共查询到20条相似文献,搜索用时 31 毫秒
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纤维素燃料乙醇已成为下一代燃料乙醇的必然发展方向。文章综述了近年来以木质纤维素为原料生产燃料乙醇的关键技术,重点对物理法、化学法、蒸汽爆破法、生物法等木质纤维素原料预处理技术,酸水解、酶水解等水解(糖化)技术,以及直接发酵法、水解发酵两步法、同步水解发酵法等发酵工艺进行了总结,并指出了未来纤维素乙醇的产业化过程中必须解决的关键问题和发展趋势。 相似文献
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寻找化石能源的替代品以及开发和利用生物能源已引起国内外研究者的广泛关注。提高酿酒酵母利用来源广泛、贮存丰富的农林废弃物等木质纤维素原料生产燃料乙醇的效率是生物能源的重要研究内容,但是,重组酿酒酵母木糖发酵性能低是限制纤维素乙醇经济性的关键问题。本文总结了酿酒酵母中木糖代谢途径的构建和优化以及木糖转运对木糖利用的影响,分析了重组酵母利用纤维素水解液进行乙醇发酵的研究现状,并对进一步提高重组酿酒酵母纤维素乙醇生产效率的研究趋势进行了展望。目前国内外已经构建了可有效利用木糖产乙醇的重组酵母,但对其木糖代谢机制的研究还尚未深入,限制了重组菌株的定向改造。此外,目前缺少在纤维素生物质水解液发酵实际应用过程中对重组菌株的评价。因此,加强重组酵母菌株对木糖利用相关代谢调控机理的分析,注重多种抑制物对菌株发酵性能的影响,结合真实底物纤维素乙醇发酵过程进行重组菌株的构建和优化,从而进一步提高纤维素乙醇生产的经济性,是未来菌株构建的重要研究方向。 相似文献
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Jari Heinonen Anu Tamminen Jaana Uusitalo Tuomo Sainio 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2012,87(5):689-696
BACKGROUND: Production of bioethanol from wood using concentrated acid hydrolysis has received less attention than the dilute acid hydrolysis route. The feasibility of producing lignocellulosic bioethanol from spruce and birch via concentrated acid hydrolysis was studied experimentally. Hydrolysis with sulfuric acid, chromatographic purification of the hydrolysate, and fermentation of the monosaccharides were investigated. RESULTS: Monosaccharide yields of 70% were obtained in the hydrolysis of spruce and birch. Only low amounts of by‐products were formed. With chromatographic purification of the hydrolysate, over 90% of the hydrolysis acid was recovered for recycling, and furfural and HMF were removed completely. Most of the acetic acid was recovered in a separate fraction. The monosaccharide yield in a single pass separation was approximately 70%. In the fermentation, S. cerevisiae produced higher amounts of ethanol and more efficiently than P. stipitis. Chromatographically purified hydrolysates gave higher ethanol productivities and yields than Ca(OH)2 neutralized hydrolysates. CONCLUSIONS: Chromatographic purification of concentrated acid lignocellulosic hydrolysates has advantages when compared with neutralization with Ca(OH)2. With chromatography, most of the inhibitory compounds can be removed from the hydrolysates. In addition, due to the recycling of the hydrolysis acid, the economy of the bioethanol manufacturing process is increased considerably. Copyright © 2011 Society of Chemical Industry 相似文献
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联合生物加工产纤维素乙醇中真菌的开发与应用 总被引:1,自引:0,他引:1
联合生物加工(consolidated bioprocessing,CBP)是在单一或组合微生物作用下,将纤维素酶生产、纤维素水解糖化、戊糖和己糖发酵产醇整合于单一步骤的生物加工过程。本文从真菌在CBP产纤维素乙醇中的开发历程着眼,回顾了纤维素乙醇产业化的发展进程,介绍了CBP产纤维素乙醇的作用机理,系统总结了目前国内外文献中报道的CBP底盘真菌的主要种类及优缺点,并综述了CBP真菌的开发策略,包括工程化策略和共培养策略,着重阐述了工程化策略的技术路线和研究进展。指出综合运用先进生物技术和基于代谢分析数据的计算机模拟系统开发CBP目标微生物,设计新型高效的生物反应器以及将CBP技术与现有生物工业整合,是未来将CBP技术应用于纤维素乙醇产业的关键。 相似文献
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竹材制取生物乙醇原料预处理技术研究进展 总被引:2,自引:0,他引:2
竹子具有可再生性强、生长周期短且富含纤维素、半纤维素,是生产乙醇的重要潜在原料之一。目前有关木质纤维素乙醇的研究主要围绕原料预处理、酶解、发酵三大关键步骤进行,其中原料预处理的能耗和效率问题是该工艺的重要制约因素。本文在综述国内外木质纤维素乙醇原料预处理的基础上,着重分析了竹材的化学组成和结构以及各种竹材预处理的优缺点。包括机械粉碎法能耗大,蒸汽爆破法对设备的要求高,化学方法易造成环境污染,生物方法生产周期长、效率低,离子液体优点明显但需要更深入的研究。提出采用不同预处理工艺联合使用,以期达到优势互补的目的。 相似文献
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Razif Harun Michael K. Danquah Gareth M. Forde 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2010,85(2):199-203
