共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
5.
Persistency of Enlarged Autolysosomes Underscores Nanoparticle‐Induced Autophagy in Hepatocytes 下载免费PDF全文
Ji‐Qian Zhang Wei Zhou Sha‐Sha Zhu Jun Lin Peng‐Fei Wei Fen‐Fen Li Pei‐Pei Jin Han Yao Yun‐Jiao Zhang Yi Hu Yi‐Ming Liu Ming Chen Zheng‐Quan Li Xue‐sheng Liu Li Bai Long‐ping Wen 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(7)
The diverse biological effects of nanomaterials form the basis for their applications in biomedicine but also cause safety issues. Induction of autophagy is a cellular response after nanoparticles exposure. It may be beneficial in some circumstances, yet autophagy‐mediated toxicity raises an alarming concern. Previously, it has been reported that upconversion nanoparticles (UCNs) elicit liver damage, with autophagy contributing most of this toxicity. However, the detailed mechanism is unclear. This study reveals persistent presence of enlarged autolysosomes in hepatocytes after exposure to UCNs and SiO2 nanoparticles both in vitro and in vivo. This phenomenon is due to anomaly in the autophagy termination process named autophagic lysosome reformation (ALR). Phosphatidylinositol 4‐phosphate (PI(4)P) relocates onto autolysosome membrane, which is a key event of ALR. PI(4)P is then converted into phosphatidylinositol 4,5‐bisphosphate (PI(4,5)P2) by phosphatidylinositol‐4‐phosphate 5‐kinase. Clathrin is subsequently recruited by PI(4,5)P2 and leads to tubule budding of ALR. Yet it is observed that PI(4)P cannot be converted in nanoparticle‐treated hepatocytes cells. Exogenous supplement of PI(4,5)P2 suppresses the enlarged autolysosomes in vitro. Abolishment of these enlarged autolysosomes by autophagy inhibitor relieves the hepatotoxicity of UCNs in vivo. The results provide evidence for disrupted ALR in nanoparticle‐treated hepatocytes, suggesting that the termination of nanoparticle‐induced autophagy is of equal importance as the initiation. 相似文献
6.
7.
Lingxiang Wang Liang Wang Xiangju Meng Feng‐Shou Xiao 《Advanced materials (Deerfield Beach, Fla.)》2019,31(50)
Supported metal nanoparticles are widely used as catalysts in the industrial production of chemicals, but still suffer from deactivation because of metal leaching and sintering at high temperature. In recent years, serious efforts have been devoted to developing new strategies for stabilizing metal nanoparticles. Recent developments for preparing sinter‐resistant metal‐nanoparticle catalysts via strong metal–support interactions, encapsulation with oxide or carbon layers and within mesoporous materials, and fixation in zeolite crystals, are briefly summarized. Furthermore, the current challenges and future perspectives for the preparation of highly efficient and extraordinarily stable metal‐nanoparticle‐based catalysts, and suggestions regarding the mechanisms involved in sinter resistance, are proposed. 相似文献
8.
Franziska Lübkemann Jan Frederick Miethe Frank Steinbach Pascal Rusch Anja Schlosser Dniel Zmb Thea Heinemeyer Dominik Natke Dorian Zok Dirk Dorfs Nadja C. Bigall 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(39)
Nanoparticle‐based voluminous 3D networks with low densities are a unique class of materials and are commonly known as aerogels. Due to the high surface‐to‐volume ratio, aerogels and xerogels might be suitable materials for applications in different fields, e.g. photocatalysis, catalysis, or sensing. One major difficulty in the handling of nanoparticle‐based aerogels and xerogels is the defined patterning of these structures on different substrates and surfaces. The automated manufacturing of nanoparticle‐based aerogel‐ or xerogel‐coated electrodes can easily be realized via inkjet printing. The main focus of this work is the implementation of the standard nanoparticle‐based gelation process in a commercial inkjet printing system. By simultaneously printing semiconductor nanoparticles and a destabilization agent, a 3D network on a conducting and transparent surface is obtained. First spectro‐electrochemical measurements are recorded to investigate the charge–carrier mobility within these 3D semiconductor‐based xerogel networks. 相似文献
9.
