Lagrangian mass-flow investigations of inorganic contaminants in wastewater-impacted streams

Understanding the potential effects of increased reliance on wastewater treatment plant (WWTP) effluents to meet municipal, agricultural, and environmental flow requires an understanding of the complex chemical loading characteristics of the WWTPs and the assimilative capacity of receiving waters. Stream ecosystem effects are linked to proportions of WWTP effluent under low-flow conditions as well as the nature of the effluent chemical mixtures. This study quantifies the loading of 58 inorganic constituents (nutrients to rare earth elements) from WWTP discharges relative to upstream landscape-based sources. Stream assimilation capacity was evaluated by Lagrangian sampling, using flow velocities determined from tracer experiments to track the same parcel of water as it moved downstream. Boulder Creek, Colorado and Fourmile Creek, Iowa, representing two different geologic and hydrologic landscapes, were sampled under low-flow conditions in the summer and spring. One-half of the constituents had greater loads from the WWTP effluents than the upstream drainages, and once introduced into the streams, dilution was the predominant assimilation mechanism. Only ammonium and bismuth had significant decreases in mass load downstream from the WWTPs during all samplings. The link between hydrology and water chemistry inherent in Lagrangian sampling allows quantitative assessment of chemical fate across different landscapes.     

Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance

To provide the first nationwide reconnaissance of the occurrence of pharmaceuticals, hormones, and other organic wastewater contaminants (OWCs) in water resources, the U.S. Geological Survey used five newly developed analytical methods to measure concentrations of 95 OWCs in water samples from a network of 139 streams across 30 states during 1999 and 2000. The selection of sampling sites was biased toward streams susceptible to contamination (i.e. downstream of intense urbanization and livestock production). OWCs were prevalent during this study, being found in 80% of the streams sampled. The compounds detected represent a wide range of residential, industrial, and agricultural origins and uses with 82 of the 95 OWCs being found during this study. The most frequently detected compounds were coprostanol (fecal steroid), cholesterol (plant and animal steroid), N,N-diethyltoluamide (insect repellant), caffeine (stimulant), triclosan (antimicrobial disinfectant), tri(2-chloroethyl)phosphate (fire retardant), and 4-nonylphenol (nonionic detergent metabolite). Measured concentrations for this study were generally low and rarely exceeded drinking-water guidelines, drinking-water health advisories, or aquatic-life criteria. Many compounds, however, do not have such guidelines established. The detection of multiple OWCs was common for this study, with a median of seven and as many as 38 OWCs being found in a given water sample. Little is known about the potential interactive effects (such as synergistic or antagonistic toxicity) that may occur from complex mixtures of OWCs in the environment. In addition, results of this study demonstrate the importance of obtaining data on metabolites to fully understand not only the fate and transport of OWCs in the hydrologic system but also their ultimate overall effect on human health and the environment.     

Survey of organic wastewater contaminants in biosolids destined for land application

In this study, the presence, composition, and concentrations of organic wastewater contaminants (OWCs) were determined in solid materials produced during wastewater treatment. This study was undertaken to evaluate the potential of these solids, collectively referred to as biosolids, as a source of OWCs to soil and water in contact with soil. Nine different biosolid products, produced by municipal wastewater treatment plants in seven different states, were analyzed for 87 different OWCs. Fifty-five of the OWCs were detected in at least one biosolid product. The 87 different OWCs represent a diverse cross section of emerging organic contaminants that enter wastewater treatment plants and may be discharged without being completely metabolized or degraded. A minimum of 30 and a maximum of 45 OWCs were detected in any one biosolid. The biosolids used in this study are produced by several production methods, and the plants they originate from have differing population demographics, yet the percent composition of total OWC content, and of the most common OWCs, typically did not vary greatly between the biosolids tested. The summed OWC content ranged from 64 to 1811 mg/kg dry weight. Six biosolids were collected twice, 3-18 months apart, and the total OWC content of each biosolid varied by less than a factor of 2. These results indicate that the biosolids investigated in this study have OWC compositions and concentrations that are more similar than different and that biosolids are highly enriched in OWCs (as mass-normalized concentrations) when compared to effluents or effluent-impacted water. These results demonstrate the need to better describe the composition and fate of OWCs in biosolids since about 50% of biosolids are land applied and thus become a potentially ubiquitous nonpoint source of OWCs into the environment.     

