食用油中反式脂肪酸的气—质分析法研究

建立气相色谱—质谱法测定食用植物油中反式脂肪酸的分析方法。食用植物油中的脂肪酸甲酯化后,经强极性毛细管柱SP—2560分离,采用质谱全扫描Scan方式得到各组分质谱图与标准谱库做相似度检索来定性,面积归一化法定量。用所建立的方法测定了6种常见食用植物油中反式脂肪酸含量,该法测定食用植物油中的反式脂肪酸含量前处理简单、快...     

两种色谱柱分离37种脂肪酸组分研究

分别采用CD-2560色谱柱和DB-WAX色谱柱对37种脂肪酸标样和油样脂肪酸组分进行分离,探索了色谱柱流速、分流比和程序升温对分离效果的影响。利用已知CD-2560色谱柱对37种脂肪酸混标组分出峰顺序、标样质量浓度和油样比对,理顺了DB-WAX色谱柱测定37种脂肪酸混标中各组分出峰顺序和名称。结果表明,这两种色谱柱的最佳分离条件为:混标质量浓度1 mg/mL,柱流速0. 55 mL/min,分流比70∶1,程序升温为100℃保持5 min,以3℃/min上升至230℃(CD-2560色谱柱保持30 min,DB-WAX色谱柱保持50 min)。采用这两种色谱柱用于5种油样中脂肪酸组分含量的测定,分离效果几乎一致,说明CD-2560色谱柱和DB-WAX色谱柱均能较好地应用于植物油中37种脂肪酸组分的测定。     

食用植物油的高分辨气相色谱分析

为评估食用植物油的营养价值,该文分析了常用食用植物油中的脂肪酸组成和各种油的营养特色。用KOH—CH3OH溶液将植物油皂化后,在三氟化硼作为催化剂的作用下,用CH3OH将样品甲酯化,正己烷提取甲酯化产物。以毛细管柱DB-23作为分离柱,用气相色谱法测定植物油的脂肪酸组成。大豆油、葵花籽油和玉米油中含有50％-60％的亚油酸和20％-30％的油酸,营养均衡合理;花生油和芝麻油中油酸与亚油酸含量相当,有35％-45％,易于人体吸收;菜籽油中含有45％的油酸和15％芥酸,对人体健康有不利作用;橄榄油和茶油中含有75％-80％的油酸,红花籽油中含有约80％的亚油酸,有降低胆固醇功效。     

定量结构-色谱保留相关联方法分析食用植物油中脂肪酸组成

采用气相色谱-氢火焰离子化检测器分别测定8 种食用植物油中脂肪酸组分的色谱保留时间,并结合定量结构-色谱保留相关联（quantitative structure-retention relationship,QSRR）方法和标准品分别对每种植物油中的脂肪酸组分进行定性分析,将这2 种方法的定性分析结果作对比,以验证QSRR方法在食用油中脂肪酸定性分析方面的实际应用能力。结果表明：QSRR方法的定性结果与标准品对脂肪酸的定性结果基本一致,QSRR方法具有很好的实际应用性,为脂肪酸的测定提供了一条新途径,对食用植物油中脂肪酸的分析测定有一定的参考价值。     

我国主要食用植物油中反式脂肪酸的研究

以GB/T 22110-2008为检测方法,对我国市场上销售的主要食用植物油(调和油、大豆油、花生油、芝麻油)中反式脂肪酸含量进行测定.结果显示:十八碳类反式脂肪酸有6种检出,同一油种具有相同种类的反式脂肪酸,各种类反式脂肪酸含量变化具有同步性,并呈显著性相关,各类反式脂肪酸在产生时具有相同的异构几率;当一种食用植物油的某类反式脂肪酸总含量接近或超过相应脂肪酸组成含量时,这份油样肯定不是单一品种的油样;反式脂肪酸主要来源于食用植物油的精炼过程,尤其是脱臭过程;我国存在因为反式脂肪酸摄入量过高而影响健康的情况,消费者、企业及政府部门应对食用植物油中反式脂肪酸的含量引起足够的重视.     

气相色谱法测定常见植物油中脂肪酸

随着营养学研究发展,人们对脂肪酸认识不断增加。中国营养学会也推荐膳食中必需脂肪酸摄入理想比值,市场上也出现大量调和油,为了评估食用植物油营养价值,该研究分析常用食用植物油中脂肪酸组成和各种油营养特色。方法:用氢氧化钾-甲醇溶液将植物油皂化后,在三氟化硼作为催化剂作用下,用甲醇将样品甲酯化,正己烷提取甲酯化产物。以毛细管柱DB-23作为分离柱,用气相色谱法测定植物油脂肪酸组成。结果:大豆油,葵花籽油,玉米油中含有50％-60％亚油酸和20％-30％油酸,营养均衡合理;花生油,芝麻油中油酸、亚油酸含量约35％-45％,易于人体吸收;菜籽油中含有45％油酸和15％芥酸,芥酸对人体健康有不利作用;橄榄油,茶籽油中含有75％-80％油酸,红花籽油中含有约80％亚油酸,有降低胆固醇功效。     

气相色谱法测定食用植物油中乙基麦芽酚的含量

建立了气相色谱法测定食用植物油中乙基麦芽酚含量的方法。食用植物油用80%甲醇提取,色谱柱分离。乙基麦芽酚含量在0.5-30μg/mL的范围内线性关系良好,线性方程分别为Y=2.3716X+0.0487,r均为0.9997。乙基麦芽酚回收率86.56%,RSD为3.60%。该方法可以作为食用植物油中乙基麦芽酚非法添加物的检测方法。     

15种食用植物油脂肪酸的气相色谱-质谱分析

建立了气相色谱-质谱联用法测定食用植物油中脂肪酸组成的方法。方法选用NaOH-甲醇溶液作为衍生试剂,色谱柱为极性毛细管柱,分流比为20:1,对15种常见植物油中的脂肪酸组成进行了分析,并用SPSS软件对植物油样品进行了系统聚类分析。结果表明,植物油中的脂肪酸组成以棕榈酸（C16:0）、硬脂酸（C18:0）、油酸（C18:1）和亚油酸（C18:2）为主;除椰子油外,其余14种植物油中不饱和脂肪酸含量均达75 %以上,大于饱和脂肪酸含量。     

用气相色谱——质谱联用法测定植物油中脂肪酸的组成

用正已烷溶解植物油,将样品用氢氧化钾-甲醇溶液直接皂化和甲酯化后,以毛细管柱DB-WAX作为分离柱,用气相色谱-质谱法(GC/MS) 快速测定植物油的脂肪酸组成。     

掺混棕榈油测定方法研究

植物油的掺混可用气相色谱法快速鉴定,根据不同植物油脂肪酸组分和含量不同来进行鉴别.采用三氟化硼法对测定样品进行甲酯化,填充柱气相色谱法分离检测,方法快速、准确.     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press