Experimental studies of surface plasmon polariton band gap effect

Surface plasmon polaritons (SPPs) propagation at a gold film surface covered by periodic arrays of ～40‐nm‐high scatterers arranged in a triangular lattice of different periods containing straight line defects is studied using collection scanning near‐field optical microscopy. The results reveal the dependence of the SPP band gap (SPPBG) effect manifested via the SPP reflection and guiding (along line defects) on the parameters of the surface structures (period, filling factor and lattice orientation). We find that the SPPBG effect is stronger along ΓK direction for all investigated periodic structures. Our results demonstrate that the SPPBG effect becomes less pronounced with a decrease of the filling factor and disappears for a filling factor close to 0.2. We also observe that the centre of the SPPBG shifts towards longer wavelengths with an increase of the lattice period.     

Registration of phase‐contrast images in propagation‐based X‐ray phase tomography

X‐ray phase tomography aims at reconstructing the 3D electron density distribution of an object. It offers enhanced sensitivity compared to attenuation‐based X‐ray absorption tomography. In propagation‐based methods, phase contrast is achieved by letting the beam propagate after interaction with the object. The phase shift is then retrieved at each projection angle, and subsequently used in tomographic reconstruction to obtain the refractive index decrement distribution, which is proportional to the electron density. Accurate phase retrieval is achieved by combining images at different propagation distances. For reconstructions of good quality, the phase‐contrast images recorded at different distances need to be accurately aligned. In this work, we characterise the artefacts related to misalignment of the phase‐contrast images, and investigate the use of different registration algorithms for aligning in‐line phase‐contrast images. The characterisation of artefacts is done by a simulation study and comparison with experimental data. Loss in resolution due to vibrations is found to be comparable to attenuation‐based computed tomography. Further, it is shown that registration of phase‐contrast images is nontrivial due to the difference in contrast between the different images, and the often periodical artefacts present in the phase‐contrast images if multilayer X‐ray optics are used. To address this, we compared two registration algorithms for aligning phase‐contrast images acquired by magnified X‐ray nanotomography: one based on cross‐correlation and one based on mutual information. We found that the mutual information‐based registration algorithm was more robust than a correlation‐based method.     

Selectable light‐sheet uniformity using tuned axial scanning

Light‐sheet fluorescence microscopy (LSFM) is an optical sectioning technique capable of rapid three‐dimensional (3D) imaging of a wide range of specimens with reduced phototoxicity and superior background rejection. However, traditional light‐sheet generation approaches based on elliptical or circular Gaussian beams suffer an inherent trade‐off between light‐sheet thickness and area over which this thickness is preserved. Recently, an increase in light‐sheet uniformity was demonstrated using rapid biaxial Gaussian beam scanning along the lateral and beam propagation directions. Here we apply a similar scanning concept to an elliptical beam generated by a cylindrical lens. In this case, only z‐scanning of the elliptical beam is required and hence experimental implementation of the setup can be simplified. We introduce a simple dimensionless uniformity statistic to better characterize scanned light‐sheets and experimentally demonstrate custom tailored uniformities up to a factor of 5 higher than those of unscanned elliptical beams. This technique offers a straightforward way to generate and characterize a custom illumination profile that provides enhanced utilization of the detector dynamic range and field of view, opening the door to faster and more efficient 2D and 3D imaging.     

New data‐driven method from 3D confocal microscopy for calculating phytoplankton cell biovolume

Confocal laser scanner microscopy coupled with an image analysis system was used to directly determine the shape and calculate the biovolume of phytoplankton organisms by constructing 3D models of cells. The study was performed on Biceratium furca (Ehrenberg) Vanhoeffen, which is one of the most complex‐shaped phytoplankton. Traditionally, biovolume is obtained from a standardized set of geometric models based on linear dimensions measured by light microscopy. However, especially in the case of complex‐shaped cells, biovolume is affected by very large errors associated with the numerous manual measurements that this entails. We evaluate the accuracy of these traditional methods by comparing the results obtained using geometric models with direct biovolume measurement by image analysis. Our results show cell biovolume measurement based on decomposition into simple geometrical shapes can be highly inaccurate. Although we assume that the most accurate cell shape is obtained by 3D direct biovolume measurement, which is based on voxel counting, the intrinsic uncertainty of this method is explored and assessed. Finally, we implement a data‐driven formula‐based approach to the calculation of biovolume of this complex‐shaped organism. On one hand, the model is obtained from 3D direct calculation. On the other hand, it is based on just two linear dimensions which can easily be measured by hand. This approach has already been used for investigating the complexities of morphology and for determining the 3D structure of cells. It could also represent a novel way to generalize scaling laws for biovolume calculation.     