Reproductive disruption in fish downstream from an estrogenic wastewater effluent

To assess the impact of an estrogenic wastewater treatment plant (WWTP) effluent on fish reproduction, white suckers (Catostomus commersoni) were collected from immediately upstream and downstream (effluent site) of the city of Boulder, CO, WWTP outfall. Gonadal intersex, altered sex ratios, reduced gonad size, disrupted ovarian and testicular histopathology, and vitellogenin induction consistent with exposure to estrogenic wastewater contaminants were identified in white suckers downstream from the WWTP outfall and not at the upstream site. The sex ratio was female-biased at the effluent site in both the fall of 2003 and the spring of 2004; the frequency of males at the effluent site (17-21%) was half that of the upstream site (36-46%). Intersex white suckers comprised 18-22% of the population at the effluent site. Intersex fish were not found at the upstream site. Chemical analyses determined that the WWTP effluent contained a complex mixture of endocrine-active chemicals, including 17beta-estradiol (E2) 17alpha-ethynylestradiol, alkylphenols, and bisphenol A resulting in an estimated total estrogen equivalence of up to 31 ng E2 L(-1). These results indicate that the reproductive potential of native fishes may be compromised in wastewater-dominated streams.     

Ab initio and in situ comparison of caffeine, triclosan, and triclocarban as indicators of sewage-derived microbes in surface waters

Three organic wastewater compounds (OWCs) were evaluated in theory and practice for their potential to trace sewage-derived microbial contaminants in surface waters. The underlying hypothesis was that hydrophobic OWCs outperform caffeine as a chemical tracer, due to their sorptive association with suspended microorganisms representing particulate organic carbon (POC). Modeling from first principles (ab initio) of OWC sorption to POC under environmental conditions suggested an increasing predictive power: caffeine (0.2% sorbed) < triclosan (9-60%; pH 6-9) < triclocarban (76%). Empirical evidence was obtained via analysis of surface water from three watersheds in a rural-to-urban gradient in Baltimore, MD. Mass spectrometric OWC detections were correlated to microbial plate counts for 40 monitoring sites along 14 streams, including multiple chronic sewage release sites and the local wastewater treatment plant. Consistent with ab initio calculations, correlation analyses of 104 observations for fecal coliforms, enterococci, and Escherichia coli in natural surface waters showed that the particle-active antimicrobials triclosan and triclocarban (R2 range, 0.45-0.55) were indeed superior to caffeine (0.16-0.37) for tracking of microbial indicators. It is concluded that chemical monitoring of microbial risks is more effective when using hydrophobic OWCs such as triclosan and triclocarban in place of, or in conjunction with, the traditional marker caffeine.     

Occurrence and fate of organic contaminants during onsite wastewater treatment

Onsite wastewater treatment systems serve approximately 25% of the U.S. population. However, little is known regarding the occurrence and fate of organic wastewater contaminants (OWCs), including endocrine disrupting compounds, during onsite treatment. A range of OWCs including surfactant metabolites, steroids, stimulants, metal-chelating agents, disinfectants, antimicrobial agents, and pharmaceutical compounds was quantified in wastewater from 30 onsite treatment systems in Summit and Jefferson Counties, CO. The onsite systems represent a range of residential and nonresidential sources. Eighty eight percent of the 24 target compounds were detected in one or more samples, and several compounds were detected in every wastewater sampled. The wastewater matrices were complex and showed unique differences between source types due to differences in water and consumer product use. Nonresidential sources generally had more OWCs at higher concentrations than residential sources. Additional aerobic biofilter-based treatment beyond the traditional anaerobic tank-based treatment enhanced removal for many OWCs. Removal mechanisms included volatilization, biotransformation, and sorption with efficiencies from <1% to >99% depending on treatment type and physicochemical properties of the compound. Even with high removal rates during confined unit onsite treatment, OWCs are discharged to soil dispersal units at loadings up to 20 mg/m2/d, emphasizing the importance of understanding removal mechanisms and efficiencies in onsite treatment systems that discharge to the soil and water environments.     

