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1.
Lih-Ling Wang Andrew C. Peng Andrew Proctor 《Journal of the American Oil Chemists' Society》1990,67(8):499-502
Lipids from five cultivars of highbush blueberries (Vaccinium corymbosum L.) were extracted and fractionated into neutral lipids (60–66%), glycolipids (20–22%) and phospholipids (14–18%). The major
fatty acids in all fractions were palmitic (16∶0), oleic (18∶1), linoleic (18∶2), and linolenic (18∶3) acids. All lipid classes
had a large concentration of C18 polyunsaturated acids (84–92%), indicating that blueberries are a rich source of linoleic and linolenic acids. Changes in
the fatty acid composition of neutral lipids and phospholipids were not significantly different among the five cultivars,
but significant differences were noted in the ratios of linoleic and linolenic acids in the glycolipids fraction. 相似文献
2.
The lipid composition of Yoshida ascites hepatoma cells was analyzed together with that of ascitic plasma and of livers and
blood plasma from host and normal rats. In comparison to normal livers, host livers showed no significant differences in the
content of the various lipid classes, but contained a higher percentage of palmitic acid and a lower proportion of arachidonic
acid in the major phospholipid classes. In addition, tumor growth induced a marked hypertriglyceridemia in host animals; changes
in the concentration of other plasma lipid classes were not statistically significant. The ascitic plasma contained small
amounts of lipids mainly constituted by cholesteryl esters and phospholipids. Yoshida hepatoma cells contained less phospholipids
in comparison to both host and normal liver, while the increased level of triglycerides and the decrease of free fatty acids
were not statistically significant. Hepatoma cells showed appreciable amounts of ether-linked lipids associated in part to
neutral lipids (as glyceryl ether diesters) and, in part, to ethanolamine and choline phosphoglycerides. The alkyl groups
in GEDE as well as in ethanolamine and choline phosphoglycerides were mainly constituted by C16∶0 and C18∶0 followed by C18∶1. The alk-1-enyl groups in ethanolamine and choline phosphoglycerides were C16∶0 and C18∶0 with only a minor proportion of C18∶1. In comparison to both host and normal liver, Yoshida hepatoma cells showed significant changes in the fatty acid composition
of neutral lipids and phospholipids. Some of the major changes consisted of an increase of monoenoic acids associated with
a decrease of arachidonic and docosahexaenoic acids in phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol. 相似文献
3.
Silicic acid column chromatography was used to separate the polar lipids of goats' milk into glycolipid, phosphatidylethanolamine,
phosphatidylserine plus phosphatidylinositol, phosphatidylcholine, and sphingomyelin fractions. Each fraction was purified
by column chromatography and its fatty acid profile determined by gas liquid chromatography and mass spectrometry. The glycerophospholipids
each contained 18∶1 as the predominant fatty acid (∼45%). The sphingolipids contained a high percentage of long-chain saturated
fatty acids (C22 to C24>45%); the glycolipid fraction also contained ca. 2% 2-hydroxy fatty acids. The data represent a comprehensive cross-sectional
study of the major polar lipids found in goats' milks. 相似文献
4.
Turkey hens were fed diets containing no added fat nor diets supplemented with soybean oil or neatsfoot oil. The composition
of neutral and polar lipid fatty acids present in the unincubated turkey egg yolk was compared with that of those present
in the yolk sac of the developing turkey embryo at different stages of development. Comparisons were made of the fatty acid
fractions in the entire embryo homogenates, except liver and heart, which were analyzed separately. Changes in the relative
amounts of the fatty acids are reported as affected by age of the embryo and by dietary lipids. The fatty acids from both
the neutral and polar lipids which were utilized to the greatest extent for embryonic development were palmitoleic, oleic,
linoleic, and linolenic, regardless of the dietary supplements. Arachidonic, tetracosenoic, and docosahexaenoic acids also
were metabolized by the embryo. Saturated fatty acids, used by the embryo as development progressed, were palmitic, stearic,
and arachidic acids. Analyses of the liver fatty acids showed that the C16∶0 C16∶1, C18∶0, C18∶1, and C20∶4 acids in the neutral
and polar lipids decreased with embryonic development and varied with the type of diet. The heart contained low levels of
myristic, palmitic, stearic, arachidic, and arachidonic acids in the neutral lipids and palmitoleic and oleic acids in the
polar lipids. 相似文献
5.
