液相色谱二极管阵列荧光法测定羊绒制品中的3种酚类激素

建立了羊绒制品中双酚A、4-n-壬基酚和辛基酚3种酚类环境激素的高效液相色谱二极管阵列荧光检测方法.样品经索氏提取后,在Shim-pack XR-ODSⅡ快速色谱柱上分离,采用甲醇/乙腈/水(体积比为30∶50∶20)为流动相,紫外检测波长为225 nm,荧光检测激发波长为228 nm,发射波长为305 nm,外标法定...     

调味品中罗丹明B的HPLC-FLD-DAD测定方法研究

建立了高效液相色谱(HPLC)-荧光检测器(FLD)-二极管阵列检测器(DAD)测定调味品中罗丹明B的方法.不同基质样品采用适宜的提取液提取后,以甲醇和0.02 mol/L的乙酸铵溶液(70 ∶ 30)为流动相,经C18色谱柱分离,用荧光-二极管阵列检测器串联测定,FLD激发波长为547 nm,发射波长为573 nm,DAD检测波长为550 nm,外标法定量.实验结果表明:罗丹明B用FLD检测在1～2000 ng/mL浓度范围内呈良好的线性关系,定量限为0.005 mg/kg;DAD检测在100～2000 ng/mL浓度范围内呈良好的线性关系,定量限为0.5 mg/kg.方法回收率在83.1％～105.1％之间,RSD为1.64％～5.42％(n=6).该方法准确、灵敏,前处理简单,适用于调味品中罗丹明B的定性、定量分析.     

高效液相色谱荧光法检测海产品中苯并(a)芘及其代谢产物

目的 建立检测海产品中苯并(a)芘(BaP)及其代谢产物3-羟基苯并芘(3-OHBaP)的高效液相色谱荧光法(HPLC-FD).方法 样品经65％乙腈提取,涡旋混匀,离心沉淀,分别以甲醇-水(pH 4.5)(85∶ 15)、(87∶ 13)为流动相进行色谱分离BaP、3-OHBaP,色谱柱为Century SIL C18-BDS(150 mm×4.6 mm,5μm),流速1.0 ml/min;荧光检测器检测BaP激发波长265 nm,发射波长450 nm,检测3-OHBaP激发波长365 nm,发射波长450 nm;进样量20μl.结果 BaP在0.1～10.0 ng/ml浓度范围内线性关系良好,相关系数r=0.999 4;3-OHBaP在1.07 ～100.7 ng/ml浓度范围内线性关系良好,相关系数r=0.992 1.BaP、3-OHBaP回收率分别在96.8％～ 112.0％、72.6％～ 83.3％之间;相对标准偏差分别为6.0％、5.2％(n=5);最低检测限分别为0.1和0.2μg/kg.结论 该法简便、快速、灵敏度高,可用于海产品中BaP及其代谢产物3-OHBaP的检测.     

高效液相色谱法测定啤酒中异α-酸含量

高效液相色谱法测定了7种啤酒样品中异α-酸的含量,实验研究了色谱流动相组成、比例及进样量、流速、柱温的色谱条件,得出高效液相色谱测定啤酒中异α-酸的最佳优化条件:流动相为A(1%H_3PO_4)∶B(ACN∶H_2O∶H_3PO_4=80∶19∶1)=10∶90;检测波长:249nm;流速:1.0mL/min;柱温:30℃:进样量:10μL,测定7种啤酒样品中的异α-酸含量,分别为:青岛啤酒(4.46μg/mL)、燕京啤酒(2.92μg/mL)、蓝带啤酒(1.34μg/mL)、雪花啤酒(0.8μg/mL)、哈尔滨啤酒(1.12μg/mL)、百威啤酒(1.06μg/mL)、崂山啤酒(6.39μg/mL)。本实验为啤酒行业评价不同品牌啤酒质量、人们选择喜爱的啤酒风味提供了一种简便快速的检测方法。     

高效液相色谱外标法测定荧光增白剂ER-1含量

建立了高效液相色谱外标法测定荧光增白剂ER-1{ 1,4’-双[2-(邻氰苯基)乙烯基]苯}含量的方法.采用Kromasil C18色谱柱,流动相为甲醇-水[V(甲醇):V(水)=90:10],紫外检测波长为365 nm,外标法定量.样品的平均加标回收率为99.52％,相对标准偏差(RSD)为0.31％,检出限(S/N=3)为0.01 μg/mL.该法简便准确、灵敏度高,能快速有效控制产品质量,对提高产品质量稳定性和竞争力有较好的应用价值.     

