共查询到20条相似文献,搜索用时 62 毫秒
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通过正交实验研究了絮凝剂产生菌F00产絮凝剂的条件及其发酵生理.结果表明,F00产絮凝剂的最佳条件为:初始pH值为6.5,培养温度为30℃,通气量为80 r·min-1,C/N为10;培养72 h絮凝剂活性最高达到72%.F00发酵过程中,絮凝剂生物合成最多是处于菌体快速生长之后、菌丝停止生长到开始进入繁殖阶段大量产生分生孢子的时期,因此可在此时收获絮凝剂. 相似文献
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一株聚乙烯醇降解酶产生菌的产酶条件优化 总被引:4,自引:0,他引:4
枯草芽胞杆菌WSH-062在摇瓶培养中能产生胞外的PVA降解酶。通过单因素及正交实验对其进行了发酵条件的优化。研究结果表明:该菌产生的PVA降解酶为诱导型酶;2 g/L的复合氮源(NaNO3和酵母粉的配比为1∶1)就能满足细胞生长和产酶的营养需要。正交实验分析得到最优的发酵培养基为:PVA 30 g/L,酵母粉12.2 g/L,NaNO3 10.1 g/L,MgSO4.7H2O 0.35 g/L,KH2PO4 3 g/L,pH值7.2。优化之后的PVA降解酶酶活达到5.00 U/mL,比优化前提高了4.75倍,并且重复性较好。 相似文献
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《应用化工》2022,(12)
对一株适用于采油的枯草芽孢杆菌发酵培养基组成和发酵条件进行了优化。通过实验室内的摇床发酵,得到优化后培养基为:蔗糖50 g/L,NaNO_3 3.4 g/L,NH_4Cl 1.1 g, KH_2PO_4 2.5 g/L,12H_2O·Na_2HPO_4 30 g/L,7H_2O·MgSO_4 0.8 g/L,脂肽平均产量为4.2 g/L,表面张力可降低至25.15 mN/m;同时,因为在采油工业中,每吨NH_4Cl比每吨NaNO_3的成本低30%左右,所以通过NH_4Cl的加入,减少了NaNO_3的用量,大大降低了发酵成本;最佳培养条件为:温度37℃,装液量150 mL/250 mL,发酵时间100 h。 相似文献
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研究了一株青霉菌P6 (Penicillium sp. P6)液体发酵产生木素过氧化物酶的工艺条件,考察了青霉菌P6在天然培养基稻米糠、玉米糠、麦糠和合成培养基GMY中的产酶情况,结果表明,麦糠是较理想的底物. 通过单因素方法研究了添加苯甲醇和MnSO4对酶活的影响;采用正交实验对MnSO4添加量、麦糠含量和培养基pH进行了研究,在实验范围内最佳酶活达到了2.69 U/mL. 进一步通过单双因素回归实验研究了麦糠和MnSO4的最佳含量,得到了最佳培养条件为孢子接种量1.1×106 mL-1,培养时间8 d,500 mL三角瓶装液量150 mL. 最佳培养基组成为麦糠50 g/L,初始pH 4.8, MnSO4 5.66 mmol/L. 相似文献
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一株产聚乙烯醇降解酶的紫色杆菌的发酵条件研究 总被引:2,自引:0,他引:2
从聚乙烯醇(PVA)污染环境中筛选出一株产聚乙烯醇降解酶活力较高的菌株WSH04-01,该菌株能够利用聚乙烯醇作为唯一碳源进行生长.通过一系列生理生化试验和16S rDNA序列分析结果,鉴定该菌株属于紫色杆菌属(Janthinobacterium sp.),实验室编号WSH04-01.这是目前国内外有关紫色杆菌产生PVA降解酶的首例报道.首先对菌株合成PVA降解酶的营养条件进行了考察,通过单因素试验和正交试验确定了菌株最优培养条件为PVA10 g·L-1,葡萄糖3 g·L-1,酵母膏6 g·L-1,K2HPO4 2 g·L-1,KH2PO4 0 25 g·L-1,MgSO4 0.05 g·L-1,CaCl2 0.05 g·L-1,FeSO4·7H2O0.02 g·L-1,NaCl 0.02 g·L-1.最适发酵温度为30℃,培养基初始pH为7.2,装液量为30 mL培养基(250mL摇瓶)-1,接种量为8%.在最优条件下,PVA降解酶酶活可以达到4.94 U·mL-1,略高于正交试验中的最高酶活(4.83 U·mL-1).同时利用凝胶渗透色谱得到分子量分布图,对最优发酵条件下发酵过程中聚乙烯醇的降解进行了验证. 相似文献
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罗汉果内生真菌SG-69的发酵培养液和菌体通过硅胶柱色谱、SephadexLH-20凝胶柱色谱和重结晶等方法分离,得到6个化合物,运用IR、。HNMR.13CNMR等波谱技术,结合化合物的理化性质、波谱数据与文献数据对照鉴定了其结构分别为麦角甾-5,7,22-三烯-3β一醇(1)、对羟基苯甲酸(2)、白僵菌酮(3)、苯乙酸(4)、尿囊素(5)和甘露醇(6),其中尿囊素(5)首次从罗汉果内生真菌中发现。研究结果表明,罗汉果内生真菌的次级代谢产物与宿主罗汉果化学成分有很大差异,有待进一步研究。 相似文献
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Silvia A. Villarreal-Soto Jalloul Bouajila Sandra Beaufort Denis Bonneaud Jean-Pierre Souchard Patricia Taillandier 《乙烯基与添加剂工艺杂志》2021,27(1):183-190
The production of bacterial cellulose has been limited due to its high cost and low productivity. Alternative low-cost sources of this biopolymer of high purity and biocompatibility are needed in order to benefit from its enormous potential. Kombucha tea is a trend functional beverage whose production is growing exponentially worldwide, and the bacteria present in this fermented beverage belonging to the genus Komagataeibacter are capable of producing a crystalline biofilm with interesting properties. Obtaining bacterial cellulose from Kombucha tea has already been studied, however several fermentation conditions are being optimized in order to scale-up its production. In this