TME). 相似文献
4.
Esophageal cancer (EC) was an aggressive malignant neoplasm characterized by high morbidity and poor prognosis. Identifying the changes in DNA damage repair genes helps to better understand the mechanisms of carcinoma progression. In this study, by comparing EC samples and normal samples, we found a total of 132 DDR expression with a significant difference. Moreover, we revealed higher expression of POLN, PALB2, ATM, PER1, TOP3B and lower expression of HMGB1, UBE2B were correlated to longer OS in EC. In addition, a prognostic risk score based on 7 DDR gene expression (POLN, HMGB1, TOP3B, PER1, UBE2B, ATM, PALB2) was constructed for the prognosis of EC. Meanwhile, EC cancer samples were divided into 3 subtypes based on 132 DDR genes expressions. Clinical profile analysis showed cluster C1 and C2 showed a similar frequency of T2, which was remarked higher than that in cluster 3. Moreover, we found the immune cell inflation levels were significantly changed in different subtypes of EC. The infiltration levels of T cell CD8+, B cell and NK cells were greatly higher in cluster 2 than that in cluster 1 and cluster 3. The results showed T cell CD4+ infiltration levels were dramatically higher in cluster 1 than that in cluster 2 and cluster 3. Finally, we perform bioinformatics analysis of DEGs among 3 subtypes of EC and found DDR genes may be related to multiple signaling, such as Base excision repair, Cell cycle, Hedgehog signaling pathway, and Glycolysis/Gluconeogenesis. These results showed DDR genes may serve as new target for the prognosis of EC and prediction of the potential response of immune therapy in EC. 相似文献
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Breast cancer is one of the most common cancers in the world and seriously threatens the health of women worldwide. Prognostic models based on immune-related genes help to improve the prognosis prediction and clinical treatment of breast cancer patients. In the study, we used weighted gene co-expression network analysis to construct a co-expression network to screen out highly prognostic immune-related genes. Subsequently, the prognostic immune-related gene signature was successfully constructed from highly immune-related genes through COX regression and LASSO COX analysis. Survival analysis and time receiver operating characteristic curves indicate that the prognostic signature has strong predictive performance. And we developed a nomogram by combing the risk score with multiple clinical characteristics. CIBERSORT and TIMER algorithms confirmed that there are significant differences in tumor-infiltrating immune cells in different risk groups. In addition, gene set enrichment analysis shows 6 pathways that differ between high- and low-risk group. The immune-related gene signature effectively predicts the survival and immune infiltration of breast cancer patients and is expected to provide more effective immunotherapy targets for the prognosis prediction of breast cancer. 相似文献
6.
YI WANG SHANGQI CHEN PENGCHENG YU ZHEHAN BAO CAN HU YUHANG XIA RUOLAN ZHANG LI YUAN HUA RUAN JIANCHENG SUN 《Biocell》2023,47(5):991-1002
Aim: Scavenger receptor class B, type I (SR-BI) is an integral plasma membrane protein that has been reported to be overexpressed in various malignancies, such as renal cancer, breast cancer, and prostate cancer, and is an independent prognostic factor. However, the clinical value and expression of SR-BI in GC are unknown. Our research aimed to explore the role of SR-BI in combination with immune markers as a diagnostic and prognostic marker for gastric cancer (GC). Methods: GC tissues, paracancerous tissues, and clinicopathological data of 149 patients were collected. The expression level of SR-BI, Tumor-infiltrating lymphocytes (TILs), and PD-L1 were evaluated by immunohistochemistry (IHC). The associations of the SR-BI staining intensity with clinicopathological features and immune markers were determined by the chi-square test. Univariate and multivariate COX regression analyses were used to evaluate independent prognostic factors. Kaplan–Meier analyses were performed to plot the survival curve. Results: Our results indicated that SR-BI was expressed at higher levels in tumor tissues than in adjacent paracancerous tissues (p < 0.001), and patients with high levels of SR-BI expression had a worse prognosis. Univariate and multivariate analyses revealed that high SR-BI expression was an independent factor for poor prognosis. The chi-square test determined that the expression of SR-BI was negatively correlated with CD4+ T cells and CD8+ T cells (CD4+ T cells, p = 0.013; CD8+ T cells, p = 0.021), and positively correlated with PD-L1 (p = 0.022). Finally, survival analysis revealed that CD4+ T cells were associated with the prognosis of GC patients (p = 0.019), and the combined survival analysis of SR-BI and CD4+ T cells was also statistically significant (p = 0.030). Conclusion: SR-BI is highly expressed in GC tissue and associated with poor prognosis. Moreover, SR-BI can also regulate the GC tumor immune microenvironment. 相似文献
7.
