共查询到20条相似文献,搜索用时 15 毫秒
1.
Song Zhang Xiangjian Chen Chunrong Gu Yu Zhang Jindan Xu Zhiping Bian Di Yang Ning Gu 《Nanoscale research letters》2009,4(1):70-77
Recently, magnetic nanoparticles of iron oxide (Fe3O4, γ-Fe2O3) have shown an increasing number of applications in the field of biomedicine, but some questions have been raised about the
potential impact of these nanoparticles on the environment and human health. In this work, the three types of magnetic nanoparticles
(DMSA-Fe2O3, APTS-Fe2O3, and GLU-Fe2O3) with the same crystal structure, magnetic properties, and size distribution was designed, prepared, and characterized by
transmission electronic microscopy, powder X-ray diffraction, zeta potential analyzer, vibrating sample magnetometer, and
Fourier transform Infrared spectroscopy. Then, we have investigated the effect of the three types of magnetic nanoparticles
(DMSA-Fe2O3, APTS-Fe2O3, and GLU-Fe2O3) on smooth muscle cells (SMCs). Cellular uptake of nanoparticles by SMC displays the dose, the incubation time and surface
property dependent patterns. Through the thin section TEM images, we observe that DMSA-Fe2O3 is incorporated into the lysosome of SMCs. The magnetic nanoparticles have no inflammation impact, but decrease the viability
of SMCs. The other questions about metabolism and other impacts will be the next subject of further studies.
Song Zhang and Xiangjian Chen contributed equally to this work. 相似文献
2.
Nea Repar Eva Jarc Jovi
i Ana Kump Giovanni Birarda Lisa Vaccari Andreja Erman Slavko Kralj Sebastjan Nemec Toni Petan Damjana Drobne 《International journal of molecular sciences》2022,23(13)
Superparamagnetic iron oxide nanoparticles (SPIONs) have great potential for use in medicine, but they may cause side effects due to oxidative stress. In our study, we investigated the effects of silica-coated SPIONs on endothelial cells and whether oleic acid (OA) can protect the cells from their harmful effects. We used viability assays, flow cytometry, infrared spectroscopy, fluorescence microscopy, and transmission electron microscopy. Our results show that silica-coated SPIONs are internalized by endothelial cells, where they increase the amount of reactive oxygen species (ROS) and cause cell death. Exposure to silica-coated SPIONs induced accumulation of lipid droplets (LD) that was not dependent on diacylglycerol acyltransferase (DGAT)-mediated LD biogenesis, suggesting that silica-coated SPIONs suppress LD degradation. Addition of exogenous OA promoted LD biogenesis and reduced SPION-dependent increases in oxidative stress and cell death. However, exogenous OA protected cells from SPION-induced cell damage even in the presence of DGAT inhibitors, implying that LDs are not required for the protective effect of exogenous OA. The molecular phenotype of the cells determined by Fourier transform infrared spectroscopy confirmed the destructive effect of silica-coated SPIONs and the ameliorative role of OA in the case of oxidative stress. Thus, exogenous OA protects endothelial cells from SPION-induced oxidative stress and cell death independent of its incorporation into triglycerides. 相似文献
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Greta Jarockyte Egle Daugelaite Marius Stasys Urte Statkute Vilius Poderys Ting-Chen Tseng Shan-Hui Hsu Vitalijus Karabanovas Ricardas Rotomskis 《International journal of molecular sciences》2016,17(8)
The uptake and distribution of negatively charged superparamagnetic iron oxide (Fe3O4) nanoparticles (SPIONs) in mouse embryonic fibroblasts NIH3T3, and magnetic resonance imaging (MRI) signal influenced by SPIONs injected into experimental animals, were visualized and investigated. Cellular uptake and distribution of the SPIONs in NIH3T3 after staining with Prussian Blue were investigated by a bright-field microscope equipped with digital color camera. SPIONs were localized in vesicles, mostly placed near the nucleus. Toxicity of SPION nanoparticles tested with cell viability assay (XTT) was estimated. The viability of NIH3T3 cells remains approximately 95% within 3–24 h of incubation, and only a slight decrease of viability was observed after 48 h of incubation. MRI studies on Wistar rats using a clinical 1.5 T MRI scanner were showing that SPIONs give a negative contrast in the MRI. The dynamic MRI measurements of the SPION clearance from the injection site shows that SPIONs slowly disappear from injection sites and only a low concentration of nanoparticles was completely eliminated within three weeks. No functionalized SPIONs accumulate in cells by endocytic mechanism, none accumulate in the nucleus, and none are toxic at a desirable concentration. Therefore, they could be used as a dual imaging agent: as contrast agents for MRI and for traditional optical biopsy by using Prussian Blue staining. 相似文献
6.