Two unusual lipid classes were detected by thin-layer chromatography in the neutral lipids derived from goat cauda-epididymal
sperm plasma membrane. The lipids were identified as wax esters and 1-O-alkyl-2,3-diacylglycerols based on chromatographic properties, identity of their hydrolysis products, and infrared/1H nuclear magnetic resonance spectral evidence. The membrane containedca. 3 and 5 μg/mg protein of wax esters and alkyldiacylglycerols, respectively. The relative proportions of wax esters and alkyldiacylglycerols
in the total neutral lipids were 1.5% and 2.4%, respectively. The lipids contained fatty acids with chain lengths of C14 to C22. The major fatty acids of the wax esters were 14∶0, 16∶0, 16∶1ω7, 18∶0 and 18∶1ω9. The fatty acids in alkyldiacylglycerol
were 16∶0, 18∶0, 22∶5ω3 and 22∶6ω3. Alkyldiacylglycerol was particularly rich in docosahexaenoic acid 22∶6ω3) representing
30% of the total fatty acids. The alcohols of wax ester were all saturated with C20–C29 carbon chains. The deacylated products derived from alkyldiacylglycerols were identified as hexadecyl, octadecyl and octadec-9′-enyl
glycerol ethers. 相似文献
6.
The carbon and energy source for aerobically grown cultures ofCandida guilliermondii profoundly influenced the neutral lipid content and the fatty acid composition of the individual lipid components. Methanol
(0.80%, w/v) grown cells cultivated at 30 C in presence of 0.025% ammonium sulfate contained 12% total lipids, 67% of which
was neutral lipids. Glucose (0.74%, w/v) or ethanol (0.53%, w/v) grown cells contained 21–22% total lipids, 80% of which was
neutral lipids, under the same conditions. Methanol-grown cells contained a decreased 18∶1 acid (52–54% of total fatty acids)
and an increased 18∶2 acid (23–25%), as compared with glucose- or ethanol-grown cells which contained 57–66% 18∶1 acid and
8–14% 18∶2 acid, in both neutral and polar lipid fractions. The relationship between methanol metabolism and desaturation
of fatty acid in yeast was discussed. 相似文献
7.
The neutral and polar lipid composition ofEntomophthora coronata was determined qualitatively. The fungus was grown on a chemically nondefined medium (Sabouraud dextrose yeast extract) and
a chemically defined medium for a period up to 26 days. The lipids were characterized by thin-layer, column, gas chromatography,
and selective sprays,32P-labeling, and mass spectrometry. The neutral lipids consist of monoglycerides, diglycerides, cholesterol, free fatty acids,
triglycerides, and cholesteryl esters. The polar lipids consist of phospholipids (phosphatidyl ethanolamine, phosphatidyl
choline, lysophosphatidyl ethanolamine, lysophosphatidyl choline, and spingomyelin), a number of glycolipids including cerebrosides,
and many unrecognizable lipids, most of which are present in trace amounts. The cerebrosides and spingomyelin are present
in significant amounts, and their concentration increased with age of the culture. The major fatty acids (>10%) of the total,
neutral, and polar lipids of the mycelia are 14∶0, 16∶0, 18∶1, 18∶3(γ), and 24∶1. The polar lipids of total culture (unsaturation
index 0.88) and of the conidia (unsaturation index 1.48) are considerably more unsaturated than the corresponding neutral
lipids (unsaturated index 0.50 and 0.49). The mycelial polar lipids, compared to the neutral lipids, possess less 14∶0 and
18∶1 but contain a greater percentage of 16∶0, 18∶2, 18∶3(γ), 24∶0, and 24∶1. The major fatty acid of the conidia (>10%) are
13∶0, 14∶0, 18∶1, 18∶2, 18∶3(γ), and 20∶4. Their polar lipids have a higher proportion of 18∶2, 18∶3(γ), and 20∶4. The cerebrosides
possess 24.1 in high relative proportion (30.1%).
Presented at the AOCS Meeting, Atlantic City, October 1971. 相似文献
8.