ATP binding cassette subfamily C member 8 (ABCC8) encodes a protein regulating the ATP-sensitivepotassium channel. Whether the level of ABCC8 mRNA in lower grade glioma (LGG) correlates with immune cellinfiltration and patient outcomes has not been evaluated until now. Comparisons of ABCC8 expression betweendifferent tumors and normal tissues were evaluated by exploring publicly available datasets. The association betweenABCC8 and tumor immune cell infiltration, diverse gene mutation characteristics, tumor mutation burden (TMB),and survival in LGG was also investigated in several independent datasets. Pathway enrichment analysis wasconducted to search for ABCC8-associated signaling pathways. Through an online database, we found that ABCC8expression in LGG was lower than in normal tissues. Then, the association of ABCC8 expression and immune cellinfiltration in LGG was discussed. As we expected, the ABCC8 mRNA levels were negatively associated with non-Timmune cell infiltration levels in all datasets. Consistently, TCGA_LGG RNA-seq data revealed that ABCC8downregulated several non-T immune cell-associated signaling pathways in gene set enrichment analysis. DifferentABCC8 expression groups showed diverse gene mutation characteristics and TMB. The high expression of ABCC8was linked to improved survival of LGG patients. A pathway enrichment analysis of ABCC8-associated genesindicated that the GABAergic synapse signaling pathway might be involved in regulating immunity in LGG. Ourfindings show that ABCC8 reflects LGG tumor immunity and is an ideal prognostic biomarker for LGG. 相似文献
8.
Purpose: Iron metabolism maintains the balance between iron absorption and excretion. Abnormal ironmetabolism can cause numerous diseases, including tumor. This study determined the iron metabolism-related genes(IMRGs) signature that can predict the prognosis of acute myeloid leukemia (AML). The roles of these genes in theimmune microenvironment were also explored. Methods: A total of 514 IMRGs were downloaded from theMolecular Characteristics Database (MSigDB). IMRGs related to AML prognosis were identified using Cox regressionand LASSO analyses and were used to construct the risk score model. AML patients were stratified into high-riskgroups (cluster 1) and low-risk groups (cluster 2) based on the mean value of the risk score. The accuracy andprognosis prediction potential of the risk-score model was evaluated using Kaplan-Meier and receiver operatingcharacteristics analysis. The stromal score, immune scores, and immune cells infiltrated in AML samples wereestimated using CIBERSORT, MCPcountre, and Xcell algorithms. The role of immune checkpoint genes in the AMLmicroenvironment and the prognostic value of the IMRGs were also evaluated. Results: An AML prognosisprediction model was established based on the eight most critical IMRGs. Further analyses revealed that the modelcould accurately predict AML prognosis. The expression of IMRGs correlated with the infiltration of several immunecells and could influence response to certain chemotherapy drugs and immunotherapy. Conclusion: A model basedon IMRGs can accurately predict the overall survival and disease-free survival of AML patients. 相似文献
9.
经直肠实时组织超声弹性成像在前列腺癌筛查中的应用 总被引:2,自引:0,他引:2
朱正 《现代仪器使用与维修》2013,(6):24-26
目的探讨经直肠实时组织超声弹性成像(TRTE)在前列腺癌筛查中的应用价值。方法对2010年1月~2012年12月我院93例疑似前列腺癌拟行前列腺活检的患者,在穿刺活检前首先分别行TRTE和经直肠超声(TRUS)检查前列腺,再在其引导下穿刺活检,比较2种方法的前列腺癌检出率。结果 93例前列腺癌疑似患者病理检查结果显示:良性45例,恶性48例。TRTE诊断前列腺癌灵敏度93.8%(45/48),特异度88.9%(40/45),准确度91.4%(85/93),阳性预测值90%(45/50),阴性预测值93%(40/43),明显优于TRUS检查(灵敏度77.1%,特异度37.8%,准确度58.1%,阳性预测值67.3%,阴性预测值44.7%,2种方法比较差异有统计学意义(P〈0.05)。结论 TRTE具有无创、经济、简单的优点,可提高前列腺癌的检出率,对于前列腺癌的早期筛查具有较高临床价值。 相似文献
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《Measurement》2016
Feature selection has been attracting more attentions in recent years for its advantages in improving the fault diagnosis efficiency and reducing the cost of feature acquisition. In this paper, we regard the feature selection as a clustering process with data decomposition technique and propose a novel feature selection method based on the non-negation matrix factorization (NMF). Alternating Least Squares (ALS) algorithm with sparsity control and decorrelation constrains is adopted to factorize original feature space into two low-rank matrixes (projection vectors and feature spaces). Considering the clustering distribution of the projection space, the optimal feature vectors are calculated by the means of the best updating rule parameters. Besides, the inverse of feature vectors is furtherly utilized in the seeking feature subset, which ensures high classifying performance. Experiments are performed by using two standard data sets and the fault diagnosis of roller bearing case. The results are compared with those obtained by applying the whole feature set and standard feature selection algorithms. The outcomes of comparative analysis have confirmed the effectiveness of the proposed approach. 相似文献
12.
