Changes in the RNA content of the blood plasma in rats with Ehrlich ascitic cancer

The RNA content in the blood plasma of tumour-bearing animals correlates with the tumour growth stage. The development of Ehrlich carcinoma in rats is followed by the RNA increase in the blood plasma, while spontaneous regression of the tumour is accompanied by a decrease of the RNA content in the blood plasma of rats with Ehrlich carcinoma almost to the normal level. The fractional spectrum of the blood plasma RNA in the tumour bearing rats is similar to that of the cell-free ascitic fluid of rats.     

Modifying effects of changes connected with pregnancy on 1,2-dimethylhydrazine-induced carcinogenesis in rats

The modifying effect of pregnancy and lactation on carcinogenesis induced by single injection of 1,2-dimethylhydrazine to female rats 30 days before mating has been studied. The total incidence of malignant tumours in rats with pseudopregnancy, single pregnancy, pregnancy and lactation, repeated pregnancies was lower in comparison with virgin animals (75, 44, 68, 59 and 93%, respectively). Colon adenocarcinoma incidence in rats with single pregnancy or repeated pregnancies was lower than that in virgin rats (42, 49 and 76%, respectively). Protective effect was observed mainly in descending colon. The mesenchymal kidney tumours were not developed at all in rats with single pregnancy. In virgin animals it was 31%. The inhibition of tumour incidence in the liver (cholangioma, cholangiocellular carcinoma) was observed in rats with single pregnancy or pregnancy and lactation in comparison with virgin control (3, 5 and 24%, respectively).     

粉防己碱对血管性痴呆大鼠学习记忆能力及神经元凋亡的影响

目的：探讨粉防己碱（Tet）对血管性痴呆（VaD）大鼠学习记忆能力和海马神经元凋亡的影响。方法：将40只大鼠随机分为假手术组、模型组、Tet干预组（含10和30mg/kg两个剂量组）,采用双侧颈总动脉永久性结扎（2-VO法）建立VaD大鼠模型。造模12周后进行Morris水迷宫实验检测大鼠行为学,HE染色观察海马CAl...     

Cationic protein content in the leukocytes of rats with Pliss' lymphosarcoma and 7,12-dimethylbenz(a)anthracene-induced sarcoma

The level of intraleukocytic cationic proteins is studied in rats with the transplanted Pliss lymphosarcoma and sarcoma induced by 7,12-dimethylbenz(a)anthracene (DMBA). The amount of cationic proteins was found to decrease in granulocytes both of mongrel and Wistar rats with transplantable tumours. The phenomenon is typical of the rats with the DMBA-induced tumour.     

Interactions of "ultra-low" doses of naltrexone and morphine in mature and young male and female rats

Sex and age influence morphine analgesia in humans and animals. Mature rats show greater morphine analgesia in males than in females. Ultra-low doses of naltrexone enhance morphine analgesia. In mature rats (18-22 weeks), naltrexone (0.002-2.0 mg/kg)-morphine (2 mg/kg) cotreatment enhanced morphine analgesia in females, an effect inversely related to naltrexone dose. Conversely, in mature male rats, naltrexone tended to decrease morphine analgesia with increasing dose. In young rats (8-10 weeks), morphine analgesia was unrelated to sex and in both sexes the naltrexone-morphine interaction was negligible. These data show that dose, age, and sex alter the naltrexone-morphine interaction in rats.     

靶向bcl-2小分子化合物抑制角膜新生血管研究

目的探讨靶向bcl-2小分子化合物(Z24)对大鼠角膜新生血管的抑制作用。方法采用完全随机化方法将30只大鼠分为2组,每组15只鼠(15只眼),应用角膜基质缝线法诱导角膜新生血管,采用灌胃给药。治疗组:Z24混悬液(80mg/kg);对照组:等量不含Z24的空白液;自术后第1天起,1次/d,连续用药7d。每组预先标定8只大鼠进行新生血管的定量观察,其余大鼠进行组织学观察。结果缝线后第7天,治疗组新生血管的面积明显小于对照组(P<0.05)。结论全身应用Z24能够延缓角膜新生血管的生成。     

Neutral DNAse activity in rat tissues in radiation carcinogenesis

The activity of neutral DNAse and its natural protein inhibitor was determined in the liver, brain and spleen of albino rats with malignant tumours of mammary gland and other organs. The tumours appeared 14-20 months after the irradiation of rats with fast neutrons (0.5-1.0 Gy). It is shown that the activity of DNAse I and its protein inhibitor increases in the liver and brain of tumour-bearing rats, while the enzyme and inhibitor capacity to form the nuclease-inhibitor complex decreases, especially in the liver. It is suggested that the increase in the DNAse activity in the tumour-bearing rats liver is important in the antitumour activity of the organism.     