Fish endocrine disruption responses to a major wastewater treatment facility upgrade

The urban-water cycle modifies natural stream hydrology, and domestic and commercial activities increase the burden of endocrine-disrupting chemicals, such as steroidal hormones and 4-nonylphenol, that can disrupt endocrine system function in aquatic organisms. This paper presents a series of integrated chemical and biological investigations into the occurrence, fate, and effects of endocrine-disrupting chemicals in the City of Boulder Colorado's WWTF and Boulder Creek, the receiving stream. Results are presented showing the effects of a full-scale upgrade of the WWTF (that treats 0.6 m(3) s(-1) of sewage) from a trickling filter/solids contact process to an activated sludge process on the removal of endocrine-disrupting compounds and other contaminants (including nutrients, boron, bismuth, gadolinium, and ethylenediaminetetraacetic acid) through each major treatment unit. Corresponding impacts of pre- and postupgrade effluent chemistry on fish reproductive end points were evaluated using on-site, continuous-flow experiments, in which male fathead minnows (Pimephales promelas) were exposed for 28 days to upstream Boulder Creek water and WWTF effluent under controlled conditions. The upgrade of the WWTF resulted in improved removal efficiency for many endocrine-disrupting chemicals, particularly 17β-estradiol and estrone, and fish exposed to the postupgrade effluent indicated reduction in endocrine disruption relative to preupgrade conditions.     

Aqueous stability of gadolinium in surface waters receiving sewage treatment plant effluent, Boulder Creek, Colorado

In many surface waters, sewage treatment plant (STP) effluent is a substantial source of both regulated and unregulated contaminants, including a suite of complex organic compounds derived from household chemicals, pharmaceuticals, and industrial and medical byproducts. In addition, STP effluents in some urban areas have also been shown to have a positive gadolinium (Gd) anomaly in the rare earth element (REE) pattern, with the Gd derived from its use in medical facilities. REE concentrations are relatively easy to measure compared to many organic wastewater compounds and may provide a more widely utilized tracer of STP effluents. To evaluate whether sewage treatment plant-associated Gd is a useful tracer of treatment plant effluent, an investigation of the occurrence, fate, and transport of rare earth elements was undertaken. The rare earth element patterns of four of five STP effluents sampled display positive Gd anomalies. The one site that did not have a Gd anomaly serves a small community, population 1200, with no medical facilities. Biosolids from a large metropolitan STP are not enriched in Gd even though the effluent is, suggesting that a substantial fraction of Gd remains in the aqueous phase through routine treatment plant operation. To evaluate whether STP-derived Gd persists in the fluvial environment, a 14-km study reach downstream of an STP was sampled. Gadolinium anomalies were present at all five downstream sites, but the magnitude of the anomaly decreased. Effluent from STPs is a complex mixture of organic and inorganic constituents, and to better understand the chemical interactions and their effect on REEs, the aqueous speciation was modeled using comprehensive chemical analyses of water samples collected downstream of STP input. These calculations suggest that the REEs will likely remain dissolved because phosphate and carbonate complexes dominate over free REE ions. This study supports the application of Gd anomalies as a useful tracer of urban wastewater.     

Accumulation of contaminants in fish from wastewater treatment wetlands

Increasing demands on water resources in arid environments make reclamation and reuse of municipal wastewater an important component of the water budget. Treatment wetlands can be an integral part of the water-reuse cycle providing both water-quality enhancement and habitat functions. When used for habitat, the bioaccumulation potential of contaminants in the wastewater is a critical consideration. Water and fish samples collected from the Tres Rios Demonstration Constructed Wetlands near Phoenix, Arizona, which uses secondary-treated wastewater to maintain an aquatic ecosystem in a desert environment, were analyzed for hydrophobic organic compounds (HOC) and trace elements. Semipermeable membrane devices (SPMD) were deployed to investigate uptake of HOC. The wetlands effectively removed HOC, and concentrations of herbicides, pesticides, and organic wastewater contaminants decreased 40-99% between inlet and outlet. Analysis of Tilapia mossambica and Gambusia affinis indicated accumulation of HOC, including p,p'-DDE and trans-nonachlor. The SPMD accumulated the HOC detected in the fish tissue as well as additional compounds. Trace-element concentrations in whole-fish tissue were highly variable, but were similar between the two species. Concentrations of HOC and trace elements varied in different fish tissue compartments, and concentrations in Tilapia liver tissue were greater than those in the whole organism or filet tissue. Bioconcentration factors for the trace elements ranged from 5 to 58,000 and for the HOC ranged from 530 to 150,000.     

