Improving the quality of fresh‐cut pineapples with ascorbic acid/sucrose pretreatment and modified atmosphere packaging

The effects of pretreatment and modified atmosphere packaging (MAP) on the quality of fresh‐cut pineapples stored at 4°C were evaluated for 7 days. The pretreatment was conducted by immersing the pineapple slices in a solution containing 0.25% ascorbic acid and 10% sucrose for 2min. MAP contained 4% oxygen, 10% carbon dioxide and 86% nitrogen. Both the pretreatment and MAP could reduce the respiration rate, ethylene production, textural and colour deteriorations, as well as the overall sensory deterioration in fresh‐cut pineapples. MAP could restrain the growth of microbes, but the pretreatment showed little effect. Fresh‐cut pineapples exhibited wet surface and off‐flavour after storage at 4°C for 3 days, while the pretreatment and MAP maintained the quality for up to 7 days. Copyright © 2007 John Wiley & Sons, Ltd.     

Modified Atmosphere Packaging of Guava Fruit

Fully matured fresh guava fruits (Lucknow‐49) were precooled at 10 °C for 1 h and pretreated with 500‐ppm benomyl for 5 min. The fruits were packed in 25‐µm and 50‐µm LDPE bags with active modified atmosphere packaging of 3% O₂ + 5% CO₂, 6% O₂ + 5% CO₂ and 9% O₂ + 5% CO₂ gas concentrations and stored at 5 ± 1 °C and 10 ± 1 °C temperatures as well as non‐packaged as control. The shelf life of guava could be increased up to 42 days by packaging in 50‐µm LDPE bags with a gas concentration of 9% O₂ + 5% CO₂ and stored at 10 °C without much change in physical, biochemical and sensory characteristics. Physico‐chemical changes viz., PLW, pulp to peel ratio, firmness, TSS, total sugar, ascorbic acid and titratable acidity were observed minimum for fruits packed in 50‐µm LDPE bags at 3% O₂ + 5% CO₂ gas concentration at 5 °C storage temperature, but sensory score decreased drastically after 21 days of storage. Chilling injury was observed for the fruits packed at 5 °C storage temperature after 21 days of storage, and it was severe in 25‐µm LDPE bag at later stage. Control fruits over‐ripened with the highest weight loss (12.47%) on 6 days of storage and discarded from the storage. Copyright © 2014 John Wiley & Sons, Ltd.     

低温气调包装对鲜猪肉保鲜效果的影响研究

按5种气调方式包装新鲜猪肉,于低温(约3.79℃)下储存,检测了鲜猪肉挥发性盐基氮(TVB-N)含量、pH值、感官指标和包装内的气体组分含量等指标。实验结果表明:采用高氧气调包装,鲜肉货架寿命长达24d,且能够保持猪肉的鲜红色,较适宜用于鲜肉冷链运输和货架销售;抑菌剂在低温储存环境中,能将鲜猪肉的货架寿命延长25%。     

Microbiological,colour and sensory properties of fresh beef steaks in low carbon monoxide concentration

Steaks from gluteus medius m. (GM) and longissimus dorsi m. (LD) were packaged in atmospheres containing two low levels of CO (0.2 and 0.4%) in combination with 21% O₂ or without oxygen. The other components of the modified atmosphere packaging systems were carbon dioxide (30%) and nitrogen (balance). Steaks were evaluated on 0, 7, 14 and 21 days of storage at about 2^oC to investigate the effects of the gas mixtures on the microflora, instrumental (CIE L*a*b*) and visual colour and odour. The preservation of colour and odour of GM and LD steaks packaged under low carbon monoxide was best achieved using anoxic mixtures containing 0.2% CO and 30% CO₂. Steaks packaged without oxygen retained a desirable colour and practically did not present a visible discoloration or perceptive off‐odour along the entire storage time. Colour stabilization with 0.2% CO and 30% CO₂, without O₂, following storage up to 21 days at 2°C did not increase microbial risk of products. The increase in CO from 0.2 to 0.4% had a negative effect on the colour of LD steaks which was regarded as ‘artificial’ by some panellists. The higher stability achieved in this study using 0.2% CO may result from increased stability of the colour from Bos indicus × Bos Taurus cross. Discoloration and off‐odour were evident in the GM and LD steaks packaged aerobically after 14 days of storage when the counts of deteriorant bacteria achieved approximately 6.5 to 9.7 log CFU/g. Highest bacteriostatic effect could be obtained if the volume of injected CO₂ was sufficient to saturate the meat and keep in balance around the product during storage. Copyright © 2010 John Wiley & Sons, Ltd.     

