共查询到20条相似文献,搜索用时 78 毫秒
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串联质谱在商品染料及助剂分析中的应用 总被引:1,自引:0,他引:1
串联质谱是一种发展迅速且能提供丰富信息的质谱新技术,成功地应用于未知物的结构鉴定,尤其是用于混合物的直接分析。本文介绍了串联质谱的原理,仪器和基本操作模型,并举例说明其在混合物分析中的应用。 相似文献
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建立了反相高效液相色谱法测定百合中氨基酸含量的方法。采用HypersilODS2C18色谱柱(4.6mm×250mm,5μm),流动相A乙酸钠+四氢呋喃+三乙胺;流动相B:乙酸钠+甲醇+乙腈,柱温35℃,检测波长330nm。梯度洗脱程序为(t(梯度洗脱时间),B(流动相B)%)为(22,40);(23,60);(23.6,60);(26,65);(26.1,70);(26.6,80);(31.9,90);(32,95);(39,100);(40,0)。测定结果表明,在此实验条件下能使百合中13种氨基酸在40min内得到较好的分离,特别是脯氨酸能得到很好的分离。除trp、gly外,其他氨基酸具有较好的线性(相关系数为0.8985~0.9997)和重复性(相对标准偏差≤5.0%)。该方法简便实用,实际样品分析结果令人满意。 相似文献
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Zijian Li Wanchun Sun Donglin Wu Xiang Gao Ningning Sun Ning Liu 《International journal of molecular sciences》2014,15(2):2465-2474
Amino acid substitutions in influenza A virus are the main reasons for both antigenic shift and virulence change, which result from non-synonymous mutations in the viral genome. Nucleocapsid protein (NP), one of the major structural proteins of influenza virus, is responsible for regulation of viral RNA synthesis and replication. In this report we used LC-MS/MS to analyze tryptic digestion of nucleocapsid protein of influenza virus (A/Puerto Rico/8/1934 H1N1), which was isolated and purified by SDS poly-acrylamide gel electrophoresis. Thus, LC-MS/MS analyses, coupled with manual de novo sequencing, allowed the determination of three substituted amino acid residues R452K, T423A and N430T in two tryptic peptides. The obtained results provided experimental evidence that amino acid substitutions resulted from non-synonymous gene mutations could be directly characterized by mass spectrometry in proteins of RNA viruses such as influenza A virus. 相似文献
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Assessment of Isoprostanes in Human Plasma: Technical Considerations and the Use of Mass Spectrometry 下载免费PDF全文
Yiu Yiu Lee Jean-Marie Galano Camille Oger Claire Vigor Reversat Guillaume Jérôme Roy Jean-Yves Le Guennec Thierry Durand Jetty Chung-Yung Lee 《Lipids》2016,51(11):1217-1229
Oxygenated lipid mediators released from non‐enzymatic peroxidation of polyunsaturated fatty acids (PUFA) are known to have functional roles in humans. Notably, among these lipid mediators, isoprostanes molecules are robust biomarkers of oxidative stress but those from n‐3 PUFA are also bioactive molecules. In order to identify and assess the isoprostanes, the use of mass spectrometry (MS) for analysis is preferable and has been used for over two decades. Gas chromatography (GC) is commonly coupled to the MS to separate the derivatized isoprostanes of interest in biological samples. In order to increase the accuracy of the analytical performance, GC–MS/MS was also applied. Lately, MS or MS/MS has been coupled with high‐performance liquid chromatography to assess multiple isoprostane molecules in a single biological sample without derivatization process. However, there are limitations for the use of LC–MS/MS in the measurement of plasma isoprostanes, which will be discussed in this review. 相似文献
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