The lipid class compositions of adult Pacific oysters [Crassostrea gigas (Thunberg)] were examined using latroscan thin-layer chromatography/flame-ionization detection (TLC/FID), and fatty acid
compositions determined by capillary gas chromatography and gas chromatography/mass spectrometry (GC/MS). The fatty acid methyl
esters were separated using argentation TLC and also analyzed as their 4,4-dimethyloxazoline derivatives using GC/MS. Major
esterified fatty acids inC. gigas were 16∶0, 20∶5n−3, and 22∶6n−3. C20 and C22 nonmethylene interrupted (NMI) fatty acids comprised 4.5 to 5.9% of the total fatty acids. The NMI trienoic fatty acid 22∶3(7,13,16) was also identified. Very little difference was found in the proportions of the various lipid classes, fatty acids or sterols
between samples of adult oysters of two different sizes. However, significant differences in some of the lipid components
were evident according to the method of sample preparation used prior to lipid extraction with solvents. Lyophilization (freeze
drying) of samples led to a significant reduction in the amounts of triacylglycerols (TG) extracted by solvents in two separate
experiments (7.0 and 52.5% extracted). Extracts from lyophilized samples had less 16∶0, C18 unsaturated fatty acids, and 24-ethylcholest-5-en-3β-ol, while C20 and C22 unsaturated fatty acids comprised a higher proportion of the total fatty acids. There was no significant change in the amounts
of polar lipids, total sterols, free fatty acids or hydrocarbons observed in extracts from lyophilized samples relative to
extracts from nonlyophilized samples. Addition of water to the freezedried samples prior to lipid extraction greatly improved
lipid yields and resulted in most of the TG being extracted. 相似文献
9.
Lipids of some thermophilic fungi 总被引:1,自引:0,他引:1
Total lipid content in the thermophilic fungi—Thermoascus aurantiacus, Humicola lanuginosa, Malbranchea pulchella var.sulfurea, andAbsidia ramosa—varied from 5.3 to 19.1% of mycelial dry weight. The neutral and polar lipid fractions accounted for 56.4 to 80.2% and 19.8
to 43.6%, respectively. All the fungi contained monoglycerides, diglycerides, triglycerides, free fatty acids, and sterols
in variable amounts. Sterol ester was detected only inA. ramosa. Phosphatide composition was: phosphatidyl choline (15.9–47%), phosphatidyl ethanolamine (23.4–67%), phosphatidyl serine
(9.3–17.6%), and phosphatidyl inositol (1.9–11.9%). Diphosphatidyl glycerol occurred in considerable quantity only inH. lanuginosa andM. pulchella var.sulfurea. Phosphatidic acid, detected as a minor component only inM. pulchella var.sulfurea andA. ramosa, does not appear to be a characteristic phosphatide of thermophilic fungi as suggested earlier. The 16∶0, 16∶1, 18∶0, 18∶1,
and 18∶2 acids were the main fatty acid components. In addition,A. ramosa contained 18∶3 acid. Total lipids contained an average of 0.93 double bonds per mole of fatty acids, and neutral lipids tend
to be more unsaturated than phospholipids. 相似文献
10.
Robert S. Burkhalter Carol A. Smith David C. White Ronald Fayer Andrew B. White 《Lipids》1998,33(8):829-833
Heating or freezing leads to loss in infectivity of oocysts of Cryptosporidium parvum toward neonatal BALB/c mice and is reflected in the profile of the polar lipid fatty acids. Upon loss of infectivity, the
ratio of polar lipid to neutral lipid fatty acid decreased and the relative proportions of 18∶1n-9 also decreased; proportions
of 18∶2n-6 and 20∶5n-6 increased, whereas the proportions of 16∶0 remained constant with freezing. During these investigations,
a novel fatty acid, 10-OH 18∶0, was discovered in the glycolipid fraction. The identification of a fatty acid unique to species
of Cryptosporidium was thought to provide a specific biomarker for this organism. Cryptosporidium also demonstrated fluctuations in absolute quantities of 10-OH 18∶0 with events that lead to loss of infectivity. This led
to the presumed correlation of this biomarker with infectious Cryptosporidium. The 10-OH 18∶0 was putatively localized at the sn-2 position of phosphatidylethanolamine. High-performance liquid chromatography/electrospray ionization mass spectrometry
revealed that the 10-OH 18∶0 existed principally in the free fatty acid form. Herein, we establish that the free fatty acid
10-OH 18∶0 was, in actuality, an artifact of the procedures for sample preparation. 相似文献
11.