Transport of chemical and microbial compounds from known wastewater discharges: potential for use as indicators of human fecal contamination

The quality of drinking and recreational water is currently (2005) determined using indicator bacteria. However, the culture tests used to analyze forthese bacteria require a long time to complete and do not discriminate between human and animal fecal material sources. One complementary approach is to use chemicals found in human wastewater, which would have the advantages of (1) potentially shorter analysis times than the bacterial culture tests and (2) being selected for human-source specificity. At 10 locations, water samples were collected upstream and at two successive points downstream from a wastewaster treatment plant (WWTP); a treated effluent sample was also collected at each WWTP. This sampling plan was used to determine the persistence of a chemically diverse suite of emerging contaminants in streams. Samples were also collected at two reference locations assumed to have minimal human impacts. Of the 110 chemical analytes investigated in this project, 78 were detected at least once. The number of compounds in a given sample ranged from 3 at a reference location to 50 in a WWTP effluent sample. The total analyte load at each location varied from 0.018 microg/L at the reference location to 97.7 microg/L in a separate WWTP effluent sample. Although most of the compound concentrations were in the range of 0.01-1.0 microg/L, in some samples, individual concentrations were in the range of 5-38 microg/L. The concentrations of the majority of the chemicals present in the samples generally followed the expected trend: they were either nonexistent or at trace levels in the upstream samples, had their maximum concentrations in the WWTP effluent samples, and then declined in the two downstream samples. This research suggests that selected chemicals are useful as tracers of human wastewater discharge.     

Attenuation of wastewater-derived contaminants in an effluent-dominated river

Although wastewater-derived chemical contaminants undergo transformation through a variety of mechanisms, the relative importance of processes such as biotransformation and photolysis is poorly understood under conditions representative of large rivers. To assess attenuation rates under conditions encountered in such systems, samples from the Trinity River were analyzed for a suite of wastewater-derived contaminants during a period when wastewater effluent accounted for nearly the entire flow of the river over a travel time of approximately 2 weeks. While the concentration of total adsorbable organic iodide, a surrogate for recalcitrant X-ray phase contrast media in wastewater, was approximately constant throughout the river, concentrations of ethylenediamine tetraacetate, gemfibrozil, ibuprofen, metoprolol, and naproxen all decreased between 60% and 90% as the water flowed downstream. Comparison of attenuation rates estimated in the river with rates measured in laboratory-scale microcosms suggests that biotransformation was more important than photolysis for most of the compounds. Further evidence for biotransformation in the river was provided by measurements of the enantiomeric fraction of metoprolol, which showed a gradual decrease as the water moved downstream. Results of this study indicate that natural attenuation can result in significant decreases in concentrations of wastewater-derived contaminants in large rivers.     

Positive effects of wastewater ozonation displayed by in situ bioassays in the receiving stream

Tertiary treatment methods, like ozonation, are currently under discussion to improve removal efficiencies of micropollutants by municipal wastewater treatment plants (WWTPs). In order to assess the effects of a full-scale wastewater ozonation at WWTP Wu?eri, Switzerland, on the receiving stream, a total of seven in situ bioassays with Gammarus fossarum that lasted 7-days were conducted during an overall period of 33 months. Caged gammarids were exposed between 150 m up- and 400 m downstream of a WWTP effluent before, during and following the operation of the full-scale wastewater ozonation. During the release of nonozone treated wastewater, gammarid feeding was significantly reduced by up to 90% 50 and 150 m downstream of the WWTP effluent. In contrast, during the operation period of the ozonation, no significant alterations in feeding were observed downstream. The results of these bioassays were incorporated into a mathematical simulation of the release of nonozone treated wastewater in a Central European region, suggesting a 40% reduction in leaf litter breakdown and hence in energy provision for the remaining aquatic food web downstream of WWTP effluents, while the release of ozone treated wastewater did not affect this important ecosystem function.     

