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1.
Methyl-branched fatty acids, which are usually minor components (≤0.1%) in fish oils, were concentrated in the non-urea-complexing
fraction along with polyunsaturated fatty acids during the enrichment of omega-3 fatty acids from certain fish oils via the
urea complexation process. The methyl-branched fatty acids in the omega-3 polyunsaturated fatty acid concentrates, which were
prepared from three fish body oils, were characterized by gas chromatography and gas chromatography/mass spectrometry. Among
the major branched-chain fatty acids expected and identified were the known isoprenoid acids—mainly 4,8,12-trimethyltridecanoic,
pristanic, and phytanic—and the well-known iso and anteiso structures. Two novel phytol-derived multimethyl-branched fatty
acids, 2,2,6,10,14-pentamethylpentadecanoic and 2,3,7,11,15-pentamethylhexadecanoic, were identified in redfish (Sebastes sp.) oil. These two fatty acids were absent in oils from menhaden (Brevoortia tyrannus) and Pacific salmon (mixed, but mostly from sockeye,Oncorhynchus nerka). The major branched-chain fatty acid in the salmon oil, 7-methyl-7-hexadecenoic acid, was also present to a moderate extent
in menhaden oil. A novel vicinal dimethyl-branched fatty acid, 7,8-dimethyl-7-hexadecenoic was detected in all of the fish
oils examined, but was most important in the salmon oil. Three monomethyl-branched fatty acids, 11-methyltetradecanoic acid,
and 11- and 13-methylhexadecanoic, hitherto undescribed in fish lipids, were also detected in salmon, redfish and menhaden
oils.
Presented in part at the First Annual Meeting of the Amer. Oil. Chem. Soc. Canadian Section, Guelph, Ontario, Oct. 8–9, 1986. 相似文献
2.
M. F. King L. C. Boyd B. W. Sheldon 《Journal of the American Oil Chemists' Society》1992,69(6):545-551
The antioxidant properties of phospholipids (PL) in a refined salmon oil model system were measured by determining changes
in the 2-thiobarbituric acid number and decreases in the ratio of docosahexaenoic acid (DHA)/palmitic acid (22:6/16:0) of
a fish oil system incubated at 180°C for up to 3 h. The more phosphatidylcholine (PC) added to the oil system, the higher
the oxidative stability obtained. The order of effectiveness of commercial phospholipids in inhibiting oxidation and the loss
of polyunsaturated fatty acids was as follows: sphingomyelin (SPH)=lysophosphatidylcholine (LPC)=phosphatidylcholine (PC)=phosphatidylethanolamine
(PE)>phosphatidylserine (PS)>phosphatidylinositol (PI)>phosphatidylglycerol (PG)>control salmon oil. Nitrogen containing PL,
including PE, PC, LPC and SPH, were equally effective in exerting greater antioxidant properties than PS, PG and PI. The inverse
relationship observed between the oxidation index (C22:6/C16:0) and color intensity for treatments following 2 h of heating
suggests that Maillard-type reaction products may have contributed to the oxidative stability of PL-supplemented fish oils. 相似文献
3.
M. Zlatanov S. Ivanov K. Aitzetmüller 《European Journal of Lipid Science and Technology》1999,101(11):437-439
The phospholipid and fatty acid composition of three Bulgarian nut oils were investigated. Phospholipids were separated by Folch′s method and two-directional thin-layer chromatography. Their content was determined spectrophotometrically. Phospholipids were present at levels of 0.8% in almond oil, 2.8% in hazelnut oil, and 0.9% in walnut oil. Phosphatidylcholine (18—50%), phosphatidylinositol (18—45%), and phosphatidylethanolamine (8—16%) were found to be the major components. Small amounts of phosphatidylserine, phosphatidic acids, phosphatidylglycerols, lysophosphatidylcholine, and lysophosphatidylethanolamine were also detected. The fatty acid composition of glyceride oils and of the four main phospholipids, namely phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidic acids was identified by capillary gas chromatography of their methyl esters. The predominant fatty acid present in almond and hazelnut oils was linoleic (83.2% and 80.8%, respectively). Oleic acid (18.7%), linoleic acid (48.5%), and linolenic acid (15.8%) were the major components in walnut oil. Higher quantities of saturated fatty acids (27.8—81.2%) were found to be in the phospholipids than in the corresponding oils (9.5—16.7%). 相似文献
4.