Shahina Zaka Bushra Asghar M. Y. Raie S. A. Khan M. K. Bhatty 《European Journal of Lipid Science and Technology》1989,91(5):205-207
The purified crude lipid of Psoralia corylifolia seeds was subjected to lipid class and fatty acid analysis by thin layer and gas chromatography. The lipid classes identified were triacyl glycerol, free fatty acid, diacyl glycerol, mono acyl glycerol, hydrocarbon-waxester and polar lipid fractions. Most of the fractions were found to contain high level of C18:1 while C18:0, C18:3 and C20:0 were also found to be present in all the lipid fractions. It has been observed that the diacyl and monoacyl glycerol fractions contain significant amounts of C14:0 and C18:0 while the hydrocarbon-waxester fraction was rich in C22:0. The polar lipids contain high level of C18:3 and low level of C18:1 as compared to other lipid fractions. The fatty acid composition of the whole oil was also determined and found to be similar to other fractions. Unidentified long chain fatty acids were also present in significant amounts in all the lipid fractions. 相似文献
12.
Yu-Yan Yeh 《Lipids》1988,23(12):1114-1118
A restricted maternal dietary intake (40% of ad libitum intake) is known to cause myelin deficit that is accompanied by decreased
amounts of individual phospholipids and sphingolipids in brain myelin of suckling rats. This communication reports the effects
of the same nutritional stress on the fatty acid composition of brain myelin lipids. In myelin of 19-day-old normally fed
rats, palmitate (16∶0), stearate (18∶0) and oleate (18∶1) accounted for 80–90% of all fatty acids in phosphatidylcholine,
phosphatidylethanolamine and phosphatidylserine. Maternal dietary restriction resulted in deficits of total fatty acid content,
but did not affect the proportional distribution of individual fatty acids among phospholipids. By contrast, longer chain
(22- and 24-carbon) fatty acids accounted for more than half the fatty acid content of myelin cerebroside and sulfatide from
the 19-day-old control rat pups. In undernourished rats of that age, proportions of lignocerate (24∶0) and nervonate (24∶1)
in cerebroside and sulfatide were 40–50% lower than those in control rats. This, together with higher proportions of 16∶0,
18∶0 and 18∶1 and a higher ratio of C16−C20 to C22−C24 in under-nourished than in control rats, suggests an impairment in fatty acid chain elongation. Ten days of nutritional rehabilitation
failed to restore the fatty acid imbalances; however, after an additional 5 days of ad libitum feeding, the experimental and
control values were similar. The undernutrition results in hypomyelination, which is characterized by a proportional decrease
in lignoceric and nervonic acids of sphingolipids. 相似文献
13.
Pigeon “milk” (PM) collected from the crop of 1- to 5-day-old squabs was analyzed to examine whether there were changes in
lipid composition during the first week of secretion. The high PM fat content (9–11%) remained fairly constant in the first
5 days of secretion. The mean percentage of neutral lipids, glycolipids and phospholipids was 80, 12 and 8%, respectively.
Unlike the content of neutral lipids, glycolipid and phospholipid levels increased significantly between day 1 and day 5 of
secretion. Triglycerides, the major neutral lipids, decreased by 24% between day 1 and day 5, while free sterols, monoglycerides
and hydrocarbons increased by 8%, 11% and 2.5%, respectively, during the same period; diglycerides and sterol esters, however,
remained unchanged. The ratio of saturated to unsaturated fatty acids was 0.27 and it remained unchanged. Medium-chain (C10, C12 and C14) and oddchain (C15 and C17) fatty acid contents were low. Fatty acids longer than C20 were absent. Palmitic acid, the major saturated fatty acid, increased by 42% from day 1 to day 5, whereas stearic acid decreased
by 48% during the same period. Oleic acid, the predominant unsaturated fatty acid, also decreased from 51 to 45% between the
first and fifth day of PM secretion. Polyunsaturated acids (18∶2, 18∶3 and 20∶4) accounted for 26% and 30% of the total fatty
acids on day 1 and day 5, respectively. Although lipid changes in the crop of squabs prior to collection of samples cannot
totally be ruled out, the nature of lipid changes is likely to reflect cellular breakdown that precedes PM secretion by parent
pigeons. 相似文献
14.
Nikolaus Weber Klaus Vosmann Kurt Aitzetmüller Christin Filipponi Horst Taraschewski 《Lipids》1994,29(6):421-427
The sterol composition of free sterol and steryl ester fractions of the fish parasiteParatenuisentis ambiguus was determined. In addition, the fatty acid composition of various neutral lipid classes, i.e., wax esters, steryl esters,
triacylglycerols and free fatty acids, as well as the composition of the 1-O-alkyl moieties of total ether glycerolipids of the parasite, were investigated. The results of these studies were compared
with those obtained on the intestinal tract tissue of its host, the eel (Anguilla anguilla). Cholesterol is the major sterol in bothP. ambiguus andA. anguilla. However, the sterols ofP. ambiguus contain high proportions (>20%) of other sterols, such as campesterol and various dehydrosterols. [e.g., 7-dehydrocholesterol
and cholesta-5,22(E)-dienol]. The presence of these minor sterols agrees with the known biotransformations of exogenous sterols in various helminths.