BACKGROUND: The increasing cost of fossil fuels as well as the escalating social and industrial awareness of the environmental impacts associated with the use of fossil fuels has created the need for more sustainable fuel options. Bioethanol, produced from renewable biomass such as sugar and starch materials, is believed to be one of these options, and it is currently being harnessed extensively. However, the utilization of sugar and starch materials as feedstocks for bioethanol production creates a major competition with the food market in terms of land for cultivation, and this makes bioethanol from these sources economically less attractive. RESULT: This study explores the suitability of microalgae (Chlorococum sp.) as a substrate for bioethanol production via yeast (Saccharomyces bayanus) under different fermentation conditions. Results show a maximum ethanol concentration of 3.83 g L?1 obtained from 10 g L?1 of lipid‐extracted microalgae debris. CONCLUSION: This productivity level (~38% w/w), which is in keeping with that of current production systems endorses microalgae as a promising substrate for bioethanol production. Copyright © 2009 Society of Chemical Industry 相似文献
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Hao Fang Yuntao Deng Yingjie Pan Chaofeng Li Liang Yu 《American Institute of Chemical Engineers》2022,68(11):e17844
The consolidated bioprocessing (CBP) of lignocellulose by the synthetic microbial consortium of Trichoderma reesei and Saccharomyces cerevisiae is a promising way of biomanufacturing d -glucaric acid. However, the hindrance factor to its industrial application is the low efficiency. Therefore, we engineered T. reesei and S. cerevisiae to improve the CBP for d -glucaric acid production. T. reesei was engineered to produce more cellulase and release more fermentable sugars from lignocellulose, that is, pushing more sugars to S. cerevisiae. S. cerevisiae was engineered to metabolize cellobiose and siphon more sugars into d -glucaric acid biosynthetic pathway, that is, pulling more sugars to S. cerevisiae. This is the strategy of distributive and collaborative push-and-pull we developed and proposed in this work, which was proven successful in improving efficiencies of the CBPs of steam-exploded corn stover (SECS) for d -glucaric acid production and distiller's grains for single cell protein (SCP) production. The titer, yield and productivity of d -glucaric acid produced from 50 g/L SECS by the microbial consortium of T. reesei C10 and S. cerevisiae LGA-1C3S2 were 6.42 g/L, 0.128 g/g SECS, and 0.917 g/L/d, respectively. The titer, yield, and productivity of SCP produced from 80 g/L distiller's grains were 50.5 g/L, 0.631 g/g distiller's grains, and 8.417 g/L/d, respectively. These were much higher than the initial microbial consortia of T. reesei Rut-C30 and S. cerevisiae LGA-1 or INVSc1. The results confirmed the applicability and generalizability of distributive and collaborative push-and-pull, which has profound meaning for science and engineering. 相似文献
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花生壳发酵生产生物乙醇可行性研究 总被引:1,自引:0,他引:1
研究以花生壳为原料,采用超临界水一步法获得可发酵糖液,并通过混合菌种将可发酵糖液转化为生物乙醇的工艺方法。不仅使农业废弃物得到综合利用,同时,也获得了良好的经济效益和环境效益。 相似文献
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纤维素稀酸水解产物中发酵抑制物的去除方法 总被引:4,自引:0,他引:4
在纤维素稀酸水解发酵制乙醇的过程中,由于弱酸、呋喃衍生物和苯系化合物对微生物的影响,乙醇的产量和产率都不高。针对国内对这些抑制物质的去除研究较少的现状,重点介绍了国外去除纤维素稀酸水解产物中发酵抑制物的各种方法,这些方法能有效地降低各种抑制物质的影响,从而能够获得更高的乙醇产量和产率。 相似文献