Abraham S. Moses Olena R. Taratula Hyelim Lee Fangzhou Luo Tanner Grenz Tetiana Korzun Anna St Lorenz Fahad Y. Sabei Shay Bracha Adam W. G. Alani Ov D. Slayden Oleh Taratula 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(18)
Endometriosis is a painful disorder where endometrium‐like tissue forms lesions outside of the uterine cavity. Intraoperative identification and removal of these lesions are difficult. This study presents a nanoplatform that concurrently delineates and ablates endometriosis tissues using real‐time near‐infrared (NIR) fluorescence and photothermal therapy (PTT). The nanoplatform consists of a dye, silicon naphthalocyanine (SiNc), capable of both NIR fluorescence imaging and PTT, and a polymeric nanoparticle as a SiNc carrier to endometriosis tissue following systemic administration. To achieve high contrast during fluorescence imaging of endometriotic lesions, nanoparticles are constructed to be non‐fluorescent prior to internalization by endometriosis cells. In vitro studies confirm that these nanoparticles activate the fluorescence signal following internalization in macaque endometrial stromal cells and ablate them by increasing cellular temperature to 53 °C upon interaction with NIR light. To demonstrate in vivo efficiency of the nanoparticles, biopsies of endometrium and endometriosis from rhesus macaques are transplanted into immunodeficient mice. Imaging with the intraoperative Fluobeam 800 system reveals that 24 h following intravenous injection, nanoparticles efficiently accumulate in, and demarcate, endometriotic grafts with fluorescence. Finally, the nanoparticles increase the temperature of endometriotic grafts up to 47 °C upon exposure to NIR light, completely eradicating them after a single treatment. 相似文献
10.
11.
12.
Key Role of TFEB Nucleus Translocation for Silver Nanoparticle‐Induced Cytoprotective Autophagy 下载免费PDF全文
Jun Lin Yiming Liu Hao Wu Zhihai Huang Jingfan Ma Chang Guo Feng Gao Peipei Jin Pengfei Wei Yunjiao Zhang Liu Liu Rui Zhang Longxin Qiu Ning Gu Longping Wen 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(13)
13.
14.
Paolo Della Vedova Mirolyuba Ilieva Vitaliy Zhurbenko Ramona Mateiu Adele Faralli Martin Dufva Ole Hansen 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(2):248-256
A novel molecular beacon (a nanomachine) is constructed that can be actuated by a radio frequency (RF) field. The nanomachine consists of the following elements arranged in molecular beacon configuration: a gold nanoparticle that acts both as quencher for fluorescence and a localized heat source; one reporter fluorochrome, and; a piece of DNA as a hinge and recognition sequence. When the nanomachines are irradiated with a 3 GHz RF field the fluorescence signal increases due to melting of the stem of the molecular beacon. A control experiment, performed using molecular beacons synthesized by substituting the gold nanoparticle by an organic quencher, shows no increase in fluorescence signal when exposed to the RF field. It may therefore be concluded that the increased fluorescence for the gold nanoparticle‐conjugated nanomachines is not due to bulk heating of the solution, but is caused by the presence of the gold nanoparticles and their interaction with the RF field; however, existing models for heating of gold nanoparticles in a RF field are unable to explain the experimental results. Due to the biocompatibility of the construct and RF treatment, the nanomachines may possibly be used inside living cells. In a separate experiment a substantial increase in the dielectric losses can be detected in a RF waveguide setup coupled to a microfluidic channel when gold nanoparticles are added to a low RF loss liquid. This work sheds some light on RF heating of gold nanoparticles, which is a subject of significant controversy in the literature. 相似文献
15.
16.
17.
Trifolium‐like Platinum Nanoparticle‐Mediated Photothermal Therapy Inhibits Tumor Growth and Osteolysis in a Bone Metastasis Model 下载免费PDF全文
Changping Wang Xiaopan Cai Jishen Zhang Xinyu Wang Yu Wang Huyifeng Ge Wangjun Yan Quan Huang Jianru Xiao Qiang Zhang Yiyun Cheng 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(17):2080-2086
Bone metastasis is a frequent and fatal complication of cancer that lacks effective clinical treatment. Photothermal therapy represents a new strategy for the destruction of multiple cancers. In this study, trifolium‐like platinum nanoparticles (TPNs) with small size and excellent photothermal conversion property are prepared via a facile and green method. TPNs show minimal cytotoxicity on normal cell lines and kill cancer cells upon exposure to a near‐infrared light. These nanoparticles effectively inhibit tumor growth and prevent osteolysis in a bone metastasis model. This study offers a promising strategy in the treatment of bone metastasis. 相似文献
18.
19.