Cytogenetic characteristics of H3 oxide induced myeloid leukemia in Wistar rats

Frequency and spectrum of chromosomal aberrations were studied in hemopoietic cells of Wistar rats myeloid leukaemia induced by the prolonged exposure to tritium oxide. Differences in quantitative and structural damages of chromosomes were revealed by the cytogenetic assessment of eighteen rats with acute myeloid leukaemia (AML) and six rats with chronic myeloid leukaemia (CML). The most characteristic chromosomal aberrations for AML appeared to be rearrangements in chromosomes 1-3. In CML translocations by one of chromosomes 15-20 coupled with defects in the groups of chromosomes 5-10 seemed to be nonrandom.     

Blood‐brain barrier permeability in rats exposed to electromagnetic fields used in wireless communication

Biological effects of radio frequency electromagnetic fields (EMF) on the bloodbrain barrier (BBB) have been studied in Fischer 344 rats of both sexes. The rats were not anaesthetised during the exposure. All animals were sacrificed by perfusion–fixation of the brains under chloralhydrate anaesthesia after the exposure. The brains were perfused with saline for 3–4 minutes, and thereafter perfusion fixed with 4% formaldehyde for 5–6 minutes. Whole coronal sections of the brains were dehydrated and embedded in paraffin and sectioned at 5 µm. Albumin and fibrinogen were demonstrated immunohistochemically and classified as normal versus pathological leakage. In the present investigation we exposed male and female Fischer 344 rats in a Transverse Electromagnetic Transmission line chamber to microwaves of 915 MHz as continuous wave (CW) and pulsemodulated with different pulse power and at various time intervals. The CWpulse power varied from 0.001 W to 10 W and the exposure time from 2 min to 960 min. In each experiment we exposed 4–6 rats with 2–4 controls randomly placed in excited and nonexcited TEMcells respectively. We have in total investigated 630 exposed rats at various modulation frequencies and 372 controls. The frequency of pathological rats is significantly increased (p < 0.0001) from 62/372 (ratio: 0.17 ± 0.02) for control rats to 244/630 (ratio: 0.39 ± 0.03) in all exposed rats. Grouping the exposed animals according to the level of specific absorbed energy (J/kg) give significant difference in all levels above 1.5 J/kg. The exposure was 915 MHz microwaves either pulse modulated (PW) at 217 Hz with 0.57 ms pulse width, at 50 Hz with 6.6 ms pulse width or continuous wave (CW). The frequency of pathological rats (0.17) among controls in the various groups is not significantly different. The frequency of pathological rats was 170/481 (0.35 ± 0.03) among rats exposed to pulse modulated (PW) and 74/149 (0.50 ±0.07) among rats exposed to continuous wave exposure (CW). These results are both highly significantly different to their corresponding controls (p <0.0001) and the frequency of pathological rats after exposure to pulsed radiation (PW) is significantly less (p < 0.002) than after exposure to continuous radiation (CW).     

电针足三里对脓毒症大鼠促炎症因子所致心肌损伤的保护作用

目的研究电针足三里穴(ST36)对脓毒症大鼠促炎症因子所致心肌损伤的保护作用。方法雄性SD大鼠32只,采用盲肠结扎穿孔术(CLP)制备大鼠脓毒症模型,随机分为电针(Electro-Acupuncture,EA)足三里组(EA组)、CLP+假电针(ShameEA)组(SEA组)、迷走神经切断(Vagotomy,VA)+CLP组(VA组)和迷切后CLP再电针组(VA/EA组)4组,每组8只。EA组持续针刺双侧足三里穴1h(2mA,2-100Hz);SEA组采用相同频率和强度刺激非经非穴(足三里穴外侧旁开0.5cm)。VA组先切断腹腔迷走神经再施行CLP术。各组大鼠于CLP术后6h,取血检测血浆肌酸激酶同工酶(CK-MB)活性;然后处死动物,取心肌组织,测定肿瘤坏死因子-α(TNF-α)水平、一氧化氮(NO)含量、随过氧化物酶(MPO)活性以及组织含水率。结果CLP术后6h,EA组心肌组织促炎症因子TNF-α、NO、MPO水平、CK-MB活性以及组织含水率均显著低于其余3组(P<0.05);VA/EA组和VA组TNF-α、NO、MPO水平、CK-MB活性及组织含水率均高于SEA组(P<0.05);VA/EA组与VA...     