Due to the non-stationary characteristics of vibration signals acquired from rolling element bearing fault, the time-frequency analysis is often applied to describe the local information of these unstable signals smartly. However, it is difficult to classify the high dimensional feature matrix directly because of too large dimensions for many classifiers. This paper combines the concepts of time-frequency distribution(TFD) with non-negative matrix factorization(NMF), and proposes a novel TFD matrix factorization method to enhance representation and identification of bearing fault. Throughout this method, the TFD of a vibration signal is firstly accomplished to describe the localized faults with short-time Fourier transform(STFT). Then, the supervised NMF mapping is adopted to extract the fault features from TFD. Meanwhile, the fault samples can be clustered and recognized automatically by using the clustering property of NMF. The proposed method takes advantages of the NMF in the parts-based representation and the adaptive clustering. The localized fault features of interest can be extracted as well. To evaluate the performance of the proposed method, the 9 kinds of the bearing fault on a test bench is performed. The proposed method can effectively identify the fault severity and different fault types. Moreover, in comparison with the artificial neural network(ANN), NMF yields 99.3% mean accuracy which is much superior to ANN. This research presents a simple and practical resolution for the fault diagnosis problem of rolling element bearing in high dimensional feature space. 相似文献
13.
《Measurement》2016
In this paper, a new dimensionality reduction based temperature distribution sensing (TDS) method is proposed to reconstruct the temperature distribution via the limited number of the scattered temperature measurement data. The projective nonnegative matrix factorization (PNMF) method is developed to exact the basis vectors, and the augmented Lagrangian multipliers (ALM) method is proposed to solve the proposed PNMF model. A dimensionality reduction model is obtained via projecting the original temperature distribution onto the spaces spanned by a set of basis. An objective functional that considers the inaccurate properties of the reconstruction model and the measurement data, the Shearlet regularization and the total variation (TV) method is proposed to convert the TDS task into an optimization problem, where the temperature distribution is indirectly reconstructed via solving a low-dimensional vector. An iteration scheme is developed to solve the objective functional. Numerical simulation results validate the feasibility of the proposed reconstruction algorithm. 相似文献
14.
《Measurement》2014
In the analysis towards the energy dispersive X-ray diffraction (EDXRD) spectra of drug and explosive concealed by body packing, positive matrix factorization (PMF) was introduced to extract features from EDXRD spectra of samples in a set of drugs and explosive concealed in the anthropomorphic phantom, because PMF prevents the negative factors from occurring, avoids contradicting physical reality, and makes factors more easily interpretable. In order to compare with the features extracted by PMF, Principal Component Analysis (PCA) and robust PCA were investigated. Then, K-nearest neighbour (KNN) and support vector machine (SVM) were introduced to classify the samples according to the features extracted by PMF, PCA and robust PCA. It is shown that the recognition rates obtained by PMF are highest (above 99.5%) and insensitive to classifiers. This work demonstrates that PMF is effective in feature extraction for identification of drug and explosive concealed by body packing. 相似文献
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非负矩阵分解(NMF)作为一种矩阵分解以及非线性维数约简工具,被广泛用于多样本振动时频谱的分解编码以及特征提取,但单样本振动时频谱的NMF编码、尤其NMF分解向量与振动时频谱分量间关联关系尚缺乏探讨。阐述了单时频谱编码与解调的特征提取原理,重点分析了NMF对单时频谱基于部分的特征表示能力、单时频谱NMF基向量的带通滤波幅频特性(BFAC)、以及NMF编码向量与时频谱分量的同步变化特性。提出单时频谱NMF编码与解调的两种特征提取新方法,即基于NMF基向量的滤波解调和NMF编码向量直接解调,定义一种BFAC指数指标和基向量归一化的NMF编码优化迭代规则分别用于NMF低维参数自适应选取和优化求解过程。将所提方法用于仿真信号以及齿轮箱振动信号分析,6.4 k长度数据在给定因子分解秩和NMF最大迭代300次终止条件设定下的特征提取用时约3.5 s,同时实现了对信噪比为-10 dB仿真信号以及多故障齿轮箱振动信号中故障特征的提取。 相似文献
17.