基于线结构光的三维垂线位移测量方法

垂线测量法是观测大坝变形位移的一种简便而有效的方法,在实际大坝变形监测中得到广泛应用。 针对现有垂线坐标 仪大多只能测量垂线二维位移且结构复杂的问题,本文提出了一种基于线结构光的三维垂线测量方法。 基于垂线测量中垂线 方向不变,利用线结构光测量原理实现垂线三维位移测量。 首先,本文采用线结构光测量获取垂线二维位移,然后基于垂线上 固定标志点的成像光线和垂线相交于一点的事实,综合垂线二维测量结果和通过相机内参数恢复的固定标志点成像光线方程, 实现垂线三维位移测量。 实验结果表明,本方法在水平面内 Y 方向上的位移测量精度为±0. 1 mm,在水平面内 X 方向和竖直 Z 方向上的位移测量精度达到±0. 05 mm,测量范围为 0~ 80 mm。 相较目前垂线位移测量方法,本文方法垂线位移测量精度和测 量范围更高,且测量结构简单。     

Scaling studies with the dual crystal spectrometer at the OMEGA-EP laser facility

The dual crystal spectrometer (DCS) is an approved diagnostic at the OMEGA and the OMEGA-EP laser facilities for the measurement of high energy x-rays in the 11-90 keV energy range, e.g., for verification of the x-ray spectrum of backlighter targets of point projection radiography experiments. DCS has two cylindrically bent transmission crystal channels with image plate detectors at distances behind the crystals close to the size of the respective Rowland circle diameters taking advantage of the focusing effect of the cylindrically bent geometry. DCS, with a source to crystal distance of 1.2 m, provides the required energy dispersion for simultaneous detection of x-rays in a low energy channel (11-45 keV) and a high-energy channel (19-90 keV). A scaling study is described for varied pulse length with unchanged laser conditions (energy, focusing). The study shows that the Kα line intensity is not strongly dependent on the length of the laser pulse.     

Theoretical study of transient phenomena in near-field optics

A time-domain study of the propagation of a light pulse is made by the finite-difference time-domain method. This method is described briefly and then two applications are presented: creation and diffraction of surface plasmons in the time-domain, and propagation of a light pulse through two tip models, a dielectric one and a metal-coated one. Results on propagation speed of surface plasmons, spatio-temporal shape and spectral study of the field emitted by a tip are presented.     

Quantitative analysis of EcoR1 methylase-DNA complex by atomic force microscopy

The EcoR1 methylase specifically recognize 5'-GA* ATTC-3' in DNA duplex. We directly applied atomic force microscopy (AFM) to investigate linear pBR322-EcoR1 methylase complexes and quantitatively analyzed the bend angles of linear pBR322-EcoR1 methylase complexes and the bound protein widths. In this study, we made a novel observation that DNA-EcoR1 methylase complexes exhibited two populations of conformation at recognition site: DNA bent an acute angle at the recognition site in the presence of one EcoR1 methylase monomeric molecule, while DNA bent an unacute angle at the recognition site and the complementary site on duplex DNAs in the presence of EcoR1 methylase dimer. The data indicated that the unacute angle state was the result of unique interactions between EcoR1 methylase and the recognition site and the complementary site on duplex DNAs, and suggested that the acute angle conformation could be an intermediate in the formation of the unacute angle state. Our works provide a detail insight into the DNA structural variations involved in EcoR1 methylase-binding processes and demonstrate further the versatility of AFM as an imaging technique for studying the interaction between large DNA fragment and protein.     

Second‐harmonic microscopy of ex vivo porcine corneas

The ex vivo cornea of porcine eyes has been studied with second‐harmonic microscopy with a laboratory‐built system to examine the structure of collagen fibrils at different length scales, as well as the image dependence on polarization and wavelength of the illumination source. We found that collagen fibrils can effectively be visualized with second‐harmonic microscopy, in agreement with previous findings, at different wavelengths of the illumination. The same laser source used for imaging may also be used to induce changes to the corneal tissues that are observable both in the linear and second‐harmonic imaging channels. Such studies are essential first steps towards a future high‐resolution optical characterization technique for simultaneous corneal surgery and wound healing of the human eye.     