Evaluation of the Effectiveness of a New Antimicrobial Active Packaging for Fresh Atlantic Salmon (Salmo Salar L.) Shelf Life

Nowadays, consumers demand for fresh, high‐quality seafood products continues to climb, especially in affluent, developed nations. At the same time, there is increasing attention regarding improved utilization of seafood for human consumption; Atlantic salmon is the main species in Chile aquaculture, and most of the salmon are exported as fresh fish to USA. It is well documented that low storage temperature inhibit bacterial growth and biochemical degradation, thus facilitating prolonged shelf life. Partly freezing the outer fillet layer from ?0.5°C to ?2.0°C (superchilling) has proven to be more efficient in inhibiting biochemical changes and bacterial spoilage compared with traditional chilling. Nevertheless, antimicrobial active packaging offers the possibility to increase product shelf life through the reduction of the microbiological damage of the product during its transportation, and thus increasing consumer safety and health. The main objective of this study was to compare the effects of an active antimicrobial packaging on microbiological growth, sensory attributes and instrumental quality measurements of raw Atlantic salmon (Salmo salar) fillets. Flexible plastic films containing Thymol as antimicrobial agent were used for salmon packaging. Salmon were stored at 2°C, 4°C and 8°C. Analyses of aerobic mesophilic bacteria and psicrophilic bacteria count were carried out at 1, 3, 8, 11, 15 and 18 days. L*a*b* colour evolution, overall sensorial properties and chemical evolution were studied. Application of active antimicrobial packaging for fillet salmon reduced the microbial count for least 18 days at 2°C. No modification of the sensorial properties was detected by the sensorial panel, indicating that the antimicrobial compound used in the present study, thymol, is compatible with salmon packaging. Copyright © 2011 John Wiley & Sons, Ltd.     

Production and Application of Active Packaging Film with Ethylene Adsorber to Increase the Shelf Life of Broccoli (Brassica oleracea L. var. Italica)

Broccoli (Brassica oleracea L. var. Italica) florets were packaged in low‐density polyethylene (LDPE) bags with or without ethylene adsorber under passive modified atmosphere and then stored at 4°C for 20 days. LDPE films with (8% Tazetut® masterbatch, M2) or without ethylene adsorber (M1) were tested. The effects of modified atmosphere packaging treatments on gas concentrations (O₂, CO₂ and ethylene) in the headspace, the mass loss, colour, texture, pH, total soluble solids, chlorophyll content, total phenolic content and sensory quality of packaged broccoli were determined by comparing with unpackaged (control) florets. Results revealed that deterioration occurred quickly in control broccoli, manifested mainly by mass loss, chlorophyll degradation and stem hardening. Also, it was found unacceptable by sensory panel after 5 days. Conversely, in those florets packaged under modified atmosphere packaging, especially for LDPE with ethylene adsorber (M2), all changes related with loss of quality were significantly reduced and delayed with time. Additionally, total soluble solids and total phenolic content remained almost unchanged during the whole period. Ethylene concentration was determined as 61.8 ppm in M1 and 0.33 ppm in M2, respectively, at the end of the storage. Thus, broccoli packaged with M2 films had prolonged storability up to 20 days with high quality attributes, this period being only 5 days in unpackaged control broccoli. Oxygen concentration decreased below 1% after 5 days of storage in M1, and the shelf life of broccoli in these bags was limited to 5 days because of risk for anaerobic fermentation. Copyright © 2013 John Wiley & Sons, Ltd.     

Changes in the quality of abalone (Haliotis asinina Linnaeus) packaged under atmospheric air,vacuum and modified atmosphere

Changes in the quality of abalone (Haliotis asinina Linnaeus) meat packaged under modified atmosphere (MA) of 40% CO2/30% O2/30% N2, vacuum and atmospheric air in polyvinylidene chloride (PVDC)/nylon/oriented polypropylene (OPP) pouches, and stored at 2 ± 1°C were investigated. Biochemical indices, such as pH, total volatile basic nitrogen (TVB‐N), and trimethylamine (TMA) and sensory evaluation, as well as total plate counts of packaged abalone meat were determined periodically. TMA was not affected by the packaging conditions, and remained low during storage periods. A decrease in pH of the MA packaged abalones during the storage reflected the apparent absorption of CO2. The content of TVB‐N slowly increased in the MA packaged abalone, whereas those stored in atmospheric air markedly increased during the storage. The sensory quality of MA packaged abalones was shown to be acceptable up to 15 days, while atmospheric and vacuum‐packaged abalones were not acceptable after 3 days of storage. Copyright © 2007 John Wiley & Sons, Ltd.     