倍他福林抗早产体内实验研究

目的:研究倍他福林体内抗早产作用.方法;利用多种动物早产模型,观察倍他福林对晚期妊娠大鼠分娩启动的影响、倍他福林对清醒足月妊娠大鼠产程的影响以及倍他福林对前列腺素F2a致小鼠早产的影响.结果:倍他福林可以延长晚期妊娠大鼠妊娠时间和产程;增加清醒足月大鼠各子鼠娩出时间差,提高子鼠出生死亡率;降低前列腺素F2a致早产小鼠早...     

苯那普利对糖尿病大鼠肾小球基底膜负电荷位点的影响

目的：探讨血管紧张素转换酶抑制剂苯那普利（benezapril）对糖尿病大鼠肾小球基底膜负电荷位点的影响。方法：链脲佐菌素（STZ）腹腔注射诱发大鼠糖尿病模型，苯那普利灌胃，2周、12周取肾脏皮质组织，采用组织化电镜的方法，定量分析肾小球基底膜负电荷位点的变化。结果：糖尿病模型组大鼠肾小球基底膜的负电荷位点较对照组显著减少，苯那普利治疗组的肾小球基底膜负电荷位点较糖尿病模型组显著增多，与对照组相似。结论：苯那利对实验性糖尿病大鼠肾小球基底膜的负电荷位点有保护作用。     

低剂量伽玛刀照射实验性癫痫老龄大鼠海马神经元超微结构观察

目的:观察低剂量伽玛刀照射实验性老龄癫痫大鼠海马神经元超微结构的变化。方法:采用青霉素定位浸润建立老龄大鼠癫痫动物模型,将57只老龄大鼠分为对照组、癫痫组和癫痫后伽玛刀照射组。照射周边剂量12Gy,等剂量曲线为50%,0.5 h～60 d后取靶区海马制备电镜样品,透射电镜观察。结果:对照组海马神经元的超微结构正常;癫痫大鼠可见神经元细胞器明显空化,部分神经元凋亡;伽玛刀照射组早期与癫痫模型组基本一致,中期和晚期部分结构得以恢复,线粒体修复较为明显。结论:老龄癫痫大鼠经低剂量伽玛刀照射,中、晚期海马神经元的超微结构改变得以修复,可为临床治疗老龄癫痫提供形态学依据。     

Effects of ovariectomy on bone morphology in maxillae of mature rats.

Postmenopausal oestrogen deficiency results in bone loss (osteoporosis) in humans and experimental animals. The loss of trabecular bone in the ovariectomized (OVX) rat provides a useful experimental model of post-menopausal osteoporosis. At 5 months after ovariectomy of 3-month-old female rats, the mid and distal femurs and maxillae were dissected and processed for quantitative backscattered electron microscopic examinations. Histomorphometric analysis of femurs in OVX rats showed significant loss in metaphyseal trabecular bone areas compared with sham-operated controls; no significant bone loss was observed in the cortical bone areas of mid-diaphyses in OVX rats. Net bone areas in the maxillae of OVX rats was similar to that of sham-operated controls. Bone structure of maxillae in OVX rats was also similar to that in controls. Our results suggest that, in this animal model of osteoporosis, prominent bone loss occurs mainly in the bone areas formed by endochondral ossification such as distal femurs, but those areas formed by intramembranous ossification such as mid-femurs and maxillae sustained less effects by OVX.     

Enhancement of Spatial Learning-Memory in Developing Rats via Mozart Music

This paper studies the effect of musical stimulations on the capability of the spatial learningmemory in developing rats by behavioral and electrophysiological techniques. Rats, which are exposed to Mozart's Sonata for Two Pianos in D Major, complete learning tasks of the Moriss water maze with significantly shorter latencies, and the power spectrum of alpha band of electrohippocampogram (EHG) significantly increase, compared with the control rats and rats exposed to the horror music. The results indicate that if given the stimulation of Mozart music in the developmental period of the auditory cortex, the capability of the spatial learning-memory can be significantly changed. The enhancement of alpha band of EHG may be related to the change of this function mainly.     