Ke Son Phan Prof. Phuong Thu Ha Dr. Huu Nghi Do Dr. Trung Anh Nguyen Dr. Thuc Quang Bui Dr. Hong Nam Pham Prof. Mai Huong Le Dr. Thi Thu Huong Le 《ChemMedChem》2021,16(23):3615-3625
The multifunctional nano drug delivery system (MNDDS) has much revolutionized in cancer treatment, aiming to eliminate many disadvantages of conventional formulations. This paper herein proposes and demonstrates MNDDS inspired by poly(lactide)-tocopheryl polyethylene glycol succinate (PLA-TPGS) copolymer co-loaded Doxorubicin and magnetic iron oxide nanoparticles (MIONs) with a 1 : 1 (w/w) optimal ratio. In vitro drug release kinetics of Doxorubicin from this nanosystem fitted best to the Weibull kinetic model and can be described by the classical Fickian diffusion mechanism under acidic pH conditions. The combination of MIONs and Doxorubicin in the PLA-TPGS copolymer has maintained the fluorescence properties of Doxorubicin and good cell penetration, especially inside the nucleus and its vicinity. Moreover, different cell cycle profiles were observed in HeLa cell lines treated with MNDDSs. 相似文献
7.
Thomas Schlorf Manuela Meincke Elke Kossel Claus-Christian Gl��er Olav Jansen Rolf Mentlein 《International journal of molecular sciences》2011,12(1):12-23
Superparamagnetic iron-oxide particles (SPIO) are used in different ways as contrast agents for magnetic resonance imaging (MRI): Particles with high nonspecific uptake are required for unspecific labeling of phagocytic cells whereas those that target specific molecules need to have very low unspecific cellular uptake. We compared iron-oxide particles with different core materials (magnetite, maghemite), different coatings (none, dextran, carboxydextran, polystyrene) and different hydrodynamic diameters (20–850 nm) for internalization kinetics, release of internalized particles, toxicity, localization of particles and ability to generate contrast in MRI. Particle uptake was investigated with U118 glioma cells und human umbilical vein endothelial cells (HUVEC), which exhibit different phagocytic properties. In both cell types, the contrast agents Resovist, B102, non-coated Fe3O4 particles and microspheres were better internalized than dextran-coated Nanomag particles. SPIO uptake into the cells increased with particle/iron concentrations. Maximum intracellular accumulation of iron particles was observed between 24 h to 36 h of exposure. Most particles were retained in the cells for at least two weeks, were deeply internalized, and only few remained adsorbed at the cell surface. Internalized particles clustered in the cytosol of the cells. Furthermore, all particles showed a low toxicity. By MRI, monolayers consisting of 5000 Resovist-labeled cells could easily be visualized. Thus, for unspecific cell labeling, Resovist and microspheres show the highest potential, whereas Nanomag particles are promising contrast agents for target-specific labeling. 相似文献
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氧化铁磁性纳米粒子的表面修饰及应用 总被引:1,自引:0,他引:1
氧化铁磁性纳米粒子因其优异的性能,广泛应用于环境分离、生物活性物质的富集和分离等领域,近年来引起了广泛的关注和研究。文章总结了氧化铁肱性纳米粒子表面修饰方法及相关应用,并对其前号进行了展望。 相似文献
10.