Effectiveness of modified atmosphere packaging in preserving a prepared ready‐to‐eat food

The effectiveness of modified atmosphere packaging (MAP) was evaluated for a combination prepared food (Korean braised green peppers with dry anchovies). From a preliminary storage test of the ready‐to‐eat dish at 10°C, the aerobic bacterial count on the green peppers was selected as a primary quality index. The effect of MAP with different CO₂ concentrations on the product quality at 10°C was also studied. MAP with a CO₂ concentration of ≥30% extended the lag time and/or reduced the growth rate of bacteria. Finally, the effect of different storage temperatures (5, 10, 15 or 20°C) on the shelf life of the product was investigated. Mathematical modelling of bacterial growth curves under stretch‐wrap air packaging and MAP with 60% CO₂/40% N₂ showed that MAP increased the hypothetical minimum temperature in the square root model that describes the temperature dependence of the lag time and growth rate. MAP conditions of 60% CO₂/40% N₂ extended the shelf life at 10°C by 130% (to 18.4 days) relative to that achieved with stretch‐wrap air packaging (7.9 days) based on the time taken to reach the quality limit of an aerobic bacterial count of 10⁵ CFU/g. The relative extension of shelf life achieved with MAP was greater at lower temperatures. Copyright © 2008 John Wiley & Sons, Ltd.     

Effects of PP‐based Nanopackaging on the Overall Quality and Shelf Life of Ready‐to‐eat Salami

A total of 1% nanoclay containing polypropylene (PP)‐nanocomposite and 1% nanoclay plus 5% poly‐beta‐pinene (PβP) containing PP‐active‐nanocomposite materials were produced and tested for packaging of sliced salami. The sliced salami was packaged using both nanofilms and multilayer film of PP/PA/EVOH/PE under vacuum, modified atmosphere packaging under 50% CO₂ and 50% N₂ and air and stored at 4 °C for 90 days. During storage, headspace gas composition; microbial, physical and chemical analyses; and sensory evaluation were performed. The antimicrobial effect of PβP containing nanomaterial was pronounced under vacuum, and no bacterial growth was observed for 75 days. An a* value decreased notably in all applications during storage and preserved best by the multilayered material under vacuum and high CO₂. Thiobarbituric acid reactive substances (TBARS) were 0.63 mg MDA/kg after the processing and gradually increased at all applications during increased storage. TBARS values of all vacuum applications were lower than that of modified atmosphere packaging applications. Rancid taste development was determined by sensory panelists when TBARS values were higher than 0.80 mg MDA/kg on the 90th day. There was no significant moisture loss, and no increase in hardness was determined during the whole storage time. The longest shelf life was 75 days for the sliced salami under vacuum and high CO₂ using the multilayer material. PβP containing nanomaterial provided 50 days of shelf life under vacuum, which is commercially considerable. Copyright © 2017 John Wiley & Sons, Ltd.     

Comparison of Various Packaging Films for Mango Export

This study compared the effects of the use of various types of packaging films in maintaining quality, preserving bioactive compounds and extending shelf life of the ‘Nam Dok Mai’ mango. The principal techniques for maintaining the quality of mangoes are based on modified atmosphere packaging using highly gas‐permeable films and an ethylene‐removing mechanism. The films studied were non‐perforated highly gas‐permeable film, non‐perforated ethylene‐absorbing highly gas‐permeable film (HNPE), micro‐perforated highly gas‐permeable films (HMP) and common non‐perforated polyethylene film (LNP). These films were compared with non‐packaging treatment, which served as a control (C). Gas composition (O₂, CO₂ and C₂H₄) in the package headspace was monitored during storage at 12°C. The quality of mangoes were assessed by weight loss, colour (L* a* b*), texture, total soluble solids, titratable acidity, total phenolics, total flavonoids, ethanol concentration and sensory quality. Equilibrium‐modified atmospheres of 5 kPaO₂ + 5 kPaCO₂ and 4 kPaO₂ + 5 kPaCO₂ were achieved in the non‐perforated highly gas‐permeable film and HNPE packs, respectively. Carbon dioxide accumulation was observed in the HMP and LNP packs where CO₂ reached 17 and 10 kPa, respectively, after storage for 10 days. The shelf life of mangoes was extended to 40 days with HNPE, 35 days with HNP, and 30 days with HMP, as compared with 20 days with control and 5 days with LNP. HNPE, the film incorporated with fine particles of mordenite framework inverted‐type zeolite with ethylene‐absorbing ability, could further extend the shelf life of mangoes in addition to modified atmosphere packaging alone. Copyright © 2011 John Wiley & Sons, Ltd.     