Considerable differences are found in the fatty acid composition of neutral lipid fractions, as well as the total lipid extract
from the endoparasite as compared to the host tissue. In particular, eicosapentaenoic acid (20∶5n−3), other polyunsaturated
fatty acids, such as 20∶4n−6, 22∶5n−3 and 22∶6n−3, as well as long-chain saturated fatty acids, such as 20∶0, are generally
enriched in the neutral lipid fractions of the parasite as compared to those of infected eel intestine. The analysis of ether
glycerolipids revealed that 1-O-hexadecyl (16∶0) and 1-O-hexadecenyl (16∶1) moieties were present in similar proportions in the ether lipids of bothP. ambiguus and eel intestine, whereas 1-O-octadecyl (18∶0) moieties are more prominent in the parasite and 1-O-octadecenyl (18∶1) moieties in the eel. The results of these studies show thatP. ambiguus has specific mechanisms for the regulation of the sterol and fatty acid composition of its neutral lipids.
Dedicated to Professor Helmut K. Mangold on the occasion of his 70th birthday. 相似文献
15.
The lipid composition of six thermophilic fungi (Myriococcum albomyces, Mucor miehei, Papulaspora thermophila, Rhizopus sp.,Thielavia thermophila (+)Thielavia thermophila (−), andTorula thermophila) was examined. The relative per cent total lipids (4.9–26.3%), neutral lipids (55.5–88.3%), polar lipids (11.7–44.6%) and
the fatty acid profile of each lipid fraction was determined. The predominant fatty acids were 16∶0, 18∶0 and 18∶2, and lesser
amounts of 12∶0, 14∶0, 15∶0, 16∶1, 16∶2, 17∶0 and 18∶3 were present. The total lipids contained an average of 0.96 double
bonds per mole fatty acid (unsaturation index [USI]) the neutral lipids 0.86 USI and the polar lipids 0.84 USI, excluding
the values forTorula thermophila. These data show a high degree of saturation and are consistent with data reported for other fungal thermophiles.Torula thermophila possessed abnormally high USI values (1.15–1.50) and was cultured at three different temperatures (25, 45 and 51 C). As the
culture temperature ofTorula thermophila increased, the USI decreased. The USI of the polar lipids ofTorula thermophila at 25, 45 and 51 C were 1.50, 1.28 and 1.11, respectively. Thus the membrane lipids of this fungus appear unusual for a thermophile. 相似文献
16.
Five ganglioside fractions from bovine adrenal medulla were analyzed with respect to their fatty acid and long chain base
compositions. The five fractions included two hematosides and three hexasamine-containing species, the latter having chromatographic
properties comparable to the major gangliosides of brain. The fatty acid compositions of all five were similar: 22∶0 was the
most abundant, but significant amounts of 16∶0, 18∶0, 24∶0 and 24∶1 were also present. No hydroxy fatty acids were detected.
The principal long chain base in each fraction was 4-sphingenine (sphingosine), with lesser amounts of the C16 and C17 homologues. Minor quantities of the corresponding saturated bases were also detected. These were identified by two gas liquid
chromatography methods: (a) trimethylsilyl ether derivatives, (b) aldehydes formed by periodate oxidation of the long chain
bases. No 4-eicosasphingenine (C20-sphingosine), characteristic of brain gangliosides, was found in any of the fractions. The results demonstrate that gangliosides
of the adrenal medulla show tissue specificity in fatty acid and long chain base composition which is independent of carbohydrate
structure. 相似文献
17.