Magneto-optical near-field microscopy of ultrathin films in ultrahigh vacuum

In this study, guiding of surface plasmon polaritons excited at a gold film surface along corrugation‐free channels in regions that are covered with randomly located surface scatterers, is considered using near‐field microscopy for imaging of surface plasmon polariton intensity distributions at the surface. In the wavelength range 713–815 nm, we observed complete inhibition of the surface plasmon polariton propagation inside the random structures composed of individual (≈ 70 nm high) gold bumps (and their clusters) placed on a 55 nm thick gold film with a bump density of 75 µm^?2. We demonstrate well‐defined surface plasmon polariton guiding along corrugation‐free 2 µm wide channels in random structures and, in the wavelength range 738–774 nm, low‐loss guiding around 20° bends having a bend radius of ≈ 15 µm.     

一种永磁直线同步电机的有限元仿真与分析

在传统永磁直线同步电机的基础上,设计了新型永磁直线同步电机的结构,并根据电磁场的基本理论,利用Ansofi有限元分析软件进行建模,分析了电机静态时磁场和磁通密度的分布以及电机启动和稳定运行时的动态特性。研究结果为以后进一步研究此类直线电机提供了一条有效的解决问题的途径。     

Curtailed light sheet microscopy for rapid imaging of macroscopic biological specimens

We study the feasibility of volume imaging from a few angular views/scans in a light sheet fluorescence microscopy. Two‐dimensional (2D) images (angular views) were acquired at an angular separation of 10° and volume images were constructed with merely 18, 9, and 6 views. We study the structural changes in a 5‐day old Zebrafish embryo labeled with Phalloidin TRITC that binds to F‐Actin of embryo cell. To collect the data, the specimen is rotated (for varying sampling angles Δθ) with respect to a fixed vertical axis passing through the volume‐of‐interest (yolk sac). In the proposed realization of selective plane illumination microscopy (SPIM) technique, the translation is completely avoided. Analysis shows rich structural information with marginal reduction in contrast. Comparison with the state‐of‐the‐art SPIM shows appreciable volume reconstruction (from an order less 2D scans) that may be good enough for rapid monitoring of macroscopic specimens. Microsc. Res. Tech. 79:455–458, 2016. © 2016 Wiley Periodicals, Inc.     

Use of a near-field optical probe to locally launch surface plasmon polaritons on plasmonic waveguides: a study by the finite difference time domain method

We used the finite difference time domain (FDTD) method to study the use of scanning near field optical microscopy (SNOM) to locally excite the nanometric plasmonic waveguides. In our calculation, the light is funneled through a SNOM probe with a sub-wavelength optical aperture and is irradiated on one end of two types of plasmonic waveguides made of 50 nm Au sphere arrays and Au nanowires. The incident light was well localized at one end of the waveguides and consequently propagated toward the other end, due to the excitation of surface plasmon polaritons. We found that the propagation length of the nanosphere array type waveguide varies from 100 to 130 nm depending on the light wavelength, the size of the probe aperture, and the launching heights. Our result shows that reducing the aperture size and using the light of the plasmon resonance wavelength of the nanosphere array could increase the propagation length and, thus, the efficiency of electromagnetic energy transportation through nanosphere arrays.     

Shack-Hartmann wave front measurements in cortical tissue for deconvolution of large three-dimensional mosaic transmitted light brightfield micrographs

We present a novel approach for deconvolution of 3D image stacks of cortical tissue taken by mosaic/optical‐sectioning technology, using a transmitted light brightfield microscope. Mosaic/optical‐sectioning offers the possibility of imaging large volumes (e.g. from cortical sections) on a millimetre scale at sub‐micrometre resolution. However, a blurred contribution from out‐of‐focus light results in an image quality that usually prohibits 3D quantitative analysis. Such quantitative analysis is only possible after deblurring by deconvolution. The resulting image quality is strongly dependent on how accurate the point spread function used for deconvolution resembles the properties of the imaging system. Since direct measurement of the true point spread function is laborious and modelled point spread functions usually deviate from measured ones, we present a method of optimizing the microscope until it meets almost ideal imaging conditions. These conditions are validated by measuring the aberration function of the microscope and tissue using a Shack‐Hartmann sensor. The analysis shows that cortical tissue from rat brains embedded in Mowiol and imaged by an oil‐immersion objective can be regarded as having a homogeneous index of refraction. In addition, the amount of spherical aberration that is caused by the optics or the specimen is relatively low. Consequently the image formation is simplified to refraction between the embedding and immersion medium and to 3D diffraction at the finite entrance pupil of the objective. The resulting model point spread function is applied to the image stacks by linear or iterative deconvolution algorithms. For the presented dataset of large 3D images the linear approach proves to be superior. The linear deconvolution yields a significant improvement in signal‐to‐noise ratio and resolution. This novel approach allows a quantitative analysis of the cortical image stacks such as the reconstruction of biocytin‐stained neuronal dendrites and axons.     