Fate of pesticides in the arid subtropics, Botswana, Southern Africa

Despite a history of pesticide usage, few data exist on their concentrations in air and soil of Southern Africa. To add to the understanding of the processes controlling the fate of organic contaminants in arid regions, the levels, spatial trends, and seasonal variability of pesticides were studied in air and soil from Botswana. XAD resin-based passive air samplers (PAS) were deployed at 15 sites across the country from May 2006 to May 2007. Soil samples were collected from the vicinity of nine of the PAS sampling sites. In addition, 27 24-h high-volume air samples were collected in Maun, at the southeastern edge of the Okavango Delta, every two weeks for one year. Levels of pesticides in PAS were low, with α-endosulfan and lindane being most abundant. Concentrations in soils were extremely low and only soils with high organic carbon contained notable amounts of dieldrin and traces of other pesticides. In particular, air and soil from the Okavango Delta had very low levels even though the area had repeatedly been sprayed with DDT and endosulfan in the past. Air samples from Eastern Botswana, where the majority of the population lives, contained higher levels. Higher air concentrations of α-endosulfan occurred during summer and higher HCB levels occurred in winter. This seasonality was related with neither minor seasonal changes in temperature nor hydrological seasonal events such as the rainy season or the flooding of the Okavango Delta. Thus, the observed spatial and seasonal patterns are more likely related to pesticide usage pattern than to environmental factors or historical use. High temperature and low organic matter content limit the uptake capacity of most subtropical soils for pesticides. No evidence was found that sorption to dry mineral matter plays a major role. Arid soils in subtropical regions are therefore neither a major reservoir of organic contaminants nor do they constitute a significant long-term source of pesticides to the atmosphere.     

Aerosol-bound emissions of polycyclic aromatic hydrocarbons and sterols from aeration tanks of a municipal waste water treatment plant

Aeration tanks of wastewater treatment plants (WWTP) are a potential source of atmospheric aerosol particles. Several groups of organic compounds (sterols, polycyclic aromatic hydrocarbons, estrogens) were analyzed in aerosol particles sampled at a municipal WWTP, and the particle size distribution was measured directly with optical particle counters. Aerosol emissions from an activated treatment tank equipped with fine bubble diffusers were low; however, at the preaeration tank equipped with coarse bubble diffusers, sterol concentrations up to 14 ng m(-3) were measured. Directly next to the tank, sterols were associated mainly to particles with aerodynamic diameter >1.35 microm. The results suggest that coprostanol could be a useful tracer for monitoring the emission of aerosol particles from WWTPs. Moreover, wastewater treatment could contribute substantially to the atmospheric concentrations of cholesterol and 24-ethylcholesterol. Aeration tanks with fine bubble diffusers are no major source of atmospheric aerosol particles, whereas coarse bubbling devices seem to emit considerable amounts of aerosol particles.     

Consequences of treated water recycling as regards pharmaceuticals and drugs in surface and ground waters of a medium-sized Mediterranean catchment

In Mediterranean regions where the population is rapidly growing, the risk of water resource contamination by wastewater is likely to increase. This is the case of the Hérault watershed (south of France), where the presence of treated wastewater in surface and ground waters has been shown in a previous study. To assess the consequence of these wastewater contaminations as regards pharmaceuticals and other organic compounds, 16 common pharmaceuticals (amitryptilin, acetylsalicylic acid, carbamazepine, clenbuterol, diazepam, diclofenac, doxepin, gemfibrozil, ibuprofen, imipramine, ketoprofen, naproxen, nordiazepam, paracetamol, salbutamol, and terbutalin) as well as wastewater related pollutants (caffeine, gadolinium anomaly, and boron) were analyzed in wells pumped for potable water supply and in two wastewater treatment plant (WWTP) effluents. In addition, a monitoring along the Lergue River (the main tributary of the Hérault River) was achieved to assess pharmaceutical behavior in surface waters. Pharmaceuticals and other wastewater-related contaminants are present in several reservoirs tapped for drinking water, confirming wastewater contamination; paracetamol, caffeine, and diclofenac are the most frequently detected. Paracetamol is present at rather high concentrations (up to 11 microg/L and 211 ng/L, respectively, in a wastewater effluent and in a drinking water sample). Though degradable in WWTP, caffeine is commonly encountered in surface waters and detected in highly polluted groundwater. On the contrary, acetylsalicylic acid concentrations are generally low despite a large consumption in France; this is related to its metabolism in humans and rapid degradation in the aquatic environment. The monitoring of pharmaceuticals along the Lergue River shows that dilution is sufficient to decrease pharmaceutical values.     