Jing LIU;Kangxin LI;You ZHANG;Yi LIU 《光学精密工程》2022,30(14):1657-1668
To solve the nonlinear unmixing problem of hyperspectral remote sensing images, a multi-graph regularized multi-kernel nonnegative matrix factorization (MGMKNMF) method is proposed, and the multi-graph regularization term in multi-kernel space is constructed. Moreover, the MGMKNMF objective function, which includes multi-graph in multi-kernel regularization, multi-kernel weights regularization, and multi-graph weights regularization terms, is constructed. MGMKNMF can update the multi-kernel and multi-graph weights during the process of learning endmembers and abundance, and precisely construct the graph of the input data in the appropriate multi-kernel space, thereby solving the problem of selecting the graph and kernel weights. Compared with the single kernel function used in kernel nonnegative matrix factorization (KNMF), multiple kernel functions can determine a more appropriate kernel space. Further, compared with the single graph in graph regularized nonnegative matrix factorization (GNMF), multiple graphs describe the relationship between samples in different ways, which is more accurate and reliable than the single graph. The experimental results with two real measured datasets and two simulated datasets show that the presented MGMKNMF algorithm is effective. Compared with GNMF, non-pure pixels kernel nonnegative matrix factorization kernel sparse non-negative matrix factorization, kernel-based nonlinear spectral unmixing with dictionary pruning methods, and multi-graph regularized kernel nonnegative matrix factorization method, the average spectral angle distance (SAD) values of the proposed MGMKNMF are the best, that is, 0.092 1 and 0.097 0 on the real Cuprite dataset and Jasper ridge dataset, respectively. The average SAD values of MGMKNMF on the Hapke and generalized bilinear model simulated datasets are 0.137 5 and 0.145 6, respectively. Finally, the root mean square error values are 0.050 6 and 0.057 0, respectively. 相似文献
18.
针对转子异常振动产生含交叉频率的响应,其共频相关故障源不满足统计独立要求,提出利用非负矩阵法在频域中计算故障源个数,不计及源信号和混合系统特性,可以正确估计出故障源数目或源数上限。提出利用小波包分解故障信号,选择互信息较小的子带进行重构,剔除共频信号并进行盲分离,得到独立非相关的源信号,保留了故障信息。理论及实验结果证明了所提出方法的有效性。 相似文献
19.
Mitotic kinesin KIFC1 plays critical roles in mitosis by regulating the spindle length, pole formation, and known for clustering extra centrosomes in cancer cells. Centrosome clustering is associated with the survival of cancer cells, but this phenomenon remains obscure in prostate cancer (PCa). The present study demonstrated that PCa cells showed centrosome amplification and clustering during interphase and mitosis, respectively. KIFC1 is highly expressed in PCa cells and tumor tissues of prostatic adenocarcinoma (PAC) patients. Up-regulation of KIFC1 facilitated the PCa cell survival in vitro by ensuring bipolar mitosis through clustering the multiple centrosomes, suggesting centrosome clustering could be a leading cause of prostate carcinogenesis. Conversely, the silencing of KIFC1 resulted in normal centrosome number or multipolar mitosis by inhibiting the clustering of amplified centrosomes in PCa cells. Besides, knockdown of KIFC1 by RNAi in PCa cells reduced cancer cell survival, and proliferation. KIFC1 interacted with centrosome structural protein Centrin 2 in clustering of amplified centrosomes in PCa cells to ensure the bipolar mitotic spindle formation. Knockdown of Centrin 2 in PCa cells inhibited the centrosome amplification and clustering. Moreover, up-regulated KIFC1 promotes PCa cell proliferation via progression of cell cycle possibly through aberrant activation of cyclin dependent kinase 1(Cdk1). Therefore, KIFC1 can be a prognostic marker and therapeutic target of PCa for inhibiting the cancer cell proliferation. 相似文献
20.
基于非负矩阵分解的盲信号源数估计 总被引:1,自引:0,他引:1
为满足盲源分离算法对振源信号数量的苛刻要求,提出了一种基于非负矩阵分解的源数估计方法。该方法在传感器数大于或等于源数时,无论源信号是否相关均能准确估计源数;在传感器数小于源数时,能估计源数的下界。理论分析、仿真和工程实验证明了该方法的有效性和可行性。 相似文献