Fatty acids ofSterculia foetida were added to the medium used to maintain the Morris hepatoma 7288C in culture. The effect of this supplement on the lipid
composition was examined. Overall, monoene levels were decreased with 18∶1 levels reduced by 40%. Saturated fatty acid levels
were increased, with stearate (18∶0) levels 220% of control values. No effect occurred on the level of polyunsaturates (18∶2,
20∶4, 22∶5, 22∶6). These changes in fatty acid makeup were observed in both neutral and phospholipid fractions, and all lipid
classes were affected. Triglycerides were most affected with a 66% decrease in 18∶1. There appeared to be little specificity
of effect in the phospholipids with 18∶1 levels decreased 40–60% in all classes. All classes were therefore dependent on an
endogenous supply of 18∶1. Examination of the distribution of geometrical isomers of 18∶1 reveals that in all lipid classes,
except diphosphatidylglycerol (DPG), the ratio of Δ11 to Δ9 isomer decreased toward the isomeric distribution displayed by
total medium lipids. In DPG, although 18∶1 levels were lowered, the isomeric distribution increased. DPG, synthesized and
found in the mitochondria, may use a separate pool of 18∶1 during synthesis. Cyclopropene fatty acids (sterculic and malvalic)
were incorporated into both neutral and phospholipid fractions with preferential incorporation into triglycerides. Cyclopropene
fatty acids were not selectively incorporated into any phospholipid species. Sphingomyelin did not incorporate cyclopropene
fatty acids, indicating that a different class of acyltransferase is used in the formation of this phospholipid class. 相似文献
18.
Comparative studies on the fatty acid composition of moderately and extremely thermophilic bacteria 总被引:11,自引:0,他引:11
The fatty acids of three strains of extremely thermophilic bacteria and three strains of moderately thermophilic bacteria
were examined by gas liquid chromatography. All the thermophiles contained straight, iso, and ante-iso branched fatty acids.
Iso C17∶0 acid was abundant in both the moderately thermophilic strains (10–33%) and the extremely thermophilic strains (50–61%). The
pair of fatty acids iso C15∶0 and iso C17∶0 was the predominant pair in both the moderately (34–64%) and extremely (76–87%) thermophilic strains. The pair of fatty acids
ante-iso C15∶0 and ante-iso C17∶0 was present in larger amount in moderately (25–34%) than in extremely (8.5–15%) thermophilic strains. No hydroxy cyclopropane,
or unsaturated fatty acids were found. One extreme thermophile,Flavobacterium thermophilum HB-8 was grown at 6 different culture temperatures from 49–82 C, and the changes of its fatty acid composition were studied.
The ratios of iso C17∶0/iso C15∶0 and ante-iso C17∶0/ante-iso C15∶0 were much greater at higher culture temperatures, indicating chain elongation. 相似文献
19.
In this study we determined in rats the complete 24-h lymphatic fatty acid profile after administration of either rapeseed
oil (RO) or rapeseed oil interesterified with 10∶0 (RO/C10) with special emphasis on the transition from absorptive to postabsorptive phase. Rats were subjected to cannulation of the
main mesenteric lymph duct and the next day oils were administered through a gastric feeding tube. Lymph was collected in
1-h fractions for the following 24 h. The time for maximum lymphatic transport of fatty acids was at 4 h with fast changes
in fatty acid composition from the fatty acids of endogenous origin to those of the administered oils. Seven to eight hours
after administration the transport was significantly lower than maximum, indicating the change from absorptive to postabsorptive
phase. At 24 h after administration of either oil the transport of total fatty acids, palmitic acid (16∶0), and linoleic acid
(18∶2n−6) together with oleic acid (18∶1n−9) after RO had not returned to the transport at baseline. In contrast, the transport
of decanoic acid (10∶0) and α-linolenic acid (18∶3n−3) returned to baseline values between 12 and 15 h. This indicated that
the absorption of purely exogenous fatty acids (illustrated by 10∶0 and 18∶3n−3) was complete at 15 h and that the fatty acids
transported between 15 and 24 h were derived mostly from endogenous stores. 相似文献
20.
Six strains ofListeria monocytogenes belonging to four different serotypes all had similar fatty acid profiles when grown at 37 C, with C15 and C17 branched chain acids as major components. The proportion of 17∶0 br decreased markedly as the growth temperature was lowered
from 37 C to 4 C, and a reduction of 18∶1 with increasing age of cultures was observed in cells harvested at different stages
of the growth curve. The fatty acid composition was also affected by the nature of the culture medium. Two other genera of
the family Corynebacteriaceae were analyzed for fatty acid composition. Strains ofErysipelothrix rhusiopathiae isolated from human, turkey, dog and pig had rather similar patterns, consisting mainly of straight chain, even-numbered
fatty acids from C10 to C18. The three species ofCorynebacterum analyzed each had quite different fatty acid patterns.C. poinsettiae bore some resemblance toL. monocytogenes butC. pseudodiphtheriticum had much higher proportions of 16∶0 and 18∶1 andC. equi contained a rather complex mixture of fatty acids.
Part of this work was carried out in the Collip Medical Research Laboratory. 相似文献