硫氰酸钾分光光度法快速测定多维铁口服液的铁含量

目的建立硫氰酸钾分光光度法快速测定多维铁口服液中铁含量的分析方法。方法样品经酸化氧化处理,考察了硫氰酸钾分光光度法的最大吸收波长、显色剂的最佳用量、最佳显色时间、精密度和加标回收率,并与国标法进行了比较。结果方法的最大吸收波长为480 nm,最佳显色剂用量为1.00 m L 50%的硫氰酸钾溶液,最佳显色时间是10 min,方法线性回归方程为Y=0.12037X+0.00357,相关系数为0.99988,检出限为0.020μg/m L,回收率为99.5%～100.94%,相对标准偏差为0.15%～0.48%。结论与国标法比较,该方法具有良好的精密度、准确度和重复性,且成本较低、易于控制、操作简单、快速、稳定等优点。     

铁(Ⅲ)-磺基水杨酸光度法测定柚肉中的V_C

Fe(Ⅲ)与磺基水杨酸在p H=2~3的溶液中形成紫红色配离子,加入适量的VC将溶液中的Fe3+还原成Fe2+,溶液吸光度减小。测定溶液加入VC前后的吸光度分别记为A0、A,其吸光度变化为ΔA=A0-A。以ΔA与VC浓度CVc绘制标准工作曲线。在VC浓度4μg/m L~37μg/m L的范围内,ΔA(y)与CVc(x)的线性回归方程为y=0.018 2x+0.027 1,相关系数为r=0.999 6。利用该法测得梅县沙田柚果肉VC的含量为97.92 mg/100 g,测定相对标准偏差为1.1%,测定回收率95.6%~98.9%(n=5)。     

功能食品中铁含量现场快速检测方法的建立

建立功能食品中铁快速检测技术。通过试验选择最佳反应条件。曲拉通-100为增溶剂三价铁与硫氰酸钾显色反应,形成红色络合物。该络合物在波长480 nm,使用AMMS-1型快检仪检测。三价铁浓度在0.5 mg/L～3.0 mg/L范围内,呈线性关系。相关系数为0.999 4,最低检出限为50μg/kg本方法摩尔吸光系数为3.6×104 L/(mol.cm),标准偏差2.4%。该方法用于测定功能食品中铁含量,操作简捷,结果准确可靠。     

双波长分光光度法测定杨梅叶中总黄酮的研究

以超声波法辅助乙醇提取杨梅叶试样中的总黄酮,采用双波长分光光度法测定提取液的黄酮浓度。结果表明,在测定波长415 nm,参比波长475 nm条件下,芦丁标准品的标准曲线为ΔA=0.025 9c+0.002 5,芦丁标准品的质量浓度在3.7~37.1 mg/L与ΔA呈良好的线性关系(R~2=0.999 7),检出限为0.1 mg/g,测定结果的相对标准偏差(n=6)为2.46%,加标回收率在95.27%~101.35%,建立的试验方法具有良好的准确度及精密度。     

三波长分光光度法测定桑叶中总黄酮的研究

建立了三波长分光光度法测定桑叶中总黄酮的方法。结果表明,以Al(NO_3)_3为显色剂,测定波长为465,508和604 nm,在0.012~0.040 mg/m L质量浓度范围内,芦丁质量浓度与其吸光度差ΔA呈良好线性关系,回归方程为ΔA=5.918 1c-0.002 1,R~2=0.998 4。方法的回收率在95.03%~101.38%之间,RSD为2.59%。测得桑叶中总黄酮含量为19.24 mg/g。该方法操作简便,稳定性好,为桑叶总黄酮的开发和检测提供了依据。     

木质活性炭中铁含量测定的探讨

比较了二价铁和三价铁作为标准物质,采用1,10菲啰啉分光光度法测定木质活性炭中铁的含量。使用三价铁作为标准溶液,与国家标准(GB/T 12496.19-1999)使用二价铁作为标准溶液测定木质活性炭中铁含量的检验方法的测定结果具有很高的一致性。方法相对标准偏差为1.41%,准确度较高,适用于木质活性炭中铁含量的分析。     