The oil content and fatty acid composition of commercially important Turkish fish species (anchovy,Engraulis encrasicholus; freshwater rainbow trout,Salmo gairdneri; and cultured salmon,S. salar) were determined. Palmitic (16∶0), palmitoleic (16∶1), oleic (18∶1), and docosahexaenoic (22∶6) acids were the most abundant
fatty acids in all species. Eicosapentaenoic acid (20∶5) was twice as high in the anchovy oil as in the rainbow trout and
salmon oils. Significant quantities of linoleic acid (18∶2) and docosahexaenoic acids (22∶6) were found in both rainbow trout
and salmon samples. The individual fatty acid data obtained from rainbow trout and salmon were similar to each other. All
three fish species contain high levels of n-3 polyunsaturated fatty acids and would be suitable for inclusion in the formulation
of low-fat highly unsaturated diets. 相似文献
5.
High fat diets promote the development of mammary tumors induced in rats by 7,12-dimethylbenz(a)anthracene (DMBA), and polyunsaturated
fats are more effective than saturated fats. This difference is related to the linoleic acid content of polyunsaturated vegetable
oils, but the amount of linolealte required for maximum tumor promotion appears to be higher than indicated by earlier experiments.
Comparison of the effects of a polyunsaturated vegetable oil (corn oil) containing linoleate with a fish oilo (menhaden oil)
containing polyunsaturated fatty acids derived from linolenic acid showed that higher dietary mammary tumors, while corresponding
levels of menhaden oil had an inhibitory effect. This is further evidence that promotion of mammary tumorigenesis by polyunsaturated
vegetable oils may be mediated by prostaglandins or other biologically active eicosanoids derived from n−6 fatty acids. 相似文献
6.
Juvenile coho salmon were injected intraperitoneally with [1-14C] linolenic acid, and sampled at 24, 120, and 240 hr. Liver, heart, and gill lipids were extracted, analyzed, and halflives
of individual liver glycerophospholipids and n-3 fatty acids determined from rates of loss of radioactivity. Incorporation
of label into gill was much less than into either heart or liver. Total acyl halflife was shorter for the choline phospholipids
than for the ethanolamine phospholipids, as were the halflives of all individual n-3 fatty acids. Eicosapentaenoic acid (20∶5n-3)
had the shortest halflife in both phospholipids (50–60 hr), while docosapentaenoic acid (22∶5n-3) and docosahexaenoic acid
(22∶6n-3) had much longer halflives. Specific activities of the shorter chain n-3 fatty acids were much greater than the longer,
more unsaturated homologs at all times, suggesting possible differences in their mechanisms of incorporation into phospholipids.
Diacylglycerol analysis indicated that de novo synthesis could be responsible for the incorporation of only a small portion
of the labeled long chain fatty acids found in phospholipids. The fatty acid halflives reported here for salmon are in general
agreement with those found previously in mammals.
Technical Paper No. 5238, Oregon Agricultural Experiment Station, Oregon State University, Corvallis, Oregon 97331.
This material is taken in part from a thesis submitted in partial fulfillment of the requirements for the MS degree in 1978. 相似文献
7.
Lipase-assisted concentration of n-3 polyunsaturated fatty acids in acylglycerols from marine oils 总被引:1,自引:0,他引:1
Udaya N. Wanasundara Fereidoon Shahidi 《Journal of the American Oil Chemists' Society》1998,75(8):945-951
Preparation of n-3 polyunsaturated fatty acid (PUFA) concentrates from seal blubber oil (SBO) and menhaden oil (MHO) in the
form of acylglycerols was carried out by hydrolysis with a number of commercial microbial lipases. The lipases tested were
Aspergillus niger, Candida cylindracea (CC), Chromobacterium viscosum, Geotrichum candidum, Mucor miehei, Pseudomonas sp., Rhizopus oryzae, and Rhizopus niveus. After lipase-assisted hydrolysis of oils, free fatty acids were removed, and fatty acid composition of the mixture containing
mono-, di-, and triacylglycerols was determined. All lipases were effective in increasing the n-3 PUFA content of the remaining
acylglycerols of both SBO and MHO. The highest concentration of n-3 PUFA was provided by CC lipase; 43.5% in SBO [9.75% eicosapentaenoic
acid (EPA), 8.61% docosapentaenoic acid (DPA), and 24.0% docosahexaenoic acid (DHA)] and 44.1% in MHO (18.5% EPA, 3.62% DPA,
and 17.3% DHA) after 40 h of hydrolysis. Thus, CC lipase appears to be most suitable for preparation of n-3 PUFA in the acylglycerol
form from marine oils. 相似文献
8.