发酵行业4-乙烯基愈创木酚和4-乙基愈创木酚研究进展

介绍了4-乙烯基愈创木酚和4-乙基愈创木酚在发酵行业的研究进展,列举了一些有价值的研究实例,指出4-乙烯基愈创木酚和4.乙基愈创木酚是白酒、啤酒(特别是小麦啤酒)、葡萄酒、酱油等产品的重要呈香物质,并从原料、酵母菌种和工艺角度对它们形成的途径和影响因素进行了探讨,提出以分子生物学手段对酵母菌种进行基因克隆是全面提升传统发酵产品质量的关键.     

风味物质4-乙烯基愈创木酚的研究进展

4-乙烯基愈创木酚是一种挥发性酚类化合物,具有强烈香辛料、丁香和发酵香味,是决定白酒、啤酒、葡萄酒和酱油等产品的重要风味物质。该文综述了4-乙烯基愈创木酚的理化性质、生物合成及在发酵食品中的作用,介绍近年来4-乙烯基愈创木酚的研究状况,为提高发酵食品的质量奠定基础。     

高效液相色谱法测定酱香型白酒中挥发性酚类物质

利用酚类物质的荧光特性和手性拆分剂β-环糊精拆分重叠峰,建立带荧光检测-高效液相色谱仪法测定酱香型白酒中10种挥发性酚类物质含量的方法,色谱条件如下：色谱柱,Synergi 4u Hydro-RP 80A (150mm×4.6mm,4μm);荧光检测器波长：Ex=270nm,Em=315nm;流动相A：冰醋酸-β-环糊精的水溶液(1:100,V/V);流动相B：冰醋酸-乙腈-β-环糊精的水溶液(1:40:60,V/V),梯度洗脱分析。该法对10种挥发性酚类化合物测定的回收率为83.8%～101.6%,相对标准偏差为0.9%～2.5%,检出限为0.0025～0.0506μg/mL。说明该方法准确度、灵敏度和重复性好。     

皂化提取－高效液相色谱法测定油茶籽油中苯并(a)芘残留

建立了一种先皂化油茶籽油,经萃取和净化,高效液相色谱-荧光检测器测定油茶籽油中苯并(a)芘的检测方法.采用2 mol/L氢氧化钾乙醇溶液皂化,石油醚萃取,中性氧化铝净化柱净化后,再在下述条件下测定:多环芳烃(PAH)C18柱,流动相为体积比95∶5的乙腈-0.5％磷酸水溶液,流速1.0 mL/min,荧光激发波长384 nm,发射波长406 nm,柱温30℃.在2.00 ～ 20.0 μg/kg的添加水平,加标回收率在93.0％～110.0％之间,相对标准偏差在2.59％～9.57％,该方法的检出限为0.2 μg/kg.该方法对油茶籽油中苯并(a)芘提取完全,操作简单,重现性好,检测灵敏度高,对阳性样品判断更有优势.     

高效液相色谱法测定水产品中4-己基间苯二酚

建立了水产品中4-己基间苯二酚(4-HR)残留量测定的高效液相色谱-荧光法.样品经乙腈提取,浓缩后用流动相溶解残渣,正己烷脱脂净化,过滤后供高效液相色谱(HPLC)分析.色谱条件为:WondaSil C18柱(250mm×4.6mm,5μm);以甲醇-水(75:25,v/v)作为流动相,流速为1.0mL/min;荧光检测器激发波长为280nm,发射波长为310nm,外标法定量.结果表明:该方法在0.05 ～ 5.0μg/mL范围内线性关系良好,相关系数R2=0.9998.样品在0.1、0.2、1.0、5.0mg/kg加标水平时,平均回收率为90.9％ ～ 108.4％,相对标准偏差(RSD)为0.8％～8.1％(n=5).方法定量限(LOQ)为0.1mg/kg.     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the