芸香苷对急性胰腺炎大鼠胰腺毛细血管通透性的影响

目的研究芸香苷(Rutoside,Ru)对大鼠急性胰腺炎(Acute Pancreatitis,AP)胰腺毛细血管通透性的影响。方法wistar大鼠48只,随机分为6组,每组8只,分别建立大鼠假手术(SO)、急性胰腺炎(AP)、芸香苷(Ru)高、中、低剂量及丹参(DS)组,在制模后3、6h经下腔静脉取血,测定血清淀粉酶含量和磷脂酶A2活性。切取胰腺标本组织,对胰腺组织进行病理检查和胰腺微血管通透性测定。结果术后6h,各组大鼠胰腺毛细血管通透性分别为:SO组(89.10±26.02)、AP组(405.25±234.48)、Ru高剂量组(132.79±56.72)、Ru中剂量组(203.83±89.06)、Ru低剂量组(263.84±113.02)、DS组(191.22±77.05)。各组间比较,6hRu高剂量和中剂量组胰腺毛细血管通透性低于AP组(P<0.01)。结论Ru能明显降低AP大鼠胰腺毛细血管通透性,减轻胰腺组织病理性损害。     

支架连接代替血管吻合建立大鼠肾移植模型

目的探讨抗淋巴细胞血清(ALS)联合他克莫司(FK506)在诱导大鼠肾脏移植免疫耐受中的作用。方法以SD大鼠作为供体,Wistar大鼠作为受体,建立大鼠肾移植模型。对照组(A组)仅行肾移植,术前术后未予免疫干预;ALS组(B组)术后当天腹腔注射ALS,连续应用至术后第10天;FK506组(C组)术后当天开始应用FK506,连续应用至术后第10天;联合组(D组)联合应用ALS与FK506。术后观测各组受者存活时间、移植肾功能、移植肾血供、免疫耐受状态等。结果D组平均存活时间为(37.0±5.3)d,与A组(7.4±1.6)d、B组(16.3±4.7)d及C组(17.5±5.3)d比较,差异均有统计学意义(P<0.05);手术后1周检查A组移植肾血供差,平均肾血管阻力指数0.80±0.06;B组和C组移植肾血供良好,平均阻力指数分别为0.62±0.07、0.63±0.08;D组移植肾血供良好,术后1周与术后20d平均阻力指数分别为0.61±0.04、0.62±0.03;D组与A组相比较差异有统计学意义(P<0.05)。结论ALS联合FK506能够延长受者存活时间和促进免疫耐受的诱导。     

Enhancement of Spatial Learning-Memory in Developing Rats via Mozart Music

This paper studies the effect of musical stimulations on the capability of the spatial learningmemory in developing rats by behavioral and electrophysiological techniques. Rats, which are exposed to Mozart＇s Sonata for Two Pianos in D Major, complete learning tasks of the Moriss water maze with significantly shorter latencies, and the power spectrum of alpha band of electrohippocampogram （EHG） significantly increase, compared with the control rats and rats exposed to the horror music. The results indicate that if given the stimulation of Mozart music in the developmental period of the auditory cortex, the capability of the spatial learning-memory can be significantly changed. The enhancement of alpha band of EHG may be related to the change of this function mainly.     

铬制肠线与丝线制作CCI模型效果比较

目的比较利用铬制肠线和丝线制作CCI疼痛模型痛阈变化的区别。方法将30只健康雄性SD大鼠随机分为铬制肠线组、丝线组和假手术组,每组10只。铬制肠线组和丝线组分别用铬制肠线和丝线对大鼠右侧坐骨神经进行松结扎,假手术组只暴露右侧坐骨神经但不进行任何处理。建模后测量各组大鼠热缩足反射阈值(TWT)和机械性缩足反射阈值(MWT)。结果铬制肠线组和丝线组均能引起大鼠痛阈的降低(与假手术组比较P<0.05),铬制肠线组和丝线组间MWT无明显区别(P>0.05),铬制肠线组TWT低于丝线组(P<0.05)。结论铬制肠线制作CCI模型较之丝线制作CCI模型能引起更为明显的热痛阈降低。     

大白鼠体内照射治疗白细胞减少症

为了研究砷化镓激光光纤引入机体后对骨髓产生的影响,我们将砷化镓激光光纤直接引入大白鼠体内照射,连续观察了对大白鼠白细胞减少症的治疗作用。现将研究概况介绍如下。 一、材料与方法 1.仪器:使用重庆大学激光光纤科研小组自行装配的XLL型半导体砷化镓激光光纤治疗仪。砷化镓激光以0.5～1.5m长的光纤耦合后插入动物体深部组织。光纤输出端的光功率为1.0～1.3mW,