Sterols and anionic surfactants in urban aerosol: emissions from wastewater treatment plants in relation to background concentrations

Aerosol particles that are emitted from aeration tanks of wastewater treatment plants (WWTPs) can be enriched with environmentally relevant wastewater constituents. In this study, aerosol particles were sampled simultaneously at the pre-aeration tank of a municipal WWTP and at two urban locations approximately 1 km away from the WWTP to evaluate the significance of these aerosol emissions. Moreover, aerosol particles were sampled at a small wastewater irrigation facility and at a rural site. In aerosol particles and wastewater, six sterols (cholesterol, coprostanol, campesterol, beta-sitosterol, stigmasterol, stigmastanol) and anionic surfactants (expressed in terms of methylene blue active substances, MBAS) were quantified. The results showed significantly higher concentrations of sterols and MBAS at the WWTP than atthe urban locations. At the WWTP, average concentrations of cholesterol (848 +/- 321 pg m(-3)), coprostanol (1132 +/- 565 pg m(-3)), and MBAS (132 +/- 43 ng m(-3)) in aerosol were approximately twice as high. This can be attributed to emissions from the treatment tank. Coprostanol, a unique tracer for wastewater, was detected only occasionally at the urban locations. The results of this study show that the aeration of wastewater is a continuously operating local source for organic compounds in aerosol. The wastewater irrigation facility was a minor source of aerosol-bound sterols (coprostanol, 287 +/- 218 pg m(-3)) and anionic surfactants (64 +/- 32 ng m(-3)). Except for coprostanol, all compounds were also present in samples of rural aerosol.     

Impact of reclaimed water on select organic matter properties of a receiving stream-fluorescence and perylene sorption behavior

Surface water samples obtained from the Bull Run tributary upstream and downstream of the Upper Occoquan Sewage Authority(UOSA) advanced wastewater reclamation facility (WRF) were characterized by fluorescence excitation-emission matrix (EEM) spectroscopy, and sorption coefficients (Kmoc) of macromolecular organic carbon isolates were quantified by fluorescence quenching. The EEM data revealed a signature fluorescence distribution in the downstream samples that was attributed to the presence of proteinlike material. The Kmoc values for upstream samples were consistently and significantly higher than those of corresponding downstream samples. There was a moderate correlation (R2 = 0.67) between log Kmoc and the molar extinction coefficient at 280 nm (E280) and a strong correlation (R2 = 0.96) between Kmoc and the proteinlike fluorescence region for macromolecular isolates with negligible quantum yields. This study demonstrates that organic matter downstream of the UOSA-WRF has unique fluorescence and perylene sorption characteristics compared to those of upstream organic matter during summer baseflow conditions. This implies that wastewater treatment facilities, including those advanced facilities designed to reclaim wastewater for indirect potable reuse, can influence the composition and behavior of organic matter in a receiving stream.     

Persistent organic pollutants in fish: biomonitoring and cocktail effect with implications for food safety

The impact of anthropogenic wastes of persistent organic pollutants (POPs) on the marine environment has increased in the last decades. POPs include polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCs). To assess the levels of these POPs in the wild fish population, pelagic and benthopelagic predator fish species were selected as biomonitors. For detection and quantification of POPs in muscular tissues, a simple extraction through Accelerated-Solvent-Extraction (ASE) with an ‘in-line’ clean up purification approach was applied, followed by a GC–MS/MS analysis. Concentrations of sum DDT, sum HCH and endrin correlated with all PCB concentrations. Significant differences among fish species were found for all OCs and all PCBs except PCB 31 and 101. Blackspot seabream had the highest PCB concentrations; OCs were highest in tuna. Due to major concerns regarding fish population losses and the possible human chronic exposure to contaminated fish, studies addressing combined effects of multiple POPs (‘cocktail effect’) should be implemented. Our data motivate further experimental and observational studies in fish to define adequate baseline levels for cumulative human exposure and potential role of these contaminants for food safety.     

Removal of sulfur-organic polar micropollutants in a membrane bioreactor treating industrial wastewater

While membrane bioreactors (MBR) have proven their large potential to remove bulk organic matter from municipal as well as industrial wastewater, their suitability to remove poorly degradable polar wastewater contaminants is yet unknown. However, this is an important aspect for the achievable effluent quality and in terms of wastewater reuse. We have analyzed two classes of polar sulfur-organic compounds, naphthalene sulfonates and benzothiazoles, by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS) over a period of 3 weeks in the influent and effluent of a full-scale MBR with external ultrafiltration that treats tannery wastewater. While naphthalene monosulfonates were completely removed, total naphthalene disulfonate removal was limited to about 40%, and total benzothiazoles concentration decreased for 87%. Quantitative as well as qualitative data did not indicate an adaptation to or a more complete removal of these polar aromatic compounds by the MBR as compared to literature data on conventional activated sludge treatment. While quality improvements in receiving waters for bulk organic matter are documented and the same can be anticipated for apolar particle-associated contaminants, these data provide no indication that MBR will improve the removal of polar poorly biodegradable organic pollutants.