Ujwal S. Patil Shiva Adireddy Ashvin Jaiswal Sree Mandava Benjamin R. Lee Douglas B. Chrisey 《International journal of molecular sciences》2015,16(10):24417-24450
Increasing biomedical applications of iron oxide nanoparticles (IONPs) in academic and commercial settings have alarmed the scientific community about the safety and assessment of toxicity profiles of IONPs. The great amount of diversity found in the cytotoxic measurements of IONPs points toward the necessity of careful characterization and quantification of IONPs. The present document discusses the major developments related to in vitro and in vivo toxicity assessment of IONPs and its relationship with the physicochemical parameters of IONPs. Major discussion is included on the current spectrophotometric and imaging based techniques used for quantifying, and studying the clearance and biodistribution of IONPs. Several invasive and non-invasive quantification techniques along with the pitfalls are discussed in detail. Finally, critical guidelines are provided to optimize the design of IONPs to minimize the toxicity. 相似文献
11.
Chienwen Huang Jiechao Jiang Chivarat Muangphat Xiankai Sun Yaowu Hao 《Nanoscale research letters》2011,6(1):43
Synthesis of the core/shell-structured Fe3O4/Au nanoparticles by trapping Fe3O4 inside hollow Au nanoparticles is described. The produced composite nanoparticles are strongly magnetic with their surface plasmon resonance peaks in the near infrared region (wavelength from 700 to 800 nm), combining desirable magnetic and plasmonic properties into one nanoparticle. They are particularly suitable for in vivo diagnostic and therapeutic applications. The intact Au surface provides convenient anchorage sites for attachment of targeting molecules, and the particles can be activated by both near infrared lights and magnetic fields. As more and more hollow nanoparticles become available, this synthetic method would find general applications in the fabrication of core–shell multifunctional nanostructures. 相似文献
12.
Jan Zaloga Christina Janko Rohit Agarwal Johannes Nowak Robert Müller Aldo R. Boccaccini Geoffrey Lee Stefan Odenbach Stefan Lyer Christoph Alexiou 《International journal of molecular sciences》2015,16(5):9368-9384
Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted increasing attention in many biomedical fields. In magnetic drug targeting SPIONs are injected into a tumour supplying artery and accumulated inside the tumour with a magnet. The effectiveness of this therapy is thus dependent on magnetic properties, stability and biocompatibility of the particles. A good knowledge of the effect of storage conditions on those parameters is of utmost importance for the translation of the therapy concept into the clinic and for reproducibility in preclinical studies. Here, core shell SPIONs with a hybrid coating consisting of lauric acid and albumin were stored at different temperatures from 4 to 45 °C over twelve weeks and periodically tested for their physicochemical properties over time. Surprisingly, even at the highest storage temperature we did not observe denaturation of the protein or colloidal instability. However, the saturation magnetisation decreased by maximally 28.8% with clear correlation to time and storage temperature. Furthermore, the biocompatibility was clearly affected, as cellular uptake of the SPIONs into human T-lymphoma cells was crucially dependent on the storage conditions. Taken together, the results show that the particle properties undergo significant changes over time depending on the way they are stored. 相似文献
13.
Vasilisa V. Krasitskaya Alexander N. Kudryavtsev Roman N. Yaroslavtsev Dmitry A. Velikanov Oleg A. Bayukov Yulia V. Gerasimova Sergey V. Stolyar Ludmila A. Frank 《International journal of molecular sciences》2022,23(10)
Starch-coated magnetic iron oxide nanoparticles have been synthesized by a simple, fast, and cost-effective co-precipitation method with cornstarch as a stabilizing agent. The structural and magnetic characteristics of the synthesized material have been studied by transmission electron microscopy, Mössbauer spectroscopy, and vibrating sample magnetometry. The nature of bonds between ferrihydrite nanoparticles and a starch shell has been examined by Fourier transform infrared spectroscopy. The data on the magnetic response of the prepared composite particles have been obtained by magnetic measurements. The determined magnetic characteristics make the synthesized material a good candidate for use in magnetic separation. Starch-coated magnetic iron oxide nanoparticles have been tested as an affinity sorbent for one-step purification of several recombinant proteins (cardiac troponin I, survivin, and melanoma inhibitory activity protein) bearing the maltose-binding protein as an auxiliary fragment. It has been shown that, due to the highly specific binding of this fragment to the starch shell, the target fusion protein is selectively immobilized on magnetic nanoparticles and eluted with the maltose solution. The excellent efficiency of column-free purification, high binding capacity of the sorbent (100–500 µg of a recombinant protein per milligram of starch-coated magnetic iron oxide nanoparticles), and reusability of the obtained material have been demonstrated. 相似文献
14.