Effect of packaging materials and storage conditions on bacterial growth,off‐odour,pH and colour in chicken breast fillets

A comparison was made of the effect of different packaging materials on bacterial growth, off‐odour, pH and colour of chicken breast fillets stored at 4°C. For one of the packaging materials, the effects of temperature (4°C and 8°C) and initial oxygen present (0%, 2% and 4%) on bacterial growth, off‐odour, pH and colour in chicken breast fillets were also evaluated. Chicken breast fillets stored in the packaging material with the highest oxygen transmission rate (OTR) measured at actual storage conditions had the highest bacterial growth and the highest degree of off‐odour. Chicken breast fillets stored in packaging material mainly consisting of expanded PET had similar bacterial growth and off‐odour as in the barrier display film (BDF) packages, despite a smaller headspace volume and lower initial concentration of CO₂. No differences in discoloration and pH of the chicken breast fillets, due to storage temperature and amount of initial oxygen present, were found when one of the packaging materials was studied. In the early phase of the storage period, Pseudomonas spp. constituted the majority of the total viable counts, while after about 12 days, lactic acid bacteria dominated. At the end of the storage period, both Pseudomonas spp. and Enterobacteriaceae were present in high numbers. Significant differences in counts of Brochothrix thermosphacta were only obtained with initial presence of oxygen. The storage temperature had greater impact on microbial growth and off‐odour than the initial presence of oxygen in the packages. Copyright © 2004 John Wiley & Sons, Ltd.     

Quality Evaluation of Grated Graviera Cheese Stored at 4 and 12°C using Active and Modified Atmosphere Packaging

The effect of active [oxygen absorber (OA) combined with an ethanol emitter (EE)] and modified atmosphere (100% Nitrogen) packaging in combination with a high barrier experimental polyethylene terephthalate‐silicon oxides//low density polyethelene film on shelf‐life extension of grated Graviera cheese stored at 4 and 12°C was investigated. Microbiological (total viable counts, Pseudomonads, lactic acid bacteria, Enterobacteriaceae and Yeasts/Moulds), physico‐chemical (pH, thiobarbituric acid and colour) and sensory (odour and taste) changes occurring in the product were monitored as a function of treatment and storage time (10 weeks). Sensory shelf‐life was approximately 1, 1.5, 4.5, 6, 9 and at least 10 weeks for control samples (12 and 4°C), for N2 packaged samples (12 and 4°C) and samples packaged with the OA + EE (12 and 4°C), respectively. At the point of sensory rejection, yeasts and moulds increased from 2.00 to 3.60 and 5.55 log CFU/g for control samples stored at 4 and 12°C, respectively. Similarly, for samples stored under nitrogen, yeasts and moulds reached 2.00 and 2.32 log CFU/g at 4 and 12°C, respectively. Yeasts and moulds in samples with the OA + EE remained below 2 log CFU/g throughout the entire storage period, irrespective of storage temperature. pH varied between 5.72 and 6.49 depending on specific treatment. Malondialdehyde absorbance ranged between 0.05 for fresh samples and 0.79 mg/kg at the time of sensory rejection of samples. Light parameters L and b decreased while parameter a increased during storage, reflecting a gradual discolouration of all samples and especially those stored at 12°C.     