Usefulness of hard X‐ray microscope using synchrotron radiation for the structure analysis of insects

Three‐dimensional (3D) printing technology has the advantage of enabling specific visualization of creative ideas. Since synchrotron based images can provide high sensitivity and high resolution, they are a very useful technology to identify the 3D anatomy of microscale samples. X‐ray images using such synchrotron radiation are grafted to 3D printing technology. We can be obtained 3D images and modeling data of an ant using synchrotron radiation, and then, it were outputted with the 3D printer. A new way to identify the usefulness of the structure analysis is then found by visualizing the micro‐internal structure of diverse biomedical samples and creating an enlarged model. This study suggests methods of utilizing a 3D printed model produced through synchrotron radiation imaging in various fields such as bioengineering, medical, and education.     

基于AMESim的三元催化转化器起燃特性数值模拟分析

为研究三元催化转化器(TWC)的起燃特性,采用AMESim软件建立了某车型TWC的物理模型,用数值求解气固两相的质量守恒方程组和能量守恒方程组的方法对其进行了模拟计算,并得到了不同影响因数下的CO、氮氧化合物(NOx)、碳氢化合物(CaHb)等废气转化率曲线。结果表明:发动机控制策略、TWC的位置及其内部结构对起燃特性都有影响。     

Quality of a three-dimensional image stimulating eye accommodation

A 3D image in the form of a linear combination of two two-dimensional images located at different depths along the line of vision of the eye is considered. The loss of contrast and nonlinearity of accommodation over the depth with a high resolution acuity of 3D images are demonstrated in computer experiments with different eye models.     

A line-scanning semi-confocal multi-photon fluorescence microscope with a simultaneous broadband spectral acquisition and its application to the study of the thylakoid membrane of a cyanobacterium Anabaena PCC7120

We describe the construction and characterization of a laser‐line‐scanning microscope capable of detection of broad fluorescence spectra with a resolution of 1 nm. A near‐infrared femtosecond pulse train at 800 nm was illuminated on a line (one lateral axis, denoted as X axis) in a specimen by a resonant scanning mirror oscillating at 7.9 kHz, and total multi‐photon–induced fluorescence from the linear region was focused on the slit of an imaging polychromator. An electron‐multiplying CCD camera was used to resolve fluorescence of different colours at different horizontal pixels and fluorescence of different spatial positions in a specimen at different vertical pixels. Scanning on the other two axes (Y and Z) was achieved by a closed‐loop controlled sample scanning stage and a piezo‐driven objective actuator. The full widths at half maximum of the point‐spread function of the system were estimated to be 0.39–0.40, 0.33 and 0.56–0.59 μm for the X (lateral axis along the line‐scan), Y (the other lateral axis) and Z axes (the axial direction), respectively, at fluorescence wavelengths between 644 and 690 nm. A biological application of this microscope was demonstrated in a study of the sub‐cellular fluorescence spectra of thylakoid membranes in a cyanobacterium, Anabaena PCC7120. It was found that the fluorescence intensity ratio between chlorophyll molecules mainly of photosystem II and phycobilin molecules of phycobilisome (chlorophyll/phycobilin), in the thylakoid membranes, became lower as one probed deeper inside the cells. This was attributable not to position dependence of re‐absorption or scattering effects, but to an intrinsic change in the local physiological state of the thylakoid membrane, with the help of a transmission spectral measurement of sub‐cellular domains. The efficiency of the new line‐scanning spectromicroscope was estimated in comparison with our own point‐by‐point scanning spectromicroscope. Under typical conditions of observing cyanobacterial cells, the total exposure time became shorter by about 50 times for a constant excitation density. The improvement factor was proportional to the length of the line‐scanned region, as expected.     

Numerical study on thermal stress cutting of silicon wafers using two-line laser beams

We propose a method of cleaving silicon wafers using two-line laser beams. The base principle is separating the silicon wafer using crack propagation caused by laser-induced thermal stress. Specifically, this method uses two-line laser beams parallel to the cutting line such that the movements of the laser beam along the cutting line can be omitted, which is necessary when using a point beam. To demonstrate the proposed method, 3D numerical analysis of a heat transfer and thermo-elasticity model was performed. Crack propagation was evaluated by comparing the stress intensity factor (SIF) at the crack tip with the fracture toughness of silicon, where crack propagation is assumed begin when the SIF exceeds the fracture toughness. The influences of laser power, line beam width, and distance between two laser beams were also investigated. The simulation results showed that the proposed method is appropriate for cleaving silicon wafers without any thermal damage.