Chih-Jung Chen Hsin-Yi Lai Chee-Cheng Lin Jiun-Shen Wang Ray-Kuang Chiang 《Nanoscale research letters》2009,4(11):1343-1350
Iron fatty acid complexes (IFACs) are prepared via the dissolution of porous hematite powder in hot unsaturated fatty acid. The IFACs are then decomposed in five different organic solvents under reflux conditions in the presence of the respective fatty acid. The XRD analysis results indicate that the resulting NPs comprise a mixture of wustite, magnetite, and maghemite phases. The solvents with a higher boiling point prompt the formation of larger NPs containing wustite as the major component, while those with a lower boiling point produce smaller NPs with maghemite as the major component. In addition, it is shown that unstable NPs with a mixed wustite–magnetite composition can be oxidized to pure maghemite by extending the reaction time or using an oxidizing agent. 相似文献
15.
Kristin R. Di Bona Yaolin Xu Marquita Gray Douglas Fair Hunter Hayles Luckie Milad Alex Montes Jennifer Sherwood Yuping Bao Jane F. Rasco 《International journal of molecular sciences》2015,16(12):30251-30268
Iron oxide nanoparticles (NPs) are commonly utilized for biomedical, industrial, and commercial applications due to their unique properties and potential biocompatibility. However, little is known about how exposure to iron oxide NPs may affect susceptible populations such as pregnant women and developing fetuses. To examine the influence of NP surface-charge and dose on the developmental toxicity of iron oxide NPs, Crl:CD1(ICR) (CD-1) mice were exposed to a single, low (10 mg/kg) or high (100 mg/kg) dose of positively-charged polyethyleneimine-Fe2O3-NPs (PEI-NPs), or negatively-charged poly(acrylic acid)-Fe2O3-NPs (PAA-NPs) during critical windows of organogenesis (gestation day (GD) 8, 9, or 10). A low dose of NPs, regardless of charge, did not induce toxicity. However, a high exposure led to charge-dependent fetal loss as well as morphological alterations of the uteri (both charges) and testes (positive only) of surviving offspring. Positively-charged PEI-NPs given later in organogenesis resulted in a combination of short-term fetal loss (42%) and long-term alterations in reproduction, including increased fetal loss for second generation matings (mice exposed in utero). Alternatively, negatively-charged PAA-NPs induced fetal loss (22%) earlier in organogenesis to a lesser degree than PEI-NPs with only mild alterations in offspring uterine histology observed in the long-term. 相似文献
16.
David J. Burke Nicole Pietrasiak Shu F. Situ Eric C. Abenojar Mya Porche Pawel Kraj Yutthana Lakliang Anna Cristina S. Samia 《International journal of molecular sciences》2015,16(10):23630-23650
In this study, we investigated the effect of positively and negatively charged Fe3O4 and TiO2 nanoparticles (NPs) on the growth of soybean plants (Glycine max.) and their root associated soil microbes. Soybean plants were grown in a greenhouse for six weeks after application of different amounts of NPs, and plant growth and nutrient content were examined. Roots were analyzed for colonization by arbuscular mycorrhizal (AM) fungi and nodule-forming nitrogen fixing bacteria using DNA-based techniques. We found that plant growth was significantly lower with the application of TiO2 as compared to Fe3O4 NPs. The leaf carbon was also marginally significant lower in plants treated with TiO2 NPs; however, leaf phosphorus was reduced in plants treated with Fe3O4. We found no effects of NP type, concentration, or charge on the community structure of either rhizobia or AM fungi colonizing plant roots. However, the charge of the Fe3O4 NPs affected both colonization of the root system by rhizobia as well as leaf phosphorus content. Our results indicate that the type of NP can affect plant growth and nutrient content in an agriculturally important crop species, and that the charge of these particles influences the colonization of the root system by nitrogen-fixing bacteria. 相似文献
17.