Effect of oxygen concentration on the shelf‐life of fresh pork packed in a modified atmosphere

Freshly slaughtered meat was packed in modified atmosphere packs (MAP) containing 5%, 15%, 25%, 35%, 45% and 55% O₂ along with 20% CO₂ in each package and stored for 8 days at 4°C. The samples were evaluated on the 8th day in terms of TVBN total volatile base nitrogen (TVBN) value, thiobarbituric acid reactive substances (TBARs) value, colour acceptability score, overall acceptability score and total microbial count. The result indicated better effects for 45% and 55% O₂‐containing MAP; however, the TBARs value of 45% O₂ atmosphere was significantly lower, without having significant differences in other evaluation criteria, than in the 55% O₂ package; hence 45% O₂ and 20% CO₂ gas mixture were selected as the most appropriate gas composition for fresh pork packaging. Copyright © 2005 John Wiley & Sons, Ltd.     

Effect of Pretreatment and Modified Atmosphere Packaging on Quality of dry Coleslaw mix

The aim of the study was to determine the effect of soaking in chlorinated water (100 ppm chlorine) or mild thermal treatment in water (55°C; 3 min) and modified atmosphere packaging on the quality and shelf life of dry coleslaw stored for 12 days at 4°C. Samples were packaged in low‐barrier film in modified atmosphere containing: 5/10/85, 20/25/55, 50/30/20, 70/30/0 %O₂/% CO₂/% N₂, and air atmosphere. In‐pack gas levels, sensory quality, microbial counts and cell permeability were determined. In comparison to non‐processed coleslaw, pretreatment of coleslaw, both consisting in soaking in chlorine solution and a mild thermal processing, resulted in a reduction by approx. 1 logarithmic cycle for counts of psychrophilic and lactic acid bacteria as well as mesophilic and psychrophilic yeasts, by 1 and 1.7 logarithmic cycle for coliform bacteria and by 2 up to 3 logarithmic cycles for Pseudomonas bacteria. Modified atmosphere (irrespective of its composition) used in product packaging was found to have no effect on the count of mesophilic bacteria. An elevated content of carbon dioxide in modified atmosphere (10, 25 or 30%) inhibited growth of psychrophilic bacteria and Pseudomonas bacteria up to 6 days of storage. In the case of coliform bacteria growth inhibition during storage was recorded both in the samples packaged in air atmosphere and in the atmosphere of 50/30/20 and 70/30/0 %O₂/% CO₂/% N₂. In samples packaged in the atmosphere of 70% O₂ and 30% CO₂ after 12 days the greatest cell permeability was recorded, which was connected with a deterioration of their microbiological quality. Copyright © 2015 John Wiley & Sons, Ltd.     

Shelf Life of Fresh Beef Stored in Master Packages with Carbon Monoxide and High Levels of Carbon Dioxide

Beef steaks from Gluteus medius, Longissimus dorsi, Psoas major and ground beef from Triceps brachii were packed in master packages containing expanded polystyrene trays overwrapped with polyvinyl chloride film under an anoxic atmosphere containing 0.2% CO, 60.0% CO₂ and 39.8% N₂ (T₁) or 0.2% CO/99.8% CO₂ plus oxygen scavengers. After 28 days of storage at 1 and 4°C, the meat quality was evaluated. The visual and instrumental colour of the beef steaks and ground beef were similar to that of fresh meat. Pathogenic bacteria were not detected, and psychrotrophic bacterial counts were lower than 7.5 log CFU/g. Copyright © 2012 John Wiley & Sons, Ltd.     

Effect of chitosan coating and vacuum packaging on the quality of refrigerated grilled pork

The effects of chitosan coating and vacuum packaging on the quality and shelf‐life of retail packaged grilled pork in a PVDC/nylon pouch during refrigerated storage at 2 ± 1°C were investigated. Grilled pork without chitosan coating and packaged in 100% air was used as the control sample. Firmness, pH, colour, peroxide values, microbial population and shelf‐life (as determined by sensory characteristics) of the samples were determined during the storage. The results showed that colour, peroxide values and microbial population coincided with sensory characteristics. The delayed growth of microbes in vacuum packaging can be attributed to inhibition by low levels of O₂. Chitosan coating was shown to minimize oxidation, as reflected by the peroxide values, colour changes and microbial counts of the samples. Vacuum‐packaged chitosan‐coated grilled pork showed negligible microbial growth and was found to be organoleptically acceptable throughout the storage period. Chitosan coating along with vacuum packaging provided a type of active packaging to maintain quality and extend the shelf‐life of grilled pork. Copyright © 2006 John Wiley & Sons, Ltd.     