甲基红褪色光度法测定自来水中的二氧化氯

探讨以二氧化氯(ClO2)氧化甲基红(MR)褪色光度法测定自来水中ClO2的新方法。在c(MR):c(ClO2)=6.4:1、pH4.0～4.5、温度25℃、反应时间40min条件下,以λmax=518nm测定ΔA518。结果其线性方程为ΔA518=-1.7845c(ClO2)+1.5228(r=0.992),表观摩尔吸光系数ε=1.2×105L/(mol.cm),适用测定范围为0.0004～0.80mg/L,DL为0.0036mg/L;ClO2氧化MR褪色反应具有一级反应的特点,在25℃的动力学方程为ln[c∞/(c∞-c)]=-0.000689t+2.263669、Ea为87.3kJ/mol、t1/2为16.8min。本法经自来水和消毒剂中ClO2含量的测定结果表明,相对标准偏差为2.43%～3.83%,加标回收率为98.2%～103.2%。经F检验和t检验,本法与对氨基-N,N-二乙基苯胺(DPD)光度法不存在显著差异(P=0.05),测定结果符合分析的要求。     

分光光度法测定酱油中微量铁

阿拉伯树胶存在下,在pH 5.0 NaAc-HAc缓冲溶液中,铁(Ⅱ)与邻菲啰啉(phen)和亮黄(BY)反应生成稳定的离子缔合物[Fe(phen)3]BY,在离子缔合物的最大吸收峰478 nm处体系吸光度明显增强,建立了分光光度法测定微量铁的新方法.试验了酸度、试剂用量、增效剂、反应温度和反应时间的影响,确定了最佳反应条件.铁(Ⅱ)浓度在0～1.0 μg/mL范围内符合比耳定律,表观摩尔吸光系数为2.95×104L/mol/cm,检出限为20.44 μg/L.方法用于测定酱油中铁含量,结果与原子吸收分光光度法一致,相对标准偏差分别为1.1％和0.8％(n=5),平均回收率分别为100.5％和98.3％.     

可见分光光度法评价食品机械用润滑油的润滑品质

食品机械润滑油的润滑性能直接影响机械的磨损程度,进而影响机械的使用寿命。而机械齿轮上的铁元素又是磨损过程中容易剥落的,测定模拟使用后的润滑油中铁元素的磨损量,比较其润滑品质。通过采用分光光度法研究铁含量的最佳试验条件:最大吸收波长为512 nm,10%盐酸羟胺溶液2.50 m L,HAc-Na Ac缓冲溶液(pH 4.7),0.15%邻菲罗啉溶液4.00 m L,显色时间20 min。线性范围为0~2.0μg/m L,符合朗波比尔定律,相关系数r=0.999 4,摩尔吸光系数为1.5×104 L·mol~(-1)·cm~(-1)。对样品分析表明:相对标准偏差分别为0.6%,4.7%,4.3%和3.2%,平均加标回收率97.3%~101.3%,符合国标要求。以该方法清楚得出润滑油的铁磨损量变化趋势,相比得出KLUBER oil4 UH1~(-1)5润滑品质最佳,而大豆植物油润滑品质不佳。     

分光光度法测定酵母抽取物中的铁含量

研究铁(Ⅱ)与邻菲啰啉和刚果红的络合反应,建立分光光度法测定铁含量的新方法.通过试验优化酸度、邻菲啰啉用量、刚果红用量、反应温度和反应时间等测定条件.结果表明:最大吸收波长为540 nm,表观摩尔吸光系数为4.4×104 L/mol·cm,铁(Ⅱ)浓度在0～0.8μg/mL范围内符合比尔定律,检出限为8.8μg/L.该方法用于测定酵母抽取物中的铁含量,结果与国标法一致,相对标准偏差为0.48％～0.77％(n=5),回收率为98.2％～101.6％.     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press