Rafa Mazgaj Pawe Lipiski Mateusz Szudzik Aneta Joczy Zuzanna Kope Adrian M. Stankiewicz Marian Kamyczek Dorine Swinkels Beata elazowska Rafa R. Starzyski 《International journal of molecular sciences》2021,22(18)
Iron deficiency is the most common mammalian nutritional disorder. However, among mammalian species iron deficiency anemia (IDA), occurs regularly only in pigs. To cure IDA, piglets are routinely injected with high amounts of iron dextran (FeDex), which can lead to perturbations in iron homeostasis. Here, we evaluate the therapeutic efficacy of non-invasive supplementation with Sucrosomial iron (SI), a highly bioavailable iron supplement preventing IDA in humans and mice and various iron oxide nanoparticles (IONPs). Analysis of red blood cell indices and plasma iron parameters shows that not all iron preparations used in the study efficiently counteracted IDA comparable to FeDex-based supplementation. We found no signs of iron toxicity of any tested iron compounds, as evaluated based on the measurement of several toxicological markers that could indicate the occurrence of oxidative stress or inflammation. Neither SI nor IONPs increased hepcidin expression with alterations in ferroportin (FPN) protein level. Finally, the analysis of the piglet gut microbiota indicates the individual pattern of bacterial diversity across taxonomic levels, independent of the type of supplementation. In light of our results, SI but not IONPs used in the experiment emerges as a promising nutritional iron supplement, with a high potential to correct IDA in piglets. 相似文献
18.
本文探讨了将铁红试样用盐酸溶解后,在60~70℃温度条件下,用磺基水杨酸作指示剂,用抗坏血酸作标准滴定溶液,直接滴定出试液中的Fe^3 ;然后,再用双氧水将Fe^2 氧化成Fe^3 ,继续用抗坏血酸标准溶液滴定,从而计算出Fe^3 、Fe^2 含量。 相似文献
19.
《国际聚合物材料杂志》2012,61(18):963-970
Semi-interpenetrating polymer networks (IPNs) are prepared from poly methyl methacrylate and 2-hydroxy ethyl acrylate networks with the presence of super-paramagnetic ferric oxide nanoparticles (<30 nm) through free radical polymerization. PMMA/PHEA semi-IPNs having blend ratio 60:40, 50:50, 40:60 (wt/wt) are characterized with respect to swelling, crosslink density, FTIR, DSC, TGA, SEM, and drug loading and drug release properties. Bactericidal effect on IPNs is checked by cell growth study of E. coli. 相似文献
20.
Negin Pournoori Prof. Mohammad Ali Oghabian Rasoul Irajirad Dr. Samad Muhammadnejad Dr. Hamid Delavari H. 《ChemMedChem》2022,17(11):e202100708
Immune checkpoint blockade, considered a revolutionary approach in cancer treatment, is only effective in patients with high tumor-infiltrating lymphocytes (TILs). This work aimed to investigate the feasibility of targeted contrast agent (CA) based on dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs-DEX) for TILs detection by magnetic resonance imaging (MRI) studies. To do so, we synthesized an MRI CA by conjugating SPIONs-DEX to an anti-CD3 monoclonal antibody via cyanogen bromide as a cross-linker. In vitro assessments demonstrated the higher labeling efficiency of the developed CA to CD3+ lymphocytes compared to SPIONs-DEX. In vivo MRI of a xenograft model of CD3+ lymphocytes revealed the significant signal loss after the intravenous injection of the bioconjugate by ∼34 % and 21 % in T2*-weighted and T2-weighted images, respectively. The histopathological evaluation of xenograft tumors confirmed the labeling of lymphocytes by the targeted CA. This approach could open up a new horizon in the non-invasive assessment of TILs to identify patients eligible for immunotherapy. 相似文献