Evaluation of Antimicrobial Active Packaging to Increase Shelf Life of Gluten‐Free Sliced Bread

This paper presents a complete study and comparison of the efficiency of three types of packaging system: active packaging, modified atmosphere packaging (MAP) and combinations of both. To choose the best option, we must take into account microbiological results as well as sensory quality; therefore, a complete sensory study and an in‐depth statistical study were carried out, in order to evaluate each option and their interactions. The results showed that active packaging considerably increases the shelf life of packaged food, so it could be an attractive option to extend the shelf life. The active packaging provided better sensorial properties than the MAP option. Positive factors like characteristic flavour and spongy texture had higher values in the absence of MAP, and negative properties like cinnamon flavour, crumbly texture and hardness reached higher values when MAP was present in the packaged food. So when microbiological and sensorial properties are taken into account, the active packaging is a better option than MAP to increase shelf life because it inhibits microbial growth while maintaining the high quality of sensorial properties for gluten‐free breads. Copyright © 2011 John Wiley & Sons, Ltd.     

Preservation of freshness of rainbow trout fillets packaged with carbon dioxide-nitrogen gas mixture (studies on gas-exchange packaging of fresh fish,part 2)

Gas-exchange packaging was applied to preserve freshness of rainbow trout fillets. The fillets were packaged in pouches of coextruded laminate (ethylene vinyl acetate/ polyvinylidene chloride-polyolefin) with a carbon dioxide-nitrogen gas mixture (N₂ : CO₂ = 6 : 4). Other fillets were wrapped with polyvinyl chloride film as a reference group. Changes in sensory score of colour and flavour, K-value and bacterial counts of the fillets were investigated during storage at 5°C. After 2 days the fillets wrapped with polyvinyl chloride film lost their gloss. In addition, an amine-like odour was detected after 3 days. The gas-packaged fillets, however, showed little change in colour, flavour and bacterial counts, and retained their fresh appearance for 3 days.     

Effect of different modified atmosphere packaging on microbial quality,oxidation and colour of a seasoned ground beef product (meatball)

The effects of different modified atmosphere packaging (MAP) on microbial quality, oxidation and colour of meatballs were investigated. Meatballs were prepared in our laboratory and packaged in vacuum or in different gases: combinations of O₂ (0, 2, 21%) and CO₂ (0, 33, 66, 100%) with N₂ as balance gas. Total microbial count, thiobarbituric acid reactive substances and colour were determined during storage at 3°C for 3 weeks. Total microbial count remained unchanged at around 6.3 LogCFU/g in vacuum and 100% CO₂ packages after 7 days, and slightly increased afterwards. The microbial count was higher in other packages and increased to 7.6 LogCFU/g after 21 days storage. Oxidation was inhibited by reduced O₂ and increased CO₂ in the packages. Packages with low O₂ maintained colour (a‐values) to a greater extent than the packages with higher O₂ levels. MAP containing 1–3%O₂ with 33%CO₂ inhibited microbial growth, oxidation and colour change in meatballs. Copyright © 2009 John Wiley & Sons, Ltd.     

Effects of modified atmosphere packaging conditions on quality preservation of powdered infant formula

The effect of different modified atmospheres (100% N₂, 100% CO₂, 70% N₂/30% CO₂, 50% N₂/50% CO₂, and air) on the quality preservation of powdered infant formula was investigated by a storage test at 30°C. Metal can packages of 400 g per unit were stored for 493 days with periodic measurements of package gas composition and product quality attributes, which included peroxide value (POV), pH, titratable acidity, bifidobacteria count, hydroxymethylfurfural content, surface colour, and solubility. In the air package, POV as an oxidative quality index increased highly along with oxygen concentration decrease, reaching a maximum of 58.7 meq/kg lipid at 350 days. Compared with the air package, POV increase of the product was significantly lower, with all the O₂‐excluded modified atmosphere packages showing a maximum of 28.5 to 29.3 meq/kg at 350 days. Packages of high CO₂ concentration experienced a pH decrease and an acidity increase of the stored product caused by dissolution of CO₂. The count of bifidobacteria in the product was maintained higher after 119 days in the package of 100% CO₂ than in the other packages. Hydroxymethylfurfural content showed a slight increase after an initial period of slight decrease, with little difference among the package treatments. There were no significant changes in product solubility and surface colour during storage. Considering that the high CO₂ atmosphere helps the survival of bifidobacteria and protects the product from oxidation without negative effects on the other quality attributes, it is suggested for use in packaging of powdered infant formula.