study, we characterized the bacterial cellulose produced from three different Kombucha fermentation conditions. The scanning electron microscopy images revealed the crystalline structure of the biofilms. The energy-dispersive x-ray analysis exhibited the chemical composition of the crystals. The thermogravimetric analysis showed a rate of degradation between 490 and 560°C and the differential scanning calorimetry confirmed the presence of crystalline and amorphous regions in the bacterial cellulose samples. The results suggested that crystalline cellulose could be obtained by varying the fermentation conditions of Kombucha tea. 相似文献
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大麦天然酵素抗氧化性能的初步探究 总被引:1,自引:0,他引:1
大麦天然酵素为大麦经过天然发酵过程得到的发酵液,富含抗氧化活性物质。对大麦天然酵素抗氧化性能进行了初步探究。结果表明,天然发酵过程制得的大麦酵素具有较强的抗氧化活性,对羟基自由基、DPPH自由基、超氧自由基和ABTS自由基具有较高的清除能力;大麦天然酵素展现了较高的抗氧化能力,其抗氧化活性的大小与酚类物质含量具有一定相关性。本研究结果可为天然酵素产品的研发和生产工艺的改进提供一定的支持,为大麦的多功能开发利用提供新的思路。 相似文献
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Experiments were performed to assess the effect of four foliar essential oil phenotypes from a coastal redwood (Sequoia sempervirens) population on isolates ofPleuroplaconema sp., its ubiquitous endophytic fungus. Isolates were exposed to essential oils extracted from their trees of origin and from other trees. The hypotheses tested were: (1) redwood leaf essential oils extracted from distinct trees would have a differential effect onPleuroplaconema sp. growth, and (2) growth of isolates from a particular tree would be differentially affected when exposed to essential oil phenotypes different from that of their tree of origin. The essential oil phenotypes were differentially inhibitory, but the pattern of inhibition did not support the second hypothesis.Pleuroplaconema sp. showed low average tolerance to all of the essential oils; two phenotypes reduced growth 70–80% and the other two 50–60% at the dose tested. The overall growth response of individual isolates to all treatments suggests that more than one fungus genotype per tree was represented in the experiment. The variability in tolerance of individual isolates to the essential oils was low for three phenotypes. The low tolerance ofPleuroplaconema sp. to redwood essential oils, in spite of its predominance and specialization in this conifer, is discussed considering: (1) the possible pathogenic ancestry of this fungus, and (2) that essential oil phenotypes may be important in controlling the activity ofPleuroplaconema sp. after it colonizes the leaf. 相似文献
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高效降氰真菌的分离筛选及降氰条件研究 总被引:4,自引:0,他引:4
从电镀废水、含氰污泥及电镀车间渡槽液中分离筛选出三株高效降氰真菌,并对其中Fw菌株氰化物降解条件进行了研究。研究结果表明,该菌株的最佳降氰条件为温度20-30℃、pH5-7、摇床转数100~180r/min、接种量6%-20%。在最佳降氰条件下,Fw菌株降氰率达到95%以上,剩余氰化物质量浓度低于1mg/L。该菌株可以直接利用CN-作为碳源和氮源,并且在培养基中不外加碳源和氮源时,氰化物剩余质量浓度分别为0.25mg/L和0.49mg/L,达到国家一级排放标准。本研究结果可以作为利用真菌处理含氰废水的依据。 相似文献
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在摇瓶中进行了兽疫链球菌诱变株NW-162发酵生产透明质酸的工艺研究,通过正交实验优化了最佳培养基组成,并考察了发酵过程中发酵温度、培养时间、pH值、装料系数等条件对该菌株发酵生产透明质酸的影响。实验表明:碳源及氮源对发酵过程影响最显著,优化所得最佳培养基组成为:葡萄糖40 g/L,复合氮源20 g/L,MgSO42g/L,钠盐3 g/L;在36℃、pH值7.0、装料系数为0.4的工艺条件下,发酵至27—30 h时达到最优发酵效果,收率可达0.464 g/L,比优化前提高2倍。 相似文献