T. D. Simpson 《Journal of the American Oil Chemists' Society》1989,66(9):1309-1311
Phospholipids in isolated soybean lipid bodies at 30‡C underwent degradation by acyl ester and phosphodiester hydrolysis and
by phosphatidyl transfer. Under conditions that minimized oxidation, preferential loss of phosphatidylethanolamine and polyunsaturated
fatty acids occurred, but acyl ester compositions of all phospholipids reflected enrichment of saturated and/or monounsaturated
acids. Such fatty acid changes in phosphatidylinositol, which was degraded less than phosphatidylcholine or phosphatidylethanolamine,
and in phosphatidic acid, which accumulated, also suggest that transesterification occurred extensively in lipid bodies. The
specific temporal changes in phospholipid and acyl chain composition suggest that several enzymes remained active in isolated
lipid bodies. 相似文献
9.
Atlantic salmon post-smolts were fed diets containing either fish oils (Fosol, FO and Marinol, MO) rich in long-chain n-3
polyunsaturated fatty acids (PUFA), or plant oils rich in 18:2n-6 (sunflower oil, SO) or 18:3n-3 (linseed oil, LO) for 12
wk. The major PUFA in individual phospholipids from gill and kidney were related to the dietary lipid intake. Levels of n-6
PUFA were highest while levels of n-3 PUFA were lowest in fish fed SO. Fish fed LO generally had lower levels of 20:4n-6 compared
to the other treatments while fish fed SO generally had the highest levels of 20:4n-6. In all phospholipid classes except
phosphatidylinositol (PI) 20:5n-3 was greatest in fish fed MO followed by FO, LO, and SO. In PI, 20:5n-3 was also highest
in fish fed MO but those fed LO contained more 20:5n-3 than those fed FO. This resulted in the ratio of the eicosanoid precursors,
20:4n-6/20:5n-3, being significantly greater in fish fed SO, for all phospholipid classes, compared to fish fed the other
three dietary oils. The activity of gill phospholipase A was greatest in fish fed FO and was lowest in fish fed SO. The concentration
of PGF3α was significantly increased in gill homogenates from fish fed MO compared to the other three treatments while PGF2α was significantly increased in fish fed SO compared to those fed LO. The concentration of PGE3 was significantly reduced in kidney homogenates from fish fed SO compared to the other three treatments while PGE2 was significantly increased in fish fed SO compared to those fed either FO or LO. 相似文献
10.
Long-chain polyunsaturated (n-3) fatty acids have been proposed to be involved in a wide variety of biological activities.
In this study, mitochondrial docosahexaenoic acid (DHA) levels were increased by either dietary manipulation or by fusing
the mitochondria with phospholipid vesicles made from 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (18:0/22:6 PC). The fused mitochondria exhibited a DHA-induced decrease in respiratory control index
(RCI) and membrane potential and an increase in proton movement. The modified mitochondria also demonstrated an increase in
fluidity (as detected by 1,6-diphenyl-1,3,5-hexatriene anisotropy) and changes in membrane structure detected by the fluorescence
probes MC540 and pyrene decanoate. Proton movement in lipid vesicles made from mitochondrial lipid extracts was shown to be
enhanced by incorporated 18:0/22:6 PC. Mitochondria were isolated from young (5-mon) and old (24-mon) mice which were maintained
on either a diet rich in saturated fats (hydrogenated coconut oil) or rich in n−3 polyunsaturated fats (menhaden oil). Mitochondrial
bioenergetic function was followed by RCI, state 3 respiration, ATP level, and phosphate uptake. In addition, lipid composition,
phospholipid area/molecule, and extent of lipid peroxidation were also determined. Decreases in RCI for the menhaden oil diet-modified
mitochondria paralleled those in which DHA levels were enhanced by fusion with phospholipid vesicles. RCI reductions are attributed
to DHA-induced increases in H+ movement, producing diminished mitochondrial membrane potentials. One purpose of this project was to determine if the deleterious
effects of aging on mitochondrial bioenergetic function could be reversed by addition of n−3 fatty acids. The experiments
reported here indicate that incorporation of long-chain polyunsaturated n−3 fatty acids into mitochondrial membranes does
not appear likely to reverse the effects of age on mitochondrial function. 相似文献
11.
Cholesterol gallstone formation in the prairie dog is accompanied by an increase in the percentage of biliaryphospholipids containing arachidonic acid, and an increase in gallbladder prostaglandin (PG) synthesis, but the pathogenetic significance of these
changes is unclear. Dietary supplementation with eicosapentaenoic acid (EPA), an omega-3 fatty acid which is commonly found
in fish oil, decreases prostaglandin synthesis in some tissues by replacing arachidonic acid, and by competitively inhibiting
prostaglandin synthesis. We studied the effect of dietary fish oil on gallbladder PG synthesis, and the relative abundance
of various molecular species of phosphatidylcholines and phosphatidylethanolamines in bile and gallbladder epithelium in the
cholesterol-fed prairie dog. Prairie dogs were maintained for 4 weeks on one of four diets: i) control, ii) cholesterol-supplemented
(0.34%), iii) menhaden oil (50 g/kg chow), or iv) cholesterol plus menhaden oil. Supplementation with menhaden oil resulted
in a replacement of arachidonic and linoleic acids with EPA and docosahexaenoic acids in the phospholipids of bile and gallbladder
mucosa. In cholesterol-fed animals, supplementation with menhaden oil prevented increased gallbladder PG synthesis. Menhaden
oil also reduced the incidence of cholesterol monohydrate crystals among cholesterol-fed animals (9/20 with cholesterol plus
menhaden oil vs 21/22 with cholesterol alone), but the improvement could not clearly be attributed to decreased PG synthesis
since supplementation with menhaden oil also increased the total phospholipid concentration in bile, and decreased the degree
of cholesterol saturation. These results demonstrate that dietary supplementation with omega-3 fatty acids significantly influences
biliary phospholipids, and decreases the incidence of cholesterol monohydrate crystal formation in this animal model. 相似文献
12.
We have studied the effect of various diets on the phospholipid fatty acid composition andin vitro Δ5 desaturase activity of hepatic microsomes derived either from the normal or streptozotocin-induced diabetic rat. The diets
studied were the standard rat chow diet and a basal fat-free diet supplemented either with 20 percent saturated fat, 20 percent
unsaturated fat, or 20 percent menhaden oil. Phospholipid fatty acid composition analysis revealed that the normal rat fed
the saturated fat or menhaden oil diet had significantly decreased arachidonate levels, consistent with decreased Δ5 desaturase
activities and decreased 18∶2n−6 intake. On the contrary, the unsaturated fat diet decreased dihomo-γ-linolenate and increased
arachidonate levels, without increased Δ5 desaturase activity. Streptozotocininduced diabetes resulted in decreased arachidonate
and Δ5 desaturase activity. The unsaturated fat diet fed to the diabetic rat also failed to correct this decreased Δ5 desaturase
activity. The unsaturated fatty acids in this diet also displaced a substantial amount of n−3 fatty acids in both normal and
diabetic microsomes, due to the competition between these two fatty acid families for incorporation into the membrane phospholipids.
Conversely, the menhaden oil diet fed to the normal and diabetic rats displaced n−6 fatty acids, reduced Δ5 desaturase activity,
and enhanced 22∶6n−3 incorporation into diabetic microsomes. 相似文献
13.
Sepideh Haghighat Kharazi Reza Esmaeilzadeh Kenari Zeinab Raftani Amiri Maryam Azizkhani 《Journal of the American Oil Chemists' Society》2012,89(7):1241-1247
The aim of this study is to describe the physicochemical properties of Iranian virgin olive oil (Zard, Mari and Phishomi)
cultivated in Roodbar, Gilan. There were statistically significant differences for most of the parameters (P < 0.05). The acidity and peroxide value were in the limit established for classification as extra virgin olive oil. The oil
of Zard had the highest amount of monounsaturated fatty acids followed by Mari and Phishomi oils. Mari oil proved to have
the minimum value of polyunsaturated fatty acids, and the highest amount of phenolic compounds and oxidative stability. The
oil of Phishomi had the maximum amount of chlorophylls and carotenoids and therefore it had the highest color index. There
were no significant differences between the cultivars regarding the refractive index (1.469 at 20 °C for all three cultivars).
According to the high content of monounsaturated fatty acids, the lowest amounts of polyunsaturated fatty acids and the highest
amounts of phenolic compounds as well as the results of a Rancimat assay, it seems that the quality of the oil of Mari cultivar
is better than Zard and Phishomi oils and is also more stable against oxidation. 相似文献
14.
The antioxidant effects of oil‐in‐water nanoemulsion based on edible citrus peel essential oils on the fatty acid composition of rainbow trout fillets stored at 4 ± 2 °C are investigated. Fish fillets are treated with nanoemulsion and stored for 16 days. Lipid samples are converted into fatty acid methyl esters which are then detected by gas chromatagrophy (GC). The results show that palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), vaccenic acid (C18:1?‐7), oleic acid (C18:1?9), eicosenoic acid (C20:1?9), linoleic acid (C18:2?6), linolenic acid (C18:3?3), eicosapentaenoic acid (EPA) (C20:5?3), and docosahexaenoic acid (DHA) (C22:6?3) are the most important fatty acids in fish meat. While polyene index and hypocholesterolemic:hypercholesterolaemic fatty acid ratios decrease in trout fillets during cold storage, thrombogenicity index and atherogenicity index generally increase (especially in control and Tween 80 groups). The concentrations of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) are higher in the treatment groups and the saturated fatty acids (SFAs) are lower in all groups compared to those of the control group. Application of nanoemulsion based on citrus essential oils prevents oxidation of PUFA especially EPA and DHA, thus has potential as a preservative for fish oil. Practical Applications: In recent years, nanotechnological applications have been increasingly applied to the protection of food. Similarly, natural essential oils are used to increase the shelf life of foods. This study demonstrates the combined effect of a new method of nanoemulsions and essential oils on the safety of foods. 相似文献
15.
Edward Siguel 《Lipids》1996,31(1):S51-S56
Dietary and plasma fatty acids have been linked to total cholesterol but not to the ratio of total/high-density lipoprotein
cholesterol (TC/HDLC). To evaluate the relationship between dietary and plasma levels of polyunsaturated fatty acids (PUFA)
and TC/HDLC, we analyzed cross-sectional and longitudinal data using 519 plasma samples (50% men, 50% women) from subjects
participating in the Framingham Heart Study and results from a study feeding diets rich in either n-6 linoleic acid or n-3
α-linolenic acid with or without fish oil supplements (n-3 derivatives). Values of TC/HDLC are inversely related to the percent
of plasma PUFA when both variables are measured at the same time in different subjects,R=0.82,P<0.000001. PUFA in phospholipids increase in response to increased dietary intake of different PUFA, either n-3 or n-6 or
fish oils. There was a highly significant inverse relationship between TC/HDLC and the percent of PUFA in phospholipids,R=0.97,P<0.001. The relationship was similar regardless of the source and type of dietary fatty acids. A similar relationship existed
when only the baseline points were considered. When plasma PUFA % increases, either in response to a diet high in PUFA or
across different subjects, TC/HDLC ratios decline. Evaluation of plasma fatty acid profiles and increased balanced dietary
intake of PUFA to bring fatty acid profiles of subjects with low PUFA plasma levels closer to the profile of a healthy reference
group is an effective approach to reduce high TC/HDLC. Reductions of more than 50% in TC/HDLC appear feasible with dietary
modification alone. Further research into fatty acid metabolic activity may determine the biochemical basis of common dysplipidemias. 相似文献
16.
Transesterifications were investigated to determine a means for preparing polyunsaturated phospholipids simply from soy phospholipid,
sardine oil, and two kinds of microbial lipases originating fromCandida cylindracea andRhizopus delemar.
The optimum reaction conditions forCandida cylindracea lipase were: 4 g of sardine oil, 10 mL of water, 0.7 g of lipase, 10 mL of hexane, 48 hr of reaction time at 37°C for 3 g
of soy phospholipid, for which the transesterification ratio reached approximately 45%. Recovery of phospholipid was low,
because hydrolysis also occurred under these reaction conditions. However, hydrolysis could be suppressed by using glycerine
instead of water, and the recovery of phospholipid increased to 47%, although the transesterification ratio was reduced to
32%.
Rhizopus delemar lipase has 1,3-specificity for triglycerides, and the transesterification ratio was approximately 37% in the 1-position of
phospholipid. The resulting phospholipid was rich in polyunsaturated fatty acids and linoleic acid, while the total percentage
of polyunsaturated fatty acids incorporated was 18.4%. Therefore, polyunsaturated phospholipids can be prepared easily by
transesterification of soy phospholipid with fish oil by means of commercial lipases. 相似文献
17.
Yasuhiro Ando Toru Ota Yukiko Matsuhira Kazunaga Yazawa 《Journal of the American Oil Chemists' Society》1996,73(4):483-487
This paper presents the positional distribution of fatty acids in docosahexaenoic acid (22∶6n-3)-rich fish oil triacyl-sn-glycerols (TG). Stereospecific analysis of TG was carried out by a nonenzymatic method. The TG of bonito head oil, obtained
after a winterization process, contained 22∶6n-3 at concentrations of 28,7, and 49 mole % in thesn-1,sn-2, andsn-3 positions, respectively. In the TG of oil before the winterization process, 22∶6n-3 was concentrated in thesn-3 position, followed evenly by thesn-1 andsn-2 positions. Tuna orbital oil, obtained after winterization, showed the preferential association of 22∶6n-3 to thesn-3 position, followed by thesn-1 position. This distribution pattern was similar to that observed for seal oil TG rather than sardine oil TG. The bonito
head and tuna orbital oils are useful as fish oils with characteristics different from those of common fish oils, such as
menhaden, sardine, and herring oils. 相似文献
18.
Cessation of polyunsaturated fatty acid formation in four selected filamentous fungi when grown on plant oils 总被引:6,自引:0,他引:6
Four fungi,Conidiobolus nanodes, Entomophthora exitalis, Mortierella isabellina, andMucor circinelloides, were grown on various oils (triolein, sesame, safflower, linseed, and oil fromM. isabellina) and produced lipids in which the fatty acids were predominantly the same as those of the original staring substrate. Only
in the first two cases was there evidence of a small amount of chain elongation and of fatty acid desaturation taking place.
The extent of this was only about 10% of that seen in glucose-grown cells. The apparent repression of the fatty acid desaturases
and elongases was not reversed by growing cells on glucose and oils as mixed substrates—the fatty acid profiles were the same
as when the fungi had grown in oils alone. Neither was the cessation of polyunsaturated fatty acid synthesis due to the presence
of nonoil components (NOC) in the oil. Only the NOC from sesame oil affected one single conversion, that of 20∶3n-3 to 20∶4n–6.
We conclude that fatty acid desaturase and elongase systems are repressed either partially or completely in a filamentous
fungi grown on triacylglycerol oils. 相似文献
19.
Meriam Tir Imen El Cafsi Rym B. Ammar Hssan Mejri Nizar Tlili Mhamed El Cafsi 《Lipids》2019,54(2-3):163-176
The aim of the present work was to study the effect of season on phospholipids and triacylglycerols (TAG) of mantle and tentacles of female and male wild Sepia officinalis. The identified phospholipids were phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), phosphatidylserine (PtdSer), and phosphatidylinositol (PtdIns), and PtdEtn was the major fraction. Results showed apparent seasonal variation of phospholipid content, particularly with female samples. Fatty acid composition of phospholipid classes showed a differentiation much more in the proportions than in the diversity of fatty acids. Results showed that the major saturated fatty acids were 16:0 and 18:0, the major monounsaturated fatty acids were 18:1 and 20:l, and the major polyunsaturated fatty acids were docosahexaenoic acid (22:6n-3) (DHA) and eicosapentaenoic acid (20:5n-3) (EPA). The results relative to TAG demonstrated significant variations. Principal component analysis confirmed the seasonal and sexual effects. This study could be appropriate for the improvement of consistent monitoring of phospholipid and TAG accumulation in cephalopod, which might be important for both physiological studies and food industries. 相似文献
20.
Effects of dietary triacylglycerol structure on triacylglycerols of resultant chylomicrons from fish oil- and seal oil-fed rats 总被引:3,自引:0,他引:3
We investigated the influence of the intramolecular fatty acid distribution of dietary triacyl-sn-glycerols (TAG) rich in n-3 polyunsaturated fatty acids (PUFA) on the structure of chylomicron TAG. Fish oil and seal oil,
comparable in fatty acid compositions but with different contents of major n-3 PUFA esterified at thesn-2 position (20:5n-3, 46.6%, and 5.3%; 22:6n-3, 75.5%, and 3.8%, respectively), were fed to rats. Mesenteric lymph was collected
and the chylomicrons were isolated by ultracentrifugation. The fatty acid composition of chylomicrons largely reflected the
fatty acid composition of the oils administered. The intramolecular fatty acid distributions of the TAG fed were reflected
in the chylomicron TAG as the fraction of the total contents observed in thesn-2 position of 20:5n-3 were 23.6 and 13.3%, and of 22:6n-3 were 30.6 and 5.4% for resultant chylomicrons following fish oil
and seal oil administration, respectively. Thus, after seal oil administration, significant higher load of n-3 PUFA was esterified
in thesn-1,3 positions of chylomicron TAG compared with fish oil administration